Journal: Nature Communications
Article Title: Extravascular gelation shrinkage-derived internal stress enables tumor starvation therapy with suppressed metastasis and recurrence
Figure Lengend Snippet: In vivo evaluations on intratumoral vascular narrowing on PANC-1 xenografted pancreatic tumors. a CDFI images of PANC-1 xenografted pancreatic tumors implanted on nude mice that were captured at three time points, i.e., pre-, post- and 5 day post-treatment with Laser+hydrogel–GNR (2). b , c In vivo animal fluorescence images ( b ) and quantitative signal intensities ( c ) of PANC-1 xenografted tumor after corresponding treatments in control (i.e., Laser+GNR) and treated (i.e., Laser+hydrogel–GNR (2)) groups and subsequent injections of different FITC-labeled dextrans with varied sizes (i.e., 70, 20, and 4 K), wherein green arrows and dotted ellipses indicate the tumor. Data are expressed as mean ± SD ( n = 3). d , e Time-dependent ultrasonic images ( d ) and average acoustic intensities ( e ) of PANC-1 xenografted tumor before and after corresponding treatments in control and treated groups, wherein the 1st i.v. injection of Sonovue TM microbubbles were carried out, and after 30–90 min, the corresponding treatments in two groups, i.e., control (Laser+GNR) and treated (Laser+hydrogel–GNR (2)), were implemented, followed by the 2nd i.v. injection of Sonovue TM microbubbles; and the ultrasonic images were captured at three time points, i.e., pre-, post-, and 40–120 s post- each i.v. injection of Sonovue TM microbubbles, and red dotted ellipses indicate the tumor. Data are expressed as mean ± SD ( n = 3). f , g Matured ( f ) and budding ( g ) blood vessels of PANC-1 xenografted tumors after corresponding treatments in control and treated groups, and they were stained by FITC-labeled CD34 immunofluorescence ( f ) and Cy3-labeled CD31 immunofluorescence ( g ), respectively, scale bar: 200 μm. Yellow arrows indicate the intratumoral blood vessels. ( h ) In vivo animal fluorescence imaging of nude mice-bearing PANC-1 xenografted tumor at three time points, i.e., pre-, 30 s post- and 50 s post-i.t. injection of Cy3-labeled dextrans-grafted hydrogels, wherein Cy3-labeled dextran (size: 70 K) instead of GNRs was used. Statistical significances were obtained using unpaired student's t test, ** P
Article Snippet: In immunohistochemical staining, Anti-mouse PCNA, Abcam, cat. no. ab29; Anti-CD34 antibody, Abcam, cat. no. ab8158; Hematoxylin and Eosin Staining Kit, Beyotime Biotechnology, cat. no. C0105.
Techniques: In Vivo, Mouse Assay, Fluorescence, Labeling, Injection, Staining, Immunofluorescence, Imaging