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  • 99
    Thermo Fisher cdna fragments
    Cdna Fragments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore human trail cdna fragment
    Human Trail Cdna Fragment, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Illumina Inc cdna fragments
    Divergent primers detect circular RNAs in <t>cDNA</t> but not genomic <t>DNA</t> (gDNA).
    Cdna Fragments, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 5631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene cdna fragment
    (A) Schematic representation of the <t>VAV-3</t> cDNAs isolated in this study. Clones obtained from the screening of <t>cDNA</t> libraries are shown in regular typeface. Those obtained from RACE amplifications are shown in italics. The 5′ UTRs are shown as closed boxes. The 3′ UTRs are shown as open boxes and not in scale. The 5′ ends encoding the Vav-3 CH domain are shown as shaded boxes. Stop codons are indicated by asterisks. Start codons are indicated by inverted triangles. Important restriction sites for cloning purposes are indicated. (B) Alignment of the amino acid sequences of the three members of the Vav family in Homo sapiens . Identical residues present in the three proteins are shown on boldface. The individual structural domains are boxed. PRR, proline-rich region.
    Cdna Fragment, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies cdna fragments
    Dissection, single-cell isolation, and genome-wide transcriptional profiling of early embryonic mouse heart. Hearts from developing embryos at indicated developmental stages were harvested and dissected into zones as shown. Single cells from each zone underwent lysis, <t>RNA</t> extraction, and <t>cDNA</t> library generation before sequencing and bioinformatics analysis. Workflow for generation and analysis of scRNA-seq data Whole-mount microscopy of murine e8.5, 9.5, and 10.5 heart showing dissection zone boundaries. IFT - inflow tract, A – atrium, V – ventricle, RA – right atrium, LA – left atrium, AVC – atrioventricular canal, RV – right ventricle, RS – right ventricular septum, LS – left ventricular septum, LV – left ventricle, OFT – outflow tract, PO – proximal outflow tract, DO – distal outflow tract. Scale bars = 500 μm. Validation of previously reported chamber-specific genes in dissected e10.5 heart zones by bulk-tissue qPCR. t-SNE plots showing major clusters among all scRNA-seq samples at each stage of development. Color and number labeling indicate zone of origin for each cell. Due to the different number of zones and cells present at each stage, t-SNE plots of different stages should not be directly compared. I – inflow tract. )
    Cdna Fragments, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GenScript complementary dna fragments
    <t>CREB</t> inhibits MYCT1 transcription. Notes: ( A ) Transcription factor CREB binding sites present in the MYCT1 promoter region. ( B ) MYCT1 promoter activity analysis. Hep2 cells were transfected with pGL3-P760 or pGL3-P795, followed by a luciferase reporter assay. Firefly luciferase activity was normalized to Renilla luciferase activity. ( C ) Effect of CREB on pGL3-P795 activity. pcDNA3.1 vector or pcDNA3.1-CREB was co-transfected with pGL3-P795 into Hep2 cells, followed by a luciferase reporter assay. ( D ) Effect of CREB on MYCT1 mRNA level. MYCT1 mRNA level was detected by qRT-PCR in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( E ) Effect of CREB on MYCT1 protein level. MYCT1 protein level was examined by Western blot in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( F ) Binding of CREB to MYCT1 in vivo. <t>DNA</t> fragments from Hep2 cells were amplified by PCR based on immunoprecipitation with anti-IgG or anti-CREB antibodies. ** P
    Complementary Dna Fragments, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher filter cartridges tubes for cdna purification
    <t>CREB</t> inhibits MYCT1 transcription. Notes: ( A ) Transcription factor CREB binding sites present in the MYCT1 promoter region. ( B ) MYCT1 promoter activity analysis. Hep2 cells were transfected with pGL3-P760 or pGL3-P795, followed by a luciferase reporter assay. Firefly luciferase activity was normalized to Renilla luciferase activity. ( C ) Effect of CREB on pGL3-P795 activity. pcDNA3.1 vector or pcDNA3.1-CREB was co-transfected with pGL3-P795 into Hep2 cells, followed by a luciferase reporter assay. ( D ) Effect of CREB on MYCT1 mRNA level. MYCT1 mRNA level was detected by qRT-PCR in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( E ) Effect of CREB on MYCT1 protein level. MYCT1 protein level was examined by Western blot in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( F ) Binding of CREB to MYCT1 in vivo. <t>DNA</t> fragments from Hep2 cells were amplified by PCR based on immunoprecipitation with anti-IgG or anti-CREB antibodies. ** P
