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  • cd9  (Abcam)
    99
    Abcam cd9
    Cd9, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd9/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd9 - by Bioz Stars, 2021-06
    99/100 stars
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    86
    Santa Cruz Biotechnology cd9
    Primary human LECs release EEVs. (a) Transmitted EM (TEM) of human skin LECs in situ (left: antipodoplanin-conjugated gold particles) and in vitro (middle and right). MVBs (middle, unstained) and exocytosis of CD63 + vesicles (right, anti-CD63–conjugated gold particles) into the basolateral stroma ( n ≥ 5). (b) Experimental setup to collect basolateral LEC culture supernatant from the lower chamber for the enrichment of basolateral EEVs (yellow dots). (c) TEM of vesicles of basolateral EEV fractions. Right: Anti-CD63–conjugated gold particles ( n ≥ 21). Bars, 100 nm. (d) Diameters of vesicles in basolateral EEV fractions collected from ss ( n = 21) and TNFα-stimulated LECs ( n = 26). Data are obtained from at least seven TEM images per experiment and from three independent experiments that were pooled for analysis (unpaired two-tailed t test with Welch’s correction). (e) BCA-derived protein concentrations of EEV fractions collected over 24 h from ss and TNFα-stimulated LECs in the absence or presence of 10 µM GW4869 ( n = 3; unpaired two-tailed t test). (f) <t>Anti-CD9</t> immunoblot of EEV fractions from equal volumes of basolateral ss and TNFα-stimulated LEC culture supernatants ( n = 3). (g) Mean absolute vesicle numbers of EEV fractions isolated from 1 ml basolateral LEC culture supernatant of ss and TNFα-stimulated LECs ( n = 3; unpaired two-tailed t test). Left graph, vesicle numbers plotted against diameter size intervals; right graph, vesicle numbers of all sizes. (h) Coomassie blue–stained electrophoresis gel (top) and quantitation of relative protein abundance (as measured by densitometric analyses; bottom) of density gradient centrifugation subfractions of EEV fractions isolated from basolateral culture supernatants of ss and TNFα-stimulated LECs ( n = 3). (i) Anti-CD63–stained immunoblot (top) and quantitation of relative protein abundance (as measured by densitometric analyses; bottom) of density gradient centrifugation subfractions of EEV fractions isolated from basolateral culture supernatants of ss and TNFα-stimulated LECs ( n = 3). Values represent means ± SEM. ns, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01.
    Cd9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd9/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd9 - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    N/A
    This is a recombinant monoclonal antibody
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    N/A
    Rat monoclonal CD9 antibody
      Buy from Supplier

    N/A
    CD9 is a type IV transmembrane glycoprotein with four transmembrane domains CD9 on pre B cells may play a role in cell cell adhesion In addition CD9 may play a
      Buy from Supplier

    Image Search Results


    Primary human LECs release EEVs. (a) Transmitted EM (TEM) of human skin LECs in situ (left: antipodoplanin-conjugated gold particles) and in vitro (middle and right). MVBs (middle, unstained) and exocytosis of CD63 + vesicles (right, anti-CD63–conjugated gold particles) into the basolateral stroma ( n ≥ 5). (b) Experimental setup to collect basolateral LEC culture supernatant from the lower chamber for the enrichment of basolateral EEVs (yellow dots). (c) TEM of vesicles of basolateral EEV fractions. Right: Anti-CD63–conjugated gold particles ( n ≥ 21). Bars, 100 nm. (d) Diameters of vesicles in basolateral EEV fractions collected from ss ( n = 21) and TNFα-stimulated LECs ( n = 26). Data are obtained from at least seven TEM images per experiment and from three independent experiments that were pooled for analysis (unpaired two-tailed t test with Welch’s correction). (e) BCA-derived protein concentrations of EEV fractions collected over 24 h from ss and TNFα-stimulated LECs in the absence or presence of 10 µM GW4869 ( n = 3; unpaired two-tailed t test). (f) Anti-CD9 immunoblot of EEV fractions from equal volumes of basolateral ss and TNFα-stimulated LEC culture supernatants ( n = 3). (g) Mean absolute vesicle numbers of EEV fractions isolated from 1 ml basolateral LEC culture supernatant of ss and TNFα-stimulated LECs ( n = 3; unpaired two-tailed t test). Left graph, vesicle numbers plotted against diameter size intervals; right graph, vesicle numbers of all sizes. (h) Coomassie blue–stained electrophoresis gel (top) and quantitation of relative protein abundance (as measured by densitometric analyses; bottom) of density gradient centrifugation subfractions of EEV fractions isolated from basolateral culture supernatants of ss and TNFα-stimulated LECs ( n = 3). (i) Anti-CD63–stained immunoblot (top) and quantitation of relative protein abundance (as measured by densitometric analyses; bottom) of density gradient centrifugation subfractions of EEV fractions isolated from basolateral culture supernatants of ss and TNFα-stimulated LECs ( n = 3). Values represent means ± SEM. ns, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01.

    Journal: The Journal of Cell Biology

    Article Title: Lymphatic exosomes promote dendritic cell migration along guidance cues

    doi: 10.1083/jcb.201612051

    Figure Lengend Snippet: Primary human LECs release EEVs. (a) Transmitted EM (TEM) of human skin LECs in situ (left: antipodoplanin-conjugated gold particles) and in vitro (middle and right). MVBs (middle, unstained) and exocytosis of CD63 + vesicles (right, anti-CD63–conjugated gold particles) into the basolateral stroma ( n ≥ 5). (b) Experimental setup to collect basolateral LEC culture supernatant from the lower chamber for the enrichment of basolateral EEVs (yellow dots). (c) TEM of vesicles of basolateral EEV fractions. Right: Anti-CD63–conjugated gold particles ( n ≥ 21). Bars, 100 nm. (d) Diameters of vesicles in basolateral EEV fractions collected from ss ( n = 21) and TNFα-stimulated LECs ( n = 26). Data are obtained from at least seven TEM images per experiment and from three independent experiments that were pooled for analysis (unpaired two-tailed t test with Welch’s correction). (e) BCA-derived protein concentrations of EEV fractions collected over 24 h from ss and TNFα-stimulated LECs in the absence or presence of 10 µM GW4869 ( n = 3; unpaired two-tailed t test). (f) Anti-CD9 immunoblot of EEV fractions from equal volumes of basolateral ss and TNFα-stimulated LEC culture supernatants ( n = 3). (g) Mean absolute vesicle numbers of EEV fractions isolated from 1 ml basolateral LEC culture supernatant of ss and TNFα-stimulated LECs ( n = 3; unpaired two-tailed t test). Left graph, vesicle numbers plotted against diameter size intervals; right graph, vesicle numbers of all sizes. (h) Coomassie blue–stained electrophoresis gel (top) and quantitation of relative protein abundance (as measured by densitometric analyses; bottom) of density gradient centrifugation subfractions of EEV fractions isolated from basolateral culture supernatants of ss and TNFα-stimulated LECs ( n = 3). (i) Anti-CD63–stained immunoblot (top) and quantitation of relative protein abundance (as measured by densitometric analyses; bottom) of density gradient centrifugation subfractions of EEV fractions isolated from basolateral culture supernatants of ss and TNFα-stimulated LECs ( n = 3). Values represent means ± SEM. ns, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01.

    Article Snippet: The antibodies against human β-actin, CD9, CD63, and CX3CL1 were purchased from Santa Cruz Biotechnology, Inc.

    Techniques: Transmission Electron Microscopy, In Situ, In Vitro, Two Tailed Test, BIA-KA, Derivative Assay, Isolation, Staining, Electrophoresis, Quantitation Assay, Gradient Centrifugation

    CX3CL1 in EEV fractions promotes transmigration of prostate carcinoma cells. (a) Immunofluorescence of CD9 (red) and podoplanin (green) in the peritumoral stroma of a human prostate carcinoma (fluorescence profiles across respective vessels are plotted above the merged images; x axis, cross-sectional distance; y axis, CD9- or podoplanin-specific mean immunofluorescence intensities; n = 5). Bars, 10 µm. (b) Flow cytometry histogram of unstained (red), APC-conjugated isotype IgG-stained (blue), and APC-conjugated anti-CX3CR1 IgG-stained (green) PC3-ML prostate carcinoma cells. X axis, percentage of maximum; Y axis, mean fluorescence intensity (MFI) of APC-conjugated IgG ( n = 4). (c) Microfluidic adhesion setup (top). EEV fractions were immobilized in microfluidic capillaries, and the adhesions of PC3-ML prostate carcinoma cells were acquired under constant-flow conditions for 2 min. Brightfield images of cancer cells (bottom) that adhered to EEV-free supernatant-coated (left), ss-EEV fraction-coated (middle), or TNFα-EEV fraction-coated (right) microfluidic channels ( n = 3). Bars, 50 µm. (d) Automated quantitation of adherent PC3-ML cell tracks in EEV-free supernatant-coated, ss-EEV fraction-coated, or TNFα-EEV fraction-coated ( n = 3; unpaired two-tailed t test) microfluidic channels. (e) Brightfield images of transmigrated PC3-ML prostate carcinoma cells from the upper cell culture insert into the lower chamber well of a transwell assay. PC3-ML cells were loaded into the upper cell culture inserts after EEV-free supernatants (leftmost), ss-EEV fractions (center left), TNFα-EEV fractions (middle), TNFα-EEV fractions plus anti-CX3CL1 IgG (center right), or TNFα-EEV fractions plus isotype control IgG (rightmost) were loaded into the bottom chamber wells ( n = 3). Bars, 100 µm. (f) Quantitation of transmigrated PC3-ML cells described in d ( n = 3; unpaired two-tailed t test). ns, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01.

    Journal: The Journal of Cell Biology

    Article Title: Lymphatic exosomes promote dendritic cell migration along guidance cues

    doi: 10.1083/jcb.201612051

    Figure Lengend Snippet: CX3CL1 in EEV fractions promotes transmigration of prostate carcinoma cells. (a) Immunofluorescence of CD9 (red) and podoplanin (green) in the peritumoral stroma of a human prostate carcinoma (fluorescence profiles across respective vessels are plotted above the merged images; x axis, cross-sectional distance; y axis, CD9- or podoplanin-specific mean immunofluorescence intensities; n = 5). Bars, 10 µm. (b) Flow cytometry histogram of unstained (red), APC-conjugated isotype IgG-stained (blue), and APC-conjugated anti-CX3CR1 IgG-stained (green) PC3-ML prostate carcinoma cells. X axis, percentage of maximum; Y axis, mean fluorescence intensity (MFI) of APC-conjugated IgG ( n = 4). (c) Microfluidic adhesion setup (top). EEV fractions were immobilized in microfluidic capillaries, and the adhesions of PC3-ML prostate carcinoma cells were acquired under constant-flow conditions for 2 min. Brightfield images of cancer cells (bottom) that adhered to EEV-free supernatant-coated (left), ss-EEV fraction-coated (middle), or TNFα-EEV fraction-coated (right) microfluidic channels ( n = 3). Bars, 50 µm. (d) Automated quantitation of adherent PC3-ML cell tracks in EEV-free supernatant-coated, ss-EEV fraction-coated, or TNFα-EEV fraction-coated ( n = 3; unpaired two-tailed t test) microfluidic channels. (e) Brightfield images of transmigrated PC3-ML prostate carcinoma cells from the upper cell culture insert into the lower chamber well of a transwell assay. PC3-ML cells were loaded into the upper cell culture inserts after EEV-free supernatants (leftmost), ss-EEV fractions (center left), TNFα-EEV fractions (middle), TNFα-EEV fractions plus anti-CX3CL1 IgG (center right), or TNFα-EEV fractions plus isotype control IgG (rightmost) were loaded into the bottom chamber wells ( n = 3). Bars, 100 µm. (f) Quantitation of transmigrated PC3-ML cells described in d ( n = 3; unpaired two-tailed t test). ns, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01.

    Article Snippet: The antibodies against human β-actin, CD9, CD63, and CX3CL1 were purchased from Santa Cruz Biotechnology, Inc.

    Techniques: Transmigration Assay, Immunofluorescence, Fluorescence, Flow Cytometry, Cytometry, Staining, Quantitation Assay, Two Tailed Test, Cell Culture, Transwell Assay

    Immunohistological evidence for exosome accumulation in the perilymphatic stroma of inflamed human kidneys. (a and b) Immunofluorescence of the lymphatic vessel marker podoplanin (green) and the exosome markers CD9 (a, red) or CD63 (b, red) in human renal transplant rejections (fluorescence profiles across respective vessels are plotted beneath merged images; x axis: cross-sectional distance; y axis: CD9-, CD63-, or podoplanin-specific mean immunofluorescence intensities). Cell nuclei are stained with DAPI (blue; n = 14). (c) Immunofluorescence of the lymphatic vessel marker podoplanin (green) and the exosome marker CD9 (red) in normal human kidneys (fluorescence profile across respective vessel is plotted beneath merged images; x axis: cross-sectional distance; y axis: C9- or podoplanin-specific mean immunofluorescence intensities). Cell nuclei are stained with DAPI (blue; n = 12). Bars, 10 µm. (d) Ratios of fluorescence diameters of CD9/podoplanin stainings of lymphatic vessels in normal ( n = 12) and rejecting transplanted human kidneys ( n = 14). Data obtained from at least two lymphatic vessels per patient sample and from six different patients who were pooled for analysis (unpaired two-tailed t test with Welch’s correction). Values represent means ± SEM.

    Journal: The Journal of Cell Biology

    Article Title: Lymphatic exosomes promote dendritic cell migration along guidance cues

    doi: 10.1083/jcb.201612051

    Figure Lengend Snippet: Immunohistological evidence for exosome accumulation in the perilymphatic stroma of inflamed human kidneys. (a and b) Immunofluorescence of the lymphatic vessel marker podoplanin (green) and the exosome markers CD9 (a, red) or CD63 (b, red) in human renal transplant rejections (fluorescence profiles across respective vessels are plotted beneath merged images; x axis: cross-sectional distance; y axis: CD9-, CD63-, or podoplanin-specific mean immunofluorescence intensities). Cell nuclei are stained with DAPI (blue; n = 14). (c) Immunofluorescence of the lymphatic vessel marker podoplanin (green) and the exosome marker CD9 (red) in normal human kidneys (fluorescence profile across respective vessel is plotted beneath merged images; x axis: cross-sectional distance; y axis: C9- or podoplanin-specific mean immunofluorescence intensities). Cell nuclei are stained with DAPI (blue; n = 12). Bars, 10 µm. (d) Ratios of fluorescence diameters of CD9/podoplanin stainings of lymphatic vessels in normal ( n = 12) and rejecting transplanted human kidneys ( n = 14). Data obtained from at least two lymphatic vessels per patient sample and from six different patients who were pooled for analysis (unpaired two-tailed t test with Welch’s correction). Values represent means ± SEM.

    Article Snippet: The antibodies against human β-actin, CD9, CD63, and CX3CL1 were purchased from Santa Cruz Biotechnology, Inc.

    Techniques: Immunofluorescence, Marker, Fluorescence, Staining, Two Tailed Test

    Induction of protrusion formation and enhancement of directional migration by TNFα-EEV fractions is dependent on GPCR signaling and CX3CL1. (a) Quantitation of transmigrated human mature MMDCs from the upper cell culture insert into the lower chamber well of a transwell assay. PTX-treated or untreated MMDCs were loaded together with EEV-free supernatants or TNFα-EEV fractions into the upper cell culture insert ( n = 3; unpaired two-tailed t test). (b) Immunofluorescence of CX3CL1 (green) and ALIX (red; left) or CD9 (red; right) in primary human LECs. Cell nuclei are stained with DAPI (blue; n = 5). Yellow regions of interest were used for Manders colocalization analyses. White region of interest is a zoom. Arrows indicate colocalization of CX3CL1 with either ALIX or CD9. (c) Immunofluorescence of the lymphatic vessel marker podoplanin (green) and CX3CL1 (red) in human renal transplant rejections (fluorescence profiles across the respective vessels are plotted beneath the merged images; x axis: cross-sectional distance; y axis: CX3CL1- or podoplanin-specific mean immunofluorescence intensities; n = 6). Bars, 5 µm. (d) Anti-CX3CL1 immunoblot of ss-EEV fractions and TNFα-EEV fractions probed with an antibody to the C terminus of CX3CL1 ( n = 5). Molecular masses are given in kilodaltons. (e) Flow cytometry contour plot of unstained beads, isotype control IgG-coated beads stained with fluorescent SYTO RNASelect-labeled TNFα-EEV fractions and anti-CX3CL1 IgG-coated beads stained with fluorescent SYTO RNASelect-labeled TNFα-EEV fractions. X axis indicates forward scatter. Y axis indicates mean fluorescence intensity (MFI) of SYTO RNASelect–labeled EEVs ( n = 6). (f) Quantitation of SYTO RNASelect MFI of beads described in e ( n = 6; unpaired two-tailed t test). (g) Quantitation of transmigrated MMDCs from the upper cell culture insert into the lower chamber well of a transwell assay. MMDCs were loaded together with EEV-free supernatants or TNFα-EEV fractions and control IgGs or anti-CX3CL1 IgGs into the upper cell culture insert ( n = 3; unpaired two-tailed t test). (h) Quantitation of transmigrated MMDCs from the upper cell culture insert into the lower chamber well of a transwell assay. MMDCs were loaded together with EEV-free supernatants or TNFα-EEV fractions derived from 5-mispair control morpholino oligonucleotide–treated LECs or CX3CL1-specific morpholino oligonucleotide–treated LECs into the upper cell culture insert ( n = 2; unpaired two-tailed t test). (i) Migration analyses of MMDCs in a 3D collagen matrix migration assay. Cells were exposed to gradients of EEV-free supernatants plus CCL19 ( n = 1,070), TNFα-EEV fractions derived from 5-mispair control morpholino oligonucleotide–treated LECs plus CCL19 ( n = 654) or TNFα-EEV fractions derived from CX3CL1-specific morpholino oligonucleotide–treated LECs plus CCL19 ( n = 958). Red columns indicate chemotactic displacement. Blue columns indicate chemotactic index. Data are obtained from at least 218 cell tracks per experiment and three independent experiments that were pooled for analysis (unpaired two-tailed t test with Welch’s correction). (j) Migration analyses of MMDCs in a confined microenvironment migration assay in the presence of EEV-free supernatants ( n = 1,464), TNFα-EEV fractions derived from 5-mispair control morpholino oligonucleotide–treated LECs ( n = 9,380), or TNFα-EEV fractions derived from CX3CL1-specific morpholino oligonucleotide–treated LECs ( n = 5,588). Red columns indicate circularity of cell shape. Blue columns indicate migratory angle change. Green columns indicate speed of migration. Data are obtained from at least 488 time points and from three independent experiments that were pooled for analysis (unpaired two-tailed t test with Welch’s correction). Values represent means ± SEM. ns, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.

