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  • 99
    Millipore cefotaxime
    Growth competition between E. coli MG1655(pC193) and E. coli MG1655(pV38-8) in the absence of antibiotic selective pressure (a), in the presence of 0.5 μg/ml <t>cefotaxime</t> (CTX) (b), and in the presence of 8 μg/ml cefotaxime (c). The data
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    Becton Dickinson cefotaxime
    Pulsed-field gel electrophoresis (PFGE) profiles and virulence characteristics of the 17 Shiga toxin-producing Escherichia coli (STEC) O91:H14 isolates with sequence type 33. The data, including multilocus sequence types, stx genotypes, reverse passive latex agglutination (RPLA) titers of Shiga toxins (Stxs), glutamate-induced acid resistance, and antibiotic resistance phenotypes, were combined and presented with the pulsotypes. The STEC isolates were divided into three pulsotypes (A to C) based on > 80% similarity of PFGE profiles. The RPLA titers of Stxs and the acid survival rates (AR2) were quantitated and compared with those of EDL933, which produced both Stx 1 and Stx 2 (1:16 and ≥ 1:128 RPLA titers, respectively) as well as surviving well in EG media (pH 2.5) with 1.5 mM glutamate. AM, ampicillin; AN, amikacin; CF, cephalothin; CIP, ciprofloxacin; S, streptomycin; CTX, <t>cefotaxime;</t> I, intermediate resistance. * p
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    Chong Kun Dang cefotaxime
    Time-kill curves for V. vulnificus CMCP6 after incubation with 3/4 MICs of <t>cefotaxime</t> alone, minocycline alone, ciprofloxacin alone, cefotaxime-plus-ciprofloxacin or cefotaxime-plus-minocycline. CFU, colony-forming unit; MIC, minimum inhibitory concentration.
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    Duchefa cefotaxime
    Transformation of cv.Pusa 9712 half seed explants by Agrobacterium tumefaciens strain EHA105 harboring binary vector pCAMBIA1300- p68 to enhance salinity stress tolerance. a mature dry seeds of soybean cv. Pusa 9712 used for preparing the half seed explants; b 1-day old imbibed seeds; c half seed explants made from imbibed seeds (black arrows direct the embryonic area); d explant after infection with A. tumefaciens EHA105 carrying pCAMBIA1300- p68 plasmid and co-cultivated for 5 days in the dark; e shoot induction from explant in SIM supplemented with <t>cefotaxime</t> (200 mg l − 1 ) without NaCl (after 15 days of culture); f , g selection of regenerated shoots in SIM containing cefotaxime (200 mg l − 1 ) and NaCl (75 mM) [after 30 days of culture]; h elongated shoots in SEM supplemented with cefotaxime (100 mg l − 1 ) and NaCl (75 mM) [after 30 days of culture]; i rooted shoots in RIM supplemented with cefotaxime (100 mg l − 1 ) and NaCl (50 mM) [after 30 days of culture]; j putatively transformed ( T o ) plants maintained in growth chamber; k acclimatization of survived ( T o ) plants under green house condition
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    Fresenius Kabi cefotaxim
    Transformation of cv.Pusa 9712 half seed explants by Agrobacterium tumefaciens strain EHA105 harboring binary vector pCAMBIA1300- p68 to enhance salinity stress tolerance. a mature dry seeds of soybean cv. Pusa 9712 used for preparing the half seed explants; b 1-day old imbibed seeds; c half seed explants made from imbibed seeds (black arrows direct the embryonic area); d explant after infection with A. tumefaciens EHA105 carrying pCAMBIA1300- p68 plasmid and co-cultivated for 5 days in the dark; e shoot induction from explant in SIM supplemented with <t>cefotaxime</t> (200 mg l − 1 ) without NaCl (after 15 days of culture); f , g selection of regenerated shoots in SIM containing cefotaxime (200 mg l − 1 ) and NaCl (75 mM) [after 30 days of culture]; h elongated shoots in SEM supplemented with cefotaxime (100 mg l − 1 ) and NaCl (75 mM) [after 30 days of culture]; i rooted shoots in RIM supplemented with cefotaxime (100 mg l − 1 ) and NaCl (50 mM) [after 30 days of culture]; j putatively transformed ( T o ) plants maintained in growth chamber; k acclimatization of survived ( T o ) plants under green house condition
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    HiMedia Laboratories cefotaxime
    Extended spectrum β-Lactamases producing K . pneumoniae (A = <t>Cefotaxime</t> B = Cefotaxime + Clavulanic acid) C = Ceftazidime D = Ceftazidime + Clavulanic acid) Clavulanic acid inhibited an extended spectrum of β-lactamases with the occurrence of a growth inhibition zone.
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    Sangon Biotech cefotaxime sodium
