cccdna pcr quantification Search Results


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  • 99
    Thermo Fisher real time quantitative pcr
    Real Time Quantitative Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Roche cobas amplicor polymerase chain reaction pcr assay assay
    Cobas Amplicor Polymerase Chain Reaction Pcr Assay Assay, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche polymerase chain reaction qpcr
    Biomarker analysis in <t>HBV-infected</t> HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) <t>RT-qPCR</t> analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.
    Polymerase Chain Reaction Qpcr, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    polymerase chain reaction qpcr - by Bioz Stars, 2020-08
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    92
    Shanghai Fosun Pharmaceutical real time polymerase chain reaction pcr
    Biomarker analysis in <t>HBV-infected</t> HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) <t>RT-qPCR</t> analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.
    Real Time Polymerase Chain Reaction Pcr, supplied by Shanghai Fosun Pharmaceutical, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Roche quantitative pcr system
    Biomarker analysis in <t>HBV-infected</t> HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) <t>RT-qPCR</t> analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.
    Quantitative Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 2875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Qiagen quantifast probe pcr kit
    Biomarker analysis in <t>HBV-infected</t> HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) <t>RT-qPCR</t> analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.
    Quantifast Probe Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche pcr mixture
    Biomarker analysis in <t>HBV-infected</t> HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) <t>RT-qPCR</t> analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.
    Pcr Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 2412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher pcr
    Biomarker analysis in <t>HBV-infected</t> HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) <t>RT-qPCR</t> analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.
    Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 111176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Shanghai Fosun Pharmaceutical time quantitative pcr kit
    Biomarker analysis in <t>HBV-infected</t> HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) <t>RT-qPCR</t> analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.
    Time Quantitative Pcr Kit, supplied by Shanghai Fosun Pharmaceutical, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen quantinova sybr green pcr kit
    Biomarker analysis in <t>HBV-infected</t> HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) <t>RT-qPCR</t> analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.
    Quantinova Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1668 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Sangon Biotech transcription quantitative pcr
    The identification of <t>DHBV-positive</t> serum by <t>PCR.</t> The size of the amplified PCR product was 292 bp. Lane 1, DHBV positive serum; and lane 2, DNA marker. DHBV, duck hepatitis B virus; PCR, polymerase chain reaction.
    Transcription Quantitative Pcr, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    PerkinElmer pcr buffer ii
    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
    Pcr Buffer Ii, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 914 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcr reaction mix
    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
    Pcr Reaction Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Roche time pcr qpcr
    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
    Time Pcr Qpcr, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr 2 1 topo
    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
    Pcr 2 1 Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Qiagen artus hbv tm pcr kit
    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
    Artus Hbv Tm Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 89/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher stepone real time pcr system
    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
    Stepone Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19014 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green pcr master mix
    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
    Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 101787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7500 rt pcr system
    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
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    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
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    Sensitivity and specificity of <t>LP-PCR</t> and comparison with a nested Alu PCR protocol. (A to C) Viral <t>DNA</t> accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).
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    Image Search Results


    Biomarker analysis in HBV-infected HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) RT-qPCR analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.

    Journal: Gastroenterology

    Article Title: Viral Load Affects the Immune Response to HBV in Mice With Humanized Immune System and Liver

    doi: 10.1053/j.gastro.2017.08.034

    Figure Lengend Snippet: Biomarker analysis in HBV-infected HIS-HUHEP mice. ( A ) Plasma from HIS-HUHEP control (n=11) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=10) at endpoint were analyzed using human cytokine multiplex assay. No cross-reactivity with mouse cytokines was detected. Plasma levels of IL-1β, IL-1Ra, IL-2, IL-2R, IL-4, IL-12, IL-13, IL-15, IFN-γ, GM-CSF, and RANTES were similar between control and HBV-infected mice; while IL-5, IL-17, and Eotaxin were not detected (data not shown). ( B ) Correlation of PD-1 + memory CD4 + CD45RO + T cells with either IFN-γ, or IP-10/CXCL10, or IL-10 plasma cytokines quantified in ( A ). Each dot represents a mouse: grey or black inoculated with, respectively, HBV 10e7 or HBV 10e9. ( C ) RT-qPCR analysis of liver samples from HIS-HUHEP control (n=14) and HBV-infected mice (inoculum 10e7, n=5; or 10e9, n=9). Fold changes in gene expression of HBV-infected compared with control mice are shown. Data was normalized to the internal control human GAPDH (hGAPDH) to account for differences in humanization levels on triplicate samples. Dotted line indicates fold change of 1. Histograms show the mean and SEM. Data from 14–20 wpi. Statistical significance: Mann Whitney U tests.

    Article Snippet: Plasmatic HBV DNA was quantified by quantitative polymerase chain reaction (qPCR) with a LightCycler system (Roche, Basel, Switzerland) as described by Brezillon et al. Intrahepatic HBV DNA was quantified by SYBR Green qPCR on an ABI PRISM 7900HT system (Applied Biosystems, Foster City, CA).

    Techniques: Biomarker Assay, Infection, Mouse Assay, Multiplex Assay, Quantitative RT-PCR, Expressing, MANN-WHITNEY

    The identification of DHBV-positive serum by PCR. The size of the amplified PCR product was 292 bp. Lane 1, DHBV positive serum; and lane 2, DNA marker. DHBV, duck hepatitis B virus; PCR, polymerase chain reaction.

    Journal: Molecular Medicine Reports

    Article Title: Efficient inhibition of duck hepatitis B virus DNA by the CRISPR/Cas9 system

    doi: 10.3892/mmr.2017.7518

    Figure Lengend Snippet: The identification of DHBV-positive serum by PCR. The size of the amplified PCR product was 292 bp. Lane 1, DHBV positive serum; and lane 2, DNA marker. DHBV, duck hepatitis B virus; PCR, polymerase chain reaction.

    Article Snippet: Quantification of DHBV DNA in the culture medium and PDHs DHBV total DNA and cccDNA were then quantified by reverse transcription-quantitative PCR with specific primers and TaqMan MGB probes (Sangon Biotech Co., Ltd, Shanghai, China).

    Techniques: Polymerase Chain Reaction, Amplification, Marker

    Sensitivity and specificity of LP-PCR and comparison with a nested Alu PCR protocol. (A to C) Viral DNA accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).

    Journal: Journal of Virology

    Article Title: Kinetics of Human Immunodeficiency Virus Type 1 (HIV) DNA Integration in Acutely Infected Cells as Determined Using a Novel Assay for Detection of Integrated HIV DNA

    doi: 10.1128/JVI.75.22.11253-11260.2001

    Figure Lengend Snippet: Sensitivity and specificity of LP-PCR and comparison with a nested Alu PCR protocol. (A to C) Viral DNA accumulation following cell-free infection in the presence or absence of inhibitors. HuT-78 T cells were infected using the centrifugal enhancement protocol at 0.5 TCID 50 per cell and cellular DNA prepared from infected cells harvested at 26 h p.i. 3TC and L-731,988 were used as specific inhibitors of reverse transcription and integration, respectively. (A) Sensitivity of LP-PCR (as measured by amplification of the HA8 integrated HIV DNA standard) and integrated HIV DNA accumulation following infection as measured by LP-PCR performed on 100 cell equivalents of Hirt pellet (chromosomal) DNA preparations. (B) Total reverse-transcribed DNA as measured by GAG-PCR performed on combined Hirt supernatant (extrachromosomal) and Hirt pellet (chromosomal) DNA samples. (C) Graphical representation of the accumulation of integrated HIV DNA. Data were obtained by PhosphorImager analysis of the bands in panel A. (D) Comparison of PCR detection of integrated HIV DNA by LP-PCR and Alu PCR. Chromosomal DNA was isolated from ACH-2 or 8E5 cells and shown to contain equivalent amounts of total HIV DNA by GAG-PCR (314-bp band). Sizes of expected bands for LP-PCR (measuring integrated HIV DNA) are given on the left (104-bp fragment), while the expected size of the product obtained following Alu PCR (also measuring integrated HIV DNA) is indicated on the right (351-bp fragment).

