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  • 99
    Bio-Rad cfx96 real time pcr detection system
    Bland-Altman plot comparing the artus cytomegalovirus (CMV) RG <t>PCR</t> kit and the Real-Q CMV Quantification kit on different real-time PCR platforms: (A) the 7500 Fast Real-time PCR system, (B) the <t>CFX96</t> real-time PCR detection system. Solid lines are the mean differences between the values; dashed lines are the mean difference plus or minus 1.96 SD (95% confidential interval of mean difference).
    Cfx96 Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 28811 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad cfx96 real time system
    Transcript analysis of PGE2 receptors in THP-1 macrophages during S. Typhimurium and Y. enterocolitica infection. THP-1 macrophages (2.5 × 10 5 ) were infected for 2 h with (A) S. Typhimurium or (B) Y. enterocolitica at an MOI of 50:1. Total RNA was extracted using a PureLink RNA mini kit, and cDNA was generated using the iScript system (Bio-Rad). RT-PCR was performed using SYBR green on a <t>CFX96</t> Real-Time System (Bio-Rad) targeting prostanoid receptors EP1, EP2, EP3, EP4, and GAPDH as a reference gene. Resulting data were then normalized to GAPDH mRNA levels, and uninfected samples served as a baseline for fold change determination using the ΔΔCt method. Resulting data is representative of three biological replicates and three technical replicates and represents the mean fold change ± SD. One-way ANOVA test with Tukey’s multiple testing correction was used to establish statistical significance. p -values were indicated as follows: ∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001; ∗∗∗∗ p ≤ 0.0001.
    Cfx96 Real Time System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 23624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfx96 real time system/product/Bio-Rad
    Average 93 stars, based on 23624 article reviews
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    Bio-Rad cfx96 touch real time pcr detection system
    Decision tree flowchart developed using the interpretation rules for the triplex real-time <t>PCR</t> assay run on a Bio-Rad <t>CFX96</t> system.
    Cfx96 Touch Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15776 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad cfx96 thermocycler
    Decision tree flowchart developed using the interpretation rules for the triplex real-time <t>PCR</t> assay run on a Bio-Rad <t>CFX96</t> system.
    Cfx96 Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1831 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad cfx96 touchtm real time pcr detection system
    Decision tree flowchart developed using the interpretation rules for the triplex real-time <t>PCR</t> assay run on a Bio-Rad <t>CFX96</t> system.
    Cfx96 Touchtm Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad cfx96 real time system c1000 thermal cycler
    Decision tree flowchart developed using the interpretation rules for the triplex real-time <t>PCR</t> assay run on a Bio-Rad <t>CFX96</t> system.
    Cfx96 Real Time System C1000 Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 925 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bio-Rad cfx96 qpcr system
    Decision tree flowchart developed using the interpretation rules for the triplex real-time <t>PCR</t> assay run on a Bio-Rad <t>CFX96</t> system.
    Cfx96 Qpcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 639 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Bland-Altman plot comparing the artus cytomegalovirus (CMV) RG PCR kit and the Real-Q CMV Quantification kit on different real-time PCR platforms: (A) the 7500 Fast Real-time PCR system, (B) the CFX96 real-time PCR detection system. Solid lines are the mean differences between the values; dashed lines are the mean difference plus or minus 1.96 SD (95% confidential interval of mean difference).

    Journal: Annals of Laboratory Medicine

    Article Title: Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood

    doi: 10.3343/alm.2016.36.6.603

    Figure Lengend Snippet: Bland-Altman plot comparing the artus cytomegalovirus (CMV) RG PCR kit and the Real-Q CMV Quantification kit on different real-time PCR platforms: (A) the 7500 Fast Real-time PCR system, (B) the CFX96 real-time PCR detection system. Solid lines are the mean differences between the values; dashed lines are the mean difference plus or minus 1.96 SD (95% confidential interval of mean difference).