    Journal: The Journal of Cell Biology

    Article Title: Lymphatic exosomes promote dendritic cell migration along guidance cues

    doi: 10.1083/jcb.201612051

    Figure Lengend Snippet: Induction of protrusion formation and enhancement of directional migration by TNFα-EEV fractions is dependent on GPCR signaling and CX3CL1. (a) Quantitation of transmigrated human mature MMDCs from the upper cell culture insert into the lower chamber well of a transwell assay. PTX-treated or untreated MMDCs were loaded together with EEV-free supernatants or TNFα-EEV fractions into the upper cell culture insert ( n = 3; unpaired two-tailed t test). (b) Immunofluorescence of CX3CL1 (green) and ALIX (red; left) or CD9 (red; right) in primary human LECs. Cell nuclei are stained with DAPI (blue; n = 5). Yellow regions of interest were used for Manders colocalization analyses. White region of interest is a zoom. Arrows indicate colocalization of CX3CL1 with either ALIX or CD9. (c) Immunofluorescence of the lymphatic vessel marker podoplanin (green) and CX3CL1 (red) in human renal transplant rejections (fluorescence profiles across the respective vessels are plotted beneath the merged images; x axis: cross-sectional distance; y axis: CX3CL1- or podoplanin-specific mean immunofluorescence intensities; n = 6). Bars, 5 µm. (d) Anti-CX3CL1 immunoblot of ss-EEV fractions and TNFα-EEV fractions probed with an antibody to the C terminus of CX3CL1 ( n = 5). Molecular masses are given in kilodaltons. (e) Flow cytometry contour plot of unstained beads, isotype control IgG-coated beads stained with fluorescent SYTO RNASelect-labeled TNFα-EEV fractions and anti-CX3CL1 IgG-coated beads stained with fluorescent SYTO RNASelect-labeled TNFα-EEV fractions. X axis indicates forward scatter. Y axis indicates mean fluorescence intensity (MFI) of SYTO RNASelect–labeled EEVs ( n = 6). (f) Quantitation of SYTO RNASelect MFI of beads described in e ( n = 6; unpaired two-tailed t test). (g) Quantitation of transmigrated MMDCs from the upper cell culture insert into the lower chamber well of a transwell assay. MMDCs were loaded together with EEV-free supernatants or TNFα-EEV fractions and control IgGs or anti-CX3CL1 IgGs into the upper cell culture insert ( n = 3; unpaired two-tailed t test). (h) Quantitation of transmigrated MMDCs from the upper cell culture insert into the lower chamber well of a transwell assay. MMDCs were loaded together with EEV-free supernatants or TNFα-EEV fractions derived from 5-mispair control morpholino oligonucleotide–treated LECs or CX3CL1-specific morpholino oligonucleotide–treated LECs into the upper cell culture insert ( n = 2; unpaired two-tailed t test). (i) Migration analyses of MMDCs in a 3D collagen matrix migration assay. Cells were exposed to gradients of EEV-free supernatants plus CCL19 ( n = 1,070), TNFα-EEV fractions derived from 5-mispair control morpholino oligonucleotide–treated LECs plus CCL19 ( n = 654) or TNFα-EEV fractions derived from CX3CL1-specific morpholino oligonucleotide–treated LECs plus CCL19 ( n = 958). Red columns indicate chemotactic displacement. Blue columns indicate chemotactic index. Data are obtained from at least 218 cell tracks per experiment and three independent experiments that were pooled for analysis (unpaired two-tailed t test with Welch’s correction). (j) Migration analyses of MMDCs in a confined microenvironment migration assay in the presence of EEV-free supernatants ( n = 1,464), TNFα-EEV fractions derived from 5-mispair control morpholino oligonucleotide–treated LECs ( n = 9,380), or TNFα-EEV fractions derived from CX3CL1-specific morpholino oligonucleotide–treated LECs ( n = 5,588). Red columns indicate circularity of cell shape. Blue columns indicate migratory angle change. Green columns indicate speed of migration. Data are obtained from at least 488 time points and from three independent experiments that were pooled for analysis (unpaired two-tailed t test with Welch’s correction). Values represent means ± SEM. ns, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.

    Article Snippet: The antibodies against human β-actin, CD9, CD63, and CX3CL1 were purchased from Santa Cruz Biotechnology, Inc.

    Techniques: Migration, Quantitation Assay, Cell Culture, Transwell Assay, Two Tailed Test, Immunofluorescence, Staining, Marker, Fluorescence, Flow Cytometry, Cytometry, Labeling, Derivative Assay