cdna library construction mrna Search Results


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  • 99
    New England Biolabs nebnext mrna library prep master mix for illumina
    Nebnext Mrna Library Prep Master Mix For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext mrna library prep master mix for illumina/product/New England Biolabs
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    Thermo Fisher dynabeads mrna direct kit
    Dynabeads Mrna Direct Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore mrna
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Mrna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2985 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa in fusion smarter cdna library construction kit
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    In Fusion Smarter Cdna Library Construction Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson smart cdna library construction kit
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Smart Cdna Library Construction Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc cdna library construction
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Cdna Library Construction, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 95/100, based on 5543 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa human spleen mrna
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Human Spleen Mrna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc cdna library generation kit mrna seq
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Cdna Library Generation Kit Mrna Seq, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore messenger rna standard isolation kit
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Messenger Rna Standard Isolation Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene messenger rna isolation kit
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Messenger Rna Isolation Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Stratagene mrna
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Mrna, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 2357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa poly a rna
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Poly A Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare poly a rna
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Poly A Rna, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 502 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs cdna library
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Cdna Library, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene creator smart cdna library construction
    The results of display of the <t>cDNA</t> library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified <t>mRNA</t> of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.
    Creator Smart Cdna Library Construction, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc library preparation pigment cell cdna library construction
    Schematic of <t>cDNA</t> library preparation. PolyA-selected <t>mRNA</t> (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.
    Library Preparation Pigment Cell Cdna Library Construction, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Illumina Inc sequencing messenger rna
    Schematic of <t>cDNA</t> library preparation. PolyA-selected <t>mRNA</t> (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.
    Sequencing Messenger Rna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen oligotex mrna kit
    Schematic of <t>cDNA</t> library preparation. PolyA-selected <t>mRNA</t> (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.
    Oligotex Mrna Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1950 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen oligotex mrna kits
    Schematic of <t>cDNA</t> library preparation. PolyA-selected <t>mRNA</t> (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.
    Oligotex Mrna Kits, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene mrna purification kit
    Schematic of <t>cDNA</t> library preparation. PolyA-selected <t>mRNA</t> (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.
    Mrna Purification Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche kapa mrna hyperprep rna seq library construction kit kapa roche
    Schematic of <t>cDNA</t> library preparation. PolyA-selected <t>mRNA</t> (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.
    Kapa Mrna Hyperprep Rna Seq Library Construction Kit Kapa Roche, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Illumina Inc mrna
    Schematic of <t>cDNA</t> library preparation. PolyA-selected <t>mRNA</t> (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.
    Mrna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 10125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The results of display of the cDNA library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified mRNA of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.

    Journal: Virology Journal

    Article Title: Identification of NCAM that interacts with the PHE-CoV spike protein

    doi: 10.1186/1743-422X-7-254

    Figure Lengend Snippet: The results of display of the cDNA library from N2a cells on T7 phage . (A) Lane1:The result of extracted total RNA of N2a cells. The electrophoresis results show 28 S and 18 S bands were clear, indicating the total RNA extraction without degradation. M: DL2000 Marker. (B) Lane1:The result of purified mRNA of N2a cells. OD260/OD280 = 1.950. The data show that the purified mRNA could be used for cDNA synthesis. M: DL2000 Marker. (C) Lane1 to 10: The PCR identified result of randomly picked phage clones of the library. Amplification of inserts in randomly selected clones revealed that the library contained > 90% recombinants with average insert size of > 300 bp. M: DL2000 Marker.

    Article Snippet: A cDNA library was constructed with 10 μg mRNA, following the manufacturer's instructions for the OrientExpress Random Primer cDNA Synthesis kit (Novagen, Madison, WI), with some modifications.

    Techniques: cDNA Library Assay, Electrophoresis, RNA Extraction, Marker, Purification, Polymerase Chain Reaction, Clone Assay, Amplification

    Schematic of cDNA library preparation. PolyA-selected mRNA (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.

    Journal: PLoS ONE

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin

    doi: 10.1371/journal.pone.0067801

    Figure Lengend Snippet: Schematic of cDNA library preparation. PolyA-selected mRNA (in red) is reverse transcribed using a polyT primer tailed with a universal primer (A). See Table S3 for primer sequences. MMLV reverse transcriptase adds cytosines to the 3′ end of the 1st strand cDNA (in black), allowing for template switching and addition of the 3′ universal primer (B). PCR amplification of the library is followed by RsaI and AluI enzymatic digestion of cDNAs (C), followed by the standard Illumina library preparation steps of end-repair, a single adenine addition, Y-adapter ligation (D), PCR enrichment, and size selection (mock gel shown in E with yellow box indicating area of gel removed for DNA extraction), prior to flowcell generation and sequencing.

    Article Snippet: mRNA Extraction, cDNA Synthesis, and Illumina Library Preparation Pigment cell cDNA library construction was as follows.

    Techniques: cDNA Library Assay, Polymerase Chain Reaction, Amplification, Ligation, Selection, DNA Extraction, Sequencing