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  • 90
    Thermo Fisher mmp 9
    Impact of azithromycin (AZM) upon <t>MMP-9.</t> (a) Macrophages were co-cultured with fibroblasts and treated with IFNγ, IL-4/IL-13 or medium alone overnight. At time 0, AZM and/or P. aeruginosa (PA) were added and the cells were incubated for 4 h. Supernatants
    Mmp 9, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1977 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorescence
    Impact of azithromycin (AZM) upon <t>MMP-9.</t> (a) Macrophages were co-cultured with fibroblasts and treated with IFNγ, IL-4/IL-13 or medium alone overnight. At time 0, AZM and/or P. aeruginosa (PA) were added and the cells were incubated for 4 h. Supernatants
    Fluorescence, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cells kit
    Impact of azithromycin (AZM) upon <t>MMP-9.</t> (a) Macrophages were co-cultured with fibroblasts and treated with IFNγ, IL-4/IL-13 or medium alone overnight. At time 0, AZM and/or P. aeruginosa (PA) were added and the cells were incubated for 4 h. Supernatants
    Cells Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Applications Inc hbec cells
    Impact of azithromycin (AZM) upon <t>MMP-9.</t> (a) Macrophages were co-cultured with fibroblasts and treated with IFNγ, IL-4/IL-13 or medium alone overnight. At time 0, AZM and/or P. aeruginosa (PA) were added and the cells were incubated for 4 h. Supernatants
    Hbec Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mammalian cells
    Impact of azithromycin (AZM) upon <t>MMP-9.</t> (a) Macrophages were co-cultured with fibroblasts and treated with IFNγ, IL-4/IL-13 or medium alone overnight. At time 0, AZM and/or P. aeruginosa (PA) were added and the cells were incubated for 4 h. Supernatants
    Mammalian Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher raji cells
    Impact of azithromycin (AZM) upon <t>MMP-9.</t> (a) Macrophages were co-cultured with fibroblasts and treated with IFNγ, IL-4/IL-13 or medium alone overnight. At time 0, AZM and/or P. aeruginosa (PA) were added and the cells were incubated for 4 h. Supernatants
    Raji Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Applications Inc bce cells
    Impact of azithromycin (AZM) upon <t>MMP-9.</t> (a) Macrophages were co-cultured with fibroblasts and treated with IFNγ, IL-4/IL-13 or medium alone overnight. At time 0, AZM and/or P. aeruginosa (PA) were added and the cells were incubated for 4 h. Supernatants
    Bce Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 80/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Applications Inc hdlmvec cells
    AR of ccRCC cells influences angiogenesis and lymphoangiogenesis. a Outline of co-culture system. Endothelial cells (HUVEC and <t>HDLMVEC)</t> were co-cultured with ccRCC cells with indicated treatments (with/without overexpression/knockdown of AR) for 72 h, and then the endothelial cells were harvested. After harvest, the endothelial cells were maintained in mixed conditioned medium (CM from co-culture system) with fresh media at the ratio of 1:1. b , d Wound-healing assays of HUVEC and HDLMVEC cells co-cultured with A498 b and SW839 d with altered AR expression at indicated time points. Scale bar in b , d 10 μm. c , e Quantitation of endothelial cells migration in wound-healing assay after co-culture with indicated ccRCC cells as described in b , d . f , h Transwell invasion assay of HUVEC ( upper ) and HDLMVEC ( lower ) after co-culture with A498 cells overexpressing AR ( f ) and SW839 cells with AR knocked down ( h ). After 24 h, the invaded cells were stained and five random fields per well were analyzed. Scale bar in f , h , 5 μm. g , i Quantitation of the transwell invasion described in f , h . j A representative image of tube formation assays of HUVEC and HDLMVEC after co-culture with A498 overexpressing AR. The HUVEC cells during coculture with A498 cells were also blocked with VEGF-A neutralizing antibody (R D Systems) at the concentration of 0.08 µg/ml. The endothelial cells were pre-stained with Calcein AM at the concentration of 2 µM and monitored with fluorescence microscope. l Tube formation assay of HUVEC and HDLMVEC after co-culture with SW839 cells with AR knockdown. The SW839 cells were infected with lentivirus for AR and sh-VEGF-C were used for co-culture with HDLMVEC cells for the rescue assay. Scale bar in j , l , 10 μm. k , m Quantitation of the tube number described in j , l . The functional assays were all replicated four times. * p
    Hdlmvec Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher assay buffer
