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  • 99
    Thermo Fisher cdna levels
    Cdna Levels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad complementary dna levels
    Complementary Dna Levels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc cdna level
    Validation by quantitative on-line RT-PCR analyses of the differential expression between sweetpotato initiating storage and fibrous roots revealed by the use of <t>Illumina-based</t> sequencing at the <t>cDNA</t> level. Expression levels were measured in initiating storage root (ISR) and fibrous root (FR) samples of Georgia Jet sweetpotato variety, using at least four biological replicates. Each replicate consisted of cDNA representing pooled root tissue from 30 plants. Quantitative RT-PCR was performed and values were normalized relative to the expression levels of 18S rRNA in the same cDNA sample. Expression data are the means (± SE) of at least four replicates and are presented as relative expression values of the respective gene in the ISR sample relative to its expression in the FR sample (ISR/FR). The Y axis has a logarithmic scale. ADP glucose pyrophosphorylase – AGPase; coumaroyl-CoA synthase – 4CL; caffeoyl-CoA O-methyltransferase – CCoAOMT; cinnamyl alcohol dehydrogenase – CAD.
    Cdna Level, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega cdna level
    Validation by quantitative on-line RT-PCR analyses of the differential expression between sweetpotato initiating storage and fibrous roots revealed by the use of <t>Illumina-based</t> sequencing at the <t>cDNA</t> level. Expression levels were measured in initiating storage root (ISR) and fibrous root (FR) samples of Georgia Jet sweetpotato variety, using at least four biological replicates. Each replicate consisted of cDNA representing pooled root tissue from 30 plants. Quantitative RT-PCR was performed and values were normalized relative to the expression levels of 18S rRNA in the same cDNA sample. Expression data are the means (± SE) of at least four replicates and are presented as relative expression values of the respective gene in the ISR sample relative to its expression in the FR sample (ISR/FR). The Y axis has a logarithmic scale. ADP glucose pyrophosphorylase – AGPase; coumaroyl-CoA synthase – 4CL; caffeoyl-CoA O-methyltransferase – CCoAOMT; cinnamyl alcohol dehydrogenase – CAD.
    Cdna Level, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Unigene level cdna coverage
    Validation by quantitative on-line RT-PCR analyses of the differential expression between sweetpotato initiating storage and fibrous roots revealed by the use of <t>Illumina-based</t> sequencing at the <t>cDNA</t> level. Expression levels were measured in initiating storage root (ISR) and fibrous root (FR) samples of Georgia Jet sweetpotato variety, using at least four biological replicates. Each replicate consisted of cDNA representing pooled root tissue from 30 plants. Quantitative RT-PCR was performed and values were normalized relative to the expression levels of 18S rRNA in the same cDNA sample. Expression data are the means (± SE) of at least four replicates and are presented as relative expression values of the respective gene in the ISR sample relative to its expression in the FR sample (ISR/FR). The Y axis has a logarithmic scale. ADP glucose pyrophosphorylase – AGPase; coumaroyl-CoA synthase – 4CL; caffeoyl-CoA O-methyltransferase – CCoAOMT; cinnamyl alcohol dehydrogenase – CAD.
    Level Cdna Coverage, supplied by Unigene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mrna levels
    Validation by quantitative on-line RT-PCR analyses of the differential expression between sweetpotato initiating storage and fibrous roots revealed by the use of <t>Illumina-based</t> sequencing at the <t>cDNA</t> level. Expression levels were measured in initiating storage root (ISR) and fibrous root (FR) samples of Georgia Jet sweetpotato variety, using at least four biological replicates. Each replicate consisted of cDNA representing pooled root tissue from 30 plants. Quantitative RT-PCR was performed and values were normalized relative to the expression levels of 18S rRNA in the same cDNA sample. Expression data are the means (± SE) of at least four replicates and are presented as relative expression values of the respective gene in the ISR sample relative to its expression in the FR sample (ISR/FR). The Y axis has a logarithmic scale. ADP glucose pyrophosphorylase – AGPase; coumaroyl-CoA synthase – 4CL; caffeoyl-CoA O-methyltransferase – CCoAOMT; cinnamyl alcohol dehydrogenase – CAD.
