caspase 3 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Cell Signaling Technology Inc cleaved caspase 3
    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved <t>Caspase</t> 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P
    Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 3/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cleaved caspase 3 - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc caspase 3
    Evaluation of V-9302  in vivo  in HCC1806 cell line xenograft-bearing mice ( A ) Tumor volumetric analysis of mice treated with vehicle or V-9302 (75 mg/kg per day) for 10 days.  n  = 5 mice per group. Treatment started 9 days post injection. P value at day 10 determined by Student’s  t  test. ( B ) Immunofluorescence analysis of tumor tissues harvested from vehicle- or V-9302-treated mice; LC3B and pAKT (Ser473) shown (pink). CD-31 positive vessels shown in green and nuclei in blue (DAPI). 20× magnification. P values determined by Student’s  t  test. ( C ) Effects of V-9302 treatment on pS6-positive cells and caspase 3-positive cells by immunohistochemistry. Quantitative analysis consisted of the mean counts of at least three representative fields from three vehicle and three V-9302-treated mice. For box plots, center line is plotted at the median; the box spans from the first quartile to the third quartile; whiskers represent min to max. Error bars represent ± std. dev.
    Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    caspase 3 - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology caspase 3
    Frequencies of CD4 + and CD8 + T lymphocytes expressing caspase-1 or <t>caspase-3.</t> The frequency of CD4 + T lymphocyte with an expression of caspase-1 (a) and caspase-3 (b). The frequency of CD8 + T lymphocyte with an expression of caspase-1 (c) and caspase-3 (d). P Values are labelled in the figure for each comparison analysis. NC: non-infection control; EL: early latent stage; P1: phase 1; P2: phase 2.
    Caspase 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    caspase 3 - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc anti caspase 3
    In vitro mechanistic studies of mitochondria-targeted RDT. a Fluorescence images of MC38 cells stained with JC-1 4 h after treatment of PBS, Hf-DBA, H 2 DBB-Ru and Hf-DBB-Ru with (2 Gy, + ) or without (-) X-ray irradiation. Green fluorescence indicates the depolarization of mitochondrial membrane potential. Scale bar = 50 µm. b Flow cytometric analysis of green versus red fluorescence of JC-1-stained MC38 cells 4 h after treatment of PBS and Hf-DBB-Ru with (2 Gy, + ) or without (-) X-ray irradiation. c cytochrome c released from mitochondria 8 h after treatment of PBS (left) or Hf-DBB-Ru (right) upon X-ray irradiation (2 Gy). Green: FITC-conjugated cytochrome c antibody; Red: Mitotracker CMXRos; Blue: DAPI. Scale bar = 10 µm. d Bcl-2 and <t>Caspase-3</t> protein expression levels of MC38 cells 8 h after treatment of PBS, Hf-DBA, H 2 DBB-Ru and Hf-DBB-Ru upon X-ray irradiation (2 Gy). The glyceraldehyde 3-phosphate dehydrogenase (GAPDH) band served as loading control. The flow cytometry and CLSM studies were obtained with two repetitions to afford similar results
    Anti Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti caspase 3/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti caspase 3 - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    N/A
    Synonyms CPP 32 Apopain Yama SCA 1 Capase3 Price 325 00 Host rabbit Immunogen Synthetic peptide corresponding to AA 170 to 175 from mouse Caspase3 UniProt Id P70677 Reactivity human
      Buy from Supplier



    N/A
    Mouse monoclonal Caspase 3 antibody
      Buy from Supplier

    Image Search Results


    REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P

    Journal: Nature

    Article Title: Pharmacological activation of REV-ERBs is lethal in cancer and oncogene induced senescence

    doi: 10.1038/nature25170

    Figure Lengend Snippet: REV-ERB agonist SR9011 inhibit autophagy a–b, SR90011 treatment reduces the number of autophagosomes both in MCF7 and T47D, n= biological independent samples MCF7 20µM 24h n=9 (mock), n=4 (SR9011) ** P =0.0056, T47D 48h 20µM n=5 (mock), n=4 (SR9011) ** P =0.0079; c–d, SR9011 induces accumulation of p62 as shown by immunofluorescence both in MCF7 and T47D n= biological independent samples 48h MCF7 p62 n=3 (mock), n=4 (SR9011) * P =0.0286; 48h T47D n=5 (mock, SR9011) ** P =0.004; e, Accumulation of p62 is confirmed by immunoblot (48h, 20µM A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay (n= biological independent samples n=4 mock 48h, n=5 SR9011 p62, n=3 48h SR9011; n=6 (mock, SR9011 72h p62), n=10 (mock 72h), n=8 (SR9011 72h) A375 20µM, Cl. Casp. 3 48h * P =0.0286; Cl. Casp. 3 72h **** P

    Article Snippet: Apoptosis was evaluated by immunostaining of cleaved caspase 3 (Cell signaling #9664 1:200) and by TUNEL assay using In Situ Cell Death Detection Kit, Fluoresceine or TMR red (Roche).

    Techniques: Immunofluorescence, Inhibition, TUNEL Assay

    SR9009 and SR9011 treatment evokes an apoptotic response and induces inhibition of autophagy in OIS cells a, Proliferation assay shows that REV-ERBs agonists impair viability of OIS cells (6 days, 20µM). b–c, Immunofluorescence assay for cleaved Caspase 3 and TUNEL assay shows apoptosis induction specifically in OIS (n=biological independent samples, n=7 mock, n=9 SR9009, n=14 SR9011, 72h, 20µM; one-way ANOVA, Cl. Casp 3 **** P

    Journal: Nature

    Article Title: Pharmacological activation of REV-ERBs is lethal in cancer and oncogene induced senescence

    doi: 10.1038/nature25170

    Figure Lengend Snippet: SR9009 and SR9011 treatment evokes an apoptotic response and induces inhibition of autophagy in OIS cells a, Proliferation assay shows that REV-ERBs agonists impair viability of OIS cells (6 days, 20µM). b–c, Immunofluorescence assay for cleaved Caspase 3 and TUNEL assay shows apoptosis induction specifically in OIS (n=biological independent samples, n=7 mock, n=9 SR9009, n=14 SR9011, 72h, 20µM; one-way ANOVA, Cl. Casp 3 **** P

    Article Snippet: Apoptosis was evaluated by immunostaining of cleaved caspase 3 (Cell signaling #9664 1:200) and by TUNEL assay using In Situ Cell Death Detection Kit, Fluoresceine or TMR red (Roche).

    Techniques: Inhibition, Proliferation Assay, Immunofluorescence, TUNEL Assay

    REV-ERBs agonist SR9009 inhibits autophagy a–b , SR9009 treatment reduces the number of autophagosomes, as shown by immunofluorescence of LC3B, (n=biological independent samples MCF7 (n=6 mock), (n=5 SR9009) and T47D (n=5 mock) (n=4 SR9009) Mann–Whitney test one-tailed MCF7 20µM 24h * P =0.0152, T47D 20µM 48h ** P =0.0079; c–d SR9009 induces accumulation of p62 as shown by immunofluorescence; n= biological independent samples MCF7 (n=3 mock), (n=8 SR9009) and T47D (n=5 mock), (n=4 SR9009) Mann–Whitney test one-tailed 48h MCF7 p62 ** P =0.0061; 48h T47D ** P =0.0079; e, Inhibition of autophagy is confirmed by the immunoblot for p62 (20µM 48h, A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay; n=biological independent samples, Mann–Whitney test one-tailed, A375 20µM Cl. Casp. 3 48h (n=3) * P =0.0179; Cl. Casp. 3 72h (n=7) **** P

    Journal: Nature

    Article Title: Pharmacological activation of REV-ERBs is lethal in cancer and oncogene induced senescence

    doi: 10.1038/nature25170

    Figure Lengend Snippet: REV-ERBs agonist SR9009 inhibits autophagy a–b , SR9009 treatment reduces the number of autophagosomes, as shown by immunofluorescence of LC3B, (n=biological independent samples MCF7 (n=6 mock), (n=5 SR9009) and T47D (n=5 mock) (n=4 SR9009) Mann–Whitney test one-tailed MCF7 20µM 24h * P =0.0152, T47D 20µM 48h ** P =0.0079; c–d SR9009 induces accumulation of p62 as shown by immunofluorescence; n= biological independent samples MCF7 (n=3 mock), (n=8 SR9009) and T47D (n=5 mock), (n=4 SR9009) Mann–Whitney test one-tailed 48h MCF7 p62 ** P =0.0061; 48h T47D ** P =0.0079; e, Inhibition of autophagy is confirmed by the immunoblot for p62 (20µM 48h, A375); f–g, Inhibition of autophagy precedes apoptosis induction as shown by immunofluorescence of p62, cleaved Caspase 3 and TUNEL assay; n=biological independent samples, Mann–Whitney test one-tailed, A375 20µM Cl. Casp. 3 48h (n=3) * P =0.0179; Cl. Casp. 3 72h (n=7) **** P

    Article Snippet: Apoptosis was evaluated by immunostaining of cleaved caspase 3 (Cell signaling #9664 1:200) and by TUNEL assay using In Situ Cell Death Detection Kit, Fluoresceine or TMR red (Roche).

    Techniques: Immunofluorescence, MANN-WHITNEY, One-tailed Test, Inhibition, TUNEL Assay

    Evaluation of V-9302  in vivo  in HCC1806 cell line xenograft-bearing mice ( A ) Tumor volumetric analysis of mice treated with vehicle or V-9302 (75 mg/kg per day) for 10 days.  n  = 5 mice per group. Treatment started 9 days post injection. P value at day 10 determined by Student’s  t  test. ( B ) Immunofluorescence analysis of tumor tissues harvested from vehicle- or V-9302-treated mice; LC3B and pAKT (Ser473) shown (pink). CD-31 positive vessels shown in green and nuclei in blue (DAPI). 20× magnification. P values determined by Student’s  t  test. ( C ) Effects of V-9302 treatment on pS6-positive cells and caspase 3-positive cells by immunohistochemistry. Quantitative analysis consisted of the mean counts of at least three representative fields from three vehicle and three V-9302-treated mice. For box plots, center line is plotted at the median; the box spans from the first quartile to the third quartile; whiskers represent min to max. Error bars represent ± std. dev.

    Journal: Nature medicine

    Article Title: Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models

    doi: 10.1038/nm.4464

    Figure Lengend Snippet: Evaluation of V-9302 in vivo in HCC1806 cell line xenograft-bearing mice ( A ) Tumor volumetric analysis of mice treated with vehicle or V-9302 (75 mg/kg per day) for 10 days. n = 5 mice per group. Treatment started 9 days post injection. P value at day 10 determined by Student’s t test. ( B ) Immunofluorescence analysis of tumor tissues harvested from vehicle- or V-9302-treated mice; LC3B and pAKT (Ser473) shown (pink). CD-31 positive vessels shown in green and nuclei in blue (DAPI). 20× magnification. P values determined by Student’s t test. ( C ) Effects of V-9302 treatment on pS6-positive cells and caspase 3-positive cells by immunohistochemistry. Quantitative analysis consisted of the mean counts of at least three representative fields from three vehicle and three V-9302-treated mice. For box plots, center line is plotted at the median; the box spans from the first quartile to the third quartile; whiskers represent min to max. Error bars represent ± std. dev.

    Article Snippet: Tissues were sectioned (5 µm thickness) and stained for pS6 (Cell Signaling, #4858) and caspase 3 (Cell Signaling #9661).

    Techniques: In Vivo, Mouse Assay, Injection, Immunofluorescence, Immunohistochemistry

    Evaluation of V-9302 in vivo ( A ) Pharmacodynamic [ 18 F]-4F-Gln PET imaging prior to and 4 h following a single administration of V-9302 (75 mg/kg) in HCC1806 cell line xenograft-bearing mice (arrows indicate xenograft tumor on right flank*). ( B ) Mean time activity curves (TACs) from tumor regions of interest ( n = 4 measurements per condition); data prior to and 4 hrs following V-9302 administration. ( C ) P values determined by Student’s t test. Quantified tracer accumulation in xenograft tumors, muscle, and liver ( n = 4 measurements per condition). Volumetric analysis over 21 day treatment regimen (Vehicle or V-9302; 75 mg/kg, daily) of HCT-116 ( D ) and HT29 ( F ) cell line xenografts propagated in athymic nude mice ( n = 10 mice per group). Treatment started 12 days post tumor injection for HCT-116 and 4 days post injection for HT29. P values on day 21 determined by Student’s t test. Immunohistochemistry for pS6 and caspase 3 in vehicle-treated or V-9302-treated HCT-116 ( E ) and HT29 ( G ) xenografts. Representative photomicrographs and quantitation shown; magnification 20×. P values determined by Student’s t test. ( H ) Volumetric analysis over 31 day treatment regimen (Vehicle or V-9302; 75 mg/kg, daily) on athymic nude mice bearing patient-derived xenograft tumors (PDX A 008, F3 generation, treatment started 28 days post implantation, KRAS G12V ;p53 R248Q ;PTEN L140Y ; n = 10 mice per group) P value on day 31 determined by Student’s t test. ( I ) Photographs of A 008 PDX-bearing mice treated with V-9302 or vehicle; day 16 of 31. (Error bars represent ± std. dev. *Central photopenia observed.

    Journal: Nature medicine

    Article Title: Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models

    doi: 10.1038/nm.4464

    Figure Lengend Snippet: Evaluation of V-9302 in vivo ( A ) Pharmacodynamic [ 18 F]-4F-Gln PET imaging prior to and 4 h following a single administration of V-9302 (75 mg/kg) in HCC1806 cell line xenograft-bearing mice (arrows indicate xenograft tumor on right flank*). ( B ) Mean time activity curves (TACs) from tumor regions of interest ( n = 4 measurements per condition); data prior to and 4 hrs following V-9302 administration. ( C ) P values determined by Student’s t test. Quantified tracer accumulation in xenograft tumors, muscle, and liver ( n = 4 measurements per condition). Volumetric analysis over 21 day treatment regimen (Vehicle or V-9302; 75 mg/kg, daily) of HCT-116 ( D ) and HT29 ( F ) cell line xenografts propagated in athymic nude mice ( n = 10 mice per group). Treatment started 12 days post tumor injection for HCT-116 and 4 days post injection for HT29. P values on day 21 determined by Student’s t test. Immunohistochemistry for pS6 and caspase 3 in vehicle-treated or V-9302-treated HCT-116 ( E ) and HT29 ( G ) xenografts. Representative photomicrographs and quantitation shown; magnification 20×. P values determined by Student’s t test. ( H ) Volumetric analysis over 31 day treatment regimen (Vehicle or V-9302; 75 mg/kg, daily) on athymic nude mice bearing patient-derived xenograft tumors (PDX A 008, F3 generation, treatment started 28 days post implantation, KRAS G12V ;p53 R248Q ;PTEN L140Y ; n = 10 mice per group) P value on day 31 determined by Student’s t test. ( I ) Photographs of A 008 PDX-bearing mice treated with V-9302 or vehicle; day 16 of 31. (Error bars represent ± std. dev. *Central photopenia observed.

    Article Snippet: Tissues were sectioned (5 µm thickness) and stained for pS6 (Cell Signaling, #4858) and caspase 3 (Cell Signaling #9661).

    Techniques: In Vivo, Positron Emission Tomography, Imaging, Mouse Assay, Activity Assay, Injection, Immunohistochemistry, Quantitation Assay, Derivative Assay

    Frequencies of CD4 + and CD8 + T lymphocytes expressing caspase-1 or caspase-3. The frequency of CD4 + T lymphocyte with an expression of caspase-1 (a) and caspase-3 (b). The frequency of CD8 + T lymphocyte with an expression of caspase-1 (c) and caspase-3 (d). P Values are labelled in the figure for each comparison analysis. NC: non-infection control; EL: early latent stage; P1: phase 1; P2: phase 2.

    Journal: Innate Immunity

    Article Title: Syphilitic infection impairs immunity by inducing both apoptosis and pyroptosis of CD4+ and CD8+ T lymphocytes

    doi: 10.1177/1753425920952840

    Figure Lengend Snippet: Frequencies of CD4 + and CD8 + T lymphocytes expressing caspase-1 or caspase-3. The frequency of CD4 + T lymphocyte with an expression of caspase-1 (a) and caspase-3 (b). The frequency of CD8 + T lymphocyte with an expression of caspase-1 (c) and caspase-3 (d). P Values are labelled in the figure for each comparison analysis. NC: non-infection control; EL: early latent stage; P1: phase 1; P2: phase 2.

    Article Snippet: The percentage of CD4+ and CD8+ T cells expressing caspase-1 and caspase-3 increased in patients with syphilis To investigate the effect of syphilitic infection on the percentage of CD4+ and CD8+ T cells expressing caspase-1 and caspase-3, their levels of expression in the four study groups were analysed.

    Techniques: Expressing, Infection

    Correlation between serum levels and intracellular expression of caspase-1 and caspase-3 in CD4 + and CD8 + T cells. Correlations between caspase-1 and caspase-3 only take place at their serum levels and intracellular levels among (a) NC subjects, (b) patient with syphilis at EL stage, (c) patient with syphilis at P1 stage and (d) patient with syphilis at P2 stage. Blue and red colours represent a positive and negative correlation between the expression of caspase-1 and caspase-3 that meet at their serum and intracellular levels, respectively. The darker and more saturated the colour, the greater the magnitude of the correlation. Correlation matrices were displayed as schematic correlograms. 23 NC: non-infection control; EL: early latent stage; P1: phase 1; P2: phase 2.

    Journal: Innate Immunity

    Article Title: Syphilitic infection impairs immunity by inducing both apoptosis and pyroptosis of CD4+ and CD8+ T lymphocytes

    doi: 10.1177/1753425920952840

    Figure Lengend Snippet: Correlation between serum levels and intracellular expression of caspase-1 and caspase-3 in CD4 + and CD8 + T cells. Correlations between caspase-1 and caspase-3 only take place at their serum levels and intracellular levels among (a) NC subjects, (b) patient with syphilis at EL stage, (c) patient with syphilis at P1 stage and (d) patient with syphilis at P2 stage. Blue and red colours represent a positive and negative correlation between the expression of caspase-1 and caspase-3 that meet at their serum and intracellular levels, respectively. The darker and more saturated the colour, the greater the magnitude of the correlation. Correlation matrices were displayed as schematic correlograms. 23 NC: non-infection control; EL: early latent stage; P1: phase 1; P2: phase 2.

    Article Snippet: The percentage of CD4+ and CD8+ T cells expressing caspase-1 and caspase-3 increased in patients with syphilis To investigate the effect of syphilitic infection on the percentage of CD4+ and CD8+ T cells expressing caspase-1 and caspase-3, their levels of expression in the four study groups were analysed.

    Techniques: Expressing, Infection

    Levels of circulating caspase-1 and caspase-3 in the blood. Serum levels of caspase-1 (a) and caspase-3 (b) in healthy control subjects and patients with syphilis at stages of EL, P1 and P2. Correlation analysis of serum levels of caspase-1 and caspase-3. P Values are labelled in the figure for each comparison analysis. NC: non-infection control; EL: early latent stage; P1: phase 1; P2: phase 2.

    Journal: Innate Immunity

    Article Title: Syphilitic infection impairs immunity by inducing both apoptosis and pyroptosis of CD4+ and CD8+ T lymphocytes

    doi: 10.1177/1753425920952840

    Figure Lengend Snippet: Levels of circulating caspase-1 and caspase-3 in the blood. Serum levels of caspase-1 (a) and caspase-3 (b) in healthy control subjects and patients with syphilis at stages of EL, P1 and P2. Correlation analysis of serum levels of caspase-1 and caspase-3. P Values are labelled in the figure for each comparison analysis. NC: non-infection control; EL: early latent stage; P1: phase 1; P2: phase 2.

    Article Snippet: The percentage of CD4+ and CD8+ T cells expressing caspase-1 and caspase-3 increased in patients with syphilis To investigate the effect of syphilitic infection on the percentage of CD4+ and CD8+ T cells expressing caspase-1 and caspase-3, their levels of expression in the four study groups were analysed.

    Techniques: Infection

    In vitro mechanistic studies of mitochondria-targeted RDT. a Fluorescence images of MC38 cells stained with JC-1 4 h after treatment of PBS, Hf-DBA, H 2 DBB-Ru and Hf-DBB-Ru with (2 Gy, + ) or without (-) X-ray irradiation. Green fluorescence indicates the depolarization of mitochondrial membrane potential. Scale bar = 50 µm. b Flow cytometric analysis of green versus red fluorescence of JC-1-stained MC38 cells 4 h after treatment of PBS and Hf-DBB-Ru with (2 Gy, + ) or without (-) X-ray irradiation. c cytochrome c released from mitochondria 8 h after treatment of PBS (left) or Hf-DBB-Ru (right) upon X-ray irradiation (2 Gy). Green: FITC-conjugated cytochrome c antibody; Red: Mitotracker CMXRos; Blue: DAPI. Scale bar = 10 µm. d Bcl-2 and Caspase-3 protein expression levels of MC38 cells 8 h after treatment of PBS, Hf-DBA, H 2 DBB-Ru and Hf-DBB-Ru upon X-ray irradiation (2 Gy). The glyceraldehyde 3-phosphate dehydrogenase (GAPDH) band served as loading control. The flow cytometry and CLSM studies were obtained with two repetitions to afford similar results

    Journal: Nature Communications

    Article Title: Nanoscale metal-organic frameworks for mitochondria-targeted radiotherapy-radiodynamic therapy

    doi: 10.1038/s41467-018-06655-7

    Figure Lengend Snippet: In vitro mechanistic studies of mitochondria-targeted RDT. a Fluorescence images of MC38 cells stained with JC-1 4 h after treatment of PBS, Hf-DBA, H 2 DBB-Ru and Hf-DBB-Ru with (2 Gy, + ) or without (-) X-ray irradiation. Green fluorescence indicates the depolarization of mitochondrial membrane potential. Scale bar = 50 µm. b Flow cytometric analysis of green versus red fluorescence of JC-1-stained MC38 cells 4 h after treatment of PBS and Hf-DBB-Ru with (2 Gy, + ) or without (-) X-ray irradiation. c cytochrome c released from mitochondria 8 h after treatment of PBS (left) or Hf-DBB-Ru (right) upon X-ray irradiation (2 Gy). Green: FITC-conjugated cytochrome c antibody; Red: Mitotracker CMXRos; Blue: DAPI. Scale bar = 10 µm. d Bcl-2 and Caspase-3 protein expression levels of MC38 cells 8 h after treatment of PBS, Hf-DBA, H 2 DBB-Ru and Hf-DBB-Ru upon X-ray irradiation (2 Gy). The glyceraldehyde 3-phosphate dehydrogenase (GAPDH) band served as loading control. The flow cytometry and CLSM studies were obtained with two repetitions to afford similar results

    Article Snippet: After membrane transfer, anti-caspase-3 (#9662, Cell Signaling, USA, 1: 2000) and anti-Bcl-2 (Bcl-2–100, Thermofisher, USA, 1: 1000) were separately incubated with the cropped membranes for blotting.

    Techniques: In Vitro, Fluorescence, Staining, Irradiation, Flow Cytometry, Expressing, Cytometry, Confocal Laser Scanning Microscopy