    AR of ccRCC cells influences angiogenesis and lymphoangiogenesis. a Outline of co-culture system. Endothelial cells (HUVEC and <t>HDLMVEC)</t> were co-cultured with ccRCC cells with indicated treatments (with/without overexpression/knockdown of AR) for 72 h, and then the endothelial cells were harvested. After harvest, the endothelial cells were maintained in mixed conditioned medium (CM from co-culture system) with fresh media at the ratio of 1:1. b , d Wound-healing assays of HUVEC and HDLMVEC cells co-cultured with A498 b and SW839 d with altered AR expression at indicated time points. Scale bar in b , d 10 μm. c , e Quantitation of endothelial cells migration in wound-healing assay after co-culture with indicated ccRCC cells as described in b , d . f , h Transwell invasion assay of HUVEC ( upper ) and HDLMVEC ( lower ) after co-culture with A498 cells overexpressing AR ( f ) and SW839 cells with AR knocked down ( h ). After 24 h, the invaded cells were stained and five random fields per well were analyzed. Scale bar in f , h , 5 μm. g , i Quantitation of the transwell invasion described in f , h . j A representative image of tube formation assays of HUVEC and HDLMVEC after co-culture with A498 overexpressing AR. The HUVEC cells during coculture with A498 cells were also blocked with VEGF-A neutralizing antibody (R D Systems) at the concentration of 0.08 µg/ml. The endothelial cells were pre-stained with Calcein AM at the concentration of 2 µM and monitored with fluorescence microscope. l Tube formation assay of HUVEC and HDLMVEC after co-culture with SW839 cells with AR knockdown. The SW839 cells were infected with lentivirus for AR and sh-VEGF-C were used for co-culture with HDLMVEC cells for the rescue assay. Scale bar in j , l , 10 μm. k , m Quantitation of the tube number described in j , l . The functional assays were all replicated four times. * p
    Assay Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beckman Coulter moflo cell sorter
    AR of ccRCC cells influences angiogenesis and lymphoangiogenesis. a Outline of co-culture system. Endothelial cells (HUVEC and <t>HDLMVEC)</t> were co-cultured with ccRCC cells with indicated treatments (with/without overexpression/knockdown of AR) for 72 h, and then the endothelial cells were harvested. After harvest, the endothelial cells were maintained in mixed conditioned medium (CM from co-culture system) with fresh media at the ratio of 1:1. b , d Wound-healing assays of HUVEC and HDLMVEC cells co-cultured with A498 b and SW839 d with altered AR expression at indicated time points. Scale bar in b , d 10 μm. c , e Quantitation of endothelial cells migration in wound-healing assay after co-culture with indicated ccRCC cells as described in b , d . f , h Transwell invasion assay of HUVEC ( upper ) and HDLMVEC ( lower ) after co-culture with A498 cells overexpressing AR ( f ) and SW839 cells with AR knocked down ( h ). After 24 h, the invaded cells were stained and five random fields per well were analyzed. Scale bar in f , h , 5 μm. g , i Quantitation of the transwell invasion described in f , h . j A representative image of tube formation assays of HUVEC and HDLMVEC after co-culture with A498 overexpressing AR. The HUVEC cells during coculture with A498 cells were also blocked with VEGF-A neutralizing antibody (R D Systems) at the concentration of 0.08 µg/ml. The endothelial cells were pre-stained with Calcein AM at the concentration of 2 µM and monitored with fluorescence microscope. l Tube formation assay of HUVEC and HDLMVEC after co-culture with SW839 cells with AR knockdown. The SW839 cells were infected with lentivirus for AR and sh-VEGF-C were used for co-culture with HDLMVEC cells for the rescue assay. Scale bar in j , l , 10 μm. k , m Quantitation of the tube number described in j , l . The functional assays were all replicated four times. * p
    Moflo Cell Sorter, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 99/100, based on 1967 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sonics & Materials vibra cell vcx130
    AR of ccRCC cells influences angiogenesis and lymphoangiogenesis. a Outline of co-culture system. Endothelial cells (HUVEC and <t>HDLMVEC)</t> were co-cultured with ccRCC cells with indicated treatments (with/without overexpression/knockdown of AR) for 72 h, and then the endothelial cells were harvested. After harvest, the endothelial cells were maintained in mixed conditioned medium (CM from co-culture system) with fresh media at the ratio of 1:1. b , d Wound-healing assays of HUVEC and HDLMVEC cells co-cultured with A498 b and SW839 d with altered AR expression at indicated time points. Scale bar in b , d 10 μm. c , e Quantitation of endothelial cells migration in wound-healing assay after co-culture with indicated