cd29 Search Results


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  • 92
    Becton Dickinson cd29
    Single cell RNA sequencing of HNK1 - CD45-CD31 - CXCR4 + <t>CD29</t> + CD56 dim cells. (A) Schematic of differentiating hiPSCs, sorting of iMPCs, and scRNAseq. (B) UMAP visualization plots of the control (2,051 cells) and FOP (5,306 cells) samples. Each dot represents one cell colored by cluster identification. The myogenic cell type was assigned according to expression of a combination of marker genes ( Figure 4-figure supplement 1 ). (C) UMAP of cells combined from both control and FOP samples. (D) Feature expression plots showing the localization of cells expressing myogenic markers, mesenchymal, and neural cell marker. (E) Dot plot displaying expression genes associated with myogenesis, neurogenesis and mesenchymal markers. (F) Proportion of cells per cluster for each sample.
    Cd29, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 3030 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cd29
    Characterization of bone marrow-derived MSCs. Notes: ( A ) Microscopy images of BMSCs phenotype on day 14 (i), osteogenesis and alkaline phosphate activity staining (ii), and adipogenesis and Oil Red O (iii). Magnification 100×. ( B ) Immunophenotypic analysis of cultured BMSCs with monoclonal antibodies. Flow cytometry analysis results showed that the cells were positive for MSC markers <t>CD29,</t> CD44, CD90, CD105, and negative for markers CD34 and CD45. Scale bar: 200 µm. Abbreviations: BMSCs, bone marrow-derived mesenchymal stem cells; CD, cluster of differentiation; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    Cd29, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 872 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti cd29
    Characterization of bone marrow-derived MSCs. Notes: ( A ) Microscopy images of BMSCs phenotype on day 14 (i), osteogenesis and alkaline phosphate activity staining (ii), and adipogenesis and Oil Red O (iii). Magnification 100×. ( B ) Immunophenotypic analysis of cultured BMSCs with monoclonal antibodies. Flow cytometry analysis results showed that the cells were positive for MSC markers <t>CD29,</t> CD44, CD90, CD105, and negative for markers CD34 and CD45. Scale bar: 200 µm. Abbreviations: BMSCs, bone marrow-derived mesenchymal stem cells; CD, cluster of differentiation; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    Anti Cd29, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 96/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Becton Dickinson cd29 pe
    Phenotypic analysis of fetal liver MSC. (A). The expression of hepatocyte-specific markers such as ALB, CK8, CK18, CK19, c-Met and Hep Ag in hFLMSC was examined by immunocytochemistry. First row: negative controls, anti-goat and -mouse secondary antibodies. ALB staining was not detected in hFLMSC. Cells expressing CK8 (brown staining) were detected. Magnification 40 X. Second row: expression of CK18 (brown staining) was detected. A few cells expressed c-Met. hFLMSC did not express CK19 and Hep Ag. Magnification 40 X. (B) hFLMSC were characterized with regard to expression of CD44, CD 106, <t>CD29,</t> CD45, CD73, CD 166, CD54 and CD34 using flow cytometry. First row: hFLMSC showed strong expression of CD44 and weak expression of CD29. Expression of CD 106 and CD45 was not observed. Second row: expression of CD73, CD 166 and CD54 was strong on hFLMSC, while expression of CD34 was not detected.
    Cd29 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cd29  (Abcam)
    96
    Abcam cd29
    Phenotypic analysis of fetal liver MSC. (A). The expression of hepatocyte-specific markers such as ALB, CK8, CK18, CK19, c-Met and Hep Ag in hFLMSC was examined by immunocytochemistry. First row: negative controls, anti-goat and -mouse secondary antibodies. ALB staining was not detected in hFLMSC. Cells expressing CK8 (brown staining) were detected. Magnification 40 X. Second row: expression of CK18 (brown staining) was detected. A few cells expressed c-Met. hFLMSC did not express CK19 and Hep Ag. Magnification 40 X. (B) hFLMSC were characterized with regard to expression of CD44, CD 106, <t>CD29,</t> CD45, CD73, CD 166, CD54 and CD34 using flow cytometry. First row: hFLMSC showed strong expression of CD44 and weak expression of CD29. Expression of CD 106 and CD45 was not observed. Second row: expression of CD73, CD 166 and CD54 was strong on hFLMSC, while expression of CD34 was not detected.
    Cd29, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore cd29
    Characteristics of MSCs/BM. Cells were stained with the CD45, CD90 and <t>CD29</t> antibodies and analyzed by flow cytometry. A) BM-MSCs are shown as a dot plot. B) The expression levels of CD45-ve, C) CD90 + ve and D) CD29 + ve of BM-MSCs are presented as a histogram. MSCs/BM, bone marrow-derived mesenchymal stem cells.