    Filter Cartridges Tubes For Cdna Purification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fragmented cdna
    <t>CREB</t> inhibits MYCT1 transcription. Notes: ( A ) Transcription factor CREB binding sites present in the MYCT1 promoter region. ( B ) MYCT1 promoter activity analysis. Hep2 cells were transfected with pGL3-P760 or pGL3-P795, followed by a luciferase reporter assay. Firefly luciferase activity was normalized to Renilla luciferase activity. ( C ) Effect of CREB on pGL3-P795 activity. pcDNA3.1 vector or pcDNA3.1-CREB was co-transfected with pGL3-P795 into Hep2 cells, followed by a luciferase reporter assay. ( D ) Effect of CREB on MYCT1 mRNA level. MYCT1 mRNA level was detected by qRT-PCR in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( E ) Effect of CREB on MYCT1 protein level. MYCT1 protein level was examined by Western blot in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( F ) Binding of CREB to MYCT1 in vivo. <t>DNA</t> fragments from Hep2 cells were amplified by PCR based on immunoprecipitation with anti-IgG or anti-CREB antibodies. ** P
    Fragmented Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Addgene inc cdna fragment
    <t>CREB</t> inhibits MYCT1 transcription. Notes: ( A ) Transcription factor CREB binding sites present in the MYCT1 promoter region. ( B ) MYCT1 promoter activity analysis. Hep2 cells were transfected with pGL3-P760 or pGL3-P795, followed by a luciferase reporter assay. Firefly luciferase activity was normalized to Renilla luciferase activity. ( C ) Effect of CREB on pGL3-P795 activity. pcDNA3.1 vector or pcDNA3.1-CREB was co-transfected with pGL3-P795 into Hep2 cells, followed by a luciferase reporter assay. ( D ) Effect of CREB on MYCT1 mRNA level. MYCT1 mRNA level was detected by qRT-PCR in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( E ) Effect of CREB on MYCT1 protein level. MYCT1 protein level was examined by Western blot in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( F ) Binding of CREB to MYCT1 in vivo. <t>DNA</t> fragments from Hep2 cells were amplified by PCR based on immunoprecipitation with anti-IgG or anti-CREB antibodies. ** P
    Cdna Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    OriGene cdna fragment
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Cdna Fragment, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim cdna fragments
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Cdna Fragments, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher solid dh10b fragment control library dna
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Solid Dh10b Fragment Control Library Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GenScript cdna fragment
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Cdna Fragment, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Solexa cdna fragments
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Cdna Fragments, supplied by Solexa, used in various techniques. Bioz Stars score: 93/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Unigene cdna fragments
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Cdna Fragments, supplied by Unigene, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Genewiz cdna fragment
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Cdna Fragment, supplied by Genewiz, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies cy5 labelled cdna fragments
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Cy5 Labelled Cdna Fragments, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore human tff3 cdna fragments
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Human Tff3 Cdna Fragments, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher plasmids encoding cdna fragments
    Construction of recombinant chimeric virus and wild-type <t>JEV</t> <t>cDNA</t> clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those
    Plasmids Encoding Cdna Fragments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Divergent primers detect circular RNAs in cDNA but not genomic DNA (gDNA).

