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All (9)
Becton Dickinson
Cytek Development
Thermo Fisher
Revvity
SouthernBiotech
Abeomics
BD Biosciences
BioLegend
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About 37 products / 100 snippets / 25 images

80 / 100
Bioz Stars
Anti–CD45.1-Cy7
Becton Dickinson / BD Biosciences  
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Techniques
 
Article Snippets
".. anti–CD4-PE, anti–CD4-FITC, anti–CD8aFITC, anti–CD45-allophycocyanin, anti–IFN-g-PE, and anti–CD45.1-Cy7 were purchased from BD Biosciences (San Diego, CA)."  (see more)
86 / 100
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Anti CD45.1-APC-Cy 7
Becton Dickinson / BD Biosciences  
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Article Snippets
".. 6 BM cells were stained at a ratio of 1:50 with the following antibodies: CD45.1-APC-Cy 7 (BD Biosciences; cat. no. 560579) for murine cells, CD3-APC (BD Biosciences; cat. no. 553066) .."  (see more)
21+  more images
(a) TRAF6 mRNA in CD34 + BM cells from low-risk MDS patients (n = 36) and age-matched healthy controls (HC; n = 7) (left), and LSK cells from Vav -TRAF6 (n = 6) and WT FVB/NJ (n = 4) mice (primers are designed to measure both endogenous mouse Traf6 and ectopic human TRAF6). P = 0.02. ( b ) Disease-free survival of Vav -TRAF6 (n = 60) and WT FVB/NJ mice (n = 40) *, P = 0.009. ( c ) Complete blood counts for WT FVB/NJ (n = 33) and moribund Vav -TRAF6 (n = 18) mice. *. P < 0.01. ( d ) BM neutrophil dysplasia in age-matched WT (n = 3) and Vav -TRAF6 (n = 5) FVB/NJ mice. At least 100 cells were examined for each mouse. *, P = 0.04. ( e ) BM mononuclear cell counts from WT (n = 3) and Vav -TRAF6 FVB/NJ mice (n = 4). *, P = 0.004. ( f ) Representative Wright-Giemsa-stained peripheral blood smears (PB) and bone marrow cytospins (BM) from WT (FVB/NJ) and 2 moribund Vav -TRAF6 mice. The red arrow indicates dysplastic myeloid cells with Pseudo-Pelger Hüet anomaly. Scale bars, 10 µm (top) and 7.5 µm (bottom) ( g ) Number of long-term hematopoietic stem cells (LT-HSC, P = 0.0056), short-term HSC (ST-HSC, P = 0.0011), multipotent progenitors (MPP, P = 0.014), common myeloid progenitors (CMP), megakaryocyte-erythroid progenitor (MEP), and granulocyte-monocytic progenitor (GMP) in the BM of WT (C57Bl/6) (n = 4) and Vav -TRAF6 (n = 4) ~6 month old mice. ( h ) Colony numbers were determined after primary and secondary plating LSK BM cells from WT (FVB/NJ; n = 3) and Vav -TRAF6 (n = 3) mice in methylcellulose. *, P < 0.001. ( i ) FACS analysis of WT (C57Bl/6) or Vav -TRAF6 donor-derived <t>(CD45.2</t> + ) and <t>competitor-derived</t> <t>(CD45.1</t> + ) mononuclear cells from peripheral blood 12 weeks post competitive transplantation (left panel). Proportion of myeloid (CD11b + ) and lymphoid (CD3 + and B220 + ) cells within the donor-derived compartment (gated on CD45.2 + ) after competitive transplantation with either BM mononuclear cells (MNC, left) or LT-HSC (right). (n = 8 mice per genotype) (right panel). *, P < 0.05. ( j ) Colony formation in methylcellulose after serial replating of GFP-sorted Lin − BM cells (C57Bl/6) transduced with retroviral vectors (MSCV-IRES-GFP) encoding WT (n = 3) or an E3 ligase-mutant TRAF6 (TRAF6 C70A , n = 3). *, P < 0.01. ( k ) Proportion of donor-derived (gated on CD45.2 + ;GFP + ) peripheral blood myeloid (Cd11b + ) and lymphoid (CD3 + and B220 + ) cells were examined by FACS for competitively transplanted GFP-sorted Lin − BM cells (C57Bl/6) transduced with retroviral vectors encoding WT (n = 5) or an E3 ligase-mutant TRAF6 (TRAF6 C70A , n = 10) co-expressing GFP (MSCV-IRES-GFP). *, P < 0.05. Data are from three experiments ( a, c–g ; means and s.e.m.) or from two experiments ( h–k ; means and s.e.m.).
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(A) Flow cytometric gating strategy. Splenic IL-4-GFP+CD4+ T cells (CD3+CD4+GFPhigh) were analyzed using PD-1 and CXCR5 markers to detect IL-4-GFP+ TFH (PD-1highCXCR5high) and IL-4-GFP+ non-TFH (Th2) cells (PD-1lowCXCR5low). (B) TFH and Th2 cells were analyzed by flow cytometry in unimmunized mice (−) or in immunized mice on day 7 post-immunization with OVA/alum (+). Representative flow cytometric scatter plots (left) and quantification (right) of the frequency of IL-4-GFP+ TFH and IL-4-GFP+ Th2 cells among splenic IL-4-GFP+CD4+ T cells. Ratio between IL-4-GFP+ TFH and IL-4-GFP+ Th2 cells within GFPhighCD4+ T cells was also calculated. (C, D) Reconstitution of CD4+ T cells and TFH in spleens of irradiated Rag2−/− recipient mice engrafted with a mixture containing equal numbers of <t>Prkd2+/+</t> <t>(CD45.1+)</t> and Prkd2−/− (CD45.2+) bone marrow cells (C) or naïve CD4+ T cells (D). Representative flow cytometric scatter plots (left) and quantification (right) of the frequency of TFH cells (CD3+CD4+CXCR5highPD-1high) derived from each donor among CD4+ T cells (lower graphs). Quantification of the frequency of donor-derived CD4+ T cells (CD3+CD4+) in the spleen is also shown (upper graphs). Data are representative of three independent experiments with at least 5 mice/genotype (mean ± SD); P values were determined by unpaired Student’s t test (NS: not significant, **P < 0.01, and ***P < 0.001).
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A) WT mice were infected with LCMV ARM or Cl 13, splenocytes were isolated at day 10 pi and stained with anti-CD8, Db/GP33-41 tetramers and anti-p-Smad-2/3 mAb. p-Smad-2/3 levels in Db/GP33-41 tetramer+CD8+ T cells were determined by FACS. Bar graphs indicate the average mean fluorescence intensity (MFI) ± standard deviation (sd) of 4 mice per group. Histograms depict one representative mouse per group. Thin line, Isotype control. B-D) Donor <t>CD45.2+</t> P14 TCR transgenic CD8+ T cells were transferred into <t>WT</t> <t>CD45.1+</t> recipients, hosts were infected with LCMV ARM or Cl 13, and pooled P14 cells FACS-purified at day 5 or 8-10 pi and processed for immunoblot blot. B) Sketch of experimental design. c) Levels of p-Smad-2, total Smad-2 in P14 cells. D) Levels of TGF-β1 in P14 cells. Phospholipase-γ (PLC-γ) was used as loading control. E-H) WT (black circles or bars) or dnTGFBRII (white squares or bars) mice were infected with LCMV Cl 13. WT-ARM infected mice were processed as controls (striped bars). Virus-specific CD8+ T cells from blood (E) or spleen (F-H) were stained with H2Db/NP396-404, Db/GP33-41 and/or Db/GP276-286 tetramers. E) Log-scaled plots showing average frequencies ± sd of tetramer+CD8+ blood cells at the indicated times pi. F) Dot plots displaying tetramer staining profiles in splenocytes from a representative mouse per group at day 9 pi. Numbers indicate % tetramer+CD8+ cells in the respective gate. G-H) Total numbers of tetramer+CD8+ cells per spleen at day 9 (G) or days 1 through 9 (H) pi. Note that numbers of tetramer+ T cells were similar to background levels at day 1 and 3 pi. All results are representative of two or three independent experiments with three to four mice per group each. (LCMV ARM vs Cl 13 or WT vs dnTGFBRII as indicated, *p<0.05, **p<0.005 and ***p<0.0005)
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93 / 100
Bioz Stars
CD45.1 APC-Cy7
Becton Dickinson / BD Biosciences  
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Article Snippets
".. eBioscience), active Casp3 (C92-605, BD Biosciences), CD45 BUV395 (30-F11, BD Biosciences), CD45.1 APC-Cy7 (A20, BD Biosciences), CD45.2 APC- eF780 (104, eBioscience), CD45.2 BV711 (104, BD Biosciences), CD45.2 .."  (see more)
80 / 100
Bioz Stars
CD45.1 PE-Cy7
Cytek Biosciences / Tonbo Biosciences  
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Article Snippets
"From Tonbo Biosciences: CD45.1 PE-Cy7 (A20), CD115 PE (AFS98), CD11c APC-Cy7 (N418)."  (see more)
80 / 100
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CD45.1-PE Cy7
Cytek Biosciences / Tonbo Biosciences  
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Article Snippets
"CD45.1-PE Cy7 , Tonbo , 60–0453, RRID:AB_2621850 , ."  (see more)
VIR
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93 / 100
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CD45.1 PE-Cy7
Thermo Fisher Scientific / eBioscience  
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Techniques
 