    Mrna Levels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher exon level cdna affymetrix microarray
    Relative mRNA abundance between B16F0 exosomes and cells were consistent between qRT-PCR and <t>microarray</t> analyses (a) The abundance of 10 genes (Kpnb1, Rnf14, Rnd2, Ptp4a3, Ptpn11, Eif2c2, Hipk2, Eif4ebp2, Dnmt3a, and Wsb2) in B16F0 exosomes versus B16F0 cells were quantified by quantitative RT-PCR (mean ± s.d., N = 3). The qRT-PCR results were normalized to the average differential abundance of three control genes: Kpnb1, Rnf14, and Rnd2. (b) The relative abundances of mRNAs assayed by qRT-PCR were compared against the relative abundances of mRNAs assayed by <t>cDNA</t> microarray. The dotted line indicates that the two different assays provide the same results for relative abundance. (c) Full-length coding sequences (ORFs) were amplified by semi-quantitative RT-PCR. Equal concentrations of RNA were reverse-transcribed into cDNA and amplified by PCR. After 25 cycles, full-length open-reading frame amplicons were monitored every three cycles and resolved on agarose gel before the amplification was saturated.
    Exon Level Cdna Affymetrix Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad quantitative polymerase chain reaction cdna levels
    Relative mRNA abundance between B16F0 exosomes and cells were consistent between qRT-PCR and <t>microarray</t> analyses (a) The abundance of 10 genes (Kpnb1, Rnf14, Rnd2, Ptp4a3, Ptpn11, Eif2c2, Hipk2, Eif4ebp2, Dnmt3a, and Wsb2) in B16F0 exosomes versus B16F0 cells were quantified by quantitative RT-PCR (mean ± s.d., N = 3). The qRT-PCR results were normalized to the average differential abundance of three control genes: Kpnb1, Rnf14, and Rnd2. (b) The relative abundances of mRNAs assayed by qRT-PCR were compared against the relative abundances of mRNAs assayed by <t>cDNA</t> microarray. The dotted line indicates that the two different assays provide the same results for relative abundance. (c) Full-length coding sequences (ORFs) were amplified by semi-quantitative RT-PCR. Equal concentrations of RNA were reverse-transcribed into cDNA and amplified by PCR. After 25 cycles, full-length open-reading frame amplicons were monitored every three cycles and resolved on agarose gel before the amplification was saturated.
    Quantitative Polymerase Chain Reaction Cdna Levels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc gene expression level analysis rafflesia cdna illumina
    Relative mRNA abundance between B16F0 exosomes and cells were consistent between qRT-PCR and <t>microarray</t> analyses (a) The abundance of 10 genes (Kpnb1, Rnf14, Rnd2, Ptp4a3, Ptpn11, Eif2c2, Hipk2, Eif4ebp2, Dnmt3a, and Wsb2) in B16F0 exosomes versus B16F0 cells were quantified by quantitative RT-PCR (mean ± s.d., N = 3). The qRT-PCR results were normalized to the average differential abundance of three control genes: Kpnb1, Rnf14, and Rnd2. (b) The relative abundances of mRNAs assayed by qRT-PCR were compared against the relative abundances of mRNAs assayed by <t>cDNA</t> microarray. The dotted line indicates that the two different assays provide the same results for relative abundance. (c) Full-length coding sequences (ORFs) were amplified by semi-quantitative RT-PCR. Equal concentrations of RNA were reverse-transcribed into cDNA and amplified by PCR. After 25 cycles, full-length open-reading frame amplicons were monitored every three cycles and resolved on agarose gel before the amplification was saturated.
    Gene Expression Level Analysis Rafflesia Cdna Illumina, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Validation by quantitative on-line RT-PCR analyses of the differential expression between sweetpotato initiating storage and fibrous roots revealed by the use of Illumina-based sequencing at the cDNA level. Expression levels were measured in initiating storage root (ISR) and fibrous root (FR) samples of Georgia Jet sweetpotato variety, using at least four biological replicates. Each replicate consisted of cDNA representing pooled root tissue from 30 plants. Quantitative RT-PCR was performed and values were normalized relative to the expression levels of 18S rRNA in the same cDNA sample. Expression data are the means (± SE) of at least four replicates and are presented as relative expression values of the respective gene in the ISR sample relative to its expression in the FR sample (ISR/FR). The Y axis has a logarithmic scale. ADP glucose pyrophosphorylase – AGPase; coumaroyl-CoA synthase – 4CL; caffeoyl-CoA O-methyltransferase – CCoAOMT; cinnamyl alcohol dehydrogenase – CAD.

    Journal: BMC Genomics

    Article Title: Transcriptional profiling of sweetpotato (Ipomoea batatas) roots indicates down-regulation of lignin biosynthesis and up-regulation of starch biosynthesis at an early stage of storage root formation

    doi: 10.1186/1471-2164-14-460

    Figure Lengend Snippet: Validation by quantitative on-line RT-PCR analyses of the differential expression between sweetpotato initiating storage and fibrous roots revealed by the use of Illumina-based sequencing at the cDNA level. Expression levels were measured in initiating storage root (ISR) and fibrous root (FR) samples of Georgia Jet sweetpotato variety, using at least four biological replicates. Each replicate consisted of cDNA representing pooled root tissue from 30 plants. Quantitative RT-PCR was performed and values were normalized relative to the expression levels of 18S rRNA in the same cDNA sample. Expression data are the means (± SE) of at least four replicates and are presented as relative expression values of the respective gene in the ISR sample relative to its expression in the FR sample (ISR/FR). The Y axis has a logarithmic scale. ADP glucose pyrophosphorylase – AGPase; coumaroyl-CoA synthase – 4CL; caffeoyl-CoA O-methyltransferase – CCoAOMT; cinnamyl alcohol dehydrogenase – CAD.

    Article Snippet: Validation of differential expression between ISRs and FRs revealed by the use of Illumina-based sequencing at the cDNA level The validity of the expression differences detected by Illumina-based sequencing was examined at the RNA level, using on-line quantitative (q) RT-PCR.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Sequencing, Quantitative RT-PCR

    Relative mRNA abundance between B16F0 exosomes and cells were consistent between qRT-PCR and microarray analyses (a) The abundance of 10 genes (Kpnb1, Rnf14, Rnd2, Ptp4a3, Ptpn11, Eif2c2, Hipk2, Eif4ebp2, Dnmt3a, and Wsb2) in B16F0 exosomes versus B16F0 cells were quantified by quantitative RT-PCR (mean ± s.d., N = 3). The qRT-PCR results were normalized to the average differential abundance of three control genes: Kpnb1, Rnf14, and Rnd2. (b) The relative abundances of mRNAs assayed by qRT-PCR were compared against the relative abundances of mRNAs assayed by cDNA microarray. The dotted line indicates that the two different assays provide the same results for relative abundance. (c) Full-length coding sequences (ORFs) were amplified by semi-quantitative RT-PCR. Equal concentrations of RNA were reverse-transcribed into cDNA and amplified by PCR. After 25 cycles, full-length open-reading frame amplicons were monitored every three cycles and resolved on agarose gel before the amplification was saturated.

    Journal: Pigment cell & melanoma research

    Article Title: Melanoma exosomes deliver a complex biological payload that upregulates PTPN11 to suppress T lymphocyte function

    doi: 10.1111/pcmr.12564

    Figure Lengend Snippet: Relative mRNA abundance between B16F0 exosomes and cells were consistent between qRT-PCR and microarray analyses (a) The abundance of 10 genes (Kpnb1, Rnf14, Rnd2, Ptp4a3, Ptpn11, Eif2c2, Hipk2, Eif4ebp2, Dnmt3a, and Wsb2) in B16F0 exosomes versus B16F0 cells were quantified by quantitative RT-PCR (mean ± s.d., N = 3). The qRT-PCR results were normalized to the average differential abundance of three control genes: Kpnb1, Rnf14, and Rnd2. (b) The relative abundances of mRNAs assayed by qRT-PCR were compared against the relative abundances of mRNAs assayed by cDNA microarray. The dotted line indicates that the two different assays provide the same results for relative abundance. (c) Full-length coding sequences (ORFs) were amplified by semi-quantitative RT-PCR. Equal concentrations of RNA were reverse-transcribed into cDNA and amplified by PCR. After 25 cycles, full-length open-reading frame amplicons were monitored every three cycles and resolved on agarose gel before the amplification was saturated.

    Article Snippet: Specifically, we used an exon-level cDNA Affymetrix microarray to quantify mRNA transcripts in B16F0 exosomes and the parental B16F0 cells.

    Techniques: Quantitative RT-PCR, Microarray, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis