cdna Thermo Fisher Search Results


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  • 99
    Thermo Fisher complementary dna cdna
    Complementary Dna Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher complementary dna
    Complementary Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17822 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher complementary dna cdna synthesis
    Complementary Dna Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3779 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher complementary dna cdna template
    Expression levels of <t>XYLP</t> mRNA in different human tissues. Multiple tissue <t>cDNA</t> panels (Clontech) were used to perform real time PCR analyses. Levels of XYLP ( open bars ) and FAM20B ( closed bars ) mRNAs were normalized to those of glyceraldehyde-3-phosphate
    Complementary Dna Cdna Template, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher transcriptomic complementary dna cdna
    Expression levels of <t>XYLP</t> mRNA in different human tissues. Multiple tissue <t>cDNA</t> panels (Clontech) were used to perform real time PCR analyses. Levels of XYLP ( open bars ) and FAM20B ( closed bars ) mRNAs were normalized to those of glyceraldehyde-3-phosphate
    Transcriptomic Complementary Dna Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher complementary dna cdna conversion kit
    Expression levels of <t>XYLP</t> mRNA in different human tissues. Multiple tissue <t>cDNA</t> panels (Clontech) were used to perform real time PCR analyses. Levels of XYLP ( open bars ) and FAM20B ( closed bars ) mRNAs were normalized to those of glyceraldehyde-3-phosphate
    Complementary Dna Cdna Conversion Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher complementary dna cdna synthesis kit
    Expression levels of <t>XYLP</t> mRNA in different human tissues. Multiple tissue <t>cDNA</t> panels (Clontech) were used to perform real time PCR analyses. Levels of XYLP ( open bars ) and FAM20B ( closed bars ) mRNAs were normalized to those of glyceraldehyde-3-phosphate
    Complementary Dna Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher complementary dna cdna synthesis supermix kit
    Expression levels of <t>XYLP</t> mRNA in different human tissues. Multiple tissue <t>cDNA</t> panels (Clontech) were used to perform real time PCR analyses. Levels of XYLP ( open bars ) and FAM20B ( closed bars ) mRNAs were normalized to those of glyceraldehyde-3-phosphate
    Complementary Dna Cdna Synthesis Supermix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher complementary dna cdna master mix
    Expression levels of <t>XYLP</t> mRNA in different human tissues. Multiple tissue <t>cDNA</t> panels (Clontech) were used to perform real time PCR analyses. Levels of XYLP ( open bars ) and FAM20B ( closed bars ) mRNAs were normalized to those of glyceraldehyde-3-phosphate
    Complementary Dna Cdna Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher complementary dna cdna lysis buffer
    Regulation of global histone 3 lysine 27 trimethylation (H3K27me3) levels in osteoarthritis (OA) and adult articular cartilage. a Jumonji domain-containing 3 ( JMJD3 ) was increased in damaged cartilage compared with undamaged cartilage from within the same knees of patients undergoing unicompartmental knee replacement for OA ( n = 5 patients). b Inhibition of H3K27me3 demethylases by treatment of human articular chondrocyte (HACs) with GSK-J4 for 24 h caused a decrease in matrix metallopeptidase 13 ( MMP13 ), prostaglandin-endoperoxide synthase 2 ( PTGS2 ) and collagen type X, alpha 1 ( COL10A1 ) expression ( n = 4 patients, 4 technical replicates per condition). Dashed line shows control expression of each gene. c HACs were treated for 72 h with small interfering RNA (siRNA) against JMJD3, lysine (K)-specific demethylase 6A (UTX) and non-targeting siRNA control prior to RNA extraction and complementary <t>DNA</t> synthesis ( n = 4 patients, n = 4 technical replicates per patient). Expression of MMP13 , PTGS2 and COL10A1 was assessed by quantitative reverse transcription polymerase chain reaction. Dashed line shows expression level following treatment with non-targeting siRNA control. d Interleukin (IL)-1/oncostatin M (OSM)-induced proteoglycan loss from human articular cartilage explants was reduced in the presence of GSK-J4 ( n = 5 patients, 3 technical replicates per condition). e Treatment of HACs with IL-1, IL-6 and transforming growth factor (TGF)-β increased JMJD3 expression. f Expression of PAI1 , JMJD3 and MMP13 following 6-h treatment of HACs with TGF-β with or without GSK-J4 ( n = 4 patients, 4 technical replicates per condition). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Data are presented as individual data points for biological replicates showing mean ± 95 % CI. a Paired t test, b – f Analysis of variance with Dunnett’s correction for multiple comparisons. GAG glycosaminoglycan
    Complementary Dna Cdna Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher complementary dna oligonucleotides
    Regulation of global histone 3 lysine 27 trimethylation (H3K27me3) levels in osteoarthritis (OA) and adult articular cartilage. a Jumonji domain-containing 3 ( JMJD3 ) was increased in damaged cartilage compared with undamaged cartilage from within the same knees of patients undergoing unicompartmental knee replacement for OA ( n = 5 patients). b Inhibition of H3K27me3 demethylases by treatment of human articular chondrocyte (HACs) with GSK-J4 for 24 h caused a decrease in matrix metallopeptidase 13 ( MMP13 ), prostaglandin-endoperoxide synthase 2 ( PTGS2 ) and collagen type X, alpha 1 ( COL10A1 ) expression ( n = 4 patients, 4 technical replicates per condition). Dashed line shows control expression of each gene. c HACs were treated for 72 h with small interfering RNA (siRNA) against JMJD3, lysine (K)-specific demethylase 6A (UTX) and non-targeting siRNA control prior to RNA extraction and complementary <t>DNA</t> synthesis ( n = 4 patients, n = 4 technical replicates per patient). Expression of MMP13 , PTGS2 and COL10A1 was assessed by quantitative reverse transcription polymerase chain reaction. Dashed line shows expression level following treatment with non-targeting siRNA control. d Interleukin (IL)-1/oncostatin M (OSM)-induced proteoglycan loss from human articular cartilage explants was reduced in the presence of GSK-J4 ( n = 5 patients, 3 technical replicates per condition). e Treatment of HACs with IL-1, IL-6 and transforming growth factor (TGF)-β increased JMJD3 expression. f Expression of PAI1 , JMJD3 and MMP13 following 6-h treatment of HACs with TGF-β with or without GSK-J4 ( n = 4 patients, 4 technical replicates per condition). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Data are presented as individual data points for biological replicates showing mean ± 95 % CI. a Paired t test, b – f Analysis of variance with Dunnett’s correction for multiple comparisons. GAG glycosaminoglycan
    Complementary Dna Oligonucleotides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher complementary dna synthesis system
    Regulation of global histone 3 lysine 27 trimethylation (H3K27me3) levels in osteoarthritis (OA) and adult articular cartilage. a Jumonji domain-containing 3 ( JMJD3 ) was increased in damaged cartilage compared with undamaged cartilage from within the same knees of patients undergoing unicompartmental knee replacement for OA ( n = 5 patients). b Inhibition of H3K27me3 demethylases by treatment of human articular chondrocyte (HACs) with GSK-J4 for 24 h caused a decrease in matrix metallopeptidase 13 ( MMP13 ), prostaglandin-endoperoxide synthase 2 ( PTGS2 ) and collagen type X, alpha 1 ( COL10A1 ) expression ( n = 4 patients, 4 technical replicates per condition). Dashed line shows control expression of each gene. c HACs were treated for 72 h with small interfering RNA (siRNA) against JMJD3, lysine (K)-specific demethylase 6A (UTX) and non-targeting siRNA control prior to RNA extraction and complementary <t>DNA</t> synthesis ( n = 4 patients, n = 4 technical replicates per patient). Expression of MMP13 , PTGS2 and COL10A1 was assessed by quantitative reverse transcription polymerase chain reaction. Dashed line shows expression level following treatment with non-targeting siRNA control. d Interleukin (IL)-1/oncostatin M (OSM)-induced proteoglycan loss from human articular cartilage explants was reduced in the presence of GSK-J4 ( n = 5 patients, 3 technical replicates per condition). e Treatment of HACs with IL-1, IL-6 and transforming growth factor (TGF)-β increased JMJD3 expression. f Expression of PAI1 , JMJD3 and MMP13 following 6-h treatment of HACs with TGF-β with or without GSK-J4 ( n = 4 patients, 4 technical replicates per condition). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Data are presented as individual data points for biological replicates showing mean ± 95 % CI. a Paired t test, b – f Analysis of variance with Dunnett’s correction for multiple comparisons. GAG glycosaminoglycan
    Complementary Dna Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher first strand complementary dna
    Regulation of global histone 3 lysine 27 trimethylation (H3K27me3) levels in osteoarthritis (OA) and adult articular cartilage. a Jumonji domain-containing 3 ( JMJD3 ) was increased in damaged cartilage compared with undamaged cartilage from within the same knees of patients undergoing unicompartmental knee replacement for OA ( n = 5 patients). b Inhibition of H3K27me3 demethylases by treatment of human articular chondrocyte (HACs) with GSK-J4 for 24 h caused a decrease in matrix metallopeptidase 13 ( MMP13 ), prostaglandin-endoperoxide synthase 2 ( PTGS2 ) and collagen type X, alpha 1 ( COL10A1 ) expression ( n = 4 patients, 4 technical replicates per condition). Dashed line shows control expression of each gene. c HACs were treated for 72 h with small interfering RNA (siRNA) against JMJD3, lysine (K)-specific demethylase 6A (UTX) and non-targeting siRNA control prior to RNA extraction and complementary <t>DNA</t> synthesis ( n = 4 patients, n = 4 technical replicates per patient). Expression of MMP13 , PTGS2 and COL10A1 was assessed by quantitative reverse transcription polymerase chain reaction. Dashed line shows expression level following treatment with non-targeting siRNA control. d Interleukin (IL)-1/oncostatin M (OSM)-induced proteoglycan loss from human articular cartilage explants was reduced in the presence of GSK-J4 ( n = 5 patients, 3 technical replicates per condition). e Treatment of HACs with IL-1, IL-6 and transforming growth factor (TGF)-β increased JMJD3 expression. f Expression of PAI1 , JMJD3 and MMP13 following 6-h treatment of HACs with TGF-β with or without GSK-J4 ( n = 4 patients, 4 technical replicates per condition). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Data are presented as individual data points for biological replicates showing mean ± 95 % CI. a Paired t test, b – f Analysis of variance with Dunnett’s correction for multiple comparisons. GAG glycosaminoglycan
    First Strand Complementary Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher human mcm7 complementary dna cdna
    Pb/MCM7i13 transgenic construct. (A) Pb, prostate-specific rat probasin promoter ARR 2 PB; black box, HA tag; white box, Xpress tag; gray boxes, human <t>MCM7</t> coding sequence; red and blue boxes, miR-93/106b and miR-25 genes; yellow box, SV40 poly(A) (SV40 pA); orange box, probe used for Southern analysis of transgenic lines; green arrows, primers used for PCR analysis of transgenic lines and for genotyping. (B) Top: Southern blot analysis. Genomic <t>DNA</t> extracted from tails was digested with Bam HI and hybridized with a probe complementary to the SV40 poly(A) region as shown in (A). The Bam HI–digested Pb/MCM7i13 plasmid was used as positive control (C+). Bottom: PCR analysis. A forward primer complementary to the probasin promoter and a reverse primer complementary to the tag were used, as shown in (A). Empty Pb and Pb/MCM7i13 plasmids were used as negative (C−) and positive (C+) controls. L, 100-bp ladder. Three representative positive (P) and negative (N) mice are shown. (C) Abundance of the transgenic construct measured by real-time PCR in nine different lines. A primer pair specific for human MCM7 coding sequence was used. LE, ME, HE: low, medium, and high expressors, respectively. Human MCM7 .
    Human Mcm7 Complementary Dna Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher brain complementary dna
    Pb/MCM7i13 transgenic construct. (A) Pb, prostate-specific rat probasin promoter ARR 2 PB; black box, HA tag; white box, Xpress tag; gray boxes, human <t>MCM7</t> coding sequence; red and blue boxes, miR-93/106b and miR-25 genes; yellow box, SV40 poly(A) (SV40 pA); orange box, probe used for Southern analysis of transgenic lines; green arrows, primers used for PCR analysis of transgenic lines and for genotyping. (B) Top: Southern blot analysis. Genomic <t>DNA</t> extracted from tails was digested with Bam HI and hybridized with a probe complementary to the SV40 poly(A) region as shown in (A). The Bam HI–digested Pb/MCM7i13 plasmid was used as positive control (C+). Bottom: PCR analysis. A forward primer complementary to the probasin promoter and a reverse primer complementary to the tag were used, as shown in (A). Empty Pb and Pb/MCM7i13 plasmids were used as negative (C−) and positive (C+) controls. L, 100-bp ladder. Three representative positive (P) and negative (N) mice are shown. (C) Abundance of the transgenic construct measured by real-time PCR in nine different lines. A primer pair specific for human MCM7 coding sequence was used. LE, ME, HE: low, medium, and high expressors, respectively. Human MCM7 .
    Brain Complementary Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher capacity complementary dna cdna archive kit
    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
    Capacity Complementary Dna Cdna Archive Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher human crbn complementary dna
    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
    Human Crbn Complementary Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high capacity complementary dna kit
    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
    High Capacity Complementary Dna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi high capacity complementary dna cdna kit
    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
    Abi High Capacity Complementary Dna Cdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher capacity complementary dna transcription kit
    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
    Capacity Complementary Dna Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher first strand complementary dna synthesis
    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
    First Strand Complementary Dna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher complementary dna synthesis trizol regent
    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
    Complementary Dna Synthesis Trizol Regent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
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    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
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    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
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    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix <t>DNA</t> microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total <t>RNA</t> for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were
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    Image Search Results


    Expression levels of XYLP mRNA in different human tissues. Multiple tissue cDNA panels (Clontech) were used to perform real time PCR analyses. Levels of XYLP ( open bars ) and FAM20B ( closed bars ) mRNAs were normalized to those of glyceraldehyde-3-phosphate

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of Phosphatase That Dephosphorylates Xylose in the Glycosaminoglycan-Protein Linkage Region of Proteoglycans *

    doi: 10.1074/jbc.M113.520536

    Figure Lengend Snippet: Expression levels of XYLP mRNA in different human tissues. Multiple tissue cDNA panels (Clontech) were used to perform real time PCR analyses. Levels of XYLP ( open bars ) and FAM20B ( closed bars ) mRNAs were normalized to those of glyceraldehyde-3-phosphate

    Article Snippet: PCR was used to amplify a cDNA fragment predicted to encode a truncated form of the putative phosphatase (XYLP) lacking the first 37 N-terminal amino acids; the template cDNA was obtained from Open Biosystems (MHS1010-7508558, corresponding to the human acid phosphatase-like-2 (ACPL2) cDNA in GenBankTM ); the 5′-primer (5′-GAAGATCTGGAATGAGTAGCAAGAGTCGA-3′) contained an in-frame BglII site, and the 3′-primer (5′-GAAGATCTGTGGACCTTTCCCTATGCTCT-3′) contained a BglII site located 38 bp downstream of the predicted stop codon.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Regulation of global histone 3 lysine 27 trimethylation (H3K27me3) levels in osteoarthritis (OA) and adult articular cartilage. a Jumonji domain-containing 3 ( JMJD3 ) was increased in damaged cartilage compared with undamaged cartilage from within the same knees of patients undergoing unicompartmental knee replacement for OA ( n = 5 patients). b Inhibition of H3K27me3 demethylases by treatment of human articular chondrocyte (HACs) with GSK-J4 for 24 h caused a decrease in matrix metallopeptidase 13 ( MMP13 ), prostaglandin-endoperoxide synthase 2 ( PTGS2 ) and collagen type X, alpha 1 ( COL10A1 ) expression ( n = 4 patients, 4 technical replicates per condition). Dashed line shows control expression of each gene. c HACs were treated for 72 h with small interfering RNA (siRNA) against JMJD3, lysine (K)-specific demethylase 6A (UTX) and non-targeting siRNA control prior to RNA extraction and complementary DNA synthesis ( n = 4 patients, n = 4 technical replicates per patient). Expression of MMP13 , PTGS2 and COL10A1 was assessed by quantitative reverse transcription polymerase chain reaction. Dashed line shows expression level following treatment with non-targeting siRNA control. d Interleukin (IL)-1/oncostatin M (OSM)-induced proteoglycan loss from human articular cartilage explants was reduced in the presence of GSK-J4 ( n = 5 patients, 3 technical replicates per condition). e Treatment of HACs with IL-1, IL-6 and transforming growth factor (TGF)-β increased JMJD3 expression. f Expression of PAI1 , JMJD3 and MMP13 following 6-h treatment of HACs with TGF-β with or without GSK-J4 ( n = 4 patients, 4 technical replicates per condition). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Data are presented as individual data points for biological replicates showing mean ± 95 % CI. a Paired t test, b – f Analysis of variance with Dunnett’s correction for multiple comparisons. GAG glycosaminoglycan

    Journal: Arthritis Research & Therapy

    Article Title: H3K27me3 demethylases regulate in vitro chondrogenesis and chondrocyte activity in osteoarthritis

    doi: 10.1186/s13075-016-1053-7

    Figure Lengend Snippet: Regulation of global histone 3 lysine 27 trimethylation (H3K27me3) levels in osteoarthritis (OA) and adult articular cartilage. a Jumonji domain-containing 3 ( JMJD3 ) was increased in damaged cartilage compared with undamaged cartilage from within the same knees of patients undergoing unicompartmental knee replacement for OA ( n = 5 patients). b Inhibition of H3K27me3 demethylases by treatment of human articular chondrocyte (HACs) with GSK-J4 for 24 h caused a decrease in matrix metallopeptidase 13 ( MMP13 ), prostaglandin-endoperoxide synthase 2 ( PTGS2 ) and collagen type X, alpha 1 ( COL10A1 ) expression ( n = 4 patients, 4 technical replicates per condition). Dashed line shows control expression of each gene. c HACs were treated for 72 h with small interfering RNA (siRNA) against JMJD3, lysine (K)-specific demethylase 6A (UTX) and non-targeting siRNA control prior to RNA extraction and complementary DNA synthesis ( n = 4 patients, n = 4 technical replicates per patient). Expression of MMP13 , PTGS2 and COL10A1 was assessed by quantitative reverse transcription polymerase chain reaction. Dashed line shows expression level following treatment with non-targeting siRNA control. d Interleukin (IL)-1/oncostatin M (OSM)-induced proteoglycan loss from human articular cartilage explants was reduced in the presence of GSK-J4 ( n = 5 patients, 3 technical replicates per condition). e Treatment of HACs with IL-1, IL-6 and transforming growth factor (TGF)-β increased JMJD3 expression. f Expression of PAI1 , JMJD3 and MMP13 following 6-h treatment of HACs with TGF-β with or without GSK-J4 ( n = 4 patients, 4 technical replicates per condition). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Data are presented as individual data points for biological replicates showing mean ± 95 % CI. a Paired t test, b – f Analysis of variance with Dunnett’s correction for multiple comparisons. GAG glycosaminoglycan

    Article Snippet: Treated HACs and MSCs were washed twice in PBS, and cells were harvested in cells to complementary DNA (cDNA) lysis buffer (Ambion; Thermo Fisher Scientific, Austin, TX, USA) prior to cDNA synthesis.

    Techniques: Inhibition, Expressing, Small Interfering RNA, RNA Extraction, DNA Synthesis, Reverse Transcription Polymerase Chain Reaction

    Pb/MCM7i13 transgenic construct. (A) Pb, prostate-specific rat probasin promoter ARR 2 PB; black box, HA tag; white box, Xpress tag; gray boxes, human MCM7 coding sequence; red and blue boxes, miR-93/106b and miR-25 genes; yellow box, SV40 poly(A) (SV40 pA); orange box, probe used for Southern analysis of transgenic lines; green arrows, primers used for PCR analysis of transgenic lines and for genotyping. (B) Top: Southern blot analysis. Genomic DNA extracted from tails was digested with Bam HI and hybridized with a probe complementary to the SV40 poly(A) region as shown in (A). The Bam HI–digested Pb/MCM7i13 plasmid was used as positive control (C+). Bottom: PCR analysis. A forward primer complementary to the probasin promoter and a reverse primer complementary to the tag were used, as shown in (A). Empty Pb and Pb/MCM7i13 plasmids were used as negative (C−) and positive (C+) controls. L, 100-bp ladder. Three representative positive (P) and negative (N) mice are shown. (C) Abundance of the transgenic construct measured by real-time PCR in nine different lines. A primer pair specific for human MCM7 coding sequence was used. LE, ME, HE: low, medium, and high expressors, respectively. Human MCM7 .

    Journal: Science signaling

    Article Title: Identification of the miR-106b~25 MicroRNA Cluster as a Proto-Oncogenic PTEN-Targeting Intron That Cooperates with Its Host Gene MCM7 in Transformation

    doi: 10.1126/scisignal.2000594

    Figure Lengend Snippet: Pb/MCM7i13 transgenic construct. (A) Pb, prostate-specific rat probasin promoter ARR 2 PB; black box, HA tag; white box, Xpress tag; gray boxes, human MCM7 coding sequence; red and blue boxes, miR-93/106b and miR-25 genes; yellow box, SV40 poly(A) (SV40 pA); orange box, probe used for Southern analysis of transgenic lines; green arrows, primers used for PCR analysis of transgenic lines and for genotyping. (B) Top: Southern blot analysis. Genomic DNA extracted from tails was digested with Bam HI and hybridized with a probe complementary to the SV40 poly(A) region as shown in (A). The Bam HI–digested Pb/MCM7i13 plasmid was used as positive control (C+). Bottom: PCR analysis. A forward primer complementary to the probasin promoter and a reverse primer complementary to the tag were used, as shown in (A). Empty Pb and Pb/MCM7i13 plasmids were used as negative (C−) and positive (C+) controls. L, 100-bp ladder. Three representative positive (P) and negative (N) mice are shown. (C) Abundance of the transgenic construct measured by real-time PCR in nine different lines. A primer pair specific for human MCM7 coding sequence was used. LE, ME, HE: low, medium, and high expressors, respectively. Human MCM7 .

    Article Snippet: The following antibodies and reagents were used: antibodies against Hsp-90 #61041 (Becton Dickinson); PTEN #9559 (WB), pAkt (Ser473 ) #9271 (WB), #3787 (IHC), Akt #9272, pS6 #2211 (Cell Signaling); DICER #CG006 (Clonegene); MSCV-neo (Clontech); siGENOME non-targeting small interfering RNA (siRNA) #2 (si-Luc), si-miR-19a, si-miR-19b, si-miR-22, si-miR-25, si-miR-92a, si-miR-17, si-miR-20a, si-miR-93, si-miR-106b, si-miR-302a, si-miR-372, si-miR-373, si-PTEN, siGLO RISC-free control siRNA miRNA antisense inhibitor negative control #1, miR-19a antisense inhibitor, miR-22 antisense inhibitor, miR-25 antisense inhibitor, miR-93 antisense inhibitor, miR-106b antisense inhibitor, Dharmafect 1 (Dharmacon); miR-17, miR-19a, miR-20a, miR-22, miR-25, miR-93, and miR-106b 3′ digoxigenin (DIG)–labeled LNA ISH probes (Exiqon); lipofectamine 2000, Trizol reagent, deoxyribonuclease I (DNase I) amplification grade, SuperScript II reverse transcriptase, Dulbecco's modified Eagle's medium (DMEM), RPMI 1640, fetal bovine serum (FBS), keratinocyte serum-free medium, epidermal growth factor (EGF), bovine pituitary extract (BPE), antibody against Xpress Tag, pcDNA3.1/His C, geneticin (G418), hygromycin, antibody against PTEN (IHC) #51-2400 (Invitrogen); human MCM7 complementary DNA (cDNA) #MHS1011-75176 (Open Biosystems); pGL3-Control ( Firefly luciferase ), pRL-TK ( Renilla luciferase ), Dual-Luciferase reporter assay (Promega); fraction V bovine serum albumin (BSA), polybrene, insulin, antibody against actin #A3853, anti-FLAG antibody #F3165, puromycin (Sigma); QuantiTect Sybr Green PCRkit (Qiagen); antibody against MCM7 #9966 (Santa Cruz Biotechnology); QuikChange II XL Site-Directed Mutagenesis Kit, Herculase Taq polymerase (Stratagene); Ki67 antibody #VP-K451 (Vector Laboratories).

    Techniques: Transgenic Assay, Construct, Sequencing, Polymerase Chain Reaction, Southern Blot, Plasmid Preparation, Positive Control, Mouse Assay, Real-time Polymerase Chain Reaction

    Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix DNA microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total RNA for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were

    Journal: Breast Cancer Research : BCR

    Article Title: During hormone depletion or tamoxifen treatment of breast cancer cells the estrogen receptor apoprotein supports cell cycling through the retinoic acid receptor ?1 apoprotein

    doi: 10.1186/bcr2827

    Figure Lengend Snippet: Tamoxifen-insensitivity of RAR expression and the functional categories of target genes of the apo-ER apo-RARα1 axis . (A) Hormone-depleted MCF-7 cells were treated with OH-Tam (100 nM) or vehicle control. Seventy-two hours later, the cell lysates were prepared and analysed by western blot using antibodies specific for RARα, RARβ or RARγ; GAPDH was probed as a loading control. The band intensities are normalized to GAPDH and equalized to a value of 1 for the untreated sample. (B) Real time RT-PCR analysis to confirm the effect of knocking down either ER or RARα on the mRNA levels of representative target genes found by Affymetrix DNA microarray in this study to be regulated by apo-ER and apo-RAR in MCF-7 cells: Cells were transfected with control siRNA, ER siRNA or RARα siRNA and four days later the cells were harvested to extract total RNA for the measurement of the relevant mRNAs; the values were normalized those for GAPDH (control). The P -values for the differences noted in the text were

    Article Snippet: Reverse transcription PCR reactions were performed using 500 ng of total RNA and the high capacity complementary DNA Archive kit (Applied Biosystems) according to the vendor's protocol. cDNAs of RARα1 and RARα2 were amplified by competitive PCR.

    Techniques: Expressing, Functional Assay, Western Blot, Quantitative RT-PCR, Microarray, Transfection