cft Search Results


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  • 88
    Millipore methylene blue
    Methylene Blue, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 2586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    methylene blue - by Bioz Stars, 2020-08
    88/100 stars
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    cft  (Ikaria)
    92
    Ikaria cft
    Cft, supplied by Ikaria, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cft/product/Ikaria
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    cft  (Organix)
    91
    Organix cft
    Cft, supplied by Organix, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cft - by Bioz Stars, 2020-08
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    92
    Carl Zeiss cft
    Cft, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Charles River Laboratories cft
    Cryofluorescence tomography <t>(CFT)</t> of <t>ASO</t> permeation into brain tissue. ( A ) Cy7-labeled Gabra1 ASOs (700 μg) were dosed IT, brains removed for CFT at various times after dosing, and photographed under white and fluorescent light after each 25-μm block-face section. ( B ) Trans-pia movement of Gabra1 Cy7-ASOs (×10 magnification). ( C ) Vascular association and cell uptake of Gabra1 Cy7-ASOs (×20 magnification). ( D ) Peri-arterial association of Cy7-ASO in 3D reconstruction of CFT data. Arrowhead points to basilar and middle cerebral arteries. Deeper vessels seen include the interhemispheric anterior cerebral artery.
    Cft, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cft - by Bioz Stars, 2020-08
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    91
    Glaxo Smith cft
    Protease activities of supernatants from P. aeruginosa cultures grown either without antibiotics (black bar) or in the presence of 2 μg/ml <t>AZM</t> (bar with horizontal lines), 8 μg/ml AZM (bar with vertical lines), 0.25 μg/ml <t>CFT</t> (dark
    Cft, supplied by Glaxo Smith, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore cft
    Evidence for involvement of Val152 3.46 in binding of <t>BZT</t> and JHW007. (A) [ 3 <t>H]CFT/CFT</t> competition binding on wild type (WT) DAT (black circles) or V152I (blue square). (B, C) Inhibition of [ 3 H]CFT binding by BZT (B) or JHW007 (C) to wild type DAT (black
    Cft, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    National Veterinary Services Laboratories cft
    Evidence for involvement of Val152 3.46 in binding of <t>BZT</t> and JHW007. (A) [ 3 <t>H]CFT/CFT</t> competition binding on wild type (WT) DAT (black circles) or V152I (blue square). (B, C) Inhibition of [ 3 H]CFT binding by BZT (B) or JHW007 (C) to wild type DAT (black
    Cft, supplied by National Veterinary Services Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    heidelberg engineering cft
    Evidence for involvement of Val152 3.46 in binding of <t>BZT</t> and JHW007. (A) [ 3 <t>H]CFT/CFT</t> competition binding on wild type (WT) DAT (black circles) or V152I (blue square). (B, C) Inhibition of [ 3 H]CFT binding by BZT (B) or JHW007 (C) to wild type DAT (black
    Cft, supplied by heidelberg engineering, used in various techniques. Bioz Stars score: 91/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cft  (IDEXX)
    92
    IDEXX cft
    Evolution of <t>CFT</t> titers (ICFTU/mL) and <t>RBT</t> scores in Capra hircus . A Vaccinated goats, necropsied 45 days post-vaccination, and B vaccinated and control goats both necropsied 90 days post-vaccination. Titers and scores were obtained on days 0, 20, 45, 68 and 90 after conjunctival vaccination on day 0 with B. melitensis Rev.1 strain, in both males (M) and females (F) (squares continuous lines for CFT titers and respectively diamonds in dotted lines for RBT score).
    Cft, supplied by IDEXX, used in various techniques. Bioz Stars score: 92/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Virion\Serion cft