ccRCC cells as described in b , d . f , h Transwell invasion assay of HUVEC ( upper ) and HDLMVEC ( lower ) after co-culture with A498 cells overexpressing AR ( f ) and SW839 cells with AR knocked down ( h ). After 24 h, the invaded cells were stained and five random fields per well were analyzed. Scale bar in f , h , 5 μm. g , i Quantitation of the transwell invasion described in f , h . j A representative image of tube formation assays of HUVEC and HDLMVEC after co-culture with A498 overexpressing AR. The HUVEC cells during coculture with A498 cells were also blocked with VEGF-A neutralizing antibody (R D Systems) at the concentration of 0.08 µg/ml. The endothelial cells were pre-stained with Calcein AM at the concentration of 2 µM and monitored with fluorescence microscope. l Tube formation assay of HUVEC and HDLMVEC after co-culture with SW839 cells with AR knockdown. The SW839 cells were infected with lentivirus for AR and sh-VEGF-C were used for co-culture with HDLMVEC cells for the rescue assay. Scale bar in j , l , 10 μm. k , m Quantitation of the tube number described in j , l . The functional assays were all replicated four times. * p
    Vibra Cell Vcx130, supplied by Sonics & Materials, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Applications Inc hek cells
    AR of ccRCC cells influences angiogenesis and lymphoangiogenesis. a Outline of co-culture system. Endothelial cells (HUVEC and <t>HDLMVEC)</t> were co-cultured with ccRCC cells with indicated treatments (with/without overexpression/knockdown of AR) for 72 h, and then the endothelial cells were harvested. After harvest, the endothelial cells were maintained in mixed conditioned medium (CM from co-culture system) with fresh media at the ratio of 1:1. b , d Wound-healing assays of HUVEC and HDLMVEC cells co-cultured with A498 b and SW839 d with altered AR expression at indicated time points. Scale bar in b , d 10 μm. c , e Quantitation of endothelial cells migration in wound-healing assay after co-culture with indicated ccRCC cells as described in b , d . f , h Transwell invasion assay of HUVEC ( upper ) and HDLMVEC ( lower ) after co-culture with A498 cells overexpressing AR ( f ) and SW839 cells with AR knocked down ( h ). After 24 h, the invaded cells were stained and five random fields per well were analyzed. Scale bar in f , h , 5 μm. g , i Quantitation of the transwell invasion described in f , h . j A representative image of tube formation assays of HUVEC and HDLMVEC after co-culture with A498 overexpressing AR. The HUVEC cells during coculture with A498 cells were also blocked with VEGF-A neutralizing antibody (R D Systems) at the concentration of 0.08 µg/ml. The endothelial cells were pre-stained with Calcein AM at the concentration of 2 µM and monitored with fluorescence microscope. l Tube formation assay of HUVEC and HDLMVEC after co-culture with SW839 cells with AR knockdown. The SW839 cells were infected with lentivirus for AR and sh-VEGF-C were used for co-culture with HDLMVEC cells for the rescue assay. Scale bar in j , l , 10 μm. k , m Quantitation of the tube number described in j , l . The functional assays were all replicated four times. * p
    Hek Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqmanfast cells to ct kit
    AR of ccRCC cells influences angiogenesis and lymphoangiogenesis. a Outline of co-culture system. Endothelial cells (HUVEC and <t>HDLMVEC)</t> were co-cultured with ccRCC cells with indicated treatments (with/without overexpression/knockdown of AR) for 72 h, and then the endothelial cells were harvested. After harvest, the endothelial cells were maintained in mixed conditioned medium (CM from co-culture system) with fresh media at the ratio of 1:1. b , d Wound-healing assays of HUVEC and HDLMVEC cells co-cultured with A498 b and SW839 d with altered AR expression at indicated time points. Scale bar in b , d 10 μm. c , e Quantitation of endothelial cells migration in wound-healing assay after co-culture with indicated ccRCC cells as described in b , d . f , h Transwell invasion assay of HUVEC ( upper ) and HDLMVEC ( lower ) after co-culture with A498 cells overexpressing AR ( f ) and SW839 cells with AR knocked down ( h ). After 24 h, the invaded cells were stained and five random fields per well were analyzed. Scale bar in f , h , 5 μm. g , i Quantitation of the transwell invasion described in f , h . j A representative image of tube formation assays of HUVEC and HDLMVEC after co-culture with A498 overexpressing AR. The HUVEC cells during coculture with A498 cells were also blocked with VEGF-A neutralizing antibody (R D Systems) at the concentration of 0.08 µg/ml. The endothelial cells were pre-stained with Calcein AM at the concentration of 2 µM and monitored with fluorescence microscope. l Tube formation assay of HUVEC and HDLMVEC after co-culture with SW839 cells with AR knockdown. The SW839 cells were infected with lentivirus for AR and sh-VEGF-C were used for co-culture with HDLMVEC cells for the rescue assay. Scale bar in j , l , 10 μm. k , m Quantitation of the tube number described in j , l . The functional assays were all replicated four times. * p
