cdna template Takara Search Results


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  • 99
    Thermo Fisher trizol reagent
    Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 635164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primescript rt reagent kit
    Primescript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 74091 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa sybr premix ex taq
    Sybr Premix Ex Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 46999 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr premix ex taq ii
    Sybr Premix Ex Taq Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 32368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher total rna
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 471882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa gdna eraser
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Gdna Eraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 27501 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcr amplification
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Pcr Amplification, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 21524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa taq dna polymerase
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 13199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa ex taq dna polymerase
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Ex Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 9323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa smart race cdna amplification kit
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Smart Race Cdna Amplification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 9386 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primescript 1st strand cdna synthesis kit
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Primescript 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 7752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher revertaid first strand cdna synthesis kit
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Revertaid First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 40122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa dntps
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Dntps, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa m mlv reverse transcriptase
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    M Mlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher first strand cdna
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    First Strand Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 84046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rna
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 177224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher steponeplus real time pcr system
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    Steponeplus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 52235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7500 real time pcr system
    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total <t>RNA</t> was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL <t>2000</t> plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.
    7500 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 54115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total RNA was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL 2000 plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.

    Journal: Applied and Environmental Microbiology

    Article Title: Efflux Transporter ArsK Is Responsible for Bacterial Resistance to Arsenite, Antimonite, Trivalent Roxarsone, and Methylarsenite

    doi: 10.1128/AEM.01842-18

    Figure Lengend Snippet: ArsR2 negatively regulates the expression of arsK . (A) RT-PCR analysis illustrating the cotranscription of arsR2 and arsK . Total RNA was extracted from strain GW4 grown in MMNH 4 medium with 1 mM As(III). M, molecular weight marker (DL 2000 plus). In lanes 1 and 4, total DNA was used as the template; in lanes 2 and 5, total RNA was used as the template; in lines 3 and 6, double-distilled water (ddH 2 O) was used as the template. The horizontal lines represent the locations of primers. (B) EMSAs. FAM-labeled arsR2 - arsK probe interacted with ArsR2 protein. The amounts of DNA probes and ArsR2 are shown in the tables above each panel. Competition EMSAs including arsR2 - arsK probe and ArsR2 protein with increasing amounts of As(III) (C), Sb(III) (D), Rox(III) (E), MAs(III) (F), As(V) (G), or dimethyl-As(III) (H). The amount of DNA probe was 100 ng, the amount of ArsR2 was 1.0 μM, and the amounts of each metalloid are shown in the tables above each panel.

    Article Snippet: Total RNA was extracted with TRIzol (Invitrogen) and incubated with RNase-free DNase I (TaKaRa) at 37°C to remove genomic DNA, and the reaction was then terminated by the addition of 50 mM EDTA at 65°C for 10 min. After determining the concentration of RNA by spectrophotometry (NanoDrop 2000; Thermo), 1,000 ng total RNA was reverse transcribed into cDNA with a RevertAid First Strand cDNA synthesis kit (Thermo).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Marker, Labeling