cenp-b dbd Search Results


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  • 99
    Millipore nde i
    Nde I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11891 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad preparative non denaturing polyacrylamide gel electrophoresis
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    Preparative Non Denaturing Polyacrylamide Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GE Healthcare thrombin protease
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    Thrombin Protease, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1440 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad prep cell apparatus
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    Prep Cell Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen nickel nitrilotriacetic acid ni nta agarose chromatography
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    Nickel Nitrilotriacetic Acid Ni Nta Agarose Chromatography, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher r125a
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    R125a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    GE Healthcare mono s column chromatography
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    Mono S Column Chromatography, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore pet15b vector
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    Pet15b Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 4010 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher human cell line expression
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    Human Cell Line Expression, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Stratagene pcmv tag2a
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    Pcmv Tag2a, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa pegfp c2 mammalian expression vectors
    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by <t>non-denaturing</t> 6% <t>polyacrylamide</t> <t>gel</t> <t>electrophoresis</t> with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.
    Pegfp C2 Mammalian Expression Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by non-denaturing 6% polyacrylamide gel electrophoresis with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.

    Journal: Nucleic Acids Research

    Article Title: Stable complex formation of CENP-B with the CENP-A nucleosome

    doi: 10.1093/nar/gkv405

    Figure Lengend Snippet: CENP-B binds to the CENP-A and H3.1 nucleosomes. ( A ) Schematic representation of CENP-B DBD binding to nucleosomes. ( B ) Electrophoretic mobility shift assay. The H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 1, 3 and 5, respectively) and those complexed with the CENP-B DBD (lanes 2, 4 and 6, respectively) were analyzed by non-denaturing 6% polyacrylamide gel electrophoresis with ethidium bromide staining. ( C ) Protein contents of the H3.1, CENP-A and H3.1 CATD nucleosomes (lanes 2, 4 and 6, respectively) and those complexed with the CENP-B DBD (lanes 3, 5 and 7, respectively), analyzed by SDS-15% polyacrylamide gel electrophoresis with Coomassie Brilliant Blue staining.

    Article Snippet: The nucleosomes complexed with the CENP-B DBD were separated from the free nucleosomes and the CENP-B DBD by preparative non-denaturing polyacrylamide gel electrophoresis (Prep Cell Model 491: Bio-Rad).

    Techniques: Binding Assay, Electrophoretic Mobility Shift Assay, Polyacrylamide Gel Electrophoresis, Staining

    CENP-B binds more stably to the proximal DNA region of the CENP-A nucleosome. ( A ) Schematic representation of the proximal and distal CENP-B box locations, relative to the CENP-A nucleosome (dotted ellipses). The upper and lower panels illustrate the nucleosomes with the 166 base-pair α-satellite DNA (used in Figures 1 and 2 ) and the 166 base-pair α-satellite (-20) DNA, respectively. ( B ) Electrophoretic mobility shift assay. The H3.1 and CENP-A nucleosomes (lanes 1, 3, 5 and 7) and those complexed with the CENP-B DBD (lanes 2, 4, 6 and 8, respectively) were analyzed by non-denaturing 6% polyacrylamide gel electrophoresis with ethidium bromide staining. Lanes 1, 2, 5 and 6 indicate the H3.1 nucleosomes, and lanes 3, 4, 7 and 8 indicate the CENP-A nucleosomes. Lanes 1–4 and lanes 5–8 are experiments with the 166 base-pair α-satellite DNA and the 166 base-pair α-satellite (-20) DNA, respectively. ( C ) The H3.1 (containing 100 ng DNA) complexed with the CENP-B DBD were incubated in the presence of the naked 166 base-pair α-satellite DNA. The amounts of naked 166 base-pair α-satellite DNA are 0 ng (lanes 2 and 8), 50 ng (lanes 3 and 9), 75 ng (lanes 4 and 10), 100 ng (lanes 5 and 11) and 125 ng (lanes 6 and 12). Lanes 1 and 7 indicate control experiments without the CENP-B DBD and the naked 166 base-pair DNA. ( D ) Graphic representation of the experiments shown in panel (C). The amounts (%) of H3.1 nucleosomes complexed with CENP-B DBD were plotted against the amounts of competitor DNA. Averages of three independent experiments are shown with standard deviation values. ( E ) The CENP-A nucleosomes (containing 100 ng DNA) complexed with the CENP-B DBD were incubated in the presence of the naked 166 base-pair α-satellite DNA. The amounts of naked 166 base-pair α-satellite DNA are 0 ng (lanes 2 and 8), 50 ng (lanes 3 and 9), 75 ng (lanes 4 and 10), 100 ng (lanes 5 and 11) and 125 ng (lanes 6 and 12). Lanes 1 and 7 indicate control experiments without the CENP-B DBD and the naked 166 base-pair DNA. ( F ) Graphic representation of the experiments shown in panel (E). The amounts (%) of CENP-A nucleosomes complexed with CENP-B DBD were plotted against the amounts of competitor DNA. Averages of three independent experiments are shown with standard deviation values.

    Journal: Nucleic Acids Research

    Article Title: Stable complex formation of CENP-B with the CENP-A nucleosome

    doi: 10.1093/nar/gkv405

    Figure Lengend Snippet: CENP-B binds more stably to the proximal DNA region of the CENP-A nucleosome. ( A ) Schematic representation of the proximal and distal CENP-B box locations, relative to the CENP-A nucleosome (dotted ellipses). The upper and lower panels illustrate the nucleosomes with the 166 base-pair α-satellite DNA (used in Figures 1 and 2 ) and the 166 base-pair α-satellite (-20) DNA, respectively. ( B ) Electrophoretic mobility shift assay. The H3.1 and CENP-A nucleosomes (lanes 1, 3, 5 and 7) and those complexed with the CENP-B DBD (lanes 2, 4, 6 and 8, respectively) were analyzed by non-denaturing 6% polyacrylamide gel electrophoresis with ethidium bromide staining. Lanes 1, 2, 5 and 6 indicate the H3.1 nucleosomes, and lanes 3, 4, 7 and 8 indicate the CENP-A nucleosomes. Lanes 1–4 and lanes 5–8 are experiments with the 166 base-pair α-satellite DNA and the 166 base-pair α-satellite (-20) DNA, respectively. ( C ) The H3.1 (containing 100 ng DNA) complexed with the CENP-B DBD were incubated in the presence of the naked 166 base-pair α-satellite DNA. The amounts of naked 166 base-pair α-satellite DNA are 0 ng (lanes 2 and 8), 50 ng (lanes 3 and 9), 75 ng (lanes 4 and 10), 100 ng (lanes 5 and 11) and 125 ng (lanes 6 and 12). Lanes 1 and 7 indicate control experiments without the CENP-B DBD and the naked 166 base-pair DNA. ( D ) Graphic representation of the experiments shown in panel (C). The amounts (%) of H3.1 nucleosomes complexed with CENP-B DBD were plotted against the amounts of competitor DNA. Averages of three independent experiments are shown with standard deviation values. ( E ) The CENP-A nucleosomes (containing 100 ng DNA) complexed with the CENP-B DBD were incubated in the presence of the naked 166 base-pair α-satellite DNA. The amounts of naked 166 base-pair α-satellite DNA are 0 ng (lanes 2 and 8), 50 ng (lanes 3 and 9), 75 ng (lanes 4 and 10), 100 ng (lanes 5 and 11) and 125 ng (lanes 6 and 12). Lanes 1 and 7 indicate control experiments without the CENP-B DBD and the naked 166 base-pair DNA. ( F ) Graphic representation of the experiments shown in panel (E). The amounts (%) of CENP-A nucleosomes complexed with CENP-B DBD were plotted against the amounts of competitor DNA. Averages of three independent experiments are shown with standard deviation values.

    Article Snippet: The nucleosomes complexed with the CENP-B DBD were separated from the free nucleosomes and the CENP-B DBD by preparative non-denaturing polyacrylamide gel electrophoresis (Prep Cell Model 491: Bio-Rad).

    Techniques: Stable Transfection, Electrophoretic Mobility Shift Assay, Polyacrylamide Gel Electrophoresis, Staining, Incubation, Standard Deviation