    Journal: Frontiers in Oncology

    Article Title: Circular RNA Expression in Oral Squamous Cell Carcinoma

    doi: 10.3389/fonc.2018.00398

    Figure Lengend Snippet: Divergent primers detect circular RNAs in cDNA but not genomic DNA (gDNA).

    Article Snippet: Next, the cDNA fragments were purified with VAHTSTM DNA Clean Beads, end repaired, poly (A) added, and ligated to Illumina sequencing adapters.

    Techniques:

    (A) Schematic representation of the VAV-3 cDNAs isolated in this study. Clones obtained from the screening of cDNA libraries are shown in regular typeface. Those obtained from RACE amplifications are shown in italics. The 5′ UTRs are shown as closed boxes. The 3′ UTRs are shown as open boxes and not in scale. The 5′ ends encoding the Vav-3 CH domain are shown as shaded boxes. Stop codons are indicated by asterisks. Start codons are indicated by inverted triangles. Important restriction sites for cloning purposes are indicated. (B) Alignment of the amino acid sequences of the three members of the Vav family in Homo sapiens . Identical residues present in the three proteins are shown on boldface. The individual structural domains are boxed. PRR, proline-rich region.

    Journal: Molecular and Cellular Biology

    Article Title: Biological and Regulatory Properties of Vav-3, a New Member of the Vav Family of Oncoproteins

    doi:

    Figure Lengend Snippet: (A) Schematic representation of the VAV-3 cDNAs isolated in this study. Clones obtained from the screening of cDNA libraries are shown in regular typeface. Those obtained from RACE amplifications are shown in italics. The 5′ UTRs are shown as closed boxes. The 3′ UTRs are shown as open boxes and not in scale. The 5′ ends encoding the Vav-3 CH domain are shown as shaded boxes. Stop codons are indicated by asterisks. Start codons are indicated by inverted triangles. Important restriction sites for cloning purposes are indicated. (B) Alignment of the amino acid sequences of the three members of the Vav family in Homo sapiens . Identical residues present in the three proteins are shown on boldface. The individual structural domains are boxed. PRR, proline-rich region.

    Article Snippet: After obtaining the EST cDNA containing the VAV-3 3′ from the American Tissue Culture Collection, the cDNA fragment was used to screen a human placenta cDNA library (Stratagene).

    Techniques: Isolation, Clone Assay

    Dissection, single-cell isolation, and genome-wide transcriptional profiling of early embryonic mouse heart. Hearts from developing embryos at indicated developmental stages were harvested and dissected into zones as shown. Single cells from each zone underwent lysis, RNA extraction, and cDNA library generation before sequencing and bioinformatics analysis. Workflow for generation and analysis of scRNA-seq data Whole-mount microscopy of murine e8.5, 9.5, and 10.5 heart showing dissection zone boundaries. IFT - inflow tract, A – atrium, V – ventricle, RA – right atrium, LA – left atrium, AVC – atrioventricular canal, RV – right ventricle, RS – right ventricular septum, LS – left ventricular septum, LV – left ventricle, OFT – outflow tract, PO – proximal outflow tract, DO – distal outflow tract. Scale bars = 500 μm. Validation of previously reported chamber-specific genes in dissected e10.5 heart zones by bulk-tissue qPCR. t-SNE plots showing major clusters among all scRNA-seq samples at each stage of development. Color and number labeling indicate zone of origin for each cell. Due to the different number of zones and cells present at each stage, t-SNE plots of different stages should not be directly compared. I – inflow tract. )

    Journal: Developmental cell

    Article Title: Transcriptomic profiling maps anatomically patterned subpopulations among single embryonic cardiac cells

    doi: 10.1016/j.devcel.2016.10.014

    Figure Lengend Snippet: Dissection, single-cell isolation, and genome-wide transcriptional profiling of early embryonic mouse heart. Hearts from developing embryos at indicated developmental stages were harvested and dissected into zones as shown. Single cells from each zone underwent lysis, RNA extraction, and cDNA library generation before sequencing and bioinformatics analysis. Workflow for generation and analysis of scRNA-seq data Whole-mount microscopy of murine e8.5, 9.5, and 10.5 heart showing dissection zone boundaries. IFT - inflow tract, A – atrium, V – ventricle, RA – right atrium, LA – left atrium, AVC – atrioventricular canal, RV – right ventricle, RS – right ventricular septum, LS – left ventricular septum, LV – left ventricle, OFT – outflow tract, PO – proximal outflow tract, DO – distal outflow tract. Scale bars = 500 μm. Validation of previously reported chamber-specific genes in dissected e10.5 heart zones by bulk-tissue qPCR. t-SNE plots showing major clusters among all scRNA-seq samples at each stage of development. Color and number labeling indicate zone of origin for each cell. Due to the different number of zones and cells present at each stage, t-SNE plots of different stages should not be directly compared. I – inflow tract. )

    Article Snippet: To synthesize RNA probes, cDNA fragments (400bp-600bp) of candidate genes were cloned into pBluescript-SK vector (Agilent Technologies) or pGEM-Teasy vector (Promega).