    Evolution of <t>CFT</t> titers (ICFTU/mL) and <t>RBT</t> scores in Capra hircus . A Vaccinated goats, necropsied 45 days post-vaccination, and B vaccinated and control goats both necropsied 90 days post-vaccination. Titers and scores were obtained on days 0, 20, 45, 68 and 90 after conjunctival vaccination on day 0 with B. melitensis Rev.1 strain, in both males (M) and females (F) (squares continuous lines for CFT titers and respectively diamonds in dotted lines for RBT score).
    Cft, supplied by Virion\Serion, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cft  (Zoetis)
    92
    Zoetis cft
    Evolution of <t>CFT</t> titers (ICFTU/mL) and <t>RBT</t> scores in Capra hircus . A Vaccinated goats, necropsied 45 days post-vaccination, and B vaccinated and control goats both necropsied 90 days post-vaccination. Titers and scores were obtained on days 0, 20, 45, 68 and 90 after conjunctival vaccination on day 0 with B. melitensis Rev.1 strain, in both males (M) and females (F) (squares continuous lines for CFT titers and respectively diamonds in dotted lines for RBT score).
    Cft, supplied by Zoetis, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher cft
    Evolution of <t>CFT</t> titers (ICFTU/mL) and <t>RBT</t> scores in Capra hircus . A Vaccinated goats, necropsied 45 days post-vaccination, and B vaccinated and control goats both necropsied 90 days post-vaccination. Titers and scores were obtained on days 0, 20, 45, 68 and 90 after conjunctival vaccination on day 0 with B. melitensis Rev.1 strain, in both males (M) and females (F) (squares continuous lines for CFT titers and respectively diamonds in dotted lines for RBT score).
    Cft, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad cft ceramic fluoroapatite
    Evolution of <t>CFT</t> titers (ICFTU/mL) and <t>RBT</t> scores in Capra hircus . A Vaccinated goats, necropsied 45 days post-vaccination, and B vaccinated and control goats both necropsied 90 days post-vaccination. Titers and scores were obtained on days 0, 20, 45, 68 and 90 after conjunctival vaccination on day 0 with B. melitensis Rev.1 strain, in both males (M) and females (F) (squares continuous lines for CFT titers and respectively diamonds in dotted lines for RBT score).
    Cft Ceramic Fluoroapatite, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore β 11 c cft
    Effects of scopolamine on the time–activity curves of [β- 11 <t>C]CFT</t> in the brain ( A ) and in the arterial plasma ( B ). Saline or scopolamine (10 or 100 μg/kg) was administered 30 min before [β- 11 C]CFT injection. PET scan was
    β 11 C Cft, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    PerkinElmer h cft
    Evidence for involvement of Asn157 3.51 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by <t>CFT</t> (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on <t>COS7</t> cells transiently
    H Cft, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Virion\Serion cft buffer
    Evidence for involvement of Asn157 3.51 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by <t>CFT</t> (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on <t>COS7</t> cells transiently
    Cft Buffer, supplied by Virion\Serion, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Technology Development Co cft 1
    Evidence for involvement of Asn157 3.51 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by <t>CFT</t> (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on <t>COS7</t> cells transiently
    Cft 1, supplied by Technology Development Co, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cft 1 - by Bioz Stars, 2020-08
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    93
    Shimadzu Corporation cft 100ex capillary rheometer
    Evidence for involvement of Asn157 3.51 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by <t>CFT</t> (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on <t>COS7</t> cells transiently
    Cft 100ex Capillary Rheometer, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Virion\Serion complement fixation test cft
    Evidence for involvement of Asn157 3.51 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by <t>CFT</t> (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on <t>COS7</t> cells transiently
    Complement Fixation Test Cft, supplied by Virion\Serion, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Illumina Inc cft strains
    BRIG plot of C . fetus . BLASTN-based genomic comparison of all <t>Cft</t> strains, including Cft strain 03-427 (reference genome), <t>Cff</t> strain 82-40, and Cfv strain 97/608. Cft strains isolated from humans are shown in blue; Cft strains isolated from reptiles are shown in green, with predicted Cft sapA strains shown in dark green and predicted Cft sapB and sapAB strains shown in light green; Cff is shown in yellow; and Cfv is shown in red. Characteristic genomic features of C . fetus and Cft in particular have been highlighted. Features containing only hypothetical proteins are not indicated.
    Cft Strains, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    PerkinElmer 3 h cft
    AIM-100 non-competitively inhibits <t>CFT</t> binding. ( A and B ) PAE/YFP-DAT cells were incubated with the range of <t>[3-H]CFT</t> concentrations in F12 medium with DMSO (Veh) or 20 µM AIM-100 at 20°C for 30 min as described in ‘Methods’. ( A ) Examples of the concentration-dependence plots of [3-H]CFT binding. ( B ) Mean values of K D and Bmax (±S.E.M.) were determined in five independent experiments exemplified in ( A ). P values were calculated for ‘AIM-100’ versus ‘vehicle’ using paired two-tailed t-test. ( C ) PAE/YFP-DAT cells were incubated with vehicle (DMSO) or 20 µM AIM-100 for 2 hr at 4°C, 20°C or 37°C, lysed, and YFP-DAT was detected by blotting with the GFP antibody. M , monomers; O , oligomers.
    3 H Cft, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Combino Pharm ceftazidime cft
    AIM-100 non-competitively inhibits <t>CFT</t> binding. ( A and B ) PAE/YFP-DAT cells were incubated with the range of <t>[3-H]CFT</t> concentrations in F12 medium with DMSO (Veh) or 20 µM AIM-100 at 20°C for 30 min as described in ‘Methods’. ( A ) Examples of the concentration-dependence plots of [3-H]CFT binding. ( B ) Mean values of K D and Bmax (±S.E.M.) were determined in five independent experiments exemplified in ( A ). P values were calculated for ‘AIM-100’ versus ‘vehicle’ using paired two-tailed t-test. ( C ) PAE/YFP-DAT cells were incubated with vehicle (DMSO) or 20 µM AIM-100 for 2 hr at 4°C, 20°C or 37°C, lysed, and YFP-DAT was detected by blotting with the GFP antibody. M , monomers; O , oligomers.
    Ceftazidime Cft, supplied by Combino Pharm, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Carl Zeiss axiovert 40 cft visible fluorescence microscope
    AIM-100 non-competitively inhibits <t>CFT</t> binding. ( A and B ) PAE/YFP-DAT cells were incubated with the range of <t>[3-H]CFT</t> concentrations in F12 medium with DMSO (Veh) or 20 µM AIM-100 at 20°C for 30 min as described in ‘Methods’. ( A ) Examples of the concentration-dependence plots of [3-H]CFT binding. ( B ) Mean values of K D and Bmax (±S.E.M.) were determined in five independent experiments exemplified in ( A ). P values were calculated for ‘AIM-100’ versus ‘vehicle’ using paired two-tailed t-test. ( C ) PAE/YFP-DAT cells were incubated with vehicle (DMSO) or 20 µM AIM-100 for 2 hr at 4°C, 20°C or 37°C, lysed, and YFP-DAT was detected by blotting with the GFP antibody. M , monomers; O , oligomers.
    Axiovert 40 Cft Visible Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cryofluorescence tomography (CFT) of ASO permeation into brain tissue. ( A ) Cy7-labeled Gabra1 ASOs (700 μg) were dosed IT, brains removed for CFT at various times after dosing, and photographed under white and fluorescent light after each 25-μm block-face section. ( B ) Trans-pia movement of Gabra1 Cy7-ASOs (×10 magnification). ( C ) Vascular association and cell uptake of Gabra1 Cy7-ASOs (×20 magnification). ( D ) Peri-arterial association of Cy7-ASO in 3D reconstruction of CFT data. Arrowhead points to basilar and middle cerebral arteries. Deeper vessels seen include the interhemispheric anterior cerebral artery.

