cccdna detection Search Results


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  • 99
    New England Biolabs circular single stranded m13mp18 viral dna
    Circular Single Stranded M13mp18 Viral Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mung bean digestion buffer
    Mung Bean Digestion Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Integrated DNA Technologies cccdna
    Cccdna, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare detection kit
    Detection Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Olink Bioscience duolink detection kit 563
    Duolink Detection Kit 563, supplied by Olink Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    GE Healthcare cdp star detection reagent
    Cdp Star Detection Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 684 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche detection starter kit
    Detection Starter Kit, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 1253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer abi prism 7300 sequence detection system
    Abi Prism 7300 Sequence Detection System, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa rt pcr detection
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Rt Pcr Detection, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher abi prism 7500 sequence detection system
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Abi Prism 7500 Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 17066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad cfx96 real time pcr detection system
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Cfx96 Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 27018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher ab7900 ht sequence detection system
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Ab7900 Ht Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Roche quantitative real time pcr detection
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Quantitative Real Time Pcr Detection, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 6939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Roche pcr digoxigenin antidigoxigenin detection dig labelled probe
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Pcr Digoxigenin Antidigoxigenin Detection Dig Labelled Probe, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad icycler iq real time pcr detection system
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Icycler Iq Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 9356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad cfx96 touch real time pcr detection system
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Cfx96 Touch Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore circular puc19 dna
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Circular Puc19 Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche lightcycler instrument
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Lightcycler Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 6846 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen qiaamp dna mini kit
    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. <t>mRNAs</t> for SHP as well as NTCP and GAPDH were detected by <t>RT-PCR.</t> B, ChIP
    Qiaamp Dna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 48891 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare hbv specific dna probe
    Quantification of <t>HBV</t> <t>DNA</t> in the supernatants of compound-treated HepAD38 cells by real-time PCR at different concentrations of BAY 41-4109 (diamonds), GLS4 (squares), and lamivudine (triangles). The graph shows the means of three independent experiments. Error bars indicate standard deviation based upon the mean value at each data point.
    Hbv Specific Dna Probe, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Roche hbv dna
    Detection of replicative intermediates in primary Tupaia hepatocytes infected with increasing amounts of purified virus. (A) schematic diagram of the early events in <t>HBV</t> infection of hepatocytes. Formation of cccDNA from the incoming HBV rcDNA form with its nick-and-gap structure is essential for RNA transcription and all further steps in establishing an HBV infection. (B) Agarose gel electrophoresis of PCR products specific for HBV double-stranded <t>DNA</t> without nick and gap. A specific amplificate at 735 bp (arrow) could be synthesized from cloned dimeric HBV DNA but not from purified virion rcDNA, with its nick-and-gap structure, from human plasma. HBV cccDNA was detected in nuclear Hirt extracts of HBV-infected primary Tupaia hepatocytes 12 days p.i. or in the positive control cell line HepG2.2.15, but not from the parental HepG2 cell line. Lane M, marker; lane ⊘, negative control. (C) HBV mRNA quantification of cytoplasmic extracts from primary Tupaia hepatocytes 12 days after infection with increasing amounts of purified HBV (real-time RT-PCR of the X-gene region). The detection limit was 10 4 copies per culture. ge, genomic equivalent.
    Hbv Dna, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 2220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BioLegend bm mscs
    Morphological and flow cytometry analysis of <t>BM-MSCs</t> derived from Brown Norway rats. Microscope images of (A) <t>HepG2.2.15</t> cells and (B) BM-MSCs at passage 3 (magnification, ×100). Flow cytometry analysis of the expression of (C) CD29 and CD34, (D) CD45 and CD90, and (E) RT1A and RT1B. BM-MSCs, bone marrow-derived mesenchymal stem cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    Bm Mscs, supplied by BioLegend, used in various techniques. Bioz Stars score: 92/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs t5 exonuclease
    Morphological and flow cytometry analysis of <t>BM-MSCs</t> derived from Brown Norway rats. Microscope images of (A) <t>HepG2.2.15</t> cells and (B) BM-MSCs at passage 3 (magnification, ×100). Flow cytometry analysis of the expression of (C) CD29 and CD34, (D) CD45 and CD90, and (E) RT1A and RT1B. BM-MSCs, bone marrow-derived mesenchymal stem cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    T5 Exonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 588 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    TaKaRa calf thymus topoisomerase i
    Morphological and flow cytometry analysis of <t>BM-MSCs</t> derived from Brown Norway rats. Microscope images of (A) <t>HepG2.2.15</t> cells and (B) BM-MSCs at passage 3 (magnification, ×100). Flow cytometry analysis of the expression of (C) CD29 and CD34, (D) CD45 and CD90, and (E) RT1A and RT1B. BM-MSCs, bone marrow-derived mesenchymal stem cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    Calf Thymus Topoisomerase I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. mRNAs for SHP as well as NTCP and GAPDH were detected by RT-PCR. B, ChIP

    Journal: The Journal of Biological Chemistry

    Article Title: Dysregulation of Retinoic Acid Receptor Diminishes Hepatocyte Permissiveness to Hepatitis B Virus Infection through Modulation of Sodium Taurocholate Cotransporting Polypeptide (NTCP) Expression *

    doi: 10.1074/jbc.M114.602540

    Figure Lengend Snippet: RAR directly regulated the activity of hNTCP promoter. A, HepaRG cells were treated with or without ATRA, Ro41-5253, or a positive control GW4064, which is an FXR agonist, for 24 h. mRNAs for SHP as well as NTCP and GAPDH were detected by RT-PCR. B, ChIP

    Article Snippet: RT-PCR detection of mRNAs for NTCP, ASBT, SHP , and GAPDH was performed with one-step RNA PCR kit (TaKaRa) following the manufacturer's protocol with primer set 5′-AGGGAGGAGGTGGCAATCAAGAGTGG-3′ and 5′-CCGGCTGAAGAACATTGAGGCACTGG-3′ for NTCP , 5′-GTTGGCCTTGGTGATGTTCT-3′ and 5′-CGACCCAATAGGCCAAGATA-3′ for ASBT , 5′-CAGCTATGTGCACCTCATCG-3′ and 5′-CCAGAAGGACTCCAGACAGC-3′ for SHP , and 5′-CCATGGAGAAGGCTGGGG-3′ and 5′-CAAAGTTGTCATGGATGACC-3′ for GAPDH , respectively.

    Techniques: Activity Assay, Positive Control, Reverse Transcription Polymerase Chain Reaction, Chromatin Immunoprecipitation

    Quantification of HBV DNA in the supernatants of compound-treated HepAD38 cells by real-time PCR at different concentrations of BAY 41-4109 (diamonds), GLS4 (squares), and lamivudine (triangles). The graph shows the means of three independent experiments. Error bars indicate standard deviation based upon the mean value at each data point.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Preclinical Characterization of GLS4, an Inhibitor of Hepatitis B Virus Core Particle Assembly

    doi: 10.1128/AAC.01091-13

    Figure Lengend Snippet: Quantification of HBV DNA in the supernatants of compound-treated HepAD38 cells by real-time PCR at different concentrations of BAY 41-4109 (diamonds), GLS4 (squares), and lamivudine (triangles). The graph shows the means of three independent experiments. Error bars indicate standard deviation based upon the mean value at each data point.

    Article Snippet: HBV covalently closed circular DNA (cccDNA), extracted from isolated nuclei of cells replicating virus, was detected by using a 32 P-labeled HBV-specific DNA probe (Rediprime II; GE Healthcare, Piscataway, NJ).