    Techniques: Dissection, Single-cell Isolation, Genome Wide, Lysis, RNA Extraction, cDNA Library Assay, Sequencing, Microscopy, Real-time Polymerase Chain Reaction, Labeling

    CREB inhibits MYCT1 transcription. Notes: ( A ) Transcription factor CREB binding sites present in the MYCT1 promoter region. ( B ) MYCT1 promoter activity analysis. Hep2 cells were transfected with pGL3-P760 or pGL3-P795, followed by a luciferase reporter assay. Firefly luciferase activity was normalized to Renilla luciferase activity. ( C ) Effect of CREB on pGL3-P795 activity. pcDNA3.1 vector or pcDNA3.1-CREB was co-transfected with pGL3-P795 into Hep2 cells, followed by a luciferase reporter assay. ( D ) Effect of CREB on MYCT1 mRNA level. MYCT1 mRNA level was detected by qRT-PCR in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( E ) Effect of CREB on MYCT1 protein level. MYCT1 protein level was examined by Western blot in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( F ) Binding of CREB to MYCT1 in vivo. DNA fragments from Hep2 cells were amplified by PCR based on immunoprecipitation with anti-IgG or anti-CREB antibodies. ** P

    Journal: OncoTargets and therapy

    Article Title: CREB promotes laryngeal cancer cell migration via MYCT1/NAT10 axis

    doi: 10.2147/OTT.S156582

    Figure Lengend Snippet: CREB inhibits MYCT1 transcription. Notes: ( A ) Transcription factor CREB binding sites present in the MYCT1 promoter region. ( B ) MYCT1 promoter activity analysis. Hep2 cells were transfected with pGL3-P760 or pGL3-P795, followed by a luciferase reporter assay. Firefly luciferase activity was normalized to Renilla luciferase activity. ( C ) Effect of CREB on pGL3-P795 activity. pcDNA3.1 vector or pcDNA3.1-CREB was co-transfected with pGL3-P795 into Hep2 cells, followed by a luciferase reporter assay. ( D ) Effect of CREB on MYCT1 mRNA level. MYCT1 mRNA level was detected by qRT-PCR in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( E ) Effect of CREB on MYCT1 protein level. MYCT1 protein level was examined by Western blot in Hep2 cells transfected with pcDNA3.1 or pcDNA3.1-CREB. ( F ) Binding of CREB to MYCT1 in vivo. DNA fragments from Hep2 cells were amplified by PCR based on immunoprecipitation with anti-IgG or anti-CREB antibodies. ** P

    Article Snippet: Complementary DNA fragments encoding human CREB, MYCT1, and NAT10 were constructed into pcDNA3.1 vector (GenScript Company).

    Techniques: Binding Assay, Activity Assay, Transfection, Luciferase, Reporter Assay, Plasmid Preparation, Quantitative RT-PCR, Western Blot, In Vivo, Amplification, Polymerase Chain Reaction, Immunoprecipitation

    Construction of recombinant chimeric virus and wild-type JEV cDNA clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those

    Journal: Vaccine

    Article Title: Japanese encephalitis virus vaccine candidates generated by chimerization with dengue virus type 4

    doi: 10.1016/j.vaccine.2014.03.062

    Figure Lengend Snippet: Construction of recombinant chimeric virus and wild-type JEV cDNA clones. (A) For chimeric viruses, the C, prM and E genes, or just the prM and E genes of rDEN4 and rDEN4Δ30 cDNA clones (p4 and p4Δ30, respectively) were replaced with those

    Article Snippet: A cDNA fragment corresponding to the JEV India/78C, prM, and E genes was synthesized and cloned into mammalian expression vector pCMV6-AC (OriGene) to generate pJEV-CprME.

    Techniques: Recombinant, Clone Assay