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  • 99
    Thermo Fisher superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript ii reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    superscript ii reverse transcriptase - by Bioz Stars, 2021-07
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    99
    TaKaRa sybr premix ex taq ii
    Sybr Premix Ex Taq Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr premix ex taq ii/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr premix ex taq ii - by Bioz Stars, 2021-07
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    86
    Becton Dickinson lsr ii flow cytometer
    Flow <t>cytometry</t> of E. coli challenged with antibiotics. At 0 h, 0.5 h, 2 h, and 5 h time points, viability of E. coli MG1655 challenged with ~1× MBC of ampicillin ( A , B ), ~1× MBC of polymyxin B ( C , D ), ~1× MBC of ciprofloxacin ( E , F ), and ~1× MIC of chloramphenicol ( G , H ) was determined by measuring fluorescence of SYTO 9- and PI-stained cultures using the Optrode ( B , D , F , H ) and a <t>LSR-II</t> flow cytometer ( A , C , E , G ) and by culture-based enumeration ( B , D , F , H ). FCM generated a total cell count, live cell count, dying cell count, and dead cell count ( A , C , E , G ). The dot-dash line represents the limit of quantification for FCM (1 × 10 5 cells/mL). For Optrode measurements, fluorescence intensities were obtained from integrating 505–515 nm for green emissions and 600–610 nm for red emissions. The proportion of live cells in the sample population was determined by the adjusted dye ratio (ADR; B , D , F , H ). The limit of detection for culture-based enumeration is 500 CFU/mL (dashed line). Data presented is from three biological replicates with a line plotted at the median.
    Lsr Ii Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lsr ii flow cytometer/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lsr ii flow cytometer - by Bioz Stars, 2021-07
    86/100 stars
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    PIN 1 recognizes a 33 35 kDa protein doublet corresponding to the apparent molecular mass of the p33 and p35 forms of the cytoplasmic tail of human CD74 a type
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    RayBio Human Immunoquantitative PCR Based CD74 ELISA Kit for cell culture supernatants plasma and serum samples
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    RayBio Human CD74 ELISA Kit for serum plasma and cell culture supernatant samples
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    Recombinant Human CD74 protein with His tag
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    Image Search Results


    Flow cytometry of E. coli challenged with antibiotics. At 0 h, 0.5 h, 2 h, and 5 h time points, viability of E. coli MG1655 challenged with ~1× MBC of ampicillin ( A , B ), ~1× MBC of polymyxin B ( C , D ), ~1× MBC of ciprofloxacin ( E , F ), and ~1× MIC of chloramphenicol ( G , H ) was determined by measuring fluorescence of SYTO 9- and PI-stained cultures using the Optrode ( B , D , F , H ) and a LSR-II flow cytometer ( A , C , E , G ) and by culture-based enumeration ( B , D , F , H ). FCM generated a total cell count, live cell count, dying cell count, and dead cell count ( A , C , E , G ). The dot-dash line represents the limit of quantification for FCM (1 × 10 5 cells/mL). For Optrode measurements, fluorescence intensities were obtained from integrating 505–515 nm for green emissions and 600–610 nm for red emissions. The proportion of live cells in the sample population was determined by the adjusted dye ratio (ADR; B , D , F , H ). The limit of detection for culture-based enumeration is 500 CFU/mL (dashed line). Data presented is from three biological replicates with a line plotted at the median.

    Journal: Microorganisms

    Article Title: Rapid Detection of Escherichia coli Antibiotic Susceptibility Using Live/Dead Spectrometry for Lytic Agents