    Article Snippet: Quantification of 2-LTR circular DNA following infection was achieved by performing PCR on 500 cell equivalents of total DNA in 1× PCR Buffer II (Perkin-Elmer), 1.5 mM MgCl2 , and 0.2 mM dNTPs using 25 pmol each of primers R7 and U3PNV and 1.5 U of AmpliTaq Gold DNA polymerase.

    Techniques: Polymerase Chain Reaction, Infection, Amplification, Isolation

    Accumulation kinetics of total, integrated, and 2-LTR viral DNA forms following high-multiplicity infection of HuT-78 T cells. Infections were performed using 1 TCID 50 of HIV HXB2 per cell with centrifugal enhancement. All PCRs were confirmed to amplify DNA in a linear fashion by quantification of standards (A to D) or dilution sets (E) (dilutions not shown). DNA markers (pUC19/ Hpa II) are indicated (M). (A) Total HIV DNA forms as measured by GAG-PCR using 500 cell equivalents of total DNA (combined Hirt supernatant and Hirt pellet). (B.i) Integrated HIV DNA levels as measured by LP-PCR on 100 cell equivalents of chromosomal DNA (Hirt pellet). (B.ii) Integrated HIV DNA levels as measured by the modified nested Alu PCR method performed on 1,000 cell equivalents of chromosomal DNA. (C.i) 2-LTR HIV DNA levels as measured by 2-LTR PCR on 500 cell equivalents of total DNA. (C.ii) Reanalysis of later time points for the 2-LTR DNA forms using 1,000 cell equivalents of total DNA. (D) β-Globin levels assayed by PCR on 50 cell equivalents of chromosomal DNA. Standards represent amplification of various amounts (based on cell counts) of HA8 chromosomal DNA. (E) Mitochondrial DNA levels assayed by PCR on 50 cell equivalents of Hirt supernatant (extrachromosomal) fraction.

    Journal: Journal of Virology

    Article Title: Kinetics of Human Immunodeficiency Virus Type 1 (HIV) DNA Integration in Acutely Infected Cells as Determined Using a Novel Assay for Detection of Integrated HIV DNA

    doi: 10.1128/JVI.75.22.11253-11260.2001

    Figure Lengend Snippet: Accumulation kinetics of total, integrated, and 2-LTR viral DNA forms following high-multiplicity infection of HuT-78 T cells. Infections were performed using 1 TCID 50 of HIV HXB2 per cell with centrifugal enhancement. All PCRs were confirmed to amplify DNA in a linear fashion by quantification of standards (A to D) or dilution sets (E) (dilutions not shown). DNA markers (pUC19/ Hpa II) are indicated (M). (A) Total HIV DNA forms as measured by GAG-PCR using 500 cell equivalents of total DNA (combined Hirt supernatant and Hirt pellet). (B.i) Integrated HIV DNA levels as measured by LP-PCR on 100 cell equivalents of chromosomal DNA (Hirt pellet). (B.ii) Integrated HIV DNA levels as measured by the modified nested Alu PCR method performed on 1,000 cell equivalents of chromosomal DNA. (C.i) 2-LTR HIV DNA levels as measured by 2-LTR PCR on 500 cell equivalents of total DNA. (C.ii) Reanalysis of later time points for the 2-LTR DNA forms using 1,000 cell equivalents of total DNA. (D) β-Globin levels assayed by PCR on 50 cell equivalents of chromosomal DNA. Standards represent amplification of various amounts (based on cell counts) of HA8 chromosomal DNA. (E) Mitochondrial DNA levels assayed by PCR on 50 cell equivalents of Hirt supernatant (extrachromosomal) fraction.

    Article Snippet: Quantification of 2-LTR circular DNA following infection was achieved by performing PCR on 500 cell equivalents of total DNA in 1× PCR Buffer II (Perkin-Elmer), 1.5 mM MgCl2 , and 0.2 mM dNTPs using 25 pmol each of primers R7 and U3PNV and 1.5 U of AmpliTaq Gold DNA polymerase.

    Techniques: Infection, Polymerase Chain Reaction, Modification, Amplification