    Journal: JCI Insight

    Article Title: Brain pharmacology of intrathecal antisense oligonucleotides revealed through multimodal imaging

    doi: 10.1172/jci.insight.129240

    Figure Lengend Snippet: Cryofluorescence tomography (CFT) of ASO permeation into brain tissue. ( A ) Cy7-labeled Gabra1 ASOs (700 μg) were dosed IT, brains removed for CFT at various times after dosing, and photographed under white and fluorescent light after each 25-μm block-face section. ( B ) Trans-pia movement of Gabra1 Cy7-ASOs (×10 magnification). ( C ) Vascular association and cell uptake of Gabra1 Cy7-ASOs (×20 magnification). ( D ) Peri-arterial association of Cy7-ASO in 3D reconstruction of CFT data. Arrowhead points to basilar and middle cerebral arteries. Deeper vessels seen include the interhemispheric anterior cerebral artery.

    Article Snippet: Animals used in the 125 I- Malat1 ASO imaging, CFT, and Gabra1 18 F-flumazenil imaging studies were obtained from Charles River Laboratories.

    Techniques: Allele-specific Oligonucleotide, Labeling, Blocking Assay

    2D and 3D CFT display of IT-dosed Gabra1 Cy7-ASO brain penetration over time. Left side shows sagittal, coronal, and horizontal virtual sections through the 3D-reconstructed CFT fluorescence images of animals euthanized 1, 2, 4, 6, 8, and 12 hours after IT dosing of Cy7-labeled Gabra1 ASOs (700 μg). Right side shows the surface images of the ventral, dorsal, and lateral aspects of the 3D-reconstructed CFT fluorescence images at the same time points. On the far right are images of the forebrain surface of the 3D-reconstructed CFT fluorescence images showing the fluorescently labeled ASO association with the left middle cerebral artery (MCA) at the different time points. For all images the fluorescence intensity was kept constant to evaluate the relative distribution of the ASO in the brain.

    Journal: JCI Insight

    Article Title: Brain pharmacology of intrathecal antisense oligonucleotides revealed through multimodal imaging

    doi: 10.1172/jci.insight.129240

    Figure Lengend Snippet: 2D and 3D CFT display of IT-dosed Gabra1 Cy7-ASO brain penetration over time. Left side shows sagittal, coronal, and horizontal virtual sections through the 3D-reconstructed CFT fluorescence images of animals euthanized 1, 2, 4, 6, 8, and 12 hours after IT dosing of Cy7-labeled Gabra1 ASOs (700 μg). Right side shows the surface images of the ventral, dorsal, and lateral aspects of the 3D-reconstructed CFT fluorescence images at the same time points. On the far right are images of the forebrain surface of the 3D-reconstructed CFT fluorescence images showing the fluorescently labeled ASO association with the left middle cerebral artery (MCA) at the different time points. For all images the fluorescence intensity was kept constant to evaluate the relative distribution of the ASO in the brain.

    Article Snippet: Animals used in the 125 I- Malat1 ASO imaging, CFT, and Gabra1 18 F-flumazenil imaging studies were obtained from Charles River Laboratories.

    Techniques: Allele-specific Oligonucleotide, Fluorescence, Labeling

    Protease activities of supernatants from P. aeruginosa cultures grown either without antibiotics (black bar) or in the presence of 2 μg/ml AZM (bar with horizontal lines), 8 μg/ml AZM (bar with vertical lines), 0.25 μg/ml CFT (dark

    Journal:

    Article Title: Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa ▿

    doi: 10.1128/AAC.01230-07

    Figure Lengend Snippet: Protease activities of supernatants from P. aeruginosa cultures grown either without antibiotics (black bar) or in the presence of 2 μg/ml AZM (bar with horizontal lines), 8 μg/ml AZM (bar with vertical lines), 0.25 μg/ml CFT (dark

    Article Snippet: When the OD600 was 0.3, AZM (8 and 2 μg/ml; Zithromax; Pfizer), CPR (0.04 μg/ml; Fluka), or CFT (0.25 μg/ml; Fortum; GlaxoSmithKline) was added.

    Techniques:

    AZM, CFT, and CPR affect expression of QS-regulated genes.

    Journal:

    Article Title: Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa ▿

    doi: 10.1128/AAC.01230-07

    Figure Lengend Snippet: AZM, CFT, and CPR affect expression of QS-regulated genes.

    Article Snippet: When the OD600 was 0.3, AZM (8 and 2 μg/ml; Zithromax; Pfizer), CPR (0.04 μg/ml; Fluka), or CFT (0.25 μg/ml; Fortum; GlaxoSmithKline) was added.

    Techniques: Expressing

    LasR, RhlR, and LasR-RhlR specificities of AZM (white bars), CFT (black bars), CPR (light gray bars), and C30 (dark gray bars). See the text for the definitions of groups (Gr.) A, B, C, and D.

    Journal:

    Article Title: Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa ▿

    doi: 10.1128/AAC.01230-07

    Figure Lengend Snippet: LasR, RhlR, and LasR-RhlR specificities of AZM (white bars), CFT (black bars), CPR (light gray bars), and C30 (dark gray bars). See the text for the definitions of groups (Gr.) A, B, C, and D.

    Article Snippet: When the OD600 was 0.3, AZM (8 and 2 μg/ml; Zithromax; Pfizer), CPR (0.04 μg/ml; Fluka), or CFT (0.25 μg/ml; Fortum; GlaxoSmithKline) was added.