    Cd29, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher cd29 integrin beta 1 monoclonal antibody
    Characteristics of MSCs/BM. Cells were stained with the CD45, CD90 and <t>CD29</t> antibodies and analyzed by flow cytometry. A) BM-MSCs are shown as a dot plot. B) The expression levels of CD45-ve, C) CD90 + ve and D) CD29 + ve of BM-MSCs are presented as a histogram. MSCs/BM, bone marrow-derived mesenchymal stem cells.
    Cd29 Integrin Beta 1 Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    BioLegend cd29
    Characterization of Murine Sca-1 + <t>CD29</t> + CD45 − CD11b − BMSCs
    Cd29, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 598 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    BioLegend pe anti human cd29
    Comparison of MSCs derived from 2D-hESCs and 3D-hESCs. a The morphology of 2D- and 3D-hESC-MSCs at different stages of differentiation. b Flow cytometry analysis revealed specific MSC surface markers (CD44, <t>CD29,</t> and CD105) with negative controls (CD34, CD19, and CD45) in 2D- and 3D-hESC-MSCs. c Immunostaining of differentiated 3D-hESC-MSCs expressing an adipocyte marker (FABP-4), osteocytes maker (osteocalcin), and chondrocytes marker (aggrecan)
    Pe Anti Human Cd29, supplied by BioLegend, used in various techniques. Bioz Stars score: 98/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    BioLegend pe anti mouse rat cd29
    Comparison of MSCs derived from 2D-hESCs and 3D-hESCs. a The morphology of 2D- and 3D-hESC-MSCs at different stages of differentiation. b Flow cytometry analysis revealed specific MSC surface markers (CD44, <t>CD29,</t> and CD105) with negative controls (CD34, CD19, and CD45) in 2D- and 3D-hESC-MSCs. c Immunostaining of differentiated 3D-hESC-MSCs expressing an adipocyte marker (FABP-4), osteocytes maker (osteocalcin), and chondrocytes marker (aggrecan)
    Pe Anti Mouse Rat Cd29, supplied by BioLegend, used in various techniques. Bioz Stars score: 97/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Becton Dickinson anti cd29 pe
    Culture, isolation and identification of human DPSCs. (a, b) Primary cultured DPSCs. (c–f) Odontogenic differentiation of DPSCs was assessed by ALP and Alizarin Red S staining. (g) Flow cytometry was used to detect the surface markers of DPSCs. Cells were incubated with fluorescence-conjugated antibodies against <t>CD29,</t> CD34, CD44, and CD45. Isotype-identical antibodies served as controls. Analysis of surface antigens in DPSCs by flow cytometry indicated that the cells were positive for CD29 and CD44, while CD34 and CD45 were negative (red line).
    Anti Cd29 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher β1 integrin
    Culture, isolation and identification of human DPSCs. (a, b) Primary cultured DPSCs. (c–f) Odontogenic differentiation of DPSCs was assessed by ALP and Alizarin Red S staining. (g) Flow cytometry was used to detect the surface markers of DPSCs. Cells were incubated with fluorescence-conjugated antibodies against <t>CD29,</t> CD34, CD44, and CD45. Isotype-identical antibodies served as controls. Analysis of surface antigens in DPSCs by flow cytometry indicated that the cells were positive for CD29 and CD44, while CD34 and CD45 were negative (red line).
    β1 Integrin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson antibodies against cd29
    Culture, isolation and identification of human DPSCs. (a, b) Primary cultured DPSCs. (c–f) Odontogenic differentiation of DPSCs was assessed by ALP and Alizarin Red S staining. (g) Flow cytometry was used to detect the surface markers of DPSCs. Cells were incubated with fluorescence-conjugated antibodies against <t>CD29,</t> CD34, CD44, and CD45. Isotype-identical antibodies served as controls. Analysis of surface antigens in DPSCs by flow cytometry indicated that the cells were positive for CD29 and CD44, while CD34 and CD45 were negative (red line).
    Antibodies Against Cd29, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    BioLegend anti cd29
    Bone mesenchymal stem cells (BMSCs) identification and efficiency of transduction. (A) Flow cytometry analysis for <t>CD29,</t> CD45, CD90 and CD105. (B) The immunofluorescent micrograph of BMSCs infected with hypoxia-inducible factor 1α (Hif-1α)-GFP lentiviral vector, Hif-1α siRNA or their negative controls (NCs). Scale bar = 200 μm. (C) Efficiency of gene transduction was detected by flow cytometry analysis.
    Anti Cd29, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend fitc anti mouse rat cd29
    Bone mesenchymal stem cells (BMSCs) identification and efficiency of transduction. (A) Flow cytometry analysis for <t>CD29,</t> CD45, CD90 and CD105. (B) The immunofluorescent micrograph of BMSCs infected with hypoxia-inducible factor 1α (Hif-1α)-GFP lentiviral vector, Hif-1α siRNA or their negative controls (NCs). Scale bar = 200 μm. (C) Efficiency of gene transduction was detected by flow cytometry analysis.
    Fitc Anti Mouse Rat Cd29, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Miltenyi Biotec cd29
    Characterization of adipose derived stem cells (ADSC). a ADSC were analyzed for cell surface markers in passage 6 (P6) and passage 11 (P11). Over 90% of cells were positive for CD90 and <t>CD29</t> and negative for CD45 and CD11b/c in both passages. Paired t-test between P6 and P11 showed no differences in expression of surface markers. ADSC in passage 4 were differentiated into adipocytes ( b ), osteocytes ( c ), and chondrocytes ( d ). Lipid vacuoles were visualized with oil red O staining ( b ), calcium deposits were stained with Alizarin Red S ( c ), and proteoglycans of chondrogenic pellets were detected by Alcian blue staining ( d ). Inserts represent ADSC, cultured in proliferation medium as negative controls
    Cd29, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend cd29 apc
    Characterization of adipose derived stem cells (ADSC). a ADSC were analyzed for cell surface markers in passage 6 (P6) and passage 11 (P11). Over 90% of cells were positive for CD90 and <t>CD29</t> and negative for CD45 and CD11b/c in both passages. Paired t-test between P6 and P11 showed no differences in expression of surface markers. ADSC in passage 4 were differentiated into adipocytes ( b ), osteocytes ( c ), and chondrocytes ( d ). Lipid vacuoles were visualized with oil red O staining ( b ), calcium deposits were stained with Alizarin Red S ( c ), and proteoglycans of chondrogenic pellets were detected by Alcian blue staining ( d ). Inserts represent ADSC, cultured in proliferation medium as negative controls
    Cd29 Apc, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson cd29 fitc
    Characterization of adipose derived stem cells (ADSC). a ADSC were analyzed for cell surface markers in passage 6 (P6) and passage 11 (P11). Over 90% of cells were positive for CD90 and <t>CD29</t> and negative for CD45 and CD11b/c in both passages. Paired t-test between P6 and P11 showed no differences in expression of surface markers. ADSC in passage 4 were differentiated into adipocytes ( b ), osteocytes ( c ), and chondrocytes ( d ). Lipid vacuoles were visualized with oil red O staining ( b ), calcium deposits were stained with Alizarin Red S ( c ), and proteoglycans of chondrogenic pellets were detected by Alcian blue staining ( d ). Inserts represent ADSC, cultured in proliferation medium as negative controls
    Cd29 Fitc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    BioLegend alexa fluor 488 anti human cd29
    Characterization of adipose derived stem cells (ADSC). a ADSC were analyzed for cell surface markers in passage 6 (P6) and passage 11 (P11). Over 90% of cells were positive for CD90 and <t>CD29</t> and negative for CD45 and CD11b/c in both passages. Paired t-test between P6 and P11 showed no differences in expression of surface markers. ADSC in passage 4 were differentiated into adipocytes ( b ), osteocytes ( c ), and chondrocytes ( d ). Lipid vacuoles were visualized with oil red O staining ( b ), calcium deposits were stained with Alizarin Red S ( c ), and proteoglycans of chondrogenic pellets were detected by Alcian blue staining ( d ). Inserts represent ADSC, cultured in proliferation medium as negative controls
    Alexa Fluor 488 Anti Human Cd29, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad cd29
    Surface marker expression of in-vitro aging MSCs. MSCs were cultivated with either DMEM-based ( a ) or αMEM-based ( b ) expansion media and were analyzed by flow cytometry for the expression of specific MSC positive (Stro-1, <t>CD29,</t> CD44, CD73, CD90, CD105, CD106, CD 146) and negative (CD34, CD45) surface antigens. n = 4–6; * p
    Cd29, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    R&D Systems human integrin beta 1 cd29 antibody
    Surface marker expression of in-vitro aging MSCs. MSCs were cultivated with either DMEM-based ( a ) or αMEM-based ( b ) expansion media and were analyzed by flow cytometry for the expression of specific MSC positive (Stro-1, <t>CD29,</t> CD44, CD73, CD90, CD105, CD106, CD 146) and negative (CD34, CD45) surface antigens. n = 4–6; * p
    Human Integrin Beta 1 Cd29 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Affinity purified rabbit polyclonal Itgb1 antibody
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    Purified recombinant ITGB1 protein His tag
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    A synthetic peptide for use as a blocking control in assays to test for specificity of Itgb1 antibody catalog no 70R 9607
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    Image Search Results


