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  • 92
    Thermo Fisher polycarbonate centrifuge tubes
    Polycarbonate Centrifuge Tubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polycarbonate centrifuge tubes/product/Thermo Fisher
    Average 92 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    polycarbonate centrifuge tubes - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    LifeSensors tandem ubiquitin binding entities tubes
    Validation of <t>anti-ubiquitin</t> antibodies. VU101 in the presence and absence of 0.5% glutaraldehyde pre-treatment, U5379, AP1228a, or P4G7-H11 were used to detect ubiquitin and ubiquitinated proteins. A) Western blot of polyubiquitin chains (Ub3, Ub5, Ub8) (lane A), purified ubiquitin (lane B), polyubiquitinated proteins from H9c2 cells treated with 10μM MG-132 for 36 h obtained from affinity purification using TUBEs (lane C), and unbound fraction from H9c2 cells after removal of polyubiquitinated proteins (lane D). B) Upper figure, Western blot of free ubiquitin (lane A) and polyubiquitin chains (lane B) with U5379 antibody diluted at 1:100 and 1:2000. Lower figure, Western blot of free ubiquitin (lane A) and polyubiquitin chains (lane B) with FK1 antibody diluted at 1:1000 in BSA. Even when the blots were imaged for long time periods no additional bands were seen.
    Tandem Ubiquitin Binding Entities Tubes, supplied by LifeSensors, used in various techniques. Bioz Stars score: 85/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tandem ubiquitin binding entities tubes/product/LifeSensors
    Average 85 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    tandem ubiquitin binding entities tubes - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    88
    LifeSensors tandem ubiquitin binding entities
    Nuclear Parkin interacts with ERRα. ( a ) 2-D gel image of proteins pulled down from Parkin −/− vs Parkin TG heart lysates using agarose conjugated TUBES. Circles highlight spots picked for identification. ( b ) Protein spots identified by LC-MS/MS. Text in bold plus asterisk indicate a nuclear protein. ( c ) HeLa cells were transfected with NLS-Parkin and Flag-ERRα as indicated. Western blot analysis of input and anti-flag immunoprecipitates for ERRα and NLS-Parkin. ( d ) Representative Western blot of input and anti-Flag immunoprecipitation using <t>anti-ubiquitin.</t> All immunoprecipitation experiments were repeated at least four different times with similar results. ( e ) Representative Western blot and quantification of protein levels of endogenous ERRα normalized to GAPDH in HeLa cells expressing mCherry, Parkin, or NLS-Parkin (n = 5, *p
    Tandem Ubiquitin Binding Entities, supplied by LifeSensors, used in various techniques. Bioz Stars score: 88/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tandem ubiquitin binding entities/product/LifeSensors
    Average 88 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    tandem ubiquitin binding entities - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    93
    Thermo Fisher centrifuge tubes
    Nuclear Parkin interacts with ERRα. ( a ) 2-D gel image of proteins pulled down from Parkin −/− vs Parkin TG heart lysates using agarose conjugated TUBES. Circles highlight spots picked for identification. ( b ) Protein spots identified by LC-MS/MS. Text in bold plus asterisk indicate a nuclear protein. ( c ) HeLa cells were transfected with NLS-Parkin and Flag-ERRα as indicated. Western blot analysis of input and anti-flag immunoprecipitates for ERRα and NLS-Parkin. ( d ) Representative Western blot of input and anti-Flag immunoprecipitation using <t>anti-ubiquitin.</t> All immunoprecipitation experiments were repeated at least four different times with similar results. ( e ) Representative Western blot and quantification of protein levels of endogenous ERRα normalized to GAPDH in HeLa cells expressing mCherry, Parkin, or NLS-Parkin (n = 5, *p
    Centrifuge Tubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/centrifuge tubes/product/Thermo Fisher
    Average 93 stars, based on 737 article reviews
    Price from $9.99 to $1999.99
    centrifuge tubes - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Validation of anti-ubiquitin antibodies. VU101 in the presence and absence of 0.5% glutaraldehyde pre-treatment, U5379, AP1228a, or P4G7-H11 were used to detect ubiquitin and ubiquitinated proteins. A) Western blot of polyubiquitin chains (Ub3, Ub5, Ub8) (lane A), purified ubiquitin (lane B), polyubiquitinated proteins from H9c2 cells treated with 10μM MG-132 for 36 h obtained from affinity purification using TUBEs (lane C), and unbound fraction from H9c2 cells after removal of polyubiquitinated proteins (lane D). B) Upper figure, Western blot of free ubiquitin (lane A) and polyubiquitin chains (lane B) with U5379 antibody diluted at 1:100 and 1:2000. Lower figure, Western blot of free ubiquitin (lane A) and polyubiquitin chains (lane B) with FK1 antibody diluted at 1:1000 in BSA. Even when the blots were imaged for long time periods no additional bands were seen.

    Journal: PLoS ONE

    Article Title: Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS)

    doi: 10.1371/journal.pone.0135392

    Figure Lengend Snippet: Validation of anti-ubiquitin antibodies. VU101 in the presence and absence of 0.5% glutaraldehyde pre-treatment, U5379, AP1228a, or P4G7-H11 were used to detect ubiquitin and ubiquitinated proteins. A) Western blot of polyubiquitin chains (Ub3, Ub5, Ub8) (lane A), purified ubiquitin (lane B), polyubiquitinated proteins from H9c2 cells treated with 10μM MG-132 for 36 h obtained from affinity purification using TUBEs (lane C), and unbound fraction from H9c2 cells after removal of polyubiquitinated proteins (lane D). B) Upper figure, Western blot of free ubiquitin (lane A) and polyubiquitin chains (lane B) with U5379 antibody diluted at 1:100 and 1:2000. Lower figure, Western blot of free ubiquitin (lane A) and polyubiquitin chains (lane B) with FK1 antibody diluted at 1:1000 in BSA. Even when the blots were imaged for long time periods no additional bands were seen.

    Article Snippet: After centrifugation (14,000xg) for 10 min at 4°C, the supernatant was incubated with Tandem Ubiquitin Binding Entities (TUBEs) bound to agarose (TUBE2-agarose) (UM402, obtained from LifeSensors, PA, USA).

    Techniques: Western Blot, Purification, Affinity Purification

    Comparison of anti-ubiquitin antibodies. Heart and liver lysates (20 μg each) were investigated by Western blotting using five commercially available anti-ubiquitin antibodies (VU101, U5379, AP1228a, P4G7-H11, FK1). Arrow shows location of free unbound ubiquitin. Stain-free staining of total proteins loaded was used as the normalization control. H, heart; L, liver. BSA was used as the blocking reagent for the blot labeled FK1* while non-fat milk was used as the blocking reagent in all the other blots shown. All antibodies were used at a dilution of 1:1000 except for blots labeled U5379* and U5379^ which were used at dilutions of 1:100 and 1:2000 respectively.

    Journal: PLoS ONE

    Article Title: Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS)

    doi: 10.1371/journal.pone.0135392

    Figure Lengend Snippet: Comparison of anti-ubiquitin antibodies. Heart and liver lysates (20 μg each) were investigated by Western blotting using five commercially available anti-ubiquitin antibodies (VU101, U5379, AP1228a, P4G7-H11, FK1). Arrow shows location of free unbound ubiquitin. Stain-free staining of total proteins loaded was used as the normalization control. H, heart; L, liver. BSA was used as the blocking reagent for the blot labeled FK1* while non-fat milk was used as the blocking reagent in all the other blots shown. All antibodies were used at a dilution of 1:1000 except for blots labeled U5379* and U5379^ which were used at dilutions of 1:100 and 1:2000 respectively.

    Article Snippet: After centrifugation (14,000xg) for 10 min at 4°C, the supernatant was incubated with Tandem Ubiquitin Binding Entities (TUBEs) bound to agarose (TUBE2-agarose) (UM402, obtained from LifeSensors, PA, USA).

    Techniques: Western Blot, Staining, Blocking Assay, Labeling

    Nuclear Parkin interacts with ERRα. ( a ) 2-D gel image of proteins pulled down from Parkin −/− vs Parkin TG heart lysates using agarose conjugated TUBES. Circles highlight spots picked for identification. ( b ) Protein spots identified by LC-MS/MS. Text in bold plus asterisk indicate a nuclear protein. ( c ) HeLa cells were transfected with NLS-Parkin and Flag-ERRα as indicated. Western blot analysis of input and anti-flag immunoprecipitates for ERRα and NLS-Parkin. ( d ) Representative Western blot of input and anti-Flag immunoprecipitation using anti-ubiquitin. All immunoprecipitation experiments were repeated at least four different times with similar results. ( e ) Representative Western blot and quantification of protein levels of endogenous ERRα normalized to GAPDH in HeLa cells expressing mCherry, Parkin, or NLS-Parkin (n = 5, *p

    Journal: Scientific Reports

    Article Title: Nuclear Parkin Activates the ERRα Transcriptional Program and Drives Widespread Changes in Gene Expression Following Hypoxia

    doi: 10.1038/s41598-020-65438-7

    Figure Lengend Snippet: Nuclear Parkin interacts with ERRα. ( a ) 2-D gel image of proteins pulled down from Parkin −/− vs Parkin TG heart lysates using agarose conjugated TUBES. Circles highlight spots picked for identification. ( b ) Protein spots identified by LC-MS/MS. Text in bold plus asterisk indicate a nuclear protein. ( c ) HeLa cells were transfected with NLS-Parkin and Flag-ERRα as indicated. Western blot analysis of input and anti-flag immunoprecipitates for ERRα and NLS-Parkin. ( d ) Representative Western blot of input and anti-Flag immunoprecipitation using anti-ubiquitin. All immunoprecipitation experiments were repeated at least four different times with similar results. ( e ) Representative Western blot and quantification of protein levels of endogenous ERRα normalized to GAPDH in HeLa cells expressing mCherry, Parkin, or NLS-Parkin (n = 5, *p

    Article Snippet: Briefly, whole heart lysates were made fresh from Parkin−/− and Parkin-TG mice, pre-cleared with unconjugated agarose beads, and combined with agarose beads bound to tandem ubiquitin binding entities (TUBES, LifeSensors), and pulled down via centrifugation.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Transfection, Western Blot, Immunoprecipitation, Expressing