    Article Snippet: In the present study, we assessed the performance of the Real-Q assay for quantifying CMV DNA in WB using two real-time PCR platforms: the 7500 Fast real-time PCR system (7500 Fast; Applied Biosystems, Foster City, CA, USA) and the CFX96 real-time PCR detection system (CFX96; Bio-Rad, Hercules, CA, USA).

    Techniques: Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Linearity of the Real-Q cytomegalovirus (CMV) Quantification kit on different real-time PCR platforms: (A) the 7500 Fast real-time PCR system and (B) the CFX96 real-time PCR detection system.

    Journal: Annals of Laboratory Medicine

    Article Title: Performance Evaluation of the Real-Q Cytomegalovirus (CMV) Quantification Kit Using Two Real-Time PCR Systems for Quantifying CMV DNA in Whole Blood

    doi: 10.3343/alm.2016.36.6.603

    Figure Lengend Snippet: Linearity of the Real-Q cytomegalovirus (CMV) Quantification kit on different real-time PCR platforms: (A) the 7500 Fast real-time PCR system and (B) the CFX96 real-time PCR detection system.

    Article Snippet: In the present study, we assessed the performance of the Real-Q assay for quantifying CMV DNA in WB using two real-time PCR platforms: the 7500 Fast real-time PCR system (7500 Fast; Applied Biosystems, Foster City, CA, USA) and the CFX96 real-time PCR detection system (CFX96; Bio-Rad, Hercules, CA, USA).

    Techniques: Real-time Polymerase Chain Reaction

    Melting curve analysis of the targeted eight poxviruses using different PCR platforms. Each genotype displayed a unique melting peak. ( a ). amplification plot; ( b ). melt curve plot; ( c ). melting peaks using the QuantStudio 6, Life Technologies); ( d ). melting peaks using the CFX96, Bio-Rad; ( e ). melting peaks using LC480II, Roche; ( f ). melting peaks using the Rotor Gene Q, Qiagen); ( g ). Linearity test (10 9 to 10 2 virus copies); and ( h ). Co-infection with CMLV and camel PCPV (blue colour two melting peaks, 72.80 °C and 81.20 °C for CMLV and camel PCPV respectively).

    Journal: Scientific Reports

    Article Title: A novel HRM assay for the simultaneous detection and differentiation of eight poxviruses of medical and veterinary importance

    doi: 10.1038/srep42892

    Figure Lengend Snippet: Melting curve analysis of the targeted eight poxviruses using different PCR platforms. Each genotype displayed a unique melting peak. ( a ). amplification plot; ( b ). melt curve plot; ( c ). melting peaks using the QuantStudio 6, Life Technologies); ( d ). melting peaks using the CFX96, Bio-Rad; ( e ). melting peaks using LC480II, Roche; ( f ). melting peaks using the Rotor Gene Q, Qiagen); ( g ). Linearity test (10 9 to 10 2 virus copies); and ( h ). Co-infection with CMLV and camel PCPV (blue colour two melting peaks, 72.80 °C and 81.20 °C for CMLV and camel PCPV respectively).

    Article Snippet: Furthermore, after performing the amplification steps using the classical PCR machine (Bio-Rad C1000) and transferring the plates containing the amplified product to the CFX96™ real-time PCR system (Bio-Rad) only for melting curve analysis, we have successfully assigned each virus to the correct species.

    Techniques: Polymerase Chain Reaction, Amplification, Infection

    Expression analysis of genes identified in the papilla by LM–RNA-seq. qRT-PCR was performed using the SYBR Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.

    Journal: Plant and Cell Physiology

    Article Title: Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

    doi: 10.1093/pcp/pct133

    Figure Lengend Snippet: Expression analysis of genes identified in the papilla by LM–RNA-seq. qRT-PCR was performed using the SYBR Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.

    Article Snippet: Total RNA was reverse-transcribed using the SuperScript III First-Strand Synthesis system with oligo(dT) primer, according to the manufacturer’s instructions. qRT-PCR was performed using the SYBR Green Real-Time PCR Master Mix (Toyobo) with a Bio-Rad CFX96 Real-Time Detection System.