    Single cell RNA sequencing of HNK1 - CD45-CD31 - CXCR4 + CD29 + CD56 dim cells. (A) Schematic of differentiating hiPSCs, sorting of iMPCs, and scRNAseq. (B) UMAP visualization plots of the control (2,051 cells) and FOP (5,306 cells) samples. Each dot represents one cell colored by cluster identification. The myogenic cell type was assigned according to expression of a combination of marker genes ( Figure 4-figure supplement 1 ). (C) UMAP of cells combined from both control and FOP samples. (D) Feature expression plots showing the localization of cells expressing myogenic markers, mesenchymal, and neural cell marker. (E) Dot plot displaying expression genes associated with myogenesis, neurogenesis and mesenchymal markers. (F) Proportion of cells per cluster for each sample.

    Journal: bioRxiv

    Article Title: ACVR1R206H increases osteogenic/ECM gene expression and impairs myofiber formation in human skeletal muscle stem cells

    doi: 10.1101/2021.01.18.427082

    Figure Lengend Snippet: Single cell RNA sequencing of HNK1 - CD45-CD31 - CXCR4 + CD29 + CD56 dim cells. (A) Schematic of differentiating hiPSCs, sorting of iMPCs, and scRNAseq. (B) UMAP visualization plots of the control (2,051 cells) and FOP (5,306 cells) samples. Each dot represents one cell colored by cluster identification. The myogenic cell type was assigned according to expression of a combination of marker genes ( Figure 4-figure supplement 1 ). (C) UMAP of cells combined from both control and FOP samples. (D) Feature expression plots showing the localization of cells expressing myogenic markers, mesenchymal, and neural cell marker. (E) Dot plot displaying expression genes associated with myogenesis, neurogenesis and mesenchymal markers. (F) Proportion of cells per cluster for each sample.

    Article Snippet: Fixed cells were first incubated with primary antibodies (PAX7 (DSHB), CD29 (BD Biosciences) and CD56 (BD Biosciences) following by secondary antibodies (Alexa350-conjugated goat anti-mouse IgG, Alexa488-conjugated goat anti-rat IgG and Alexa546-conjugated donkey anti-goat IgG, Life Technologies).

    Techniques: RNA Sequencing Assay, Expressing, Marker

    Differentiated cells types in the myogenic differentiation varies between cell lines, sorted cells express PAX7 and can differentiate into myotubes. Related to Figure 3 . (A) Representative flow cytometry profiles of negative (CD45 and CD31) and positive (CD29 and CD56) markers used to purify muscle stem cells. (B) Quantification of the percentage of cells positive for CD45, CD31, CD29, and CD56 at day 20. (n=3 biological replicates and n=3 technical replicates). Error bar represent mean and SD. (C) FACS analysis of the myogenic differentiation at day 50. Muscle stem cells stained for PAX7, CD29, and CD56. (D) Representative immunofluorescence staining for MHC showing sorted muscle stem cells after being cultured in differentiation media for 7 days (scale bar, 200µm).

    Journal: bioRxiv

    Article Title: ACVR1R206H increases osteogenic/ECM gene expression and impairs myofiber formation in human skeletal muscle stem cells

    doi: 10.1101/2021.01.18.427082

    Figure Lengend Snippet: Differentiated cells types in the myogenic differentiation varies between cell lines, sorted cells express PAX7 and can differentiate into myotubes. Related to Figure 3 . (A) Representative flow cytometry profiles of negative (CD45 and CD31) and positive (CD29 and CD56) markers used to purify muscle stem cells. (B) Quantification of the percentage of cells positive for CD45, CD31, CD29, and CD56 at day 20. (n=3 biological replicates and n=3 technical replicates). Error bar represent mean and SD. (C) FACS analysis of the myogenic differentiation at day 50. Muscle stem cells stained for PAX7, CD29, and CD56. (D) Representative immunofluorescence staining for MHC showing sorted muscle stem cells after being cultured in differentiation media for 7 days (scale bar, 200µm).

    Article Snippet: Fixed cells were first incubated with primary antibodies (PAX7 (DSHB), CD29 (BD Biosciences) and CD56 (BD Biosciences) following by secondary antibodies (Alexa350-conjugated goat anti-mouse IgG, Alexa488-conjugated goat anti-rat IgG and Alexa546-conjugated donkey anti-goat IgG, Life Technologies).

    Techniques: Flow Cytometry, FACS, Staining, Immunofluorescence, Cell Culture

    Characterization of bone marrow-derived MSCs. Notes: ( A ) Microscopy images of BMSCs phenotype on day 14 (i), osteogenesis and alkaline phosphate activity staining (ii), and adipogenesis and Oil Red O (iii). Magnification 100×. ( B ) Immunophenotypic analysis of cultured BMSCs with monoclonal antibodies. Flow cytometry analysis results showed that the cells were positive for MSC markers CD29, CD44, CD90, CD105, and negative for markers CD34 and CD45. Scale bar: 200 µm. Abbreviations: BMSCs, bone marrow-derived mesenchymal stem cells; CD, cluster of differentiation; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Journal: International Journal of Nanomedicine