    Techniques:

    DNA microarray analysis of gene expression in the presence of AZM, CFT, and CPR.

    Journal:

    Article Title: Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa ▿

    doi: 10.1128/AAC.01230-07

    Figure Lengend Snippet: DNA microarray analysis of gene expression in the presence of AZM, CFT, and CPR.

    Article Snippet: When the OD600 was 0.3, AZM (8 and 2 μg/ml; Zithromax; Pfizer), CPR (0.04 μg/ml; Fluka), or CFT (0.25 μg/ml; Fortum; GlaxoSmithKline) was added.

    Techniques: Microarray, Expressing

    Docking of AZM, CFT, and CPR against the LBD of the LasR protein.

    Journal:

    Article Title: Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa ▿

    doi: 10.1128/AAC.01230-07

    Figure Lengend Snippet: Docking of AZM, CFT, and CPR against the LBD of the LasR protein.

    Article Snippet: When the OD600 was 0.3, AZM (8 and 2 μg/ml; Zithromax; Pfizer), CPR (0.04 μg/ml; Fluka), or CFT (0.25 μg/ml; Fortum; GlaxoSmithKline) was added.

    Techniques:

    Structures of the three antibiotics investigated for potential quorum sensing inhibitory activities. (A) AZM; (B) CFT; (C) CPR.

    Journal:

    Article Title: Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa ▿

    doi: 10.1128/AAC.01230-07

    Figure Lengend Snippet: Structures of the three antibiotics investigated for potential quorum sensing inhibitory activities. (A) AZM; (B) CFT; (C) CPR.

    Article Snippet: When the OD600 was 0.3, AZM (8 and 2 μg/ml; Zithromax; Pfizer), CPR (0.04 μg/ml; Fluka), or CFT (0.25 μg/ml; Fortum; GlaxoSmithKline) was added.

    Techniques:

    Hemolysis of autoclaved supernatants from P. aeruginosa PAO1 cultures grown for 20 h. PAO1 was grown without antibiotics (a) and in the presence of 2 μg/ml AZM (b), 8 μg/ml AZM (c), 0.25 μg/ml CFT (d), or 0.04 μg/ml CPR

    Journal:

    Article Title: Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa ▿

    doi: 10.1128/AAC.01230-07

    Figure Lengend Snippet: Hemolysis of autoclaved supernatants from P. aeruginosa PAO1 cultures grown for 20 h. PAO1 was grown without antibiotics (a) and in the presence of 2 μg/ml AZM (b), 8 μg/ml AZM (c), 0.25 μg/ml CFT (d), or 0.04 μg/ml CPR

    Article Snippet: When the OD600 was 0.3, AZM (8 and 2 μg/ml; Zithromax; Pfizer), CPR (0.04 μg/ml; Fluka), or CFT (0.25 μg/ml; Fortum; GlaxoSmithKline) was added.

    Techniques:

    Evidence for involvement of Val152 3.46 in binding of BZT and JHW007. (A) [ 3 H]CFT/CFT competition binding on wild type (WT) DAT (black circles) or V152I (blue square). (B, C) Inhibition of [ 3 H]CFT binding by BZT (B) or JHW007 (C) to wild type DAT (black

    Journal: Neuropharmacology

    Article Title: The binding sites for benztropines and dopamine in the dopamine transporter overlap

    doi: 10.1016/j.neuropharm.2010.08.021

    Figure Lengend Snippet: Evidence for involvement of Val152 3.46 in binding of BZT and JHW007. (A) [ 3 H]CFT/CFT competition binding on wild type (WT) DAT (black circles) or V152I (blue square). (B, C) Inhibition of [ 3 H]CFT binding by BZT (B) or JHW007 (C) to wild type DAT (black

    Article Snippet: Cocaine HCl (Sigma-Aldrich, St. Louis, MO); CFT (Sigma-Aldrich); and BZT (Sigma-Aldrich) were obtained from the designated sources.