    Techniques: Expressing, RNA Sequencing Assay, Quantitative RT-PCR, SYBR Green Assay, Real-time Polymerase Chain Reaction

    Protein thermal shift induced by M36 binding p32. Human recombinant protein p32 was incubated with 100 µM M36 or DMSO control and protein thermal shift determined with BioRad CFX96 real-time PCR

    Journal: Journal of Translational Medicine

    Article Title: A novel small molecule inhibitor of p32 mitochondrial protein overexpressed in glioma

    doi: 10.1186/s12967-017-1312-7

    Figure Lengend Snippet: Protein thermal shift induced by M36 binding p32. Human recombinant protein p32 was incubated with 100 µM M36 or DMSO control and protein thermal shift determined with BioRad CFX96 real-time PCR

    Article Snippet: The thermal shift reaction was performed with a BioRad CFX96 real-time PCR machine, and analysis for binding induced shifts in thermal transition was performed with Precision Melt Analysis Software provided by the manufacturer (BioRad).

    Techniques: Binding Assay, Recombinant, Incubation, Real-time Polymerase Chain Reaction

    DNA, RNA and 2′-OMe protected RNA probes give excellent mismatch discrimination and fluorescence enhancement with RNA targets. Derivatives of fluorescence melting curves for RNA duplexes of ( A ) S1-TO3/ROX DNA probe, ( B ) S2-TO3/ROX RNA probe and ( C ) S3-TO3/ROX 2′-OMe RNA probe in phosphate buffer, NaH 2 PO 4 , 10 mM, 200 mM NaCl at pH 7.4 (concentration of probe = 0.5 μM; concentration of target = 0.75 μM). For controls (dark blue) no target was used. All output was monitored in the ‘ROX’ channel of the CFX96 Real-Time PCR instrument (excitation range 560–590 nm, detector range 610–650 nm).

    Journal: Nucleic Acids Research

    Article Title: Combination probes with intercalating anchors and proximal fluorophores for DNA and RNA detection

    doi: 10.1093/nar/gkw579

    Figure Lengend Snippet: DNA, RNA and 2′-OMe protected RNA probes give excellent mismatch discrimination and fluorescence enhancement with RNA targets. Derivatives of fluorescence melting curves for RNA duplexes of ( A ) S1-TO3/ROX DNA probe, ( B ) S2-TO3/ROX RNA probe and ( C ) S3-TO3/ROX 2′-OMe RNA probe in phosphate buffer, NaH 2 PO 4 , 10 mM, 200 mM NaCl at pH 7.4 (concentration of probe = 0.5 μM; concentration of target = 0.75 μM). For controls (dark blue) no target was used. All output was monitored in the ‘ROX’ channel of the CFX96 Real-Time PCR instrument (excitation range 560–590 nm, detector range 610–650 nm).

    Article Snippet: Asymmetric PCR using BioRad CFX96 real-time PCR instrument Reactions were undertaken using a BioRad CFX96 Real-Time PCR Instrument, with CFX Manager software (BioRad), monitoring in the following channels: FAM channel (excitation range 450–490 nm, detector range 510–530 nm), HEX channel (excitation range 515–535 nm, detector range 560–580 nm), ROX channel (excitation range 560–590 nm, detector range 610–650 nm) and Cy5 channel (excitation range 620–650 nm, detector range 675–690 nm).

    Techniques: Fluorescence, Concentration Assay, Real-time Polymerase Chain Reaction

    Mutation discrimination by means of base pair mismatches. Fluorescence melting curves ( A and C ) and derivatives ( B and D ) for matched and mismatched duplexes of WT probe L1-(TO3/ROX) 2 and MT probe L2-(TO3/ATTO647N) 2 . Fluorescence was monitored in the ‘ROX’ channel (for ROX, excitation range 560–590 nm, detection range = 610–650 nm) or ‘Cy5’ channel (for ATTO647N, excitation range = 620–650 nm, detection range = 675–690 nm) of the CFX96 RT-PCR instrument. KOD XL DNA polymerase buffer (pH 7.5) was used. Δ T compares the mismatched and fully matched DNA duplexes. The probe/target duplexes are shown schematically ( E ) with base pairs at the site of mutation highlighted.

    Journal: Nucleic Acids Research

    Article Title: Combination probes with intercalating anchors and proximal fluorophores for DNA and RNA detection

    doi: 10.1093/nar/gkw579

    Figure Lengend Snippet: Mutation discrimination by means of base pair mismatches. Fluorescence melting curves ( A and C ) and derivatives ( B and D ) for matched and mismatched duplexes of WT probe L1-(TO3/ROX) 2 and MT probe L2-(TO3/ATTO647N) 2 . Fluorescence was monitored in the ‘ROX’ channel (for ROX, excitation range 560–590 nm, detection range = 610–650 nm) or ‘Cy5’ channel (for ATTO647N, excitation range = 620–650 nm, detection range = 675–690 nm) of the CFX96 RT-PCR instrument. KOD XL DNA polymerase buffer (pH 7.5) was used. Δ T compares the mismatched and fully matched DNA duplexes. The probe/target duplexes are shown schematically ( E ) with base pairs at the site of mutation highlighted.

    Article Snippet: Asymmetric PCR using BioRad CFX96 real-time PCR instrument Reactions were undertaken using a BioRad CFX96 Real-Time PCR Instrument, with CFX Manager software (BioRad), monitoring in the following channels: FAM channel (excitation range 450–490 nm, detector range 510–530 nm), HEX channel (excitation range 515–535 nm, detector range 560–580 nm), ROX channel (excitation range 560–590 nm, detector range 610–650 nm) and Cy5 channel (excitation range 620–650 nm, detector range 675–690 nm).

    Techniques: Mutagenesis, Fluorescence, Reverse Transcription Polymerase Chain Reaction

    Transcript analysis of PGE2 receptors in THP-1 macrophages during S. Typhimurium and Y. enterocolitica infection. THP-1 macrophages (2.5 × 10 5 ) were infected for 2 h with (A) S. Typhimurium or (B) Y. enterocolitica at an MOI of 50:1. Total RNA was extracted using a PureLink RNA mini kit, and cDNA was generated using the iScript system (Bio-Rad). RT-PCR was performed using SYBR green on a CFX96 Real-Time System (Bio-Rad) targeting prostanoid receptors EP1, EP2, EP3, EP4, and GAPDH as a reference gene. Resulting data were then normalized to GAPDH mRNA levels, and uninfected samples served as a baseline for fold change determination using the ΔΔCt method. Resulting data is representative of three biological replicates and three technical replicates and represents the mean fold change ± SD. One-way ANOVA test with Tukey’s multiple testing correction was used to establish statistical significance. p -values were indicated as follows: ∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001; ∗∗∗∗ p ≤ 0.0001.

    Journal: Frontiers in Microbiology

    Article Title: PGE2 Augments Inflammasome Activation and M1 Polarization in Macrophages Infected With Salmonella Typhimurium and Yersinia enterocolitica

    doi: 10.3389/fmicb.2018.02447

    Figure Lengend Snippet: Transcript analysis of PGE2 receptors in THP-1 macrophages during S. Typhimurium and Y. enterocolitica infection. THP-1 macrophages (2.5 × 10 5 ) were infected for 2 h with (A) S. Typhimurium or (B) Y. enterocolitica at an MOI of 50:1. Total RNA was extracted using a PureLink RNA mini kit, and cDNA was generated using the iScript system (Bio-Rad). RT-PCR was performed using SYBR green on a CFX96 Real-Time System (Bio-Rad) targeting prostanoid receptors EP1, EP2, EP3, EP4, and GAPDH as a reference gene. Resulting data were then normalized to GAPDH mRNA levels, and uninfected samples served as a baseline for fold change determination using the ΔΔCt method. Resulting data is representative of three biological replicates and three technical replicates and represents the mean fold change ± SD. One-way ANOVA test with Tukey’s multiple testing correction was used to establish statistical significance. p -values were indicated as follows: ∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001; ∗∗∗∗ p ≤ 0.0001.

    Article Snippet: The cDNA was synthesized using a Bio-Rad iScript cDNA Synthesis Kit and expression of genes encoding EP1, EP2, EP3, and EP4 was measured as stated above on a Bio-Rad CFX96 Real-Time System.

    Techniques: Infection, Generated, Reverse Transcription Polymerase Chain Reaction, SYBR Green Assay

    Fluorescence signal intensity determined by real-time reverse transcription–polymerase chain reaction analysis, using Zaire Ebolavirus and Filovirus Screen kits. Dilutions of Ebola virus (EBOV) RNA were assayed in parallel with Zaire Ebolavirus and Filovirus Screen kits on the CFX96 instrument. At low RNA concentrations, the fluorescence signal-to-noise ratio for the Zaire Ebolavirus kit is improved, compared with that for the Filovirus Screen kit.

    Journal: The Journal of Infectious Diseases

    Article Title: Evaluation of RealStar Reverse Transcription–Polymerase Chain Reaction Kits for Filovirus Detection in the Laboratory and Field

    doi: 10.1093/infdis/jiw246

    Figure Lengend Snippet: Fluorescence signal intensity determined by real-time reverse transcription–polymerase chain reaction analysis, using Zaire Ebolavirus and Filovirus Screen kits. Dilutions of Ebola virus (EBOV) RNA were assayed in parallel with Zaire Ebolavirus and Filovirus Screen kits on the CFX96 instrument. At low RNA concentrations, the fluorescence signal-to-noise ratio for the Zaire Ebolavirus kit is improved, compared with that for the Filovirus Screen kit.

    Article Snippet: The extracted RNA of early and late samples was retested by using the RealStar kits on Rotor-Gene and CFX96.

    Techniques: Fluorescence, Reverse Transcription Polymerase Chain Reaction

    Amplification efficiency of four qPCR instruments; CFX96 (green), xxpress (blue), ABI Prism 7900 HT (red) and Rotor-Gene Q (purple).

    Journal: Experimental and Therapeutic Medicine

    Article Title: Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives

    doi: 10.3892/etm.2015.2712

    Figure Lengend Snippet: Amplification efficiency of four qPCR instruments; CFX96 (green), xxpress (blue), ABI Prism 7900 HT (red) and Rotor-Gene Q (purple).

    Article Snippet: Thermal variability was assessed in qPCR by measuring the amplification of 18S rRNA in a selection of wells covering all areas of the sample plate on ABI Prism 7900HT, Bio-Rad CFX96 System, Qiagen Rotor-Gene Q and BJS Biotechnologies xxpress instruments.

    Techniques: Amplification, Real-time Polymerase Chain Reaction

    Thermal variability upon amplification of 18s rRNA using 5 ng/µl human genomic DNA. (A) CFX96, (B) xxpress®, (C) ABI Prism 7900HT and (D) Rotor-Gene Q.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives

    doi: 10.3892/etm.2015.2712

    Figure Lengend Snippet: Thermal variability upon amplification of 18s rRNA using 5 ng/µl human genomic DNA. (A) CFX96, (B) xxpress®, (C) ABI Prism 7900HT and (D) Rotor-Gene Q.

    Article Snippet: Thermal variability was assessed in qPCR by measuring the amplification of 18S rRNA in a selection of wells covering all areas of the sample plate on ABI Prism 7900HT, Bio-Rad CFX96 System, Qiagen Rotor-Gene Q and BJS Biotechnologies xxpress instruments.

    Techniques: Amplification

    Decision tree flowchart developed using the interpretation rules for the triplex real-time PCR assay run on a Bio-Rad CFX96 system.

    Journal: PLoS ONE

    Article Title: A Multiplex Real-Time PCR Assay to Diagnose and Separate Helicoverpa armigera and H. zea (Lepidoptera: Noctuidae) in the New World

    doi: 10.1371/journal.pone.0142912

    Figure Lengend Snippet: Decision tree flowchart developed using the interpretation rules for the triplex real-time PCR assay run on a Bio-Rad CFX96 system.

