cas9 protein Search Results


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  • 96
    Cell Signaling Technology Inc cleaved caspase 9
    Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cleaved caspase 9/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cleaved caspase 9 - by Bioz Stars, 2021-06
    96/100 stars
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    99
    Integrated DNA Technologies cas9 protein
    HSPC-pDCs are amenable to genetic modifications. a Graphical illustration of the protocol for <t>CRISPR/Cas9-mediated</t> gene editing using Cas9 RNP. CD34 + HSPCs were initially cultured in CD34 + HSPC medium at low density for 3 days before being electroporated with Cas9 RNP complexes. After a 3-day recovery phase, pDC differentiation was initiated. b Expansion of cells during differentiation from HSPCs into HSPC-pDCs. c Indel frequencies after the 3-day recovery phase quantified by TIDE analysis for cells targeted at MyD88 , IFNAR1 , and CCR5 (control). d Numbers of HSPC-pDCs at day 21 for gene-edited HSPC-pDCs. e Western blot analysis showing induction of pSTAT1 upon 3 h IFN-β stimulation (upper panel) and protein levels of MyD88 (lower panel) in HSPC-pDCs targeted at IFNAR1 and MyD88 , respectively (detection Ab for STAT1: CST 9172). Indel frequencies at the respective targets are listed below the western blot. f , g Surface expression levels (MFI) of CD123 ( f ) and CD304 ( g ) in IFN-primed and unprimed HSPC-pDCs with gene editing at MyD88 , IFNAR1, and CCR5 . h Functional levels of type I IFN after stimulation with R837 (TLR7) or CpG-A (TLR9) in unprimed and IFN-primed HSPC-pDCs gene-edited at CCR5 , MyD88 , or CCR5 . i Levels of IL-6 after stimulation with R837 (TLR7) or CpG-A (TLR9) in unprimed and IFN-primed HSPC-pDCs targeted at CCR5, MyD88, or IFNAR1 . Data are ±SEM of five ( b – d ) or three ( f – i
    Cas9 Protein, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cas9 protein/product/Integrated DNA Technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cas9 protein - by Bioz Stars, 2021-06
    99/100 stars
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    86
    New England Biolabs cas9 enzyme
    Evaluation of <t>Cas9</t> injection controls. ( A) DE genes across samples injected with Cas9 enzyme or Cas9 mