cathepsin l inhibitor ii Millipore Search Results


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  • 99
    Millipore cathepsin l inhibitor
    Cathepsin activity and cystatin C amount in primary and metastatic human melanoma cells . ( A ) Quantitative cytofluorimetric analysis of plasma membrane cathepsin B, D and L in four different primary (white columns) and metastatic (black columns) melanoma cell lines. Numbers represent the mean values among four different primary or metastatic melanoma cell lines analyzed. ( B ) Fluorimetric assays for cathepsin B (black columns), cathepsin D (grey columns) and <t>cathepsin</t> L (white columns) activity in the cell medium of two representative primary and metastatic melanoma cell lines. Results are reported as fluorescence units. ( C ) Concentration of cystatin C in the growth medium of two representative primary and metastatic melanoma cell lines obtained by ELISA test. Results are reported as ng/ml. (*) p
    Cathepsin L Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cathepsin l inhibitor/product/Millipore
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    cathepsin l inhibitor - by Bioz Stars, 2020-01
    99/100 stars
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    83
    Millipore catl inhibitor li z phe tyr t bu diazomethylketone
    Cathepsin activity and cystatin C amount in primary and metastatic human melanoma cells . ( A ) Quantitative cytofluorimetric analysis of plasma membrane cathepsin B, D and L in four different primary (white columns) and metastatic (black columns) melanoma cell lines. Numbers represent the mean values among four different primary or metastatic melanoma cell lines analyzed. ( B ) Fluorimetric assays for cathepsin B (black columns), cathepsin D (grey columns) and <t>cathepsin</t> L (white columns) activity in the cell medium of two representative primary and metastatic melanoma cell lines. Results are reported as fluorescence units. ( C ) Concentration of cystatin C in the growth medium of two representative primary and metastatic melanoma cell lines obtained by ELISA test. Results are reported as ng/ml. (*) p
    Catl Inhibitor Li Z Phe Tyr T Bu Diazomethylketone, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/catl inhibitor li z phe tyr t bu diazomethylketone/product/Millipore
    Average 83 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    catl inhibitor li z phe tyr t bu diazomethylketone - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

    Image Search Results


    Cathepsin activity and cystatin C amount in primary and metastatic human melanoma cells . ( A ) Quantitative cytofluorimetric analysis of plasma membrane cathepsin B, D and L in four different primary (white columns) and metastatic (black columns) melanoma cell lines. Numbers represent the mean values among four different primary or metastatic melanoma cell lines analyzed. ( B ) Fluorimetric assays for cathepsin B (black columns), cathepsin D (grey columns) and cathepsin L (white columns) activity in the cell medium of two representative primary and metastatic melanoma cell lines. Results are reported as fluorescence units. ( C ) Concentration of cystatin C in the growth medium of two representative primary and metastatic melanoma cell lines obtained by ELISA test. Results are reported as ng/ml. (*) p

    Journal: Molecular Cancer

    Article Title: Cathepsin B inhibition interferes with metastatic potential of human melanoma: an in vitro and in vivo study

    doi: 10.1186/1476-4598-9-207

    Figure Lengend Snippet: Cathepsin activity and cystatin C amount in primary and metastatic human melanoma cells . ( A ) Quantitative cytofluorimetric analysis of plasma membrane cathepsin B, D and L in four different primary (white columns) and metastatic (black columns) melanoma cell lines. Numbers represent the mean values among four different primary or metastatic melanoma cell lines analyzed. ( B ) Fluorimetric assays for cathepsin B (black columns), cathepsin D (grey columns) and cathepsin L (white columns) activity in the cell medium of two representative primary and metastatic melanoma cell lines. Results are reported as fluorescence units. ( C ) Concentration of cystatin C in the growth medium of two representative primary and metastatic melanoma cell lines obtained by ELISA test. Results are reported as ng/ml. (*) p

    Article Snippet: For cell migration and invasion assays, cells were seeded in the presence of the cathepsin D inhibitor (Pepstatin A, 100 μM, Calbiochem), the cathepsin L inhibitor (cathepsin L inhibitor II, Z-FY-CHO, 10 μM, Calbiochem) as well as two cathepsin B inhibitors: CA-074 (cell unpermeant) and CA-074Me (cell permeant) (both given at a concentration of 10 μM, Calbiochem, Nottingham, UK).

    Techniques: Activity Assay, Fluorescence, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Cathepsins and in vitro invasiveness: effect of chemical and biological inhibitors . ( A ) Invasion test on primary (white columns) and metastatic (black columns) melanoma cell lines. ( B ) Invasion test on two representative cell lines from primary (white columns) and metastatic (black columns) melanoma in the presence of CA-074 (left panel), Pepstatin A (central panel) or cathepsin L inhibitor (right panel). Dotted lines represent the mean of the results obtained in primary melanoma cell lines, and dashed lines indicate the mean of the results obtained in metastatic melanoma cell lines by using DMSO (vehicle of the cathepsin inhibitors). ( C ) Invasion test on two representative cell lines from primary (white columns) and metastatic (black columns) melanoma in the absence or in the presence of specific antibodies against cathepsin B (left panel), cathepsin D (central panel) or cathepsin L (right panel). Dotted lines represent the mean of the results obtained in primary melanoma cell lines, and dashed lines indicate the mean of the results obtained in metastatic melanoma cell lines by using IgG1 as positive control. ( D ) Histogram showing the results obtained from four different primary melanoma (white columns) or metastatic melanoma (black columns) cell lines in the absence or in the presence of CA-074 or antibodies against cathepsin B. Data are reported as mean ± SD of the percentage of invading cells. Student's t -test indicates: p = 0.0097 for untreated MM cells vs. CA-074-treated MM cells and p = 0.0030 for untreated MM cells vs. MM cells treated with anti-cathepsin B antibodies.