    Techniques: Binding Assay, Inhibition

    Evidence for involvement of Asn157 3.51 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by CFT (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on COS7 cells transiently

    Journal: Neuropharmacology

    Article Title: The binding sites for benztropines and dopamine in the dopamine transporter overlap

    doi: 10.1016/j.neuropharm.2010.08.021

    Figure Lengend Snippet: Evidence for involvement of Asn157 3.51 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by CFT (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on COS7 cells transiently

    Article Snippet: Cocaine HCl (Sigma-Aldrich, St. Louis, MO); CFT (Sigma-Aldrich); and BZT (Sigma-Aldrich) were obtained from the designated sources.

    Techniques: Binding Assay, Inhibition

    Molecular models of DAT/ligand complexes. The aligned structures of JHW007 and BZT in A illustrate their large common moiety. The docking poses of CFT (green), BZT (red) and JHW007 (blue) in DAT are superimposed in B based on the S1 binding site residues.

    Journal: Neuropharmacology

    Article Title: The binding sites for benztropines and dopamine in the dopamine transporter overlap

    doi: 10.1016/j.neuropharm.2010.08.021

    Figure Lengend Snippet: Molecular models of DAT/ligand complexes. The aligned structures of JHW007 and BZT in A illustrate their large common moiety. The docking poses of CFT (green), BZT (red) and JHW007 (blue) in DAT are superimposed in B based on the S1 binding site residues.

    Article Snippet: Cocaine HCl (Sigma-Aldrich, St. Louis, MO); CFT (Sigma-Aldrich); and BZT (Sigma-Aldrich) were obtained from the designated sources.

    Techniques: Binding Assay

    Evidence for involvement of Ala479 10.51 and Ala480 10.52 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by CFT (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on COS7

    Journal: Neuropharmacology

    Article Title: The binding sites for benztropines and dopamine in the dopamine transporter overlap

    doi: 10.1016/j.neuropharm.2010.08.021

    Figure Lengend Snippet: Evidence for involvement of Ala479 10.51 and Ala480 10.52 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by CFT (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on COS7

    Article Snippet: Cocaine HCl (Sigma-Aldrich, St. Louis, MO); CFT (Sigma-Aldrich); and BZT (Sigma-Aldrich) were obtained from the designated sources.

    Techniques: Binding Assay, Inhibition

    Evolution of CFT titers (ICFTU/mL) and RBT scores in Capra hircus . A Vaccinated goats, necropsied 45 days post-vaccination, and B vaccinated and control goats both necropsied 90 days post-vaccination. Titers and scores were obtained on days 0, 20, 45, 68 and 90 after conjunctival vaccination on day 0 with B. melitensis Rev.1 strain, in both males (M) and females (F) (squares continuous lines for CFT titers and respectively diamonds in dotted lines for RBT score).

    Journal: Veterinary Research

    Article Title: Brucella melitensis Rev.1 vaccination generates a higher shedding risk of the vaccine strain in Alpine ibex (Capra ibex) compared to the domestic goat (Capra hircus)

    doi: 10.1186/s13567-019-0717-0

    Figure Lengend Snippet: Evolution of CFT titers (ICFTU/mL) and RBT scores in Capra hircus . A Vaccinated goats, necropsied 45 days post-vaccination, and B vaccinated and control goats both necropsied 90 days post-vaccination. Titers and scores were obtained on days 0, 20, 45, 68 and 90 after conjunctival vaccination on day 0 with B. melitensis Rev.1 strain, in both males (M) and females (F) (squares continuous lines for CFT titers and respectively diamonds in dotted lines for RBT score).

    Article Snippet: Sera were examined for the presence of smooth Brucella antibodies using RBT (Pourquier® Rose Bengal Ag, IDEXX) and CFT (Pourquier® CFT Brucellosis Ag, IDEXX) antigens.

    Techniques:

    Evolution of CFT titers (ICFTU/mL) and RBT scores in Capra ibex . A Vaccinated ibexes and control ibex (identified 2349) necropsied 45 days post-vaccination, and B vaccinated and control ibexes (identified 1926 and 1895) necropsied 90 days post-vaccination. Titers and scores were obtained on days 0, 20, 45, 68 and 90 after conjunctival vaccination on day 0 with Brucella melitensis Rev.1 strain, in both males (M) and females (F) (squares continuous lines for CFT titers and respectively diamonds in dotted lines for RBT score).

    Journal: Veterinary Research

    Article Title: Brucella melitensis Rev.1 vaccination generates a higher shedding risk of the vaccine strain in Alpine ibex (Capra ibex) compared to the domestic goat (Capra hircus)

    doi: 10.1186/s13567-019-0717-0

    Figure Lengend Snippet: Evolution of CFT titers (ICFTU/mL) and RBT scores in Capra ibex . A Vaccinated ibexes and control ibex (identified 2349) necropsied 45 days post-vaccination, and B vaccinated and control ibexes (identified 1926 and 1895) necropsied 90 days post-vaccination. Titers and scores were obtained on days 0, 20, 45, 68 and 90 after conjunctival vaccination on day 0 with Brucella melitensis Rev.1 strain, in both males (M) and females (F) (squares continuous lines for CFT titers and respectively diamonds in dotted lines for RBT score).

    Article Snippet: Sera were examined for the presence of smooth Brucella antibodies using RBT (Pourquier® Rose Bengal Ag, IDEXX) and CFT (Pourquier® CFT Brucellosis Ag, IDEXX) antigens.

    Techniques:

    Effects of scopolamine on the time–activity curves of [β- 11 C]CFT in the brain ( A ) and in the arterial plasma ( B ). Saline or scopolamine (10 or 100 μg/kg) was administered 30 min before [β- 11 C]CFT injection. PET scan was

    Journal: The Journal of Neuroscience

    Article Title: Cholinergic Neuronal Modulation Alters Dopamine D2Receptor Availability In Vivo by Regulating Receptor Affinity Induced by Facilitated Synaptic Dopamine Turnover: Positron Emission Tomography Studies with Microdialysis in the Conscious Monkey Brain

    doi: 10.1523/JNEUROSCI.20-18-07067.2000

    Figure Lengend Snippet: Effects of scopolamine on the time–activity curves of [β- 11 C]CFT in the brain ( A ) and in the arterial plasma ( B ). Saline or scopolamine (10 or 100 μg/kg) was administered 30 min before [β- 11 C]CFT injection. PET scan was

    Article Snippet: Precursors for labeling of [11 C]raclopride and [β-11 C]CFT were purchased from Research Biochemicals (Natick, MA).

    Techniques: Activity Assay, Injection, Positron Emission Tomography

    Effects of scopolamine on dopamine synthesis rate, D 2 receptor binding, and transporter availability, as measured with l -[β- 11 C]DOPA ( A ), [ 11 C]raclopride ( B ), and [β- 11 C]CFT ( C ), respectively. A , The time course of changes in radioactivity

    Journal: The Journal of Neuroscience

    Article Title: Cholinergic Neuronal Modulation Alters Dopamine D2Receptor Availability In Vivo by Regulating Receptor Affinity Induced by Facilitated Synaptic Dopamine Turnover: Positron Emission Tomography Studies with Microdialysis in the Conscious Monkey Brain

    doi: 10.1523/JNEUROSCI.20-18-07067.2000

    Figure Lengend Snippet: Effects of scopolamine on dopamine synthesis rate, D 2 receptor binding, and transporter availability, as measured with l -[β- 11 C]DOPA ( A ), [ 11 C]raclopride ( B ), and [β- 11 C]CFT ( C ), respectively. A , The time course of changes in radioactivity

    Article Snippet: Precursors for labeling of [11 C]raclopride and [β-11 C]CFT were purchased from Research Biochemicals (Natick, MA).

    Techniques: Binding Assay, Radioactivity

    Evidence for involvement of Asn157 3.51 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by CFT (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on COS7 cells transiently

    Journal: Neuropharmacology

    Article Title: The binding sites for benztropines and dopamine in the dopamine transporter overlap

    doi: 10.1016/j.neuropharm.2010.08.021

    Figure Lengend Snippet: Evidence for involvement of Asn157 3.51 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by CFT (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on COS7 cells transiently

    Article Snippet: Binding assays were carried out on intact COS7 cells as described ( ) using 2–4 nM [3 H]CFT (WIN 35,428 from PerkinElmer) (87 Ci/mmol) as radioligand.