    Article Snippet: Based on serial dilution data, the Cepheid system is less sensitive than the Bio-Rad CFX96.

    Techniques: Real-time Polymerase Chain Reaction

    Sample analysis workflow for suspected avian botulism cultures. Parameters evaluated during the study: test sample type (whole organ means that the whole liver or up to 25g of the liver was analyzed), enrichment culturing conditions, DNA extraction methods. The CFX96 thermocycler (Bio-Rad, Marne-la-Coquette, France) was used for Real-Time PCR. Analyses performed by ANSES are indicated in black, and performed by LABOCEA are in grey. Kit 1: QIAamp ® DNA Mini kit (Qiagen, Courtaboeuf, France), Kit2: InstaGene Matrix (Bio-Rad, Marne-la-Coquette, France), Kit3: Mericon Bacteria+ (Qiagen, Courtaboeuf, France). CII, CIII, DII, DIII, E are the primers and probe used to perform real-time PCR for the detection of type C, D, C/D, D/C and E BoNT genes [ 4 , 14 ]. L: Liver; L1 to L5: Liver N° 1 to Liver N° 5. M1 to M4: Method 1 to method 4.

    Journal: PLoS ONE

    Article Title: Development and Validation of a New Reliable Method for the Diagnosis of Avian Botulism

    doi: 10.1371/journal.pone.0169640

    Figure Lengend Snippet: Sample analysis workflow for suspected avian botulism cultures. Parameters evaluated during the study: test sample type (whole organ means that the whole liver or up to 25g of the liver was analyzed), enrichment culturing conditions, DNA extraction methods. The CFX96 thermocycler (Bio-Rad, Marne-la-Coquette, France) was used for Real-Time PCR. Analyses performed by ANSES are indicated in black, and performed by LABOCEA are in grey. Kit 1: QIAamp ® DNA Mini kit (Qiagen, Courtaboeuf, France), Kit2: InstaGene Matrix (Bio-Rad, Marne-la-Coquette, France), Kit3: Mericon Bacteria+ (Qiagen, Courtaboeuf, France). CII, CIII, DII, DIII, E are the primers and probe used to perform real-time PCR for the detection of type C, D, C/D, D/C and E BoNT genes [ 4 , 14 ]. L: Liver; L1 to L5: Liver N° 1 to Liver N° 5. M1 to M4: Method 1 to method 4.

    Article Snippet: Real-time PCR using a Bio-Rad CFX96 thermal cycler (Bio-Rad,) was used to detect C . botulinum types C, C/D, D, D/C and E. Each assay was performed in a total volume of 20 μl, containing 5 μl DNA template, 10 μl IQ supermix (Bio-Rad, Marne-la-Coquette, France) and a final concentration of 600 nM for primers and 400 nM for probes.

    Techniques: DNA Extraction, Real-time Polymerase Chain Reaction

    Expression of E1 during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: E1A ≤ 0.001; E1B-19k

    Journal: PLoS ONE

    Article Title: Temporal dynamics of adenovirus 5 gene expression in normal human cells

    doi: 10.1371/journal.pone.0211192

    Figure Lengend Snippet: Expression of E1 during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: E1A ≤ 0.001; E1B-19k

    Article Snippet: The cDNA was subsequently used either for digital quantification using the BioRad QX200 digital droplet PCR system with automatic droplet generator with ddPCR Probe Master Mix, or using SsoAdvanced Master Mix for probes for conventional qRT-PCR analysis on BioRad CFX96 for time points after 17 hours.

    Techniques: Expressing, Infection, Digital PCR, Real-time Polymerase Chain Reaction, Standard Deviation

    Expression of E2 , E3 , and E4 during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at an MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: DBP

    Journal: PLoS ONE

    Article Title: Temporal dynamics of adenovirus 5 gene expression in normal human cells

    doi: 10.1371/journal.pone.0211192

    Figure Lengend Snippet: Expression of E2 , E3 , and E4 during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at an MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: DBP

    Article Snippet: The cDNA was subsequently used either for digital quantification using the BioRad QX200 digital droplet PCR system with automatic droplet generator with ddPCR Probe Master Mix, or using SsoAdvanced Master Mix for probes for conventional qRT-PCR analysis on BioRad CFX96 for time points after 17 hours.