Article Snippets
"Cells were washed with PBS and stained for 15 min in PBS + 2% FBS with CD3e FITC, CD45.1 PE-Cy7, CD8 APC-e780, and CD4 Pacific blue antibodies (eBioscience)."  (see more)
80 / 100
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CD45.1-APC-Cy7
Cytek Biosciences / Tonbo Biosciences  
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Article Snippets
".. CD45.1-FITC (Tonbo Biosciences; 35-0453-U500), CD45.1-PE (Biolegend; 110708), CD45.1-APC (Biolegend; 110714), CD45.1-APC-Cy7 (Tonbo Biosciences; 25-0453-U100), CD45.2-PE-Cy7 (Biolegend; 109830), CD45.2-PerCP-Cy5.5 (Tonbo Biosciences; 65-0454-U100), CD45.2-PE (Tonbo .."  (see more)
80 / 100
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CD45.1-APC-Cy7
Cytek Biosciences / Tonbo Biosciences  
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Techniques
 
Article Snippets
"Adoptively transferred cells were isolated by labeling CD45.1+ cells with CD45.1-APC-Cy7 (Tonbo Biosciences A20) and immunomagnetic enrichment using antiAPC beads (Miltenyi #130-090-855) followed by flow .."  (see more)
94 / 100
Bioz Stars
CD45.1 PE Cy7
Revvity / BioLegend  
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Techniques
 
Article Snippets
".. CD8 PE (53–6.7), CD45.1 PE (A20), CD45.2 PE .... CD8 allophycocyanin (53–6.7) (Biolegend), KLRG1 allophycocyanin (2F1), .."  (see more)
92 / 100
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APC-Cy7 CD45.1
Becton Dickinson  
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Techniques
 
Article Snippets
".. 450 CD45.1 Monoclonal Antibody (A20, Fisher Scientific), PE Mouse Anti-Mouse CD45.1 (A20, BD), APC-Cy7 CD45.1 (A20, BD), PE Mouse Anti-Mouse CD45.2 (104, BD), APC Mouse anti-Mouse CD45.2 (104, BD), BV605 .."  (see more)
80 / 100
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CD45.1 A20 Pe-Cy7
Becton Dickinson / Pharmingen  
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Article Snippets
".. AF647 or BV785, CD40 3/23 BV711, CD80 16-10A1 PE, CD86 GL1 APC, and CD45.1 A20 Pe-Cy7 were all purchased from BD Pharmigen as well."  (see more)
80 / 100
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PE-Cy7 CD45.1 (A20)
Cytek Biosciences / Tonbo Biosciences  
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Article Snippets
"PE-Cy7 CD45.1 (A20) , Tonbo , Cat# 60-0453-U100; RRID: AB_2621850."  (see more)
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