    Journal: Molecular Cancer

    Article Title: Cathepsin B inhibition interferes with metastatic potential of human melanoma: an in vitro and in vivo study

    doi: 10.1186/1476-4598-9-207

    Figure Lengend Snippet: Cathepsins and in vitro invasiveness: effect of chemical and biological inhibitors . ( A ) Invasion test on primary (white columns) and metastatic (black columns) melanoma cell lines. ( B ) Invasion test on two representative cell lines from primary (white columns) and metastatic (black columns) melanoma in the presence of CA-074 (left panel), Pepstatin A (central panel) or cathepsin L inhibitor (right panel). Dotted lines represent the mean of the results obtained in primary melanoma cell lines, and dashed lines indicate the mean of the results obtained in metastatic melanoma cell lines by using DMSO (vehicle of the cathepsin inhibitors). ( C ) Invasion test on two representative cell lines from primary (white columns) and metastatic (black columns) melanoma in the absence or in the presence of specific antibodies against cathepsin B (left panel), cathepsin D (central panel) or cathepsin L (right panel). Dotted lines represent the mean of the results obtained in primary melanoma cell lines, and dashed lines indicate the mean of the results obtained in metastatic melanoma cell lines by using IgG1 as positive control. ( D ) Histogram showing the results obtained from four different primary melanoma (white columns) or metastatic melanoma (black columns) cell lines in the absence or in the presence of CA-074 or antibodies against cathepsin B. Data are reported as mean ± SD of the percentage of invading cells. Student's t -test indicates: p = 0.0097 for untreated MM cells vs. CA-074-treated MM cells and p = 0.0030 for untreated MM cells vs. MM cells treated with anti-cathepsin B antibodies.

    Article Snippet: For cell migration and invasion assays, cells were seeded in the presence of the cathepsin D inhibitor (Pepstatin A, 100 μM, Calbiochem), the cathepsin L inhibitor (cathepsin L inhibitor II, Z-FY-CHO, 10 μM, Calbiochem) as well as two cathepsin B inhibitors: CA-074 (cell unpermeant) and CA-074Me (cell permeant) (both given at a concentration of 10 μM, Calbiochem, Nottingham, UK).

    Techniques: In Vitro, Positive Control

    Cathepsin B expression in cells from primary and metastatic human melanomas . Western blot analysis (left panels) of cathepsin B, cathepsin D, cathepsin L and tubulin in the primary melanoma cell lines PM1 ( A ) and PM2 ( B) and in the metastatic melanoma cell lines MM1 ( A ) and MM2 ( B ). Quantitative flow cytometry analysis (right panels) of plasma membrane cathepsin B in PM1 ( A ) and PM2 ( B ) cell lines and in MM1 ( A ) and MM2 ( B ) cell lines. The values of Western blot signals, reported in the bottom panels of ( A ) and ( B ), were obtained by densitometric analysis and expressed as arbitrary units (a.u.). Numbers in the right panels of ( A ) and ( B ) represent the median fluorescence values. In the right panels of ( A ) and ( B ) statistical analysis performed by non-parametric K/S test is reported.

    Journal: Molecular Cancer

    Article Title: Cathepsin B inhibition interferes with metastatic potential of human melanoma: an in vitro and in vivo study

    doi: 10.1186/1476-4598-9-207

    Figure Lengend Snippet: Cathepsin B expression in cells from primary and metastatic human melanomas . Western blot analysis (left panels) of cathepsin B, cathepsin D, cathepsin L and tubulin in the primary melanoma cell lines PM1 ( A ) and PM2 ( B) and in the metastatic melanoma cell lines MM1 ( A ) and MM2 ( B ). Quantitative flow cytometry analysis (right panels) of plasma membrane cathepsin B in PM1 ( A ) and PM2 ( B ) cell lines and in MM1 ( A ) and MM2 ( B ) cell lines. The values of Western blot signals, reported in the bottom panels of ( A ) and ( B ), were obtained by densitometric analysis and expressed as arbitrary units (a.u.). Numbers in the right panels of ( A ) and ( B ) represent the median fluorescence values. In the right panels of ( A ) and ( B ) statistical analysis performed by non-parametric K/S test is reported.

    Article Snippet: For cell migration and invasion assays, cells were seeded in the presence of the cathepsin D inhibitor (Pepstatin A, 100 μM, Calbiochem), the cathepsin L inhibitor (cathepsin L inhibitor II, Z-FY-CHO, 10 μM, Calbiochem) as well as two cathepsin B inhibitors: CA-074 (cell unpermeant) and CA-074Me (cell permeant) (both given at a concentration of 10 μM, Calbiochem, Nottingham, UK).

    Techniques: Expressing, Western Blot, Flow Cytometry, Cytometry, Fluorescence