    Article Title: Exosome-mediated delivery of functionally active miRNA-142-3p inhibitor reduces tumorigenicity of breast cancer in vitro and in vivo

    doi: 10.2147/IJN.S182384

    Figure Lengend Snippet: Characterization of bone marrow-derived MSCs. Notes: ( A ) Microscopy images of BMSCs phenotype on day 14 (i), osteogenesis and alkaline phosphate activity staining (ii), and adipogenesis and Oil Red O (iii). Magnification 100×. ( B ) Immunophenotypic analysis of cultured BMSCs with monoclonal antibodies. Flow cytometry analysis results showed that the cells were positive for MSC markers CD29, CD44, CD90, CD105, and negative for markers CD34 and CD45. Scale bar: 200 µm. Abbreviations: BMSCs, bone marrow-derived mesenchymal stem cells; CD, cluster of differentiation; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Article Snippet: CD29, CD44, CD90, CD105, CD34, and CD45 antibodies were obtained from eBioscience (San Diego, CA, USA).

    Techniques: Derivative Assay, Microscopy, Activity Assay, Staining, Cell Culture, Flow Cytometry, Cytometry

    The verification of the BMSCs and the change of IRS-1, TAZ, RUNX2 and OCN mRNA expression during osteogenic differentiation. (A) The BMSCs were isolated from bone marrow, cultured in vitrol and verified by flow cytometry for hematopoietic surface markers CD34 (0.6%) and CD45 (1.6%), and BMSC surface markers CD29 (100%) and CD90 (96%). (B,C) Passage 3 BMSCs were cultured with growth medium or (C) osteogenic medium for 14 days. (D) Alizarin Red staining was performed and (E) the quantification by cetylpyridinium chloride dissolution was presented after inducing osteogenic medium for 14 days. (F) The mRNA expression of IRS-1, TAZ, RUNX2 and CON in BMSCs cultured in osteogenic medium for 3, 7 and 14 days were measured by fluorogenic quantitative PCR. (s.d.±mean; n =3) * P

    Journal: Biology Open

    Article Title: IRS-1 increases TAZ expression and promotes osteogenic differentiation in rat bone marrow mesenchymal stem cells

    doi: 10.1242/bio.036194

    Figure Lengend Snippet: The verification of the BMSCs and the change of IRS-1, TAZ, RUNX2 and OCN mRNA expression during osteogenic differentiation. (A) The BMSCs were isolated from bone marrow, cultured in vitrol and verified by flow cytometry for hematopoietic surface markers CD34 (0.6%) and CD45 (1.6%), and BMSC surface markers CD29 (100%) and CD90 (96%). (B,C) Passage 3 BMSCs were cultured with growth medium or (C) osteogenic medium for 14 days. (D) Alizarin Red staining was performed and (E) the quantification by cetylpyridinium chloride dissolution was presented after inducing osteogenic medium for 14 days. (F) The mRNA expression of IRS-1, TAZ, RUNX2 and CON in BMSCs cultured in osteogenic medium for 3, 7 and 14 days were measured by fluorogenic quantitative PCR. (s.d.±mean; n =3) * P

    Article Snippet: In order to identify the expression levels of classical cells' surface markers, passage 3 BMSCs were stained with anti-CD34, anti-CD45, anti-CD29 and anti-CD90 (all from Thermo Fisher Scientific) in the presence of phycoerythrin (PE) and sorted by fluorescence-activated cell sorting.

    Techniques: Expressing, Isolation, Cell Culture, Flow Cytometry, Cytometry, Staining, Real-time Polymerase Chain Reaction

    Phenotypic analysis of fetal liver MSC. (A). The expression of hepatocyte-specific markers such as ALB, CK8, CK18, CK19, c-Met and Hep Ag in hFLMSC was examined by immunocytochemistry. First row: negative controls, anti-goat and -mouse secondary antibodies. ALB staining was not detected in hFLMSC. Cells expressing CK8 (brown staining) were detected. Magnification 40 X. Second row: expression of CK18 (brown staining) was detected. A few cells expressed c-Met. hFLMSC did not express CK19 and Hep Ag. Magnification 40 X. (B) hFLMSC were characterized with regard to expression of CD44, CD 106, CD29, CD45, CD73, CD 166, CD54 and CD34 using flow cytometry. First row: hFLMSC showed strong expression of CD44 and weak expression of CD29. Expression of CD 106 and CD45 was not observed. Second row: expression of CD73, CD 166 and CD54 was strong on hFLMSC, while expression of CD34 was not detected.

    Journal: Cytotherapy

    Article Title: Fetal liver-derived mesenchymal stromal cells augment engraftment of transplanted hepatocytes

    doi: 10.3109/14653249.2012.663526

    Figure Lengend Snippet: Phenotypic analysis of fetal liver MSC. (A). The expression of hepatocyte-specific markers such as ALB, CK8, CK18, CK19, c-Met and Hep Ag in hFLMSC was examined by immunocytochemistry. First row: negative controls, anti-goat and -mouse secondary antibodies. ALB staining was not detected in hFLMSC. Cells expressing CK8 (brown staining) were detected. Magnification 40 X. Second row: expression of CK18 (brown staining) was detected. A few cells expressed c-Met. hFLMSC did not express CK19 and Hep Ag. Magnification 40 X. (B) hFLMSC were characterized with regard to expression of CD44, CD 106, CD29, CD45, CD73, CD 166, CD54 and CD34 using flow cytometry. First row: hFLMSC showed strong expression of CD44 and weak expression of CD29. Expression of CD 106 and CD45 was not observed. Second row: expression of CD73, CD 166 and CD54 was strong on hFLMSC, while expression of CD34 was not detected.

    Article Snippet: Phenotypic analysis of hFLMSC by flow cytometry Phenotypic characterization of cultured hFLMSC (passages 8, 10 and 11) by flow cytometry was performed using directly conjugated primary antibodies, including CD45-fluorescein isothiocyanate (FITC), CD34-phycoerythrin (PE), CD29-PE, CD54-PE, CD29-PE, CD106-PE, CD166-PE, CD44-PE, CD73-PE and CD29-PE (all from Becton Dickinson, Stockholm, Sweden).

    Techniques: Expressing, Immunocytochemistry, Staining, Flow Cytometry, Cytometry

    Characteristics of MSCs/BM. Cells were stained with the CD45, CD90 and CD29 antibodies and analyzed by flow cytometry. A) BM-MSCs are shown as a dot plot. B) The expression levels of CD45-ve, C) CD90 + ve and D) CD29 + ve of BM-MSCs are presented as a histogram. MSCs/BM, bone marrow-derived mesenchymal stem cells.

    Journal: Stem Cell Research & Therapy

    Article Title: Evaluation of mesenchymal stem cells in treatment of infertility in male rats

    doi: 10.1186/scrt521

    Figure Lengend Snippet: Characteristics of MSCs/BM. Cells were stained with the CD45, CD90 and CD29 antibodies and analyzed by flow cytometry. A) BM-MSCs are shown as a dot plot. B) The expression levels of CD45-ve, C) CD90 + ve and D) CD29 + ve of BM-MSCs are presented as a histogram. MSCs/BM, bone marrow-derived mesenchymal stem cells.

    Article Snippet: The MSCs are positive for CD29 (Sigma, San Diego, CA, USA, SAB 4501582) and negative for CD45 (Sigma, OX-1 84112004) (Figure A-D).

    Techniques: Staining, Flow Cytometry, Cytometry, Expressing, Derivative Assay

    Characterization of Murine Sca-1 + CD29 + CD45 − CD11b − BMSCs

    Journal: Cell stem cell

    Article Title: Inhibition of Sca-1-Positve Skeletal Stem Cell Recruitment by Alendronate Blunts the Anabolic Effects of Parathyroid Hormone on Bone Remodeling

    doi: 10.1016/j.stem.2010.09.012

    Figure Lengend Snippet: Characterization of Murine Sca-1 + CD29 + CD45 − CD11b − BMSCs

    Article Snippet: Cells aliquots were incubated for 20 minutes at 4°C with phycoerythrin (PE)-, fluorescein isothiocyanate (FITC)-, peridinin chlorophyll protein (Per CP)-, and allophycocyanin (APC)-conjugated antibodies against mouse Sca-1, CD29, CD45, and CD11b (Bio-legend).

    Techniques:

    Comparison of MSCs derived from 2D-hESCs and 3D-hESCs. a The morphology of 2D- and 3D-hESC-MSCs at different stages of differentiation. b Flow cytometry analysis revealed specific MSC surface markers (CD44, CD29, and CD105) with negative controls (CD34, CD19, and CD45) in 2D- and 3D-hESC-MSCs. c Immunostaining of differentiated 3D-hESC-MSCs expressing an adipocyte marker (FABP-4), osteocytes maker (osteocalcin), and chondrocytes marker (aggrecan)

    Journal: Cell Death & Disease

    Article Title: A fully defined static suspension culture system for large-scale human embryonic stem cell production

    doi: 10.1038/s41419-018-0863-8

    Figure Lengend Snippet: Comparison of MSCs derived from 2D-hESCs and 3D-hESCs. a The morphology of 2D- and 3D-hESC-MSCs at different stages of differentiation. b Flow cytometry analysis revealed specific MSC surface markers (CD44, CD29, and CD105) with negative controls (CD34, CD19, and CD45) in 2D- and 3D-hESC-MSCs. c Immunostaining of differentiated 3D-hESC-MSCs expressing an adipocyte marker (FABP-4), osteocytes maker (osteocalcin), and chondrocytes marker (aggrecan)

    Article Snippet: For direct labeling, MSCs were stained with FITC anti-CD44 (BD, 555478), PE anti-CD29 (Biolegend, 303004), PE anti-CD105 (Biolegend, 323206), PE anti-CD34 (BD, 555822), FITC anti-CD19 (BD, 555412), FITC anti-CD45 (eBioscience, 11-9459-42), and analyzed using FlowJo software.

    Techniques: Derivative Assay, Flow Cytometry, Cytometry, Immunostaining, Expressing, Marker

    Culture, isolation and identification of human DPSCs. (a, b) Primary cultured DPSCs. (c–f) Odontogenic differentiation of DPSCs was assessed by ALP and Alizarin Red S staining. (g) Flow cytometry was used to detect the surface markers of DPSCs. Cells were incubated with fluorescence-conjugated antibodies against CD29, CD34, CD44, and CD45. Isotype-identical antibodies served as controls. Analysis of surface antigens in DPSCs by flow cytometry indicated that the cells were positive for CD29 and CD44, while CD34 and CD45 were negative (red line).

    Journal: BioMed Research International

    Article Title: Characterization of Odontogenic Differentiation from Human Dental Pulp Stem Cells Using TMT-Based Proteomic Analysis

    doi: 10.1155/2020/3871496

    Figure Lengend Snippet: Culture, isolation and identification of human DPSCs. (a, b) Primary cultured DPSCs. (c–f) Odontogenic differentiation of DPSCs was assessed by ALP and Alizarin Red S staining. (g) Flow cytometry was used to detect the surface markers of DPSCs. Cells were incubated with fluorescence-conjugated antibodies against CD29, CD34, CD44, and CD45. Isotype-identical antibodies served as controls. Analysis of surface antigens in DPSCs by flow cytometry indicated that the cells were positive for CD29 and CD44, while CD34 and CD45 were negative (red line).

    Article Snippet: DPSCs were identified by flow cytometry (Becton Dickinson, Tokyo, Japan). hDPSCs were stained with anti-phycoerythrin (PE), anti-fluorescein isothiocyanate (FITC), anti-CD44-FITC, anti-CD29-PE, anti-CD45-PE, and anti-CD34-PE (BD Pharmingen, Franklin Lakes, NJ) antibodies.

    Techniques: Isolation, Cell Culture, Staining, Flow Cytometry, Incubation, Fluorescence

    Bone mesenchymal stem cells (BMSCs) identification and efficiency of transduction. (A) Flow cytometry analysis for CD29, CD45, CD90 and CD105. (B) The immunofluorescent micrograph of BMSCs infected with hypoxia-inducible factor 1α (Hif-1α)-GFP lentiviral vector, Hif-1α siRNA or their negative controls (NCs). Scale bar = 200 μm. (C) Efficiency of gene transduction was detected by flow cytometry analysis.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Hif-1α Overexpression Improves Transplanted Bone Mesenchymal Stem Cells Survival in Rat MCAO Stroke Model

    doi: 10.3389/fnmol.2017.00080

    Figure Lengend Snippet: Bone mesenchymal stem cells (BMSCs) identification and efficiency of transduction. (A) Flow cytometry analysis for CD29, CD45, CD90 and CD105. (B) The immunofluorescent micrograph of BMSCs infected with hypoxia-inducible factor 1α (Hif-1α)-GFP lentiviral vector, Hif-1α siRNA or their negative controls (NCs). Scale bar = 200 μm. (C) Efficiency of gene transduction was detected by flow cytometry analysis.

    Article Snippet: Cells were incubated for 20 min at 4°C with the following antibodies: anti-CD29, anti-CD90, anti-CD105 and anti-CD45 (Biolegend, CA, USA).

    Techniques: Transduction, Flow Cytometry, Cytometry, Infection, Plasmid Preparation

    Characteristics of HO-1/BMMSCs in vitro and detection of HO-1 expression. a HO-1/BMMSCs were adherent and displayed long spindle-shaped morphology. b HO-1/BMMSCs showed osteogenic differentiation in vitro, as indicated by the calcareous deposits stained black with von Kossa staining. c Adipogenic differentiation of HO-1/BMMSCs, as indicated by oil red O-stained fat cells. d – f Surface biomarker identification. Results showed that 100.0, 99.8, and 99.4% of the cells were positive for CD29, CD90, and RT1A, respectively, and 100.0, 99.8, and 99.4% of the cells were negative for CD34, CD45, and RT1B, respectively. The molecular biological characteristics of HO-1/BMMSCs were not changed. g HO-1 expression in BMMSCs was identified by red fluorescence, and its intensity was weak. h HO-1 expression in HO-1/BMMSCs. The red fluorescence intensity was significantly higher in HO-1/BMMSCs than that in BMMSCs. i Western blotting and qRT-PCR results confirmed that HO-1 expression in HO-1/BMMSCs was significantly higher than that in BMMSCs

    Journal: Stem Cell Research & Therapy

    Article Title: Heme oxygenase-1-modified bone marrow mesenchymal stem cells combined with normothermic machine perfusion to protect donation after circulatory death liver grafts

    doi: 10.1186/s13287-020-01736-1

    Figure Lengend Snippet: Characteristics of HO-1/BMMSCs in vitro and detection of HO-1 expression. a HO-1/BMMSCs were adherent and displayed long spindle-shaped morphology. b HO-1/BMMSCs showed osteogenic differentiation in vitro, as indicated by the calcareous deposits stained black with von Kossa staining. c Adipogenic differentiation of HO-1/BMMSCs, as indicated by oil red O-stained fat cells. d – f Surface biomarker identification. Results showed that 100.0, 99.8, and 99.4% of the cells were positive for CD29, CD90, and RT1A, respectively, and 100.0, 99.8, and 99.4% of the cells were negative for CD34, CD45, and RT1B, respectively. The molecular biological characteristics of HO-1/BMMSCs were not changed. g HO-1 expression in BMMSCs was identified by red fluorescence, and its intensity was weak. h HO-1 expression in HO-1/BMMSCs. The red fluorescence intensity was significantly higher in HO-1/BMMSCs than that in BMMSCs. i Western blotting and qRT-PCR results confirmed that HO-1 expression in HO-1/BMMSCs was significantly higher than that in BMMSCs

    Article Snippet: Antibodies against CD29, CD34, CD45, CD90, RT1A, and RT1B (BioLegend, San Diego, CA, USA) were used for phenotype identification by flow cytometry.

    Techniques: In Vitro, Expressing, Staining, Biomarker Assay, Fluorescence, Western Blot, Quantitative RT-PCR

    Characterization of adipose derived stem cells (ADSC). a ADSC were analyzed for cell surface markers in passage 6 (P6) and passage 11 (P11). Over 90% of cells were positive for CD90 and CD29 and negative for CD45 and CD11b/c in both passages. Paired t-test between P6 and P11 showed no differences in expression of surface markers. ADSC in passage 4 were differentiated into adipocytes ( b ), osteocytes ( c ), and chondrocytes ( d ). Lipid vacuoles were visualized with oil red O staining ( b ), calcium deposits were stained with Alizarin Red S ( c ), and proteoglycans of chondrogenic pellets were detected by Alcian blue staining ( d ). Inserts represent ADSC, cultured in proliferation medium as negative controls

    Journal: BMC Biotechnology

    Article Title: Myogenic differentiation of primary myoblasts and mesenchymal stromal cells under serum-free conditions on PCL-collagen I-nanoscaffolds

    doi: 10.1186/s12896-018-0482-6

    Figure Lengend Snippet: Characterization of adipose derived stem cells (ADSC). a ADSC were analyzed for cell surface markers in passage 6 (P6) and passage 11 (P11). Over 90% of cells were positive for CD90 and CD29 and negative for CD45 and CD11b/c in both passages. Paired t-test between P6 and P11 showed no differences in expression of surface markers. ADSC in passage 4 were differentiated into adipocytes ( b ), osteocytes ( c ), and chondrocytes ( d ). Lipid vacuoles were visualized with oil red O staining ( b ), calcium deposits were stained with Alizarin Red S ( c ), and proteoglycans of chondrogenic pellets were detected by Alcian blue staining ( d ). Inserts represent ADSC, cultured in proliferation medium as negative controls

    Article Snippet: Cells were incubated with the following fluorochrome-conjugated antibodies: CD90, CD29, CD59, CD45, CD11b/c (Miltenyi Biotec GmbH).

    Techniques: Derivative Assay, Expressing, Staining, Cell Culture

    Surface marker expression of in-vitro aging MSCs. MSCs were cultivated with either DMEM-based ( a ) or αMEM-based ( b ) expansion media and were analyzed by flow cytometry for the expression of specific MSC positive (Stro-1, CD29, CD44, CD73, CD90, CD105, CD106, CD 146) and negative (CD34, CD45) surface antigens. n = 4–6; * p

    Journal: Stem Cell Research & Therapy

    Article Title: Changes in phenotype and differentiation potential of human mesenchymal stem cells aging in vitro

    doi: 10.1186/s13287-018-0876-3

    Figure Lengend Snippet: Surface marker expression of in-vitro aging MSCs. MSCs were cultivated with either DMEM-based ( a ) or αMEM-based ( b ) expansion media and were analyzed by flow cytometry for the expression of specific MSC positive (Stro-1, CD29, CD44, CD73, CD90, CD105, CD106, CD 146) and negative (CD34, CD45) surface antigens. n = 4–6; * p

    Article Snippet: Specifically, antibodies against Stro-1 (ab190282) and CD73 (ab106677) were purchased from Abcam (Cambridge, MA, USA); CD29 (MCA1949A647), CD34 (MCA547PE), CD44 (MCA89PE), and CD106 (MCA907F) were from Bio-Rad (Kidlington, Oxford, UK); and CD45, CD90, CD105, and CD146 (FM002) were from R & D Systems (Minneapolis, MN, USA).

    Techniques: Marker, Expressing, In Vitro, Flow Cytometry, Cytometry