Journal: Cancer Medicine
Article Title: Downregulated hsa_circ_0077837 and hsa_circ_0004826, facilitate bladder cancer progression and predict poor prognosis for bladder cancer patients, et al. Downregulated hsa_circ_0077837 and hsa_circ_0004826, facilitate bladder cancer progression and predict poor prognosis for bladder cancer patients
Figure Lengend Snippet: Both circ_0077837 and circ_0004826 exerted a tumor suppressive effect in BC cells. A, Transgene expression in EJ and 253J‐BV bladder cancer cell lines transduced with lentiviral vectors. Upper panels show phase contrast photomicrograph and lower panels show GFP fluorescence of the same field. B, Overexpression of circ_0077837 and circ_0004826 was confirmed via qRT‐PCR in BC cell lines EJ and 253J‐BV. C, Cell colony formation numbers were counted after transfection with OV‐NC, OV‐circ_7837, or OV‐circ_4826. D, CCK‐8 assays were utilized to detect cell proliferation ability in EJ and 253J‐BV cells transfected with OV‐NC, OV‐circ_7837, or OV‐circ_4826. E and F, The migration potential of EJ and 253J‐BV cells transfected with the OV‐circ_7837 or OV‐circ_4826 than that treated with OV‐NC was damaged by the wound healing assay at 36 h after scratch and transwell migration assay (without Matrigel) at 36 h after incubation. G, Overexpression of OV‐circ_7837 or OV‐circ_4826 all impaired the invasive capacity of EJ and 253J‐BV cells as detected by transwell Matrigel invasion assay. Data are presented as means ± SD; n = 3. SD: standard deviation. OV‐: Overexpression; NC, empty vector; Circ‐7837, hsa_circ_0077837; Circ‐4826, hsa_circ_0004826; CCK‐8: cell counting kit‐8; OD: optical density. * P
Article Snippet: Subsequently, each well was supplemented with 10 μL CCK‐8 solution (Boster Bio) and incubated for 3.5 hours in dark.
Techniques: Expressing, Transduction, Fluorescence, Over Expression, Quantitative RT-PCR, Transfection, CCK-8 Assay, Migration, Wound Healing Assay, Transwell Migration Assay, Incubation, Invasion Assay, Standard Deviation, Plasmid Preparation, Cell Counting