Journal: Stem Cell Research & Therapy
Article Title: Heme oxygenase-1-modified bone marrow mesenchymal stem cells combined with normothermic machine perfusion to protect donation after circulatory death liver grafts
Figure Lengend Snippet: Characteristics of HO-1/BMMSCs in vitro and detection of HO-1 expression. a HO-1/BMMSCs were adherent and displayed long spindle-shaped morphology. b HO-1/BMMSCs showed osteogenic differentiation in vitro, as indicated by the calcareous deposits stained black with von Kossa staining. c Adipogenic differentiation of HO-1/BMMSCs, as indicated by oil red O-stained fat cells. d – f Surface biomarker identification. Results showed that 100.0, 99.8, and 99.4% of the cells were positive for CD29, CD90, and RT1A, respectively, and 100.0, 99.8, and 99.4% of the cells were negative for CD34, CD45, and RT1B, respectively. The molecular biological characteristics of HO-1/BMMSCs were not changed. g HO-1 expression in BMMSCs was identified by red fluorescence, and its intensity was weak. h HO-1 expression in HO-1/BMMSCs. The red fluorescence intensity was significantly higher in HO-1/BMMSCs than that in BMMSCs. i Western blotting and qRT-PCR results confirmed that HO-1 expression in HO-1/BMMSCs was significantly higher than that in BMMSCs
Article Snippet: Antibodies against CD29, CD34, CD45, CD90, RT1A, and RT1B (BioLegend, San Diego, CA, USA) were used for phenotype identification by flow cytometry.
Techniques: In Vitro, Expressing, Staining, Biomarker Assay, Fluorescence, Western Blot, Quantitative RT-PCR