Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: Long non-coding RNA small nucleolar RNA host gene 12 (SNHG12) promotes tumorigenesis and metastasis by targeting miR-199a/b-5p in hepatocellular carcinoma
Figure Lengend Snippet: The functional analysis of SNHG12 in HCC cells. a The SNHG12 expression level was determined by qPCR when SK-Hep1 and HCCLM9 cells were transfected with SNHG12-siRNA. b CCK-8 assay was applied to detect the proliferation of SK-Hep1 cells. c CCK-8 assay was applied to detect the proliferation of HCCLM9 cells. d and e Flow cytometry assays were performed to analyze the apoptosis of SK-Hep1 and HCCLM9 cells after treatment with SNHG12-siRNA and stained with apoptosis markers (FITC-Annexin V and PI). f and g The ability of cancer cell invasion was measured by using transwell assay when SNHG12 was downregulated in SK-Hep1 and HCCLM9 cells [original magnification, ×250 and × 100]. h and i The ability of cancer cell migration was measured by using wound-healing assay when SNHG12 was downregulated in SK-Hep1 and HCCLM9 cells [original magnification, ×100]. Data represented the mean ± SD from three independent experiments. * P
Article Snippet: Cell proliferation assay A total of 3 × 104 cells were seeded in each well of a 96-well plate, and incubated for 24 h. Ten Microliter WST-8 from the CCK-8 Kit (Boster, Wuhan, China) was then added to each well.
Techniques: Functional Assay, Expressing, Real-time Polymerase Chain Reaction, Transfection, CCK-8 Assay, Flow Cytometry, Cytometry, Staining, Transwell Assay, Migration, Wound Healing Assay