Journal: Nature Communications
Article Title: Nanoscale metal-organic frameworks for mitochondria-targeted radiotherapy-radiodynamic therapy
Figure Lengend Snippet: In vitro mechanistic studies of mitochondria-targeted RDT. a Fluorescence images of MC38 cells stained with JC-1 4 h after treatment of PBS, Hf-DBA, H 2 DBB-Ru and Hf-DBB-Ru with (2 Gy, + ) or without (-) X-ray irradiation. Green fluorescence indicates the depolarization of mitochondrial membrane potential. Scale bar = 50 µm. b Flow cytometric analysis of green versus red fluorescence of JC-1-stained MC38 cells 4 h after treatment of PBS and Hf-DBB-Ru with (2 Gy, + ) or without (-) X-ray irradiation. c cytochrome c released from mitochondria 8 h after treatment of PBS (left) or Hf-DBB-Ru (right) upon X-ray irradiation (2 Gy). Green: FITC-conjugated cytochrome c antibody; Red: Mitotracker CMXRos; Blue: DAPI. Scale bar = 10 µm. d Bcl-2 and Caspase-3 protein expression levels of MC38 cells 8 h after treatment of PBS, Hf-DBA, H 2 DBB-Ru and Hf-DBB-Ru upon X-ray irradiation (2 Gy). The glyceraldehyde 3-phosphate dehydrogenase (GAPDH) band served as loading control. The flow cytometry and CLSM studies were obtained with two repetitions to afford similar results
Article Snippet: After membrane transfer, anti-caspase-3 (#9662, Cell Signaling, USA, 1: 2000) and anti-Bcl-2 (Bcl-2–100, Thermofisher, USA, 1: 1000) were separately incubated with the cropped membranes for blotting.
Techniques: In Vitro, Fluorescence, Staining, Irradiation, Flow Cytometry, Expressing, Cytometry, Confocal Laser Scanning Microscopy