Journal: Frontiers in Plant Science
Article Title: A Combined Comparative Transcriptomic, Metabolomic, and Anatomical Analyses of Two Key Domestication Traits: Pod Dehiscence and Seed Dormancy in Pea (Pisum sp.)
Figure Lengend Snippet: qRT-PCR validation of selected DEGs detected to be associated with pod dehiscence. (A) MACE results in comparison with qRT-PCR of selected candidate genes. MACE-P004, MACE-P013, and MACE-P015 shows the same trend of over/down expression between MACE results of parental lines and RILs (JI 64—dehiscent pod, bulk of dehiscent RILs, JI 92—indehiscent pod, bulk of indehiscent RILs) and qRT-PCR of cDNA of parental lines (ventral or dorsal pod suture in two stages: I—younger stage, II—older stage). MACE-P009 showed the opposite results and MACE-P017 and MACE-P0018 without any trend of down or over expression in qRT-PCR. (B) qRT-PCR results of contrasting RILs (dehiscent/indehiscent pod) in comparison with parental lines of candidate gene MACE-P015. (C) qRT-PCR results of contrasting RILs (dehiscent/indehiscent pod) in comparison with parental lines of candidate genes SHATTERPROOF and SHATTERING.
Article Snippet: Massive analysis of cDNA ends (MACE) MACE libraries were generated using GenXPro's MACE kit (GenXPro GmbH, Frankfurt, Germany) as described in Zawada et al. ( ).
Techniques: Quantitative RT-PCR, Expressing, Over Expression