    Taqmanfast Cells To Ct Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ambion cells to ct kit
    AR of ccRCC cells influences angiogenesis and lymphoangiogenesis. a Outline of co-culture system. Endothelial cells (HUVEC and <t>HDLMVEC)</t> were co-cultured with ccRCC cells with indicated treatments (with/without overexpression/knockdown of AR) for 72 h, and then the endothelial cells were harvested. After harvest, the endothelial cells were maintained in mixed conditioned medium (CM from co-culture system) with fresh media at the ratio of 1:1. b , d Wound-healing assays of HUVEC and HDLMVEC cells co-cultured with A498 b and SW839 d with altered AR expression at indicated time points. Scale bar in b , d 10 μm. c , e Quantitation of endothelial cells migration in wound-healing assay after co-culture with indicated ccRCC cells as described in b , d . f , h Transwell invasion assay of HUVEC ( upper ) and HDLMVEC ( lower ) after co-culture with A498 cells overexpressing AR ( f ) and SW839 cells with AR knocked down ( h ). After 24 h, the invaded cells were stained and five random fields per well were analyzed. Scale bar in f , h , 5 μm. g , i Quantitation of the transwell invasion described in f , h . j A representative image of tube formation assays of HUVEC and HDLMVEC after co-culture with A498 overexpressing AR. The HUVEC cells during coculture with A498 cells were also blocked with VEGF-A neutralizing antibody (R D Systems) at the concentration of 0.08 µg/ml. The endothelial cells were pre-stained with Calcein AM at the concentration of 2 µM and monitored with fluorescence microscope. l Tube formation assay of HUVEC and HDLMVEC after co-culture with SW839 cells with AR knockdown. The SW839 cells were infected with lentivirus for AR and sh-VEGF-C were used for co-culture with HDLMVEC cells for the rescue assay. Scale bar in j , l , 10 μm. k , m Quantitation of the tube number described in j , l . The functional assays were all replicated four times. * p
    Ambion Cells To Ct Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher silencer select gapdh positive control sirna
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    Silencer Select Gapdh Positive Control Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cell suspension buffer
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    Cell Suspension Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Antibodies Inc hep2 cells
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    Hep2 Cells, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Applications Inc canine osteoblast cells
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    Canine Osteoblast Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 8w10e ecis cell plates
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    8w10e Ecis Cell Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cell lysate
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    Cell Lysate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applied Precision Inc deltavision live cell microscope
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    Deltavision Live Cell Microscope, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 84/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cll cells
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    Cll Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 714 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Verity Software House cell modifit software
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    Cell Modifit Software, supplied by Verity Software House, used in various techniques. Bioz Stars score: 84/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Applications Inc hmec cells
    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of <t>GAPDH-</t> and SR-BI-mRNA bands in negative control <t>siRNA</t> (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications
    Hmec Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Impact of azithromycin (AZM) upon MMP-9. (a) Macrophages were co-cultured with fibroblasts and treated with IFNγ, IL-4/IL-13 or medium alone overnight. At time 0, AZM and/or P. aeruginosa (PA) were added and the cells were incubated for 4 h. Supernatants

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Azithromycin increases in vitro fibronectin production through interactions between macrophages and fibroblasts stimulated with Pseudomonas aeruginosa

    doi: 10.1093/jac/dks476

    Figure Lengend Snippet: Impact of azithromycin (AZM) upon MMP-9. (a) Macrophages were co-cultured with fibroblasts and treated with IFNγ, IL-4/IL-13 or medium alone overnight. At time 0, AZM and/or P. aeruginosa (PA) were added and the cells were incubated for 4 h. Supernatants

    Article Snippet: Reverse transcription was performed on equal amounts of RNA utilizing Taqman Reverse Transcriptase Reagents (Applied Biosystems) according to the manufacturer's protocols. qRT–PCR was initiated utilizing Taqman gene expression arrays for murine TGFβ1, MMP-9 and GAPDH using an ABI Prism 7000 (Applied Biosystems).

    Techniques: Cell Culture, Incubation

    Effect of MMP-9 inhibition upon the activation of TGFβ. Macrophages and fibroblasts were co-cultured, treated with IFNγ and stimulated with LPS in the presence or absence of 30 μM azithromycin (AZM). Cells and supernatants were

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Azithromycin increases in vitro fibronectin production through interactions between macrophages and fibroblasts stimulated with Pseudomonas aeruginosa

    doi: 10.1093/jac/dks476

    Figure Lengend Snippet: Effect of MMP-9 inhibition upon the activation of TGFβ. Macrophages and fibroblasts were co-cultured, treated with IFNγ and stimulated with LPS in the presence or absence of 30 μM azithromycin (AZM). Cells and supernatants were

    Article Snippet: Reverse transcription was performed on equal amounts of RNA utilizing Taqman Reverse Transcriptase Reagents (Applied Biosystems) according to the manufacturer's protocols. qRT–PCR was initiated utilizing Taqman gene expression arrays for murine TGFβ1, MMP-9 and GAPDH using an ABI Prism 7000 (Applied Biosystems).

    Techniques: Inhibition, Activation Assay, Cell Culture

    Expression of MMP-9 and fibronectin in a mouse model of P. aeruginosa pneumonia. C57Bl/6 mice were infected intratracheally with agarose beads containing 1.5 × 10 5 cfu of bacteria. Mice were dosed daily via oral gavage with 0.16

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Azithromycin increases in vitro fibronectin production through interactions between macrophages and fibroblasts stimulated with Pseudomonas aeruginosa

    doi: 10.1093/jac/dks476

    Figure Lengend Snippet: Expression of MMP-9 and fibronectin in a mouse model of P. aeruginosa pneumonia. C57Bl/6 mice were infected intratracheally with agarose beads containing 1.5 × 10 5 cfu of bacteria. Mice were dosed daily via oral gavage with 0.16

    Article Snippet: Reverse transcription was performed on equal amounts of RNA utilizing Taqman Reverse Transcriptase Reagents (Applied Biosystems) according to the manufacturer's protocols. qRT–PCR was initiated utilizing Taqman gene expression arrays for murine TGFβ1, MMP-9 and GAPDH using an ABI Prism 7000 (Applied Biosystems).

    Techniques: Expressing, Mouse Assay, Infection

    Result of TGFβ neutralization on ability of azithromycin (AZM) to affect fibronectin and MMP-9. Macrophages and fibroblasts were co-cultured, treated with IFNγ and stimulated with LPS in the presence or absence of 30 μM AZM. Cells

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Azithromycin increases in vitro fibronectin production through interactions between macrophages and fibroblasts stimulated with Pseudomonas aeruginosa

    doi: 10.1093/jac/dks476

    Figure Lengend Snippet: Result of TGFβ neutralization on ability of azithromycin (AZM) to affect fibronectin and MMP-9. Macrophages and fibroblasts were co-cultured, treated with IFNγ and stimulated with LPS in the presence or absence of 30 μM AZM. Cells

    Article Snippet: Reverse transcription was performed on equal amounts of RNA utilizing Taqman Reverse Transcriptase Reagents (Applied Biosystems) according to the manufacturer's protocols. qRT–PCR was initiated utilizing Taqman gene expression arrays for murine TGFβ1, MMP-9 and GAPDH using an ABI Prism 7000 (Applied Biosystems).

    Techniques: Neutralization, Cell Culture

    AR of ccRCC cells influences angiogenesis and lymphoangiogenesis. a Outline of co-culture system. Endothelial cells (HUVEC and HDLMVEC) were co-cultured with ccRCC cells with indicated treatments (with/without overexpression/knockdown of AR) for 72 h, and then the endothelial cells were harvested. After harvest, the endothelial cells were maintained in mixed conditioned medium (CM from co-culture system) with fresh media at the ratio of 1:1. b , d Wound-healing assays of HUVEC and HDLMVEC cells co-cultured with A498 b and SW839 d with altered AR expression at indicated time points. Scale bar in b , d 10 μm. c , e Quantitation of endothelial cells migration in wound-healing assay after co-culture with indicated ccRCC cells as described in b , d . f , h Transwell invasion assay of HUVEC ( upper ) and HDLMVEC ( lower ) after co-culture with A498 cells overexpressing AR ( f ) and SW839 cells with AR knocked down ( h ). After 24 h, the invaded cells were stained and five random fields per well were analyzed. Scale bar in f , h , 5 μm. g , i Quantitation of the transwell invasion described in f , h . j A representative image of tube formation assays of HUVEC and HDLMVEC after co-culture with A498 overexpressing AR. The HUVEC cells during coculture with A498 cells were also blocked with VEGF-A neutralizing antibody (R D Systems) at the concentration of 0.08 µg/ml. The endothelial cells were pre-stained with Calcein AM at the concentration of 2 µM and monitored with fluorescence microscope. l Tube formation assay of HUVEC and HDLMVEC after co-culture with SW839 cells with AR knockdown. The SW839 cells were infected with lentivirus for AR and sh-VEGF-C were used for co-culture with HDLMVEC cells for the rescue assay. Scale bar in j , l , 10 μm. k , m Quantitation of the tube number described in j , l . The functional assays were all replicated four times. * p

    Journal: Nature Communications

    Article Title: Androgen receptor increases hematogenous metastasis yet decreases lymphatic metastasis of renal cell carcinoma

    doi: 10.1038/s41467-017-00701-6

    Figure Lengend Snippet: AR of ccRCC cells influences angiogenesis and lymphoangiogenesis. a Outline of co-culture system. Endothelial cells (HUVEC and HDLMVEC) were co-cultured with ccRCC cells with indicated treatments (with/without overexpression/knockdown of AR) for 72 h, and then the endothelial cells were harvested. After harvest, the endothelial cells were maintained in mixed conditioned medium (CM from co-culture system) with fresh media at the ratio of 1:1. b , d Wound-healing assays of HUVEC and HDLMVEC cells co-cultured with A498 b and SW839 d with altered AR expression at indicated time points. Scale bar in b , d 10 μm. c , e Quantitation of endothelial cells migration in wound-healing assay after co-culture with indicated ccRCC cells as described in b , d . f , h Transwell invasion assay of HUVEC ( upper ) and HDLMVEC ( lower ) after co-culture with A498 cells overexpressing AR ( f ) and SW839 cells with AR knocked down ( h ). After 24 h, the invaded cells were stained and five random fields per well were analyzed. Scale bar in f , h , 5 μm. g , i Quantitation of the transwell invasion described in f , h . j A representative image of tube formation assays of HUVEC and HDLMVEC after co-culture with A498 overexpressing AR. The HUVEC cells during coculture with A498 cells were also blocked with VEGF-A neutralizing antibody (R D Systems) at the concentration of 0.08 µg/ml. The endothelial cells were pre-stained with Calcein AM at the concentration of 2 µM and monitored with fluorescence microscope. l Tube formation assay of HUVEC and HDLMVEC after co-culture with SW839 cells with AR knockdown. The SW839 cells were infected with lentivirus for AR and sh-VEGF-C were used for co-culture with HDLMVEC cells for the rescue assay. Scale bar in j , l , 10 μm. k , m Quantitation of the tube number described in j , l . The functional assays were all replicated four times. * p

    Article Snippet: HDLMVEC cells were purchased from Cell Applications, Inc. (San Diego, CA) and maintained with endothelial cell growth medium in attachment factor solution pre-coated flasks.

    Techniques: Co-Culture Assay, Cell Culture, Over Expression, Expressing, Quantitation Assay, Migration, Wound Healing Assay, Transwell Invasion Assay, Staining, Concentration Assay, Fluorescence, Microscopy, Tube Formation Assay, Infection, Rescue Assay, Functional Assay

    Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of GAPDH- and SR-BI-mRNA bands in negative control siRNA (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications

    Journal: Cellular and molecular life sciences : CMLS

    Article Title: Apolipoprotein CIII hyperactivates β cell CaV1 channels through SR-BI/β1 integrin-dependent coactivation of PKA and Src

    doi: 10.1007/s00018-013-1442-x

    Figure Lengend Snippet: Knockdown of SR-BI prevents apolipoprotein CIII-induced enhancement of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of GAPDH- and SR-BI-mRNA bands in negative control siRNA (NC siRNA)- and SR-BI siRNA-transfected cells. b Quantifications

    Article Snippet: Silencer Select Negative Control siRNA (4390843), not targeting any gene product, and Silencer Select GAPDH Positive Control siRNA (4390849), efficiently silencing GAPDH in human, mouse, and rat cells, were purchased from Applied Biosystems/Ambion (Austin, TX, USA).

    Techniques: Negative Control, Transfection

    Knockdown of β1 integrin abrogates apolipoprotein CIII-induced exaggeration of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of β1 integrin- and GAPDH-immunoreactive bands in β1 integrin siRNA #1-, negative control

    Journal: Cellular and molecular life sciences : CMLS

    Article Title: Apolipoprotein CIII hyperactivates β cell CaV1 channels through SR-BI/β1 integrin-dependent coactivation of PKA and Src

    doi: 10.1007/s00018-013-1442-x

    Figure Lengend Snippet: Knockdown of β1 integrin abrogates apolipoprotein CIII-induced exaggeration of whole-cell Ca 2+ currents in RINm5F cells. a Representative blots of β1 integrin- and GAPDH-immunoreactive bands in β1 integrin siRNA #1-, negative control

    Article Snippet: Silencer Select Negative Control siRNA (4390843), not targeting any gene product, and Silencer Select GAPDH Positive Control siRNA (4390849), efficiently silencing GAPDH in human, mouse, and rat cells, were purchased from Applied Biosystems/Ambion (Austin, TX, USA).

    Techniques: Negative Control