cassettes hybridization Search Results


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  • 99
    Thermo Fisher ecori
    Southern hybridisation of total DNA from transgenic cassava cv.60444 transformed with SACMV AC1AC4 construct, digested with restriction enzymes <t>EcoRI</t> and <t>HindIII,</t> and hybridized with DNA probe specific to Hyg gene. Lane M: DIG-labelled molecular weight parker (ThermoFisher Scientific), lane 1-16: independent transgenic cassava lines. Purified binary vector pC1305.1-AC1/AC4 and wild-type untransformed cv.60444 were included as positive (+) and negative (-) controls respectively.
    Ecori, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 9775 article reviews
    Price from $9.99 to $1999.99
    ecori - by Bioz Stars, 2020-04
    99/100 stars
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    99
    Millipore simvastatin
    <t>Simvastatin</t> prevents endothelial apoptosis during cold storage through a KLF2-dependent mechanism A) Expression of the apoptosis marker Annexin V in EC exposed for 24h to vasoprotective flow and statically cold stored for 0h or 6h in University of Wisconsin Solution supplemented with simvastatin or its vehicle (* p
    Simvastatin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1452 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1452 article reviews
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    86
    TeleChem International hybridization cassette
    <t>Simvastatin</t> prevents endothelial apoptosis during cold storage through a KLF2-dependent mechanism A) Expression of the apoptosis marker Annexin V in EC exposed for 24h to vasoprotective flow and statically cold stored for 0h or 6h in University of Wisconsin Solution supplemented with simvastatin or its vehicle (* p
    Hybridization Cassette, supplied by TeleChem International, used in various techniques. Bioz Stars score: 86/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybridization cassette/product/TeleChem International
    Average 86 stars, based on 22 article reviews
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    86
    TeleChem International arrayit hybridization cassettes
    <t>Simvastatin</t> prevents endothelial apoptosis during cold storage through a KLF2-dependent mechanism A) Expression of the apoptosis marker Annexin V in EC exposed for 24h to vasoprotective flow and statically cold stored for 0h or 6h in University of Wisconsin Solution supplemented with simvastatin or its vehicle (* p
    Arrayit Hybridization Cassettes, supplied by TeleChem International, used in various techniques. Bioz Stars score: 86/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arrayit hybridization cassettes/product/TeleChem International
    Average 86 stars, based on 23 article reviews
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    85
    Caliper Life Sciences caliper hybridization cassette
    <t>Simvastatin</t> prevents endothelial apoptosis during cold storage through a KLF2-dependent mechanism A) Expression of the apoptosis marker Annexin V in EC exposed for 24h to vasoprotective flow and statically cold stored for 0h or 6h in University of Wisconsin Solution supplemented with simvastatin or its vehicle (* p
    Caliper Hybridization Cassette, supplied by Caliper Life Sciences, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caliper hybridization cassette/product/Caliper Life Sciences
    Average 85 stars, based on 4 article reviews
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    95
    Arrayit Corporation microarray hybridization cassettes
    Reproducibility and stability of analyses on Ct whole-proteome microarrays. Correlation of MFI values obtained in two independent experiments was determined by linear regression analysis (forced through the origin) and is expressed by the slope and Pearson’s correlation coefficient (r). Cut-offs are highlighted by dashed blue lines. ( a ) Expression staining using antibodies against fusion tags of the proteins was replicated on two slides from the same production batch. Each dot represents one Ct protein with anti-V5 MFI values from two independent experiments. ( b ) Repetition of an immunoassay for the same pool of five sera on two slides produced within the same batch. ( c ) Same as b, with slides from two different production batches (same pool as in panel b). ( d ) Repetition of an immunoassay for the same pool of five sera on two slides (different pool from panels b and c). One Immunoassay was performed shortly after production of the <t>microarray</t> and one slide was stored for a time period of 3 months before the assay was performed.
    Microarray Hybridization Cassettes, supplied by Arrayit Corporation, used in various techniques. Bioz Stars score: 95/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 19 article reviews
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    93
    Thermo Fisher glassarray hybridization cassettes
    Reproducibility and stability of analyses on Ct whole-proteome microarrays. Correlation of MFI values obtained in two independent experiments was determined by linear regression analysis (forced through the origin) and is expressed by the slope and Pearson’s correlation coefficient (r). Cut-offs are highlighted by dashed blue lines. ( a ) Expression staining using antibodies against fusion tags of the proteins was replicated on two slides from the same production batch. Each dot represents one Ct protein with anti-V5 MFI values from two independent experiments. ( b ) Repetition of an immunoassay for the same pool of five sera on two slides produced within the same batch. ( c ) Same as b, with slides from two different production batches (same pool as in panel b). ( d ) Repetition of an immunoassay for the same pool of five sera on two slides (different pool from panels b and c). One Immunoassay was performed shortly after production of the <t>microarray</t> and one slide was stored for a time period of 3 months before the assay was performed.
    Glassarray Hybridization Cassettes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 53 article reviews
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    85
    Corning Life Sciences humidified hybridization cassette
    Reproducibility and stability of analyses on Ct whole-proteome microarrays. Correlation of MFI values obtained in two independent experiments was determined by linear regression analysis (forced through the origin) and is expressed by the slope and Pearson’s correlation coefficient (r). Cut-offs are highlighted by dashed blue lines. ( a ) Expression staining using antibodies against fusion tags of the proteins was replicated on two slides from the same production batch. Each dot represents one Ct protein with anti-V5 MFI values from two independent experiments. ( b ) Repetition of an immunoassay for the same pool of five sera on two slides produced within the same batch. ( c ) Same as b, with slides from two different production batches (same pool as in panel b). ( d ) Repetition of an immunoassay for the same pool of five sera on two slides (different pool from panels b and c). One Immunoassay was performed shortly after production of the <t>microarray</t> and one slide was stored for a time period of 3 months before the assay was performed.