    doi: 10.3390/microorganisms9050924

    Figure Lengend Snippet: Flow cytometry of E. coli challenged with antibiotics. At 0 h, 0.5 h, 2 h, and 5 h time points, viability of E. coli MG1655 challenged with ~1× MBC of ampicillin ( A , B ), ~1× MBC of polymyxin B ( C , D ), ~1× MBC of ciprofloxacin ( E , F ), and ~1× MIC of chloramphenicol ( G , H ) was determined by measuring fluorescence of SYTO 9- and PI-stained cultures using the Optrode ( B , D , F , H ) and a LSR-II flow cytometer ( A , C , E , G ) and by culture-based enumeration ( B , D , F , H ). FCM generated a total cell count, live cell count, dying cell count, and dead cell count ( A , C , E , G ). The dot-dash line represents the limit of quantification for FCM (1 × 10 5 cells/mL). For Optrode measurements, fluorescence intensities were obtained from integrating 505–515 nm for green emissions and 600–610 nm for red emissions. The proportion of live cells in the sample population was determined by the adjusted dye ratio (ADR; B , D , F , H ). The limit of detection for culture-based enumeration is 500 CFU/mL (dashed line). Data presented is from three biological replicates with a line plotted at the median.

    Article Snippet: Flow cytometry measurements were taken on an LSR-II flow cytometer (Becton Dickinson) with the threshold set to side scatter (SSC) and flow rate set to the lowest possible, 6 µL/minute [ ].

    Techniques: Flow Cytometry, Fluorescence, Staining, Generated, Cell Counting

    Flow cytometry of untreated E. coli and E. coli challenged with isopropanol. At 0 h and 0.25 h time points, viability of E. coli MG1655 challenged with 70% isopropanol ( C , D ) or nothing (untreated) at 37 °C ( A , B ) and 28 °C ( E , F ) was determined by measuring fluorescence of SYTO 9- and PI-stained cultures using the Optrode ( B , D , F ) and a LSR-II flow cytometer ( A , C , E ) and by culture-based enumeration ( B , D , F ). Measurements of untreated cells at 37 °C were taken at 0 h, 0.5 h, 2 h, and 5 h time points to reflect the time points for antibiotic challenge, while for untreated cells at 28 °C, measurements were taken at 0 h and 0.25 h to reflect the isopropanol challenge. FCM generated a total cell count, live cell count, dying cell count, and dead cell count ( A , C , E ). The dot-dash line represents the limit of quantification for FCM (1 × 10 5 cells/mL). For Optrode measurements, fluorescence intensities were obtained from integrating 505–515 nm for green emissions and 600–610 nm for red emissions. The proportion of live cells in the sample population was determined by the adjusted dye ratio (ADR; B , D , F ). The limit of detection for culture-based enumeration is 500 CFU/mL (dashed line). Data presented is from three biological replicates with a line plotted at the median.

    Journal: Microorganisms

    Article Title: Rapid Detection of Escherichia coli Antibiotic Susceptibility Using Live/Dead Spectrometry for Lytic Agents

    doi: 10.3390/microorganisms9050924

    Figure Lengend Snippet: Flow cytometry of untreated E. coli and E. coli challenged with isopropanol. At 0 h and 0.25 h time points, viability of E. coli MG1655 challenged with 70% isopropanol ( C , D ) or nothing (untreated) at 37 °C ( A , B ) and 28 °C ( E , F ) was determined by measuring fluorescence of SYTO 9- and PI-stained cultures using the Optrode ( B , D , F ) and a LSR-II flow cytometer ( A , C , E ) and by culture-based enumeration ( B , D , F ). Measurements of untreated cells at 37 °C were taken at 0 h, 0.5 h, 2 h, and 5 h time points to reflect the time points for antibiotic challenge, while for untreated cells at 28 °C, measurements were taken at 0 h and 0.25 h to reflect the isopropanol challenge. FCM generated a total cell count, live cell count, dying cell count, and dead cell count ( A , C , E ). The dot-dash line represents the limit of quantification for FCM (1 × 10 5 cells/mL). For Optrode measurements, fluorescence intensities were obtained from integrating 505–515 nm for green emissions and 600–610 nm for red emissions. The proportion of live cells in the sample population was determined by the adjusted dye ratio (ADR; B , D , F ). The limit of detection for culture-based enumeration is 500 CFU/mL (dashed line). Data presented is from three biological replicates with a line plotted at the median.

    Article Snippet: Flow cytometry measurements were taken on an LSR-II flow cytometer (Becton Dickinson) with the threshold set to side scatter (SSC) and flow rate set to the lowest possible, 6 µL/minute [ ].

    Techniques: Flow Cytometry, Fluorescence, Staining, Generated, Cell Counting