    Techniques: Expressing, Infection, Digital PCR, Real-time Polymerase Chain Reaction, Standard Deviation

    Expression of VAII RNA during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the p ≤ 0.0027. All time-points between 17 and 36 hours were statistically significant.

    Journal: PLoS ONE

    Article Title: Temporal dynamics of adenovirus 5 gene expression in normal human cells

    doi: 10.1371/journal.pone.0211192

    Figure Lengend Snippet: Expression of VAII RNA during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the p ≤ 0.0027. All time-points between 17 and 36 hours were statistically significant.

    Article Snippet: The cDNA was subsequently used either for digital quantification using the BioRad QX200 digital droplet PCR system with automatic droplet generator with ddPCR Probe Master Mix, or using SsoAdvanced Master Mix for probes for conventional qRT-PCR analysis on BioRad CFX96 for time points after 17 hours.

    Techniques: Expressing, Infection, Digital PCR, Real-time Polymerase Chain Reaction, Standard Deviation

    Expression of viral late genes during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: 52k EP ≤ 0.001; pIIIa ≤ 0.0006; Penton base ≤ 0.0001; Hexon ≤ 0.0002; 100k HAP ≤ 0.0007; Fiber ≤ 0.0004. All time-points between 17 and 36 hours were statistically significant.

    Journal: PLoS ONE

    Article Title: Temporal dynamics of adenovirus 5 gene expression in normal human cells

    doi: 10.1371/journal.pone.0211192

    Figure Lengend Snippet: Expression of viral late genes during the first 36 hours after infection with HAdV5. Arrested IMR-90 cells were infected with dl 309 at a MOI of 50 and total RNA was extracted at the indicated time points using Nucleozol reagent, treated with DNase I and reverse-transcribed using VILO. Transcript levels of the indicated viral mRNAs were quantified using the BioRad QX200 droplet digital PCR system for the first 16 hours, and using BioRad CFX96 real-time PCR instrument for 17 to 36 hours, and plotted as percentage of GAPDH expression. Right column shows droplet distribution at the time when transcripts increase above background levels. Error bars represent standard deviation of biological replicates, n = 3. Bar with asterisk represents changes above mock level that are statistically significant with the following p values: 52k EP ≤ 0.001; pIIIa ≤ 0.0006; Penton base ≤ 0.0001; Hexon ≤ 0.0002; 100k HAP ≤ 0.0007; Fiber ≤ 0.0004. All time-points between 17 and 36 hours were statistically significant.

    Article Snippet: The cDNA was subsequently used either for digital quantification using the BioRad QX200 digital droplet PCR system with automatic droplet generator with ddPCR Probe Master Mix, or using SsoAdvanced Master Mix for probes for conventional qRT-PCR analysis on BioRad CFX96 for time points after 17 hours.

    Techniques: Expressing, Infection, Digital PCR, Real-time Polymerase Chain Reaction, Standard Deviation

    Decision tree flowchart developed using the interpretation rules for the triplex real-time PCR assay run on a Bio-Rad CFX96 system.

    Journal: PLoS ONE

    Article Title: A Multiplex Real-Time PCR Assay to Diagnose and Separate Helicoverpa armigera and H. zea (Lepidoptera: Noctuidae) in the New World

    doi: 10.1371/journal.pone.0142912

    Figure Lengend Snippet: Decision tree flowchart developed using the interpretation rules for the triplex real-time PCR assay run on a Bio-Rad CFX96 system.

    Article Snippet: Development of interpretation rules The interpretation rules developed here are based on the Cq values obtained from the 452 samples tested with the real-time PCR assay on a Bio-Rad CFX96 Touch Real-time PCR Detection System.

    Techniques: Real-time Polymerase Chain Reaction