Article Title: Flow Cessation triggers Endothelial Dysfunction during Organ Cold Storage Conditions: Strategies for Pharmacologic Intervention
Figure Lengend Snippet: Simvastatin inhibits endothelial activation after cold storage Top , EC monolayers were cultured for 24h under vasoprotective flow, followed by the immediate addition of IL-1β 0.1 U/mL, 4h, 37°C, left), or by cold storage for 6h with simvastatin (1 μM, right) or its vehicle (center), and then treated for 4h with IL-1β (as above). All groups were then incubated with fluorescently labeled HL-60 cells. Shown are representative fields of EC monolayers (gray) and attached HL60 cells (yellow). Bottom , quantitative fluorescence analysis of bound HL-60 cells (*p
Article Snippet: After 24h of vasoprotective flow stimulation, EC were washed twice with phosphate buffered saline (PBS) and incubated under static conditions for different periods of time at 37°C in complete EC culture media or at 4°C in UWS supplemented with Simvastatin (Calbiochem) or its vehicle DMSO (0.1% v/v).
Techniques: Activation Assay, Cell Culture, Flow Cytometry, Incubation, Labeling, Fluorescence