    Techniques: Real-time Polymerase Chain Reaction, Standard Deviation

    Detection of replicative intermediates in primary Tupaia hepatocytes infected with increasing amounts of purified virus. (A) schematic diagram of the early events in HBV infection of hepatocytes. Formation of cccDNA from the incoming HBV rcDNA form with its nick-and-gap structure is essential for RNA transcription and all further steps in establishing an HBV infection. (B) Agarose gel electrophoresis of PCR products specific for HBV double-stranded DNA without nick and gap. A specific amplificate at 735 bp (arrow) could be synthesized from cloned dimeric HBV DNA but not from purified virion rcDNA, with its nick-and-gap structure, from human plasma. HBV cccDNA was detected in nuclear Hirt extracts of HBV-infected primary Tupaia hepatocytes 12 days p.i. or in the positive control cell line HepG2.2.15, but not from the parental HepG2 cell line. Lane M, marker; lane ⊘, negative control. (C) HBV mRNA quantification of cytoplasmic extracts from primary Tupaia hepatocytes 12 days after infection with increasing amounts of purified HBV (real-time RT-PCR of the X-gene region). The detection limit was 10 4 copies per culture. ge, genomic equivalent.

    Journal: Journal of Virology

    Article Title: Pre-S1 Antigen-Dependent Infection of Tupaia Hepatocyte Cultures with Human Hepatitis B Virus

    doi: 10.1128/JVI.77.17.9511-9521.2003

    Figure Lengend Snippet: Detection of replicative intermediates in primary Tupaia hepatocytes infected with increasing amounts of purified virus. (A) schematic diagram of the early events in HBV infection of hepatocytes. Formation of cccDNA from the incoming HBV rcDNA form with its nick-and-gap structure is essential for RNA transcription and all further steps in establishing an HBV infection. (B) Agarose gel electrophoresis of PCR products specific for HBV double-stranded DNA without nick and gap. A specific amplificate at 735 bp (arrow) could be synthesized from cloned dimeric HBV DNA but not from purified virion rcDNA, with its nick-and-gap structure, from human plasma. HBV cccDNA was detected in nuclear Hirt extracts of HBV-infected primary Tupaia hepatocytes 12 days p.i. or in the positive control cell line HepG2.2.15, but not from the parental HepG2 cell line. Lane M, marker; lane ⊘, negative control. (C) HBV mRNA quantification of cytoplasmic extracts from primary Tupaia hepatocytes 12 days after infection with increasing amounts of purified HBV (real-time RT-PCR of the X-gene region). The detection limit was 10 4 copies per culture. ge, genomic equivalent.

    Article Snippet: Eluted HBV DNA was further purified using a DNA extraction kit (Highpure; Roche).

    Techniques: Infection, Purification, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Synthesized, Clone Assay, Positive Control, Marker, Negative Control, Quantitative RT-PCR

    Morphological and flow cytometry analysis of BM-MSCs derived from Brown Norway rats. Microscope images of (A) HepG2.2.15 cells and (B) BM-MSCs at passage 3 (magnification, ×100). Flow cytometry analysis of the expression of (C) CD29 and CD34, (D) CD45 and CD90, and (E) RT1A and RT1B. BM-MSCs, bone marrow-derived mesenchymal stem cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Journal: Molecular Medicine Reports

    Article Title: Biological effects of bone marrow mesenchymal stem cells on hepatitis B virus in vitro

    doi: 10.3892/mmr.2017.6330

    Figure Lengend Snippet: Morphological and flow cytometry analysis of BM-MSCs derived from Brown Norway rats. Microscope images of (A) HepG2.2.15 cells and (B) BM-MSCs at passage 3 (magnification, ×100). Flow cytometry analysis of the expression of (C) CD29 and CD34, (D) CD45 and CD90, and (E) RT1A and RT1B. BM-MSCs, bone marrow-derived mesenchymal stem cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Article Snippet: Detection of intracellular HBV cccDNA in BM-MSCs No intracellular HBV cccDNA was detected in the BM-MSCs in any of the groups (data not shown), which suggested that BM-MSCs co-cultured with HepG2.2.15 cells were not be infected by HBV.

    Techniques: Flow Cytometry, Cytometry, Derivative Assay, Microscopy, Expressing