    Techniques: Binding Assay, Inhibition

    Evidence for involvement of Ala479 10.51 and Ala480 10.52 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by CFT (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on COS7

    Journal: Neuropharmacology

    Article Title: The binding sites for benztropines and dopamine in the dopamine transporter overlap

    doi: 10.1016/j.neuropharm.2010.08.021

    Figure Lengend Snippet: Evidence for involvement of Ala479 10.51 and Ala480 10.52 in binding of BZT and JHW007. (A, B, C) Inhibition of [ 3 H]dopamine uptake by CFT (A), BZT (B) or JHW007 (C). The uptake experiments were performed using indicated concentrations of inhibitor on COS7

    Article Snippet: Binding assays were carried out on intact COS7 cells as described ( ) using 2–4 nM [3 H]CFT (WIN 35,428 from PerkinElmer) (87 Ci/mmol) as radioligand.

    Techniques: Binding Assay, Inhibition

    BRIG plot of C . fetus . BLASTN-based genomic comparison of all Cft strains, including Cft strain 03-427 (reference genome), Cff strain 82-40, and Cfv strain 97/608. Cft strains isolated from humans are shown in blue; Cft strains isolated from reptiles are shown in green, with predicted Cft sapA strains shown in dark green and predicted Cft sapB and sapAB strains shown in light green; Cff is shown in yellow; and Cfv is shown in red. Characteristic genomic features of C . fetus and Cft in particular have been highlighted. Features containing only hypothetical proteins are not indicated.

    Journal: Genome Biology and Evolution

    Article Title: Comparative Genomics of Campylobacter fetus from Reptiles and Mammals Reveals Divergent Evolution in Host-Associated Lineages

    doi: 10.1093/gbe/evw146

    Figure Lengend Snippet: BRIG plot of C . fetus . BLASTN-based genomic comparison of all Cft strains, including Cft strain 03-427 (reference genome), Cff strain 82-40, and Cfv strain 97/608. Cft strains isolated from humans are shown in blue; Cft strains isolated from reptiles are shown in green, with predicted Cft sapA strains shown in dark green and predicted Cft sapB and sapAB strains shown in light green; Cff is shown in yellow; and Cfv is shown in red. Characteristic genomic features of C . fetus and Cft in particular have been highlighted. Features containing only hypothetical proteins are not indicated.

    Article Snippet: The completed genomes of Cft strains 03-427 and SP3, Cff strain 82-40, and Cfv strain 97/608 were used as reference genomes.

    Techniques: Isolation

    AIM-100 non-competitively inhibits CFT binding. ( A and B ) PAE/YFP-DAT cells were incubated with the range of [3-H]CFT concentrations in F12 medium with DMSO (Veh) or 20 µM AIM-100 at 20°C for 30 min as described in ‘Methods’. ( A ) Examples of the concentration-dependence plots of [3-H]CFT binding. ( B ) Mean values of K D and Bmax (±S.E.M.) were determined in five independent experiments exemplified in ( A ). P values were calculated for ‘AIM-100’ versus ‘vehicle’ using paired two-tailed t-test. ( C ) PAE/YFP-DAT cells were incubated with vehicle (DMSO) or 20 µM AIM-100 for 2 hr at 4°C, 20°C or 37°C, lysed, and YFP-DAT was detected by blotting with the GFP antibody. M , monomers; O , oligomers.

    Journal: eLife

    Article Title: Small molecule induced oligomerization, clustering and clathrin-independent endocytosis of the dopamine transporter

    doi: 10.7554/eLife.32293

    Figure Lengend Snippet: AIM-100 non-competitively inhibits CFT binding. ( A and B ) PAE/YFP-DAT cells were incubated with the range of [3-H]CFT concentrations in F12 medium with DMSO (Veh) or 20 µM AIM-100 at 20°C for 30 min as described in ‘Methods’. ( A ) Examples of the concentration-dependence plots of [3-H]CFT binding. ( B ) Mean values of K D and Bmax (±S.E.M.) were determined in five independent experiments exemplified in ( A ). P values were calculated for ‘AIM-100’ versus ‘vehicle’ using paired two-tailed t-test. ( C ) PAE/YFP-DAT cells were incubated with vehicle (DMSO) or 20 µM AIM-100 for 2 hr at 4°C, 20°C or 37°C, lysed, and YFP-DAT was detected by blotting with the GFP antibody. M , monomers; O , oligomers.

    Article Snippet: [3-H]CFT (82.6 Ci/mmol) was from PerkinElmers.

    Techniques: Binding Assay, Incubation, Concentration Assay, Two Tailed Test