    Humidified Hybridization Cassette, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 6 article reviews
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    86
    Capital Biosciences microarray hybridization cassette
    Reproducibility and stability of analyses on Ct whole-proteome microarrays. Correlation of MFI values obtained in two independent experiments was determined by linear regression analysis (forced through the origin) and is expressed by the slope and Pearson’s correlation coefficient (r). Cut-offs are highlighted by dashed blue lines. ( a ) Expression staining using antibodies against fusion tags of the proteins was replicated on two slides from the same production batch. Each dot represents one Ct protein with anti-V5 MFI values from two independent experiments. ( b ) Repetition of an immunoassay for the same pool of five sera on two slides produced within the same batch. ( c ) Same as b, with slides from two different production batches (same pool as in panel b). ( d ) Repetition of an immunoassay for the same pool of five sera on two slides (different pool from panels b and c). One Immunoassay was performed shortly after production of the <t>microarray</t> and one slide was stored for a time period of 3 months before the assay was performed.
    Microarray Hybridization Cassette, supplied by Capital Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 6 article reviews
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    86/100 stars
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    85
    TeleChem International arrayit hybridization cassette chamber
    Reproducibility and stability of analyses on Ct whole-proteome microarrays. Correlation of MFI values obtained in two independent experiments was determined by linear regression analysis (forced through the origin) and is expressed by the slope and Pearson’s correlation coefficient (r). Cut-offs are highlighted by dashed blue lines. ( a ) Expression staining using antibodies against fusion tags of the proteins was replicated on two slides from the same production batch. Each dot represents one Ct protein with anti-V5 MFI values from two independent experiments. ( b ) Repetition of an immunoassay for the same pool of five sera on two slides produced within the same batch. ( c ) Same as b, with slides from two different production batches (same pool as in panel b). ( d ) Repetition of an immunoassay for the same pool of five sera on two slides (different pool from panels b and c). One Immunoassay was performed shortly after production of the <t>microarray</t> and one slide was stored for a time period of 3 months before the assay was performed.
    Arrayit Hybridization Cassette Chamber, supplied by TeleChem International, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arrayit hybridization cassette chamber/product/TeleChem International
    Average 85 stars, based on 4 article reviews
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    86
    Arrayit Corporation ahc1x24 microarray hybridization cassette
    Reproducibility and stability of analyses on Ct whole-proteome microarrays. Correlation of MFI values obtained in two independent experiments was determined by linear regression analysis (forced through the origin) and is expressed by the slope and Pearson’s correlation coefficient (r). Cut-offs are highlighted by dashed blue lines. ( a ) Expression staining using antibodies against fusion tags of the proteins was replicated on two slides from the same production batch. Each dot represents one Ct protein with anti-V5 MFI values from two independent experiments. ( b ) Repetition of an immunoassay for the same pool of five sera on two slides produced within the same batch. ( c ) Same as b, with slides from two different production batches (same pool as in panel b). ( d ) Repetition of an immunoassay for the same pool of five sera on two slides (different pool from panels b and c). One Immunoassay was performed shortly after production of the <t>microarray</t> and one slide was stored for a time period of 3 months before the assay was performed.
    Ahc1x24 Microarray Hybridization Cassette, supplied by Arrayit Corporation, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ahc1x24 microarray hybridization cassette/product/Arrayit Corporation
    Average 86 stars, based on 4 article reviews
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    95
    Arrayit Corporation multi well hybridization cassettes
    Reproducibility and stability of analyses on Ct whole-proteome microarrays. Correlation of MFI values obtained in two independent experiments was determined by linear regression analysis (forced through the origin) and is expressed by the slope and Pearson’s correlation coefficient (r). Cut-offs are highlighted by dashed blue lines. ( a ) Expression staining using antibodies against fusion tags of the proteins was replicated on two slides from the same production batch. Each dot represents one Ct protein with anti-V5 MFI values from two independent experiments. ( b ) Repetition of an immunoassay for the same pool of five sera on two slides produced within the same batch. ( c ) Same as b, with slides from two different production batches (same pool as in panel b). ( d ) Repetition of an immunoassay for the same pool of five sera on two slides (different pool from panels b and c). One Immunoassay was performed shortly after production of the <t>microarray</t> and one slide was stored for a time period of 3 months before the assay was performed.
    Multi Well Hybridization Cassettes, supplied by Arrayit Corporation, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TeleChem International microarray hybridization cassettes
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    Microarray Hybridization Cassettes, supplied by TeleChem International, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 6 article reviews
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    80
    Corning Life Sciences hybridization cassette plate
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    Hybridization Cassette Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 1 article reviews
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    86
    TeleChem International 24 well hybridization cassette
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    24 Well Hybridization Cassette, supplied by TeleChem International, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 7 article reviews
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    24 well hybridization cassette - by Bioz Stars, 2020-04
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    85
    Corning Life Sciences light protected sealed corning hybridization cassettes
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    Light Protected Sealed Corning Hybridization Cassettes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Arrayit Corporation multi well 1 × 16 microarray hybridization cassettes
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    Multi Well 1 × 16 Microarray Hybridization Cassettes, supplied by Arrayit Corporation, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 2 article reviews
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    90
    Arrayit Corporation 24 well arrayit multi well hybridization cassette
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    24 Well Arrayit Multi Well Hybridization Cassette, supplied by Arrayit Corporation, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/24 well arrayit multi well hybridization cassette/product/Arrayit Corporation
    Average 90 stars, based on 2 article reviews
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    86
    TeleChem International hybridisation cassette
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    Hybridisation Cassette, supplied by TeleChem International, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybridisation cassette/product/TeleChem International
    Average 86 stars, based on 6 article reviews
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    84
    Unigene mouse cassette as events
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    Mouse Cassette As Events, supplied by Unigene, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 2 article reviews
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    97
    Kodak Corp biomax mr film
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    Biomax Mr Film, supplied by Kodak Corp, used in various techniques. Bioz Stars score: 97/100, based on 3313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 3313 article reviews
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    88
    Illumina Inc humanref 8 v2 expression beadchip
    Construction of a T. rubrum cDNA <t>microarray</t> for the specific analysis of protein digestion.
    Humanref 8 V2 Expression Beadchip, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 46 article reviews
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    85
    TeleChem International microarray hybridization chamber
    RNA Gel Blot Analysis to Confirm DNA <t>Microarray</t> Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .
    Microarray Hybridization Chamber, supplied by TeleChem International, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    TeleChem International cassettes
    RNA Gel Blot Analysis to Confirm DNA <t>Microarray</t> Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .
    Cassettes, supplied by TeleChem International, used in various techniques. Bioz Stars score: 87/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 9 article reviews
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    92
    MOgene genechip mogene 1 0 st v1 cassettes
    RNA Gel Blot Analysis to Confirm DNA <t>Microarray</t> Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .
    Genechip Mogene 1 0 St V1 Cassettes, supplied by MOgene, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Sakura Finetek vip tissue tek enclosed processor
    RNA Gel Blot Analysis to Confirm DNA <t>Microarray</t> Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .
    Vip Tissue Tek Enclosed Processor, supplied by Sakura Finetek, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    88
    GE Healthcare phosphor screen cassette
    RNA Gel Blot Analysis to Confirm DNA <t>Microarray</t> Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .
    Phosphor Screen Cassette, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 33 article reviews
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    93
    GE Healthcare phosphorimager cassettes
    RNA Gel Blot Analysis to Confirm DNA <t>Microarray</t> Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .
    Phosphorimager Cassettes, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 68 article reviews
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    87
    Kodak Corp x ray cassettes
    RNA Gel Blot Analysis to Confirm DNA <t>Microarray</t> Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .
    X Ray Cassettes, supplied by Kodak Corp, used in various techniques. Bioz Stars score: 87/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 17 article reviews
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    95
    FUJIFILM phosphor imaging plates
    RNA Gel Blot Analysis to Confirm DNA <t>Microarray</t> Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .
    Phosphor Imaging Plates, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 95/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 203 article reviews
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    87
    Millipore fast frame cassette
    Sensitivity comparison between the <t>FAST</t> ® Frame Cassette and the <t>Hybrislip™.</t> ( A ) Different amounts (pmoles) of the same target, generated by the modified PCR method are plotted against the mean signal-background (RFU) for hybridization carried out in the FAST ® Frame hybridization chamber. ( B ) Different amounts (pmoles) of the same target, generated by the modified PCR method, are plotted against the mean signal-background (RFU) for hybridization carried out using the Hybrislip™.
    Fast Frame Cassette, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 8 article reviews
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    89
    Analytik Jena AG hb 1000 hybridization oven
    Sensitivity comparison between the <t>FAST</t> ® Frame Cassette and the <t>Hybrislip™.</t> ( A ) Different amounts (pmoles) of the same target, generated by the modified PCR method are plotted against the mean signal-background (RFU) for hybridization carried out in the FAST ® Frame hybridization chamber. ( B ) Different amounts (pmoles) of the same target, generated by the modified PCR method, are plotted against the mean signal-background (RFU) for hybridization carried out using the Hybrislip™.
    Hb 1000 Hybridization Oven, supplied by Analytik Jena AG, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 7 article reviews
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    Southern hybridisation of total DNA from transgenic cassava cv.60444 transformed with SACMV AC1AC4 construct, digested with restriction enzymes EcoRI and HindIII, and hybridized with DNA probe specific to Hyg gene. Lane M: DIG-labelled molecular weight parker (ThermoFisher Scientific), lane 1-16: independent transgenic cassava lines. Purified binary vector pC1305.1-AC1/AC4 and wild-type untransformed cv.60444 were included as positive (+) and negative (-) controls respectively.

    Journal: Biotechnology Reports

    Article Title: RNA silencing of South African cassava mosaic virus in transgenic cassava expressing AC1/AC4 hp- RNA induces tolerance

    doi: 10.1016/j.btre.2019.e00383

    Figure Lengend Snippet: Southern hybridisation of total DNA from transgenic cassava cv.60444 transformed with SACMV AC1AC4 construct, digested with restriction enzymes EcoRI and HindIII, and hybridized with DNA probe specific to Hyg gene. Lane M: DIG-labelled molecular weight parker (ThermoFisher Scientific), lane 1-16: independent transgenic cassava lines. Purified binary vector pC1305.1-AC1/AC4 and wild-type untransformed cv.60444 were included as positive (+) and negative (-) controls respectively.

    Article Snippet: Twenty micrograms of DNA from each line was digested overnight with HindIII (15053–15059) which cuts once in the cassette, and EcoRI, restriction enzymes (ThermoFisher Scientific) and separated using gel electrophoresis, in a 1% agarose gel in 1X TAE.

    Techniques: Hybridization, Transgenic Assay, Transformation Assay, Construct, Molecular Weight, Purification, Plasmid Preparation

    Simvastatin prevents endothelial apoptosis during cold storage through a KLF2-dependent mechanism A) Expression of the apoptosis marker Annexin V in EC exposed for 24h to vasoprotective flow and statically cold stored for 0h or 6h in University of Wisconsin Solution supplemented with simvastatin or its vehicle (* p

    Journal: Transplantation

    Article Title: Flow Cessation triggers Endothelial Dysfunction during Organ Cold Storage Conditions: Strategies for Pharmacologic Intervention

    doi: 10.1097/TP.0b013e3181e228db

    Figure Lengend Snippet: Simvastatin prevents endothelial apoptosis during cold storage through a KLF2-dependent mechanism A) Expression of the apoptosis marker Annexin V in EC exposed for 24h to vasoprotective flow and statically cold stored for 0h or 6h in University of Wisconsin Solution supplemented with simvastatin or its vehicle (* p

    Article Snippet: After 24h of vasoprotective flow stimulation, EC were washed twice with phosphate buffered saline (PBS) and incubated under static conditions for different periods of time at 37°C in complete EC culture media or at 4°C in UWS supplemented with Simvastatin (Calbiochem) or its vehicle DMSO (0.1% v/v).

    Techniques: Expressing, Marker, Flow Cytometry

    Simvastatin maintains endothelial KLF2 and its vasoprotective target genes expression during static cold storage A) Left , KLF2 mRNA expression determined in EC exposed for 24h to vasoprotective flow followed by static preservation at 4°C for 0h, 1h, 3h and 6h in University of Wisconsin solution supplemented with simvastatin (1μM) or its vehicle. Right , representative western blot of KLF2 and α-tubulin protein expression determined in EC under indicated conditions. B) Expression of KLF2-dependent vasoprotective target genes determined in EC described above (*p

    Journal: Transplantation

    Article Title: Flow Cessation triggers Endothelial Dysfunction during Organ Cold Storage Conditions: Strategies for Pharmacologic Intervention

    doi: 10.1097/TP.0b013e3181e228db

    Figure Lengend Snippet: Simvastatin maintains endothelial KLF2 and its vasoprotective target genes expression during static cold storage A) Left , KLF2 mRNA expression determined in EC exposed for 24h to vasoprotective flow followed by static preservation at 4°C for 0h, 1h, 3h and 6h in University of Wisconsin solution supplemented with simvastatin (1μM) or its vehicle. Right , representative western blot of KLF2 and α-tubulin protein expression determined in EC under indicated conditions. B) Expression of KLF2-dependent vasoprotective target genes determined in EC described above (*p

    Article Snippet: After 24h of vasoprotective flow stimulation, EC were washed twice with phosphate buffered saline (PBS) and incubated under static conditions for different periods of time at 37°C in complete EC culture media or at 4°C in UWS supplemented with Simvastatin (Calbiochem) or its vehicle DMSO (0.1% v/v).

    Techniques: Expressing, Flow Cytometry, Preserving, Western Blot

    Simvastatin preserves the endothelial anti-inflammatory phenotype upon cold storage through a KLF2-dependent mechanism A) E-Selectin ( top ) and VCAM-1 ( bottom ) mRNA expression determined in EC expose to vasoprotective flow for 24h and statically cold preserved for 0h or 6h with simvastatin or its vehicle, and afterwards stimulated for 4h with IL-1β (0.1U/mL) in warm culture conditions (n=3). B) Top, representative flow cytometry histogram of E-Selectin and VCAM-1 cell surface expression determined in EC described above. Bottom, flow cytometry quantitative analysis of four independent experiments (*p

    Journal: Transplantation

    Article Title: Flow Cessation triggers Endothelial Dysfunction during Organ Cold Storage Conditions: Strategies for Pharmacologic Intervention

    doi: 10.1097/TP.0b013e3181e228db

    Figure Lengend Snippet: Simvastatin preserves the endothelial anti-inflammatory phenotype upon cold storage through a KLF2-dependent mechanism A) E-Selectin ( top ) and VCAM-1 ( bottom ) mRNA expression determined in EC expose to vasoprotective flow for 24h and statically cold preserved for 0h or 6h with simvastatin or its vehicle, and afterwards stimulated for 4h with IL-1β (0.1U/mL) in warm culture conditions (n=3). B) Top, representative flow cytometry histogram of E-Selectin and VCAM-1 cell surface expression determined in EC described above. Bottom, flow cytometry quantitative analysis of four independent experiments (*p

    Article Snippet: After 24h of vasoprotective flow stimulation, EC were washed twice with phosphate buffered saline (PBS) and incubated under static conditions for different periods of time at 37°C in complete EC culture media or at 4°C in UWS supplemented with Simvastatin (Calbiochem) or its vehicle DMSO (0.1% v/v).

    Techniques: Expressing, Flow Cytometry, Cytometry

    Simvastatin inhibits endothelial activation after cold storage Top , EC monolayers were cultured for 24h under vasoprotective flow, followed by the immediate addition of IL-1β 0.1 U/mL, 4h, 37°C, left), or by cold storage for 6h with simvastatin (1 μM, right) or its vehicle (center), and then treated for 4h with IL-1β (as above). All groups were then incubated with fluorescently labeled HL-60 cells. Shown are representative fields of EC monolayers (gray) and attached HL60 cells (yellow). Bottom , quantitative fluorescence analysis of bound HL-60 cells (*p

    Journal: Transplantation

    Article Title: Flow Cessation triggers Endothelial Dysfunction during Organ Cold Storage Conditions: Strategies for Pharmacologic Intervention

    doi: 10.1097/TP.0b013e3181e228db

    Figure Lengend Snippet: Simvastatin inhibits endothelial activation after cold storage Top , EC monolayers were cultured for 24h under vasoprotective flow, followed by the immediate addition of IL-1β 0.1 U/mL, 4h, 37°C, left), or by cold storage for 6h with simvastatin (1 μM, right) or its vehicle (center), and then treated for 4h with IL-1β (as above). All groups were then incubated with fluorescently labeled HL-60 cells. Shown are representative fields of EC monolayers (gray) and attached HL60 cells (yellow). Bottom , quantitative fluorescence analysis of bound HL-60 cells (*p

    Article Snippet: After 24h of vasoprotective flow stimulation, EC were washed twice with phosphate buffered saline (PBS) and incubated under static conditions for different periods of time at 37°C in complete EC culture media or at 4°C in UWS supplemented with Simvastatin (Calbiochem) or its vehicle DMSO (0.1% v/v).

    Techniques: Activation Assay, Cell Culture, Flow Cytometry, Incubation, Labeling, Fluorescence

    Simvastatin maintains the expression of endothelial KLF2 and its vasoprotective target genes during cold storage ex vivo A) KLF2 mRNA localization determined by in situ hybridization in murine aorta. B) KLF2 and its target genes eNOS (C) and thrombomodulin (D) mRNA expression in murine aortas cold preserved for 0h, 6h or 24h in University of Wisconsin solution supplemented with simvastatin (10μM) or its vehicle (*p

    Journal: Transplantation

    Article Title: Flow Cessation triggers Endothelial Dysfunction during Organ Cold Storage Conditions: Strategies for Pharmacologic Intervention

    doi: 10.1097/TP.0b013e3181e228db

    Figure Lengend Snippet: Simvastatin maintains the expression of endothelial KLF2 and its vasoprotective target genes during cold storage ex vivo A) KLF2 mRNA localization determined by in situ hybridization in murine aorta. B) KLF2 and its target genes eNOS (C) and thrombomodulin (D) mRNA expression in murine aortas cold preserved for 0h, 6h or 24h in University of Wisconsin solution supplemented with simvastatin (10μM) or its vehicle (*p

    Article Snippet: After 24h of vasoprotective flow stimulation, EC were washed twice with phosphate buffered saline (PBS) and incubated under static conditions for different periods of time at 37°C in complete EC culture media or at 4°C in UWS supplemented with Simvastatin (Calbiochem) or its vehicle DMSO (0.1% v/v).

    Techniques: Expressing, Ex Vivo, In Situ Hybridization

    Reproducibility and stability of analyses on Ct whole-proteome microarrays. Correlation of MFI values obtained in two independent experiments was determined by linear regression analysis (forced through the origin) and is expressed by the slope and Pearson’s correlation coefficient (r). Cut-offs are highlighted by dashed blue lines. ( a ) Expression staining using antibodies against fusion tags of the proteins was replicated on two slides from the same production batch. Each dot represents one Ct protein with anti-V5 MFI values from two independent experiments. ( b ) Repetition of an immunoassay for the same pool of five sera on two slides produced within the same batch. ( c ) Same as b, with slides from two different production batches (same pool as in panel b). ( d ) Repetition of an immunoassay for the same pool of five sera on two slides (different pool from panels b and c). One Immunoassay was performed shortly after production of the microarray and one slide was stored for a time period of 3 months before the assay was performed.

    Journal: Scientific Reports

    Article Title: Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression

    doi: 10.1038/s41598-018-25918-3

    Figure Lengend Snippet: Reproducibility and stability of analyses on Ct whole-proteome microarrays. Correlation of MFI values obtained in two independent experiments was determined by linear regression analysis (forced through the origin) and is expressed by the slope and Pearson’s correlation coefficient (r). Cut-offs are highlighted by dashed blue lines. ( a ) Expression staining using antibodies against fusion tags of the proteins was replicated on two slides from the same production batch. Each dot represents one Ct protein with anti-V5 MFI values from two independent experiments. ( b ) Repetition of an immunoassay for the same pool of five sera on two slides produced within the same batch. ( c ) Same as b, with slides from two different production batches (same pool as in panel b). ( d ) Repetition of an immunoassay for the same pool of five sera on two slides (different pool from panels b and c). One Immunoassay was performed shortly after production of the microarray and one slide was stored for a time period of 3 months before the assay was performed.

    Article Snippet: The slides were then incubated in microarray hybridization cassettes (Arrayit Corporation) at 37 °C for 1 hour and at 30 °C overnight in a humidified environment.

    Techniques: Expressing, Staining, Produced, Microarray

    Microarray displaying the whole-proteome of Ct. ( a ) Results of the determination of on-chip protein expression using antibody staining against the terminal tags (green signal: anti-V5 antibody; red signal: anti-His antibody; yellow signal: overlay of both signals). All 898 proteins were spotted onto one array. The last 22 spots indicated by red and white frames represent the negative controls NC1 and NC2, respectively. The upper part of the array comprised genes of interest (GOI) with a DNA template length of up to 900 bp. ( b ) Final MFI values obtained by the anti-V5 antibody were sorted in descending order and plotted against the overall number of Ct proteins. The calculated cut-off (100 MFI) is represented by a red line, indicating that less than 10% of all proteins are below the cut-off and are therefore considered not to be expressed in full length.

    Journal: Scientific Reports

    Article Title: Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression

    doi: 10.1038/s41598-018-25918-3

    Figure Lengend Snippet: Microarray displaying the whole-proteome of Ct. ( a ) Results of the determination of on-chip protein expression using antibody staining against the terminal tags (green signal: anti-V5 antibody; red signal: anti-His antibody; yellow signal: overlay of both signals). All 898 proteins were spotted onto one array. The last 22 spots indicated by red and white frames represent the negative controls NC1 and NC2, respectively. The upper part of the array comprised genes of interest (GOI) with a DNA template length of up to 900 bp. ( b ) Final MFI values obtained by the anti-V5 antibody were sorted in descending order and plotted against the overall number of Ct proteins. The calculated cut-off (100 MFI) is represented by a red line, indicating that less than 10% of all proteins are below the cut-off and are therefore considered not to be expressed in full length.

    Article Snippet: The slides were then incubated in microarray hybridization cassettes (Arrayit Corporation) at 37 °C for 1 hour and at 30 °C overnight in a humidified environment.

    Techniques: Microarray, Chromatin Immunoprecipitation, Expressing, Staining

    Antibody patterns in individual sera with 130 selected Ct antigens. ( a ) Each microarray comprised 8 blocks and each block contained the same 130 Ct antigens and 10 negative controls (NC1). Individual blocks were incubated with serum from a Ct-uninfected woman (topmost block), and seven Ct-infected cervical cancer patients. ( b ) Enlarged view of antibody reactivity patterns in single sera from Ct-infected women, the top two being cancer-free and the bottom two with cervical cancer. Boxed spots highlight potential markers for general Ct infection (green) and cervical cancer (blue).

    Journal: Scientific Reports

    Article Title: Immunoprofiling of Chlamydia trachomatis using whole-proteome microarrays generated by on-chip in situ expression

    doi: 10.1038/s41598-018-25918-3

    Figure Lengend Snippet: Antibody patterns in individual sera with 130 selected Ct antigens. ( a ) Each microarray comprised 8 blocks and each block contained the same 130 Ct antigens and 10 negative controls (NC1). Individual blocks were incubated with serum from a Ct-uninfected woman (topmost block), and seven Ct-infected cervical cancer patients. ( b ) Enlarged view of antibody reactivity patterns in single sera from Ct-infected women, the top two being cancer-free and the bottom two with cervical cancer. Boxed spots highlight potential markers for general Ct infection (green) and cervical cancer (blue).

    Article Snippet: The slides were then incubated in microarray hybridization cassettes (Arrayit Corporation) at 37 °C for 1 hour and at 30 °C overnight in a humidified environment.

    Techniques: Microarray, Blocking Assay, Incubation, Infection

    Construction of a T. rubrum cDNA microarray for the specific analysis of protein digestion.

    Journal:

    Article Title: Gene Expression Profiling in the Human Pathogenic Dermatophyte Trichophyton rubrum during Growth on Proteins ▿ during Growth on Proteins ▿ †

    doi: 10.1128/EC.00208-08

    Figure Lengend Snippet: Construction of a T. rubrum cDNA microarray for the specific analysis of protein digestion.

    Article Snippet: Hybridizations were performed overnight at 65°C in microarray hybridization cassettes (TeleChem International Inc.).

    Techniques: Microarray

    Microarray construction.

    Journal:

    Article Title: Gene Expression Profiling in the Human Pathogenic Dermatophyte Trichophyton rubrum during Growth on Proteins ▿ during Growth on Proteins ▿ †

    doi: 10.1128/EC.00208-08

    Figure Lengend Snippet: Microarray construction.

    Article Snippet: Hybridizations were performed overnight at 65°C in microarray hybridization cassettes (TeleChem International Inc.).

    Techniques: Microarray

    Validation of microarray data by quantitative real-time RT-PCR.

    Journal:

    Article Title: Gene Expression Profiling in the Human Pathogenic Dermatophyte Trichophyton rubrum during Growth on Proteins ▿ during Growth on Proteins ▿ †

    doi: 10.1128/EC.00208-08

    Figure Lengend Snippet: Validation of microarray data by quantitative real-time RT-PCR.

    Article Snippet: Hybridizations were performed overnight at 65°C in microarray hybridization cassettes (TeleChem International Inc.).

    Techniques: Microarray, Quantitative RT-PCR

    RNA Gel Blot Analysis to Confirm DNA Microarray Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .

    Journal: The Plant Cell

    Article Title: New Molecular Phenotypes in the dst Mutants of Arabidopsis Revealed by DNA Microarray Analysis

    doi: 10.1105/tpc.010295

    Figure Lengend Snippet: RNA Gel Blot Analysis to Confirm DNA Microarray Data. Lanes contained 10 μg of total RNA extracted from wild-type (1519), dst1 , and dst2 plants. Each blot was hybridized sequentially with 32 P-labeled eIF4A and then with HPH (A) , RH15 (B) , and SAUR-AC1 and SAUR-like1 (C) .

    Article Snippet: The slide was placed in a microarray hybridization chamber (ArrayIt Hybridization Cassette; Telechem International) with 200 μL of 3 × SSC to ensure high-humidity conditions.

    Techniques: Western Blot, Microarray, Labeling

    Assessment of the Reproducibility of the Microarray Data Using Fewer Replicates. (A) Histogram plot comparing the percentage of reproducible and nonreproducible clones. The percentage of clones ( y axis) is plotted against slide pairs ( x axis) used for the microarray experiments. Each slide pair denotes microarray slides that were technical replicas (i.e., reciprocal labeling was performed with each pair of targets). The slide numbers denote the ExptID in the SMD. (B) Graph showing the percentage of nonreproducible clones ( y axis) plotted against the number of slides used for the microarray analysis ( x axis).

    Journal: The Plant Cell

    Article Title: New Molecular Phenotypes in the dst Mutants of Arabidopsis Revealed by DNA Microarray Analysis

    doi: 10.1105/tpc.010295

    Figure Lengend Snippet: Assessment of the Reproducibility of the Microarray Data Using Fewer Replicates. (A) Histogram plot comparing the percentage of reproducible and nonreproducible clones. The percentage of clones ( y axis) is plotted against slide pairs ( x axis) used for the microarray experiments. Each slide pair denotes microarray slides that were technical replicas (i.e., reciprocal labeling was performed with each pair of targets). The slide numbers denote the ExptID in the SMD. (B) Graph showing the percentage of nonreproducible clones ( y axis) plotted against the number of slides used for the microarray analysis ( x axis).

    Article Snippet: The slide was placed in a microarray hybridization chamber (ArrayIt Hybridization Cassette; Telechem International) with 200 μL of 3 × SSC to ensure high-humidity conditions.

    Techniques: Microarray, Clone Assay, Labeling

    Sensitivity comparison between the FAST ® Frame Cassette and the Hybrislip™. ( A ) Different amounts (pmoles) of the same target, generated by the modified PCR method are plotted against the mean signal-background (RFU) for hybridization carried out in the FAST ® Frame hybridization chamber. ( B ) Different amounts (pmoles) of the same target, generated by the modified PCR method, are plotted against the mean signal-background (RFU) for hybridization carried out using the Hybrislip™.

    Journal: The Open Biochemistry Journal

    Article Title: Effect of Target Length on Specificity and Sensitivity of Oligonucleotide Microarrays: A Comparison between Dendrimer and Modified PCR based Labelling Methods

    doi: 10.2174/1874091X01408010011

    Figure Lengend Snippet: Sensitivity comparison between the FAST ® Frame Cassette and the Hybrislip™. ( A ) Different amounts (pmoles) of the same target, generated by the modified PCR method are plotted against the mean signal-background (RFU) for hybridization carried out in the FAST ® Frame hybridization chamber. ( B ) Different amounts (pmoles) of the same target, generated by the modified PCR method, are plotted against the mean signal-background (RFU) for hybridization carried out using the Hybrislip™.

    Article Snippet: Arrays were hybridized overnight in a humidified chamber at 53°C using either the FAST® Frame Cassette (Sigma, UK) or the Hybrislip™ (Grace Bio Labs, UK).

    Techniques: Generated, Modification, Polymerase Chain Reaction, Hybridization