    Evaluation of the survival of specific bacteria, and the antibiotic preconditioning effects. a Schematic showing the experimental procedures for assessing the viability of E. coli during transplantation using STAMP. b Flow cytometry analysis of the transplanted E. coli strains K12 (left) and Nissle 1917 (right). The inserted numbers indicate the survival rates. c Statistical analysis of the survival rates of transplanted E. coli K12 and Nissle 1917. Mean ± s.d. are presented for n = 3. d Flow cytometry analysis of the STAMP-labeled gut microbiota from recipient mice with preconditioning of vancomycin, polymyxin B, <t>cefotaxime,</t> or metronidazole. The inserted numbers indicate the survival rates. e Statistical analysis of the survival rates of the transplanted microbiotas from recipient mice with antibiotic preconditioning. Mean ± s.d. are presented for n = 3
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    Applichem cefotaxime
    Evaluation of the survival of specific bacteria, and the antibiotic preconditioning effects. a Schematic showing the experimental procedures for assessing the viability of E. coli during transplantation using STAMP. b Flow cytometry analysis of the transplanted E. coli strains K12 (left) and Nissle 1917 (right). The inserted numbers indicate the survival rates. c Statistical analysis of the survival rates of transplanted E. coli K12 and Nissle 1917. Mean ± s.d. are presented for n = 3. d Flow cytometry analysis of the STAMP-labeled gut microbiota from recipient mice with preconditioning of vancomycin, polymyxin B, <t>cefotaxime,</t> or metronidazole. The inserted numbers indicate the survival rates. e Statistical analysis of the survival rates of the transplanted microbiotas from recipient mice with antibiotic preconditioning. Mean ± s.d. are presented for n = 3
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    Astral Scientific cefotaxime
    Evaluation of the survival of specific bacteria, and the antibiotic preconditioning effects. a Schematic showing the experimental procedures for assessing the viability of E. coli during transplantation using STAMP. b Flow cytometry analysis of the transplanted E. coli strains K12 (left) and Nissle 1917 (right). The inserted numbers indicate the survival rates. c Statistical analysis of the survival rates of transplanted E. coli K12 and Nissle 1917. Mean ± s.d. are presented for n = 3. d Flow cytometry analysis of the STAMP-labeled gut microbiota from recipient mice with preconditioning of vancomycin, polymyxin B, <t>cefotaxime,</t> or metronidazole. The inserted numbers indicate the survival rates. e Statistical analysis of the survival rates of the transplanted microbiotas from recipient mice with antibiotic preconditioning. Mean ± s.d. are presented for n = 3
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    91
    Hardy Diagnostics cefotaxime
    Concentration of target organisms from p-trap biofilms harvested from p-traps that had been in place at Hospital 2 in a patient room sink ( a ) and a HCP sink ( b ) for 1, 3, 6, 9, and 12 weeks. HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a <t>cefotaxime-containing</t> medium.
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    Sanofi cefotaxime
    Concentration of target organisms from p-trap biofilms harvested from p-traps that had been in place at Hospital 2 in a patient room sink ( a ) and a HCP sink ( b ) for 1, 3, 6, 9, and 12 weeks. HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a <t>cefotaxime-containing</t> medium.
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    Shimadzu Corporation cefotaxime
    Concentration of target organisms from p-trap biofilms harvested from p-traps that had been in place at Hospital 2 in a patient room sink ( a ) and a HCP sink ( b ) for 1, 3, 6, 9, and 12 weeks. HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a <t>cefotaxime-containing</t> medium.
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    Tokyo Chemical Industry cefotaxime
    Chemical structures of representative β-lactam substrates used in this study, as well as compound 6142342, used as an IMP inhibitor. For <t>cefotaxime,</t> the positions of the R1 and R2 groups are indicated exemplarily.
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    92
    Chugai cefotaxime
    Chemical structures of representative β-lactam substrates used in this study, as well as compound 6142342, used as an IMP inhibitor. For <t>cefotaxime,</t> the positions of the R1 and R2 groups are indicated exemplarily.
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    Glaxo Smith cefotaxime
    Chemical structures of representative β-lactam substrates used in this study, as well as compound 6142342, used as an IMP inhibitor. For <t>cefotaxime,</t> the positions of the R1 and R2 groups are indicated exemplarily.
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    Merck & Co cefotaxime
    Chemical structures of representative β-lactam substrates used in this study, as well as compound 6142342, used as an IMP inhibitor. For <t>cefotaxime,</t> the positions of the R1 and R2 groups are indicated exemplarily.
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    FUJIFILM cefotaxime
    Rates of resistance to 11 antimicrobials among Enterobacter isolates (n = 60) from companion animals. a AMP, ampicillin; ACV, amoxicillin-clavulanic acid; CMZ, cefmetazole; CTX, <t>cefotaxime;</t> CAZ, ceftazidime, MPM, meropenem; TET, tetracycline; GEN, gentamicin; CHL, chloramphenicol; TMS, trimethoprim/sulfamethoxazole; CIP, ciprofloxacin.
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    PhytoTechnology Laboratories cefotaxime
    Rates of resistance to 11 antimicrobials among Enterobacter isolates (n = 60) from companion animals. a AMP, ampicillin; ACV, amoxicillin-clavulanic acid; CMZ, cefmetazole; CTX, <t>cefotaxime;</t> CAZ, ceftazidime, MPM, meropenem; TET, tetracycline; GEN, gentamicin; CHL, chloramphenicol; TMS, trimethoprim/sulfamethoxazole; CIP, ciprofloxacin.
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    Research Products International cefotaxime
    Rates of resistance to 11 antimicrobials among Enterobacter isolates (n = 60) from companion animals. a AMP, ampicillin; ACV, amoxicillin-clavulanic acid; CMZ, cefmetazole; CTX, <t>cefotaxime;</t> CAZ, ceftazidime, MPM, meropenem; TET, tetracycline; GEN, gentamicin; CHL, chloramphenicol; TMS, trimethoprim/sulfamethoxazole; CIP, ciprofloxacin.
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    Aventis cefotaxime
    The active site of CTX-M-type β-lactamase with aztreonam (A and B), ceftazidime (C and D), or <t>cefotaxime</t> (E and F). (A, C, and E) CTX-M-18; (B, D, and F) CTX-M-19. Red broken lines indicate hydrogen bonds. Black thin double arrow indicates an interatomic distance longer than hydrogen bond. Blue broken double arrow indicates a steric interaction with the hydroxyl group of S167 and the thiazole ring.
    Cefotaxime, supplied by Aventis, used in various techniques. Bioz Stars score: 92/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Reig Jofre cefotaxime
    The active site of CTX-M-type β-lactamase with aztreonam (A and B), ceftazidime (C and D), or <t>cefotaxime</t> (E and F). (A, C, and E) CTX-M-18; (B, D, and F) CTX-M-19. Red broken lines indicate hydrogen bonds. Black thin double arrow indicates an interatomic distance longer than hydrogen bond. Blue broken double arrow indicates a steric interaction with the hydroxyl group of S167 and the thiazole ring.
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    EIPICO cefotaxime
    The active site of CTX-M-type β-lactamase with aztreonam (A and B), ceftazidime (C and D), or <t>cefotaxime</t> (E and F). (A, C, and E) CTX-M-18; (B, D, and F) CTX-M-19. Red broken lines indicate hydrogen bonds. Black thin double arrow indicates an interatomic distance longer than hydrogen bond. Blue broken double arrow indicates a steric interaction with the hydroxyl group of S167 and the thiazole ring.
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    Melford Laboratories cefotaxime
    The active site of CTX-M-type β-lactamase with aztreonam (A and B), ceftazidime (C and D), or <t>cefotaxime</t> (E and F). (A, C, and E) CTX-M-18; (B, D, and F) CTX-M-19. Red broken lines indicate hydrogen bonds. Black thin double arrow indicates an interatomic distance longer than hydrogen bond. Blue broken double arrow indicates a steric interaction with the hydroxyl group of S167 and the thiazole ring.
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    Nissui Pharmaceutical cefotaxime
    The active site of CTX-M-type β-lactamase with aztreonam (A and B), ceftazidime (C and D), or <t>cefotaxime</t> (E and F). (A, C, and E) CTX-M-18; (B, D, and F) CTX-M-19. Red broken lines indicate hydrogen bonds. Black thin double arrow indicates an interatomic distance longer than hydrogen bond. Blue broken double arrow indicates a steric interaction with the hydroxyl group of S167 and the thiazole ring.
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    Valiant cefotaxime
    The active site of CTX-M-type β-lactamase with aztreonam (A and B), ceftazidime (C and D), or <t>cefotaxime</t> (E and F). (A, C, and E) CTX-M-18; (B, D, and F) CTX-M-19. Red broken lines indicate hydrogen bonds. Black thin double arrow indicates an interatomic distance longer than hydrogen bond. Blue broken double arrow indicates a steric interaction with the hydroxyl group of S167 and the thiazole ring.
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    Image Search Results


    Growth competition between E. coli MG1655(pC193) and E. coli MG1655(pV38-8) in the absence of antibiotic selective pressure (a), in the presence of 0.5 μg/ml cefotaxime (CTX) (b), and in the presence of 8 μg/ml cefotaxime (c). The data

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Characterization of IncI1 Sequence Type 71 Epidemic Plasmid Lineage Responsible for the Recent Dissemination of CTX-M-65 Extended-Spectrum β-Lactamase in the Bolivian Chaco Region

    doi: 10.1128/AAC.00589-15

    Figure Lengend Snippet: Growth competition between E. coli MG1655(pC193) and E. coli MG1655(pV38-8) in the absence of antibiotic selective pressure (a), in the presence of 0.5 μg/ml cefotaxime (CTX) (b), and in the presence of 8 μg/ml cefotaxime (c). The data

    Article Snippet: Conjugal transfer of pC193 into the other enterobacterial hosts was investigated under the same experimental conditions, using E. coli MKD-135(pC193) as the donor and MacConkey agar (Oxoid) plus cefotaxime (10 μg/ml) and chloramphenicol (50 μg/ml) (Sigma-Aldrich) for selection of transconjugants.

    Techniques:

    Pulsed-field gel electrophoresis (PFGE) profiles and virulence characteristics of the 17 Shiga toxin-producing Escherichia coli (STEC) O91:H14 isolates with sequence type 33. The data, including multilocus sequence types, stx genotypes, reverse passive latex agglutination (RPLA) titers of Shiga toxins (Stxs), glutamate-induced acid resistance, and antibiotic resistance phenotypes, were combined and presented with the pulsotypes. The STEC isolates were divided into three pulsotypes (A to C) based on > 80% similarity of PFGE profiles. The RPLA titers of Stxs and the acid survival rates (AR2) were quantitated and compared with those of EDL933, which produced both Stx 1 and Stx 2 (1:16 and ≥ 1:128 RPLA titers, respectively) as well as surviving well in EG media (pH 2.5) with 1.5 mM glutamate. AM, ampicillin; AN, amikacin; CF, cephalothin; CIP, ciprofloxacin; S, streptomycin; CTX, cefotaxime; I, intermediate resistance. * p

    Journal: Journal of Veterinary Science

    Article Title: Molecular epidemiology of sequence type 33 of Shiga toxin-producing Escherichia coli O91:H14 isolates from human patients and retail meats in Korea

    doi: 10.4142/jvs.2019.20.1.87

    Figure Lengend Snippet: Pulsed-field gel electrophoresis (PFGE) profiles and virulence characteristics of the 17 Shiga toxin-producing Escherichia coli (STEC) O91:H14 isolates with sequence type 33. The data, including multilocus sequence types, stx genotypes, reverse passive latex agglutination (RPLA) titers of Shiga toxins (Stxs), glutamate-induced acid resistance, and antibiotic resistance phenotypes, were combined and presented with the pulsotypes. The STEC isolates were divided into three pulsotypes (A to C) based on > 80% similarity of PFGE profiles. The RPLA titers of Stxs and the acid survival rates (AR2) were quantitated and compared with those of EDL933, which produced both Stx 1 and Stx 2 (1:16 and ≥ 1:128 RPLA titers, respectively) as well as surviving well in EG media (pH 2.5) with 1.5 mM glutamate. AM, ampicillin; AN, amikacin; CF, cephalothin; CIP, ciprofloxacin; S, streptomycin; CTX, cefotaxime; I, intermediate resistance. * p

    Article Snippet: Using a standard disk diffusion method with interpretive criteria of the inhibitory zone diameters (mm) according to Clinical and Laboratory Standard Institute standards, we observed that all strains were susceptible or intermediate resistant to one or more antimicrobials, including ampicillin (10 µg; BD, USA), amikacin (30 µg; BD), cephalothin (30 µg; BD), ciprofloxacin (5 µg; BD), streptomycin (10 µg; BD), and cefotaxime (30 µg; BD) ( ).

    Techniques: Pulsed-Field Gel, Electrophoresis, Sequencing, Agglutination, Produced

    Time-kill curves for V. vulnificus CMCP6 after incubation with 3/4 MICs of cefotaxime alone, minocycline alone, ciprofloxacin alone, cefotaxime-plus-ciprofloxacin or cefotaxime-plus-minocycline. CFU, colony-forming unit; MIC, minimum inhibitory concentration.

    Journal: PLoS ONE

    Article Title: In Vivo Efficacy of the Combination of Ciprofloxacin and Cefotaxime against Vibrio vulnificus Sepsis

    doi: 10.1371/journal.pone.0101118

    Figure Lengend Snippet: Time-kill curves for V. vulnificus CMCP6 after incubation with 3/4 MICs of cefotaxime alone, minocycline alone, ciprofloxacin alone, cefotaxime-plus-ciprofloxacin or cefotaxime-plus-minocycline. CFU, colony-forming unit; MIC, minimum inhibitory concentration.

    Article Snippet: Cefotaxime (Chong Kun Dang Pharmaceutical, Seoul, Republic of Korea), minocycline (Sigma-Aldrich, St. Louis, MO) and ciprofloxacin (Ildong Pharmaceutical, Seoul, Republic of Korea) were used throughout the study.

    Techniques: Incubation, Concentration Assay

    Survival rates of mice in each treatment group inoculated with 1×10 8 cfu V. vulnificus . The 96-h survival rate of the cefotaxime-plus-ciprofloxacin group (85%, 17/20) was significantly higher than that of the cefotaxime (0%, 0/20) or the cefotaxime-plus-minocycline groups (35%, 7/20) ( P

    Journal: PLoS ONE

    Article Title: In Vivo Efficacy of the Combination of Ciprofloxacin and Cefotaxime against Vibrio vulnificus Sepsis

    doi: 10.1371/journal.pone.0101118

    Figure Lengend Snippet: Survival rates of mice in each treatment group inoculated with 1×10 8 cfu V. vulnificus . The 96-h survival rate of the cefotaxime-plus-ciprofloxacin group (85%, 17/20) was significantly higher than that of the cefotaxime (0%, 0/20) or the cefotaxime-plus-minocycline groups (35%, 7/20) ( P

    Article Snippet: Cefotaxime (Chong Kun Dang Pharmaceutical, Seoul, Republic of Korea), minocycline (Sigma-Aldrich, St. Louis, MO) and ciprofloxacin (Ildong Pharmaceutical, Seoul, Republic of Korea) were used throughout the study.

    Techniques: Mouse Assay

    The effects of sub-inhibitory concentrations of antibiotics on V. vulnificus cytotoxicity and rtxA1 transcription. A. Cytotoxicity assay. The impaired cytotoxicity of ΔrtxA1 compared with that of the WT strain shows that cytotoxicity at 120 min is due principally to RtxA1. V. vulnificus cytotoxicity is inhibited more markedly by 1/4 MIC of ciprofloxacin than by 1/4 MICs of cefotaxime or minocycline (n = 12 per group). B. Transcriptional reporter assay. The transcription of rtxA1 is more efficiently inhibited by 1/4 MIC of ciprofloxacin than by 1/4 MICs of cefotaxime or minocycline (n = 4 per group). WT, MO6-24/O; ΔrtxA1 , CMM770 (MO6-24/O background with a deletion mutation in the rtxA1 gene); CTX, cefotaxime; CIP, ciprofloxacin; MCL, minocycline. * P

    Journal: PLoS ONE

    Article Title: In Vivo Efficacy of the Combination of Ciprofloxacin and Cefotaxime against Vibrio vulnificus Sepsis

    doi: 10.1371/journal.pone.0101118

    Figure Lengend Snippet: The effects of sub-inhibitory concentrations of antibiotics on V. vulnificus cytotoxicity and rtxA1 transcription. A. Cytotoxicity assay. The impaired cytotoxicity of ΔrtxA1 compared with that of the WT strain shows that cytotoxicity at 120 min is due principally to RtxA1. V. vulnificus cytotoxicity is inhibited more markedly by 1/4 MIC of ciprofloxacin than by 1/4 MICs of cefotaxime or minocycline (n = 12 per group). B. Transcriptional reporter assay. The transcription of rtxA1 is more efficiently inhibited by 1/4 MIC of ciprofloxacin than by 1/4 MICs of cefotaxime or minocycline (n = 4 per group). WT, MO6-24/O; ΔrtxA1 , CMM770 (MO6-24/O background with a deletion mutation in the rtxA1 gene); CTX, cefotaxime; CIP, ciprofloxacin; MCL, minocycline. * P

    Article Snippet: Cefotaxime (Chong Kun Dang Pharmaceutical, Seoul, Republic of Korea), minocycline (Sigma-Aldrich, St. Louis, MO) and ciprofloxacin (Ildong Pharmaceutical, Seoul, Republic of Korea) were used throughout the study.

    Techniques: Cytotoxicity Assay, Reporter Assay, Mutagenesis

    Transformation of cv.Pusa 9712 half seed explants by Agrobacterium tumefaciens strain EHA105 harboring binary vector pCAMBIA1300- p68 to enhance salinity stress tolerance. a mature dry seeds of soybean cv. Pusa 9712 used for preparing the half seed explants; b 1-day old imbibed seeds; c half seed explants made from imbibed seeds (black arrows direct the embryonic area); d explant after infection with A. tumefaciens EHA105 carrying pCAMBIA1300- p68 plasmid and co-cultivated for 5 days in the dark; e shoot induction from explant in SIM supplemented with cefotaxime (200 mg l − 1 ) without NaCl (after 15 days of culture); f , g selection of regenerated shoots in SIM containing cefotaxime (200 mg l − 1 ) and NaCl (75 mM) [after 30 days of culture]; h elongated shoots in SEM supplemented with cefotaxime (100 mg l − 1 ) and NaCl (75 mM) [after 30 days of culture]; i rooted shoots in RIM supplemented with cefotaxime (100 mg l − 1 ) and NaCl (50 mM) [after 30 days of culture]; j putatively transformed ( T o ) plants maintained in growth chamber; k acclimatization of survived ( T o ) plants under green house condition

    Journal: 3 Biotech

    Article Title: Pea p68, a DEAD-box helicase, enhances salt tolerance in marker-free transgenic plants of soybean [Glycine max (L.) Merrill]

    doi: 10.1007/s13205-018-1553-z

    Figure Lengend Snippet: Transformation of cv.Pusa 9712 half seed explants by Agrobacterium tumefaciens strain EHA105 harboring binary vector pCAMBIA1300- p68 to enhance salinity stress tolerance. a mature dry seeds of soybean cv. Pusa 9712 used for preparing the half seed explants; b 1-day old imbibed seeds; c half seed explants made from imbibed seeds (black arrows direct the embryonic area); d explant after infection with A. tumefaciens EHA105 carrying pCAMBIA1300- p68 plasmid and co-cultivated for 5 days in the dark; e shoot induction from explant in SIM supplemented with cefotaxime (200 mg l − 1 ) without NaCl (after 15 days of culture); f , g selection of regenerated shoots in SIM containing cefotaxime (200 mg l − 1 ) and NaCl (75 mM) [after 30 days of culture]; h elongated shoots in SEM supplemented with cefotaxime (100 mg l − 1 ) and NaCl (75 mM) [after 30 days of culture]; i rooted shoots in RIM supplemented with cefotaxime (100 mg l − 1 ) and NaCl (50 mM) [after 30 days of culture]; j putatively transformed ( T o ) plants maintained in growth chamber; k acclimatization of survived ( T o ) plants under green house condition

    Article Snippet: After 5 days of co-cultivation with EHA105 carrying pCAMBIA 1300- p68 , the explants were washed, blot dried and inoculated on SIM supplemented with 200 mg l− 1 cefotaxime (Duchefa, Haarlem, Netherlands), for initiation of shoot buds.

    Techniques: Transformation Assay, Plasmid Preparation, Infection, Selection

    Extended spectrum β-Lactamases producing K . pneumoniae (A = Cefotaxime B = Cefotaxime + Clavulanic acid) C = Ceftazidime D = Ceftazidime + Clavulanic acid) Clavulanic acid inhibited an extended spectrum of β-lactamases with the occurrence of a growth inhibition zone.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Characterization of AmpC, CTX-M and MBLs types of β-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli producing Extended Spectrum β-lactamases in Kerman, Iran

    doi: 10.5812/jjm.8756

    Figure Lengend Snippet: Extended spectrum β-Lactamases producing K . pneumoniae (A = Cefotaxime B = Cefotaxime + Clavulanic acid) C = Ceftazidime D = Ceftazidime + Clavulanic acid) Clavulanic acid inhibited an extended spectrum of β-lactamases with the occurrence of a growth inhibition zone.

    Article Snippet: The antibiotics tested were ceftizoxime (30 μg), cefotaxime (30 μg), ceftazidime (30 μg), cephalexin (30 μg), amoxicillin (30 μg), imipenem (10 μg), cefepime (30 μg), cefoxitin (30 μg), gentamycin (30 μg), tetracycline (30 μg), trimethoprim/sulfamethoxazole (30 μg), nalidixic acid and ciprofloxacin (30 μg) (Himedia, India).

    Techniques: Inhibition

    Evaluation of the survival of specific bacteria, and the antibiotic preconditioning effects. a Schematic showing the experimental procedures for assessing the viability of E. coli during transplantation using STAMP. b Flow cytometry analysis of the transplanted E. coli strains K12 (left) and Nissle 1917 (right). The inserted numbers indicate the survival rates. c Statistical analysis of the survival rates of transplanted E. coli K12 and Nissle 1917. Mean ± s.d. are presented for n = 3. d Flow cytometry analysis of the STAMP-labeled gut microbiota from recipient mice with preconditioning of vancomycin, polymyxin B, cefotaxime, or metronidazole. The inserted numbers indicate the survival rates. e Statistical analysis of the survival rates of the transplanted microbiotas from recipient mice with antibiotic preconditioning. Mean ± s.d. are presented for n = 3

    Journal: Nature Communications

    Article Title: Assessing the viability of transplanted gut microbiota by sequential tagging with D-amino acid-based metabolic probes

    doi: 10.1038/s41467-019-09267-x

    Figure Lengend Snippet: Evaluation of the survival of specific bacteria, and the antibiotic preconditioning effects. a Schematic showing the experimental procedures for assessing the viability of E. coli during transplantation using STAMP. b Flow cytometry analysis of the transplanted E. coli strains K12 (left) and Nissle 1917 (right). The inserted numbers indicate the survival rates. c Statistical analysis of the survival rates of transplanted E. coli K12 and Nissle 1917. Mean ± s.d. are presented for n = 3. d Flow cytometry analysis of the STAMP-labeled gut microbiota from recipient mice with preconditioning of vancomycin, polymyxin B, cefotaxime, or metronidazole. The inserted numbers indicate the survival rates. e Statistical analysis of the survival rates of the transplanted microbiotas from recipient mice with antibiotic preconditioning. Mean ± s.d. are presented for n = 3

    Article Snippet: Vancomycin hydrochloride, metronidazole, polymyxin B sulfate, and cefotaxime sodium were bought from Sangon Biotech (Shanghai, China).

    Techniques: Transplantation Assay, Flow Cytometry, Cytometry, Labeling, Mouse Assay

    Concentration of target organisms from p-trap biofilms harvested from p-traps that had been in place at Hospital 2 in a patient room sink ( a ) and a HCP sink ( b ) for 1, 3, 6, 9, and 12 weeks. HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a cefotaxime-containing medium.

    Journal: Scientific Reports

    Article Title: A microbiological survey of handwashing sinks in the hospital built environment reveals differences in patient room and healthcare personnel sinks

    doi: 10.1038/s41598-020-65052-7

    Figure Lengend Snippet: Concentration of target organisms from p-trap biofilms harvested from p-traps that had been in place at Hospital 2 in a patient room sink ( a ) and a HCP sink ( b ) for 1, 3, 6, 9, and 12 weeks. HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a cefotaxime-containing medium.

    Article Snippet: Culture conditionsAll of the samples above, excluding the tap water, were plated on R2A, Middlebrook 7H11 (Remel, Lenexa, KS), Pseudosel (Becton Dickinson, Franklin Lakes, NJ), Chromagar KPC (Chromagar, Paris, France), and MacConkey with 2 mg/L cefotaxime (Hardy Diagnostics, Santa Maria, CA).

    Techniques: Concentration Assay

    Concentration of target organisms from 4 times weekly p-trap water samples from one patient room sink ( a ) and one HCP sink ( b ) over the six-week sample period. HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a cefotaxime-containing medium.

    Journal: Scientific Reports

    Article Title: A microbiological survey of handwashing sinks in the hospital built environment reveals differences in patient room and healthcare personnel sinks

    doi: 10.1038/s41598-020-65052-7

    Figure Lengend Snippet: Concentration of target organisms from 4 times weekly p-trap water samples from one patient room sink ( a ) and one HCP sink ( b ) over the six-week sample period. HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a cefotaxime-containing medium.

    Article Snippet: Culture conditionsAll of the samples above, excluding the tap water, were plated on R2A, Middlebrook 7H11 (Remel, Lenexa, KS), Pseudosel (Becton Dickinson, Franklin Lakes, NJ), Chromagar KPC (Chromagar, Paris, France), and MacConkey with 2 mg/L cefotaxime (Hardy Diagnostics, Santa Maria, CA).

    Techniques: Concentration Assay

    Concentration of target organisms in p-trap water pre- and post- p-trap exchange for sterilized p-trap in patient room ( a ) and HCP sinks ( b ). HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a cefotaxime-containing medium.

    Journal: Scientific Reports

    Article Title: A microbiological survey of handwashing sinks in the hospital built environment reveals differences in patient room and healthcare personnel sinks

    doi: 10.1038/s41598-020-65052-7

    Figure Lengend Snippet: Concentration of target organisms in p-trap water pre- and post- p-trap exchange for sterilized p-trap in patient room ( a ) and HCP sinks ( b ). HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a cefotaxime-containing medium.

    Article Snippet: Culture conditionsAll of the samples above, excluding the tap water, were plated on R2A, Middlebrook 7H11 (Remel, Lenexa, KS), Pseudosel (Becton Dickinson, Franklin Lakes, NJ), Chromagar KPC (Chromagar, Paris, France), and MacConkey with 2 mg/L cefotaxime (Hardy Diagnostics, Santa Maria, CA).

    Techniques: Concentration Assay

    Distribution of target organisms in a patient room sink and HCP sink at Hospital 2. Patient room T 1 , T 2 , and T 3 and HCP sink T 1 , T 2 , and T 3 represent the same sinks sampled at week 0 (T 1 ), week 3 (T 2 ) and week 6 (T 3 ). HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a cefotaxime-containing medium.

    Journal: Scientific Reports

    Article Title: A microbiological survey of handwashing sinks in the hospital built environment reveals differences in patient room and healthcare personnel sinks

    doi: 10.1038/s41598-020-65052-7

    Figure Lengend Snippet: Distribution of target organisms in a patient room sink and HCP sink at Hospital 2. Patient room T 1 , T 2 , and T 3 and HCP sink T 1 , T 2 , and T 3 represent the same sinks sampled at week 0 (T 1 ), week 3 (T 2 ) and week 6 (T 3 ). HPC – heterotrophic plate count, CRE – carbapenem-resistant Enterobacteriaceae, PA – Pseudomonas aeruginosa , OPP-C – opportunistic pathogens capable of growth on a cefotaxime-containing medium.

    Article Snippet: Culture conditionsAll of the samples above, excluding the tap water, were plated on R2A, Middlebrook 7H11 (Remel, Lenexa, KS), Pseudosel (Becton Dickinson, Franklin Lakes, NJ), Chromagar KPC (Chromagar, Paris, France), and MacConkey with 2 mg/L cefotaxime (Hardy Diagnostics, Santa Maria, CA).

    Techniques:

    Percentage of Enterobacteriaceae and P. aeruginosa isolates that are resistant (R) or have intermediate (I) resistance to the antibiotics tested. Isolates originating from biofilm (BF, p-trap and tail pipe) were compared to those from planktonic (P, p-trap water) samples ( a ) and isolates originating from HCP sinks were compared to those from patient room sinks ( b ). ATM – aztreonam, CAZ – ceftazidime, MEM – meropenem, ERT – ertapenem, CTX – cefotaxime.

    Journal: Scientific Reports

    Article Title: A microbiological survey of handwashing sinks in the hospital built environment reveals differences in patient room and healthcare personnel sinks

    doi: 10.1038/s41598-020-65052-7

    Figure Lengend Snippet: Percentage of Enterobacteriaceae and P. aeruginosa isolates that are resistant (R) or have intermediate (I) resistance to the antibiotics tested. Isolates originating from biofilm (BF, p-trap and tail pipe) were compared to those from planktonic (P, p-trap water) samples ( a ) and isolates originating from HCP sinks were compared to those from patient room sinks ( b ). ATM – aztreonam, CAZ – ceftazidime, MEM – meropenem, ERT – ertapenem, CTX – cefotaxime.

    Article Snippet: Culture conditionsAll of the samples above, excluding the tap water, were plated on R2A, Middlebrook 7H11 (Remel, Lenexa, KS), Pseudosel (Becton Dickinson, Franklin Lakes, NJ), Chromagar KPC (Chromagar, Paris, France), and MacConkey with 2 mg/L cefotaxime (Hardy Diagnostics, Santa Maria, CA).

    Techniques:

    Chemical structures of representative β-lactam substrates used in this study, as well as compound 6142342, used as an IMP inhibitor. For cefotaxime, the positions of the R1 and R2 groups are indicated exemplarily.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Elucidating the Role of Residue 67 in IMP-Type Metallo-β-Lactamase Evolution

    doi: 10.1128/AAC.01651-15

    Figure Lengend Snippet: Chemical structures of representative β-lactam substrates used in this study, as well as compound 6142342, used as an IMP inhibitor. For cefotaxime, the positions of the R1 and R2 groups are indicated exemplarily.

    Article Snippet: Cefotaxime was purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan), cefoxitin from Biosynth (Itaska, IL), chromacef from Sopharmia (St. Joseph, MO), and kanamycin from Fisher Scientific (Fair Lawn, NJ).

    Techniques:

    Rates of resistance to 11 antimicrobials among Enterobacter isolates (n = 60) from companion animals. a AMP, ampicillin; ACV, amoxicillin-clavulanic acid; CMZ, cefmetazole; CTX, cefotaxime; CAZ, ceftazidime, MPM, meropenem; TET, tetracycline; GEN, gentamicin; CHL, chloramphenicol; TMS, trimethoprim/sulfamethoxazole; CIP, ciprofloxacin.

    Journal: PLoS ONE

    Article Title: Phenotypic and molecular characterization of antimicrobial resistance in Enterobacter spp. isolates from companion animals in Japan

    doi: 10.1371/journal.pone.0174178

    Figure Lengend Snippet: Rates of resistance to 11 antimicrobials among Enterobacter isolates (n = 60) from companion animals. a AMP, ampicillin; ACV, amoxicillin-clavulanic acid; CMZ, cefmetazole; CTX, cefotaxime; CAZ, ceftazidime, MPM, meropenem; TET, tetracycline; GEN, gentamicin; CHL, chloramphenicol; TMS, trimethoprim/sulfamethoxazole; CIP, ciprofloxacin.

    Article Snippet: LLC., Tokyo, Japan), cefmetazole (CMZ, Sigma-Aldrich), cefotaxime (CTX, Wako Pure Chemical), ceftazidime (CAZ, Sigma-Aldrich), meropenem (MPM, Wako Pure Chemical), tetracycline (TET, Wako Pure Chemical), gentamicin (GEN, Sigma-Aldrich), chloramphenicol (CHL, Wako Pure Chemical), trimethoprim/sulfamethoxazole (TMS, Wako Pure Chemical), and ciprofloxacin (CIP, Wako Pure Chemical) were determined.

    Techniques:

    The active site of CTX-M-type β-lactamase with aztreonam (A and B), ceftazidime (C and D), or cefotaxime (E and F). (A, C, and E) CTX-M-18; (B, D, and F) CTX-M-19. Red broken lines indicate hydrogen bonds. Black thin double arrow indicates an interatomic distance longer than hydrogen bond. Blue broken double arrow indicates a steric interaction with the hydroxyl group of S167 and the thiazole ring.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Role of a Mutation at Position 167 of CTX-M-19 in Ceftazidime Hydrolysis

    doi: 10.1128/AAC.48.5.1454-1460.2004

    Figure Lengend Snippet: The active site of CTX-M-type β-lactamase with aztreonam (A and B), ceftazidime (C and D), or cefotaxime (E and F). (A, C, and E) CTX-M-18; (B, D, and F) CTX-M-19. Red broken lines indicate hydrogen bonds. Black thin double arrow indicates an interatomic distance longer than hydrogen bond. Blue broken double arrow indicates a steric interaction with the hydroxyl group of S167 and the thiazole ring.

    Article Snippet: Reference powders of the following antibiotics were kindly provided by their manufacturers: piperacillin (Toyama Chemical Co., Ltd., Tokyo, Japan), cefoxitin and imipenem (Banyu Pharmaceutical Co., Ltd., Tokyo, Japan), cefepime (Bristol Pharmaceutical Co., Tokyo, Japan), ceftizoxime (Fujisawa Pharmaceutical Co., Ltd., Tokyo, Japan), clavulanate and ceftazidime (Glaxo Smith Kline, Tokyo, Japan), cefotaxime (Aventis Pharma, Tokyo, Japan), aztreonam (Eisai Co., Ltd., Tokyo, Japan), and faropenem (Suntory, Ltd., Tokyo, Japan).

    Techniques: