celltox green cytotoxicity Search Results


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  • 99
    Promega celltox green cytotoxicity assay
    Cytotoxic and apoptotic effects of Oncamex. Initial evidence on the mechanism of action of Oncamex was obtained from study of breast cancer cell line models MCF-7 and MDA-MB-231. Microscopic visualisation of MCF-7 cells treated with 30 μ M Oncamex showed a reduction in cell density, changes in cell morphology and the appearance of apoptotic and dead cells after 24 h ( A – C ), which was generalised by 72 h after treatment ( D ). <t>CellTox</t> Green plate assays supported preliminary results by direct measurement of the induction of cytotoxicity in treated MDA-MB-231 cells, peaking 8 h after treatment ( E ). Western blotting was used to measure PARP cleavage in treated MCF-7 cells ( F ): Oncamex seemed to induce apoptosis faster, with PARP cleavage occurring after 8 h treatment with the highest concentrations, whereas 24-h incubation was required with AO-1530. The 24-h incubation with the highest concentrations seemed to induce further cell degradation, preventing protein detection. Apoptosis was confirmed by an additional method using ApoTox plate assays, which reported an dose-dependent increase in cytotoxicity and apoptosis, inversely proportional to cell viability ( G ). These antitumour effects were partially blocked by 30 pre-treatment with 10 m M of the antioxidant NAC ( H ).
    Celltox Green Cytotoxicity Assay, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltox green cytotoxicity assay/product/Promega
    Average 99 stars, based on 633 article reviews
    Price from $9.99 to $1999.99
    celltox green cytotoxicity assay - by Bioz Stars, 2020-08
    99/100 stars
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    91
    Promega celltox green cytotoxicity assay kit
    Functional consequences of E152K STIM1 in rat pancreatic acinar cells. (A) Average values of the speed (left hand panel) and amplitude (right hand panel) of ER Ca 2+ release induced by the addition of 1 nM CCK in AR42J cells transfected with E152K STIM1, WT STIM1 or an empty vector. Values were normalized to WT STIM1. n = 449 (empty vector), 705 (WT) or 512 (E152K). ( B ) Left hand panel, quantification of BZiPAR fluorescence at different time points in cells expressing WT STIM1, E152K STIM1 or empty vector after CCK treatment (1 nM). Right hand panel, representative images of BZiPAR fluorescence in E152K STIM1-expressing cells, before and after 4h of CCK treatment. Cells were excited at 494 nm for 150 ms, and fluorescence emission was collected at 535 nm. Scale bar, 50 µM. ( C ) Analysis by Western blot of PRSS1 intracellular expression and its presence in the conditioned medium before and after stimulation with CCK (1 nM) of cells expressing E152K or WT STIM1. ( D ) Quantification over time of fluorescence signal evaluating cytotoxicity <t>(celltox)</t> in cells transfected with E152K STIM1, T153I STIM1, WT STIM1 or an empty vector after CCK addition (1 nM). Cell toxicity was measured using the CellTox™ Green Cytotoxicity Assay kit (Promega), and results were normalized to living cell numbers as evaluated with the CellTiter 96 ® AQueous One Solution Cell Proliferation Assay Kit (Promega) following the manufacturer’s instructions.
    Celltox Green Cytotoxicity Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltox green cytotoxicity assay kit/product/Promega
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    celltox green cytotoxicity assay kit - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    Promega celltox green ldh cytotoxicity detection kit
    Functional consequences of E152K STIM1 in rat pancreatic acinar cells. (A) Average values of the speed (left hand panel) and amplitude (right hand panel) of ER Ca 2+ release induced by the addition of 1 nM CCK in AR42J cells transfected with E152K STIM1, WT STIM1 or an empty vector. Values were normalized to WT STIM1. n = 449 (empty vector), 705 (WT) or 512 (E152K). ( B ) Left hand panel, quantification of BZiPAR fluorescence at different time points in cells expressing WT STIM1, E152K STIM1 or empty vector after CCK treatment (1 nM). Right hand panel, representative images of BZiPAR fluorescence in E152K STIM1-expressing cells, before and after 4h of CCK treatment. Cells were excited at 494 nm for 150 ms, and fluorescence emission was collected at 535 nm. Scale bar, 50 µM. ( C ) Analysis by Western blot of PRSS1 intracellular expression and its presence in the conditioned medium before and after stimulation with CCK (1 nM) of cells expressing E152K or WT STIM1. ( D ) Quantification over time of fluorescence signal evaluating cytotoxicity <t>(celltox)</t> in cells transfected with E152K STIM1, T153I STIM1, WT STIM1 or an empty vector after CCK addition (1 nM). Cell toxicity was measured using the CellTox™ Green Cytotoxicity Assay kit (Promega), and results were normalized to living cell numbers as evaluated with the CellTiter 96 ® AQueous One Solution Cell Proliferation Assay Kit (Promega) following the manufacturer’s instructions.
    Celltox Green Ldh Cytotoxicity Detection Kit, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/celltox green ldh cytotoxicity detection kit/product/Promega
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    celltox green ldh cytotoxicity detection kit - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Cytotoxic and apoptotic effects of Oncamex. Initial evidence on the mechanism of action of Oncamex was obtained from study of breast cancer cell line models MCF-7 and MDA-MB-231. Microscopic visualisation of MCF-7 cells treated with 30 μ M Oncamex showed a reduction in cell density, changes in cell morphology and the appearance of apoptotic and dead cells after 24 h ( A – C ), which was generalised by 72 h after treatment ( D ). CellTox Green plate assays supported preliminary results by direct measurement of the induction of cytotoxicity in treated MDA-MB-231 cells, peaking 8 h after treatment ( E ). Western blotting was used to measure PARP cleavage in treated MCF-7 cells ( F ): Oncamex seemed to induce apoptosis faster, with PARP cleavage occurring after 8 h treatment with the highest concentrations, whereas 24-h incubation was required with AO-1530. The 24-h incubation with the highest concentrations seemed to induce further cell degradation, preventing protein detection. Apoptosis was confirmed by an additional method using ApoTox plate assays, which reported an dose-dependent increase in cytotoxicity and apoptosis, inversely proportional to cell viability ( G ). These antitumour effects were partially blocked by 30 pre-treatment with 10 m M of the antioxidant NAC ( H ).

    Journal: British Journal of Cancer

    Article Title: Antitumour activity of the novel flavonoid Oncamex in preclinical breast cancer models

    doi: 10.1038/bjc.2016.6

    Figure Lengend Snippet: Cytotoxic and apoptotic effects of Oncamex. Initial evidence on the mechanism of action of Oncamex was obtained from study of breast cancer cell line models MCF-7 and MDA-MB-231. Microscopic visualisation of MCF-7 cells treated with 30 μ M Oncamex showed a reduction in cell density, changes in cell morphology and the appearance of apoptotic and dead cells after 24 h ( A – C ), which was generalised by 72 h after treatment ( D ). CellTox Green plate assays supported preliminary results by direct measurement of the induction of cytotoxicity in treated MDA-MB-231 cells, peaking 8 h after treatment ( E ). Western blotting was used to measure PARP cleavage in treated MCF-7 cells ( F ): Oncamex seemed to induce apoptosis faster, with PARP cleavage occurring after 8 h treatment with the highest concentrations, whereas 24-h incubation was required with AO-1530. The 24-h incubation with the highest concentrations seemed to induce further cell degradation, preventing protein detection. Apoptosis was confirmed by an additional method using ApoTox plate assays, which reported an dose-dependent increase in cytotoxicity and apoptosis, inversely proportional to cell viability ( G ). These antitumour effects were partially blocked by 30 pre-treatment with 10 m M of the antioxidant NAC ( H ).

    Article Snippet: Plate-based assays The CellTox Green Cytotoxicity and ApoTox-Glo Triplex assay kits (Promega, Madison, WI, USA) were used to measure cytotoxicity at different timepoints and to assess the mechanism of action of the drugs studied, respectively.

    Techniques: Multiple Displacement Amplification, Western Blot, Incubation

    In vitro invasion and proliferation of gapA, gapA -complemented, and wt FSC200 in bone marrow macrophages (BMMs) (A) and A549 cells (B) . The cells were infected at MOI of 50:1 (BMM) or 200:1 (A549) with the indicated strains. BMMs were harvested at 1, 6, 12, 24, and 48 h and A549 at 4, 24, and 48 h post-infection. The numbers of bacteria recovered from the cells were counted as cfu. The data represent means ± SD of three independent experiments performed in triplicate. (C) Viability of cells and induction of cytotoxicity determined in BMMs infected with gapA mutant or wild-type FSC 200 strains. At 2, 24, and 48 h the cells were assayed using RealTime-Glo™ MT Cell Viability Assay kit and CellTox™ Green Cytotoxicity Assay kit (Promega). Data are means ± SD of triplicate samples and the results shown are representatives of three independent experiments. Asterisks indicate statistically significant differences; ** P

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The Multiple Localized Glyceraldehyde-3-Phosphate Dehydrogenase Contributes to the Attenuation of the Francisella tularensis dsbA Deletion Mutant

    doi: 10.3389/fcimb.2017.00503

    Figure Lengend Snippet: In vitro invasion and proliferation of gapA, gapA -complemented, and wt FSC200 in bone marrow macrophages (BMMs) (A) and A549 cells (B) . The cells were infected at MOI of 50:1 (BMM) or 200:1 (A549) with the indicated strains. BMMs were harvested at 1, 6, 12, 24, and 48 h and A549 at 4, 24, and 48 h post-infection. The numbers of bacteria recovered from the cells were counted as cfu. The data represent means ± SD of three independent experiments performed in triplicate. (C) Viability of cells and induction of cytotoxicity determined in BMMs infected with gapA mutant or wild-type FSC 200 strains. At 2, 24, and 48 h the cells were assayed using RealTime-Glo™ MT Cell Viability Assay kit and CellTox™ Green Cytotoxicity Assay kit (Promega). Data are means ± SD of triplicate samples and the results shown are representatives of three independent experiments. Asterisks indicate statistically significant differences; ** P

    Article Snippet: The cytotoxicity was assayed in parallel using fluorescence CellTox™ Green Cytotoxicity Assay (Promega).

    Techniques: In Vitro, Infection, Mutagenesis, Viability Assay, Cytotoxicity Assay

    The effect of fragmented fibril on cell viability by cell membrane integrity assay. SH-SY5Y cells (2x10 4 /well) were plated with the addition of the CellTox dye and allowed to adhere. The unfragmented and fragmented fibril samples were then added (A) α-syn 7 μM and (B) Aβ 40 10 μM. Fluorescence was recorded over a period of 72 hours (520 Em / 485 Ex ). Light grey bars denote unfragmented fibril samples and dark grey bars denote fragmented fibril samples (n = 3, error bars show SE). Unfragmented (light grey) and fragmented fibril samples (dark grey) (n = 6, SE). Statistical significance between toxicity of unfragmented and fragmented fibrils at each time point was determined by a Mann Whitney U test P ≤ 0.05 (*) or P ≤ 0.005 (**).

    Journal: PLoS ONE

    Article Title: Analysis of Toxic Amyloid Fibril Interactions at Natively Derived Membranes by Ellipsometry

    doi: 10.1371/journal.pone.0132309

    Figure Lengend Snippet: The effect of fragmented fibril on cell viability by cell membrane integrity assay. SH-SY5Y cells (2x10 4 /well) were plated with the addition of the CellTox dye and allowed to adhere. The unfragmented and fragmented fibril samples were then added (A) α-syn 7 μM and (B) Aβ 40 10 μM. Fluorescence was recorded over a period of 72 hours (520 Em / 485 Ex ). Light grey bars denote unfragmented fibril samples and dark grey bars denote fragmented fibril samples (n = 3, error bars show SE). Unfragmented (light grey) and fragmented fibril samples (dark grey) (n = 6, SE). Statistical significance between toxicity of unfragmented and fragmented fibrils at each time point was determined by a Mann Whitney U test P ≤ 0.05 (*) or P ≤ 0.005 (**).

    Article Snippet: A direct result of membrane disruption in vivo would lead to a loss in cell viability, which was confirmed by the CellTox Green assay (Promega) ( ).

    Techniques: Integrity Assay, Fluorescence, MANN-WHITNEY

    Functional consequences of E152K STIM1 in rat pancreatic acinar cells. (A) Average values of the speed (left hand panel) and amplitude (right hand panel) of ER Ca 2+ release induced by the addition of 1 nM CCK in AR42J cells transfected with E152K STIM1, WT STIM1 or an empty vector. Values were normalized to WT STIM1. n = 449 (empty vector), 705 (WT) or 512 (E152K). ( B ) Left hand panel, quantification of BZiPAR fluorescence at different time points in cells expressing WT STIM1, E152K STIM1 or empty vector after CCK treatment (1 nM). Right hand panel, representative images of BZiPAR fluorescence in E152K STIM1-expressing cells, before and after 4h of CCK treatment. Cells were excited at 494 nm for 150 ms, and fluorescence emission was collected at 535 nm. Scale bar, 50 µM. ( C ) Analysis by Western blot of PRSS1 intracellular expression and its presence in the conditioned medium before and after stimulation with CCK (1 nM) of cells expressing E152K or WT STIM1. ( D ) Quantification over time of fluorescence signal evaluating cytotoxicity (celltox) in cells transfected with E152K STIM1, T153I STIM1, WT STIM1 or an empty vector after CCK addition (1 nM). Cell toxicity was measured using the CellTox™ Green Cytotoxicity Assay kit (Promega), and results were normalized to living cell numbers as evaluated with the CellTiter 96 ® AQueous One Solution Cell Proliferation Assay Kit (Promega) following the manufacturer’s instructions.

    Journal: bioRxiv

    Article Title: p.E152K-STIM1 mutation deregulates Ca2+ signaling contributing to chronic pancreatitis

    doi: 10.1101/2020.01.22.916254

    Figure Lengend Snippet: Functional consequences of E152K STIM1 in rat pancreatic acinar cells. (A) Average values of the speed (left hand panel) and amplitude (right hand panel) of ER Ca 2+ release induced by the addition of 1 nM CCK in AR42J cells transfected with E152K STIM1, WT STIM1 or an empty vector. Values were normalized to WT STIM1. n = 449 (empty vector), 705 (WT) or 512 (E152K). ( B ) Left hand panel, quantification of BZiPAR fluorescence at different time points in cells expressing WT STIM1, E152K STIM1 or empty vector after CCK treatment (1 nM). Right hand panel, representative images of BZiPAR fluorescence in E152K STIM1-expressing cells, before and after 4h of CCK treatment. Cells were excited at 494 nm for 150 ms, and fluorescence emission was collected at 535 nm. Scale bar, 50 µM. ( C ) Analysis by Western blot of PRSS1 intracellular expression and its presence in the conditioned medium before and after stimulation with CCK (1 nM) of cells expressing E152K or WT STIM1. ( D ) Quantification over time of fluorescence signal evaluating cytotoxicity (celltox) in cells transfected with E152K STIM1, T153I STIM1, WT STIM1 or an empty vector after CCK addition (1 nM). Cell toxicity was measured using the CellTox™ Green Cytotoxicity Assay kit (Promega), and results were normalized to living cell numbers as evaluated with the CellTiter 96 ® AQueous One Solution Cell Proliferation Assay Kit (Promega) following the manufacturer’s instructions.

    Article Snippet: Cytotoxicity assay Cell toxicity was measured using the CellTox™ Green Cytotoxicity Assay kit (Promega), and results were normalized to living cell numbers as evaluated with the CellTiter 96® AQueous One Solution Cell Proliferation Assay Kit (Promega) following the manufacturer’s instructions.

    Techniques: Functional Assay, Transfection, Plasmid Preparation, Fluorescence, Expressing, Western Blot, Cytotoxicity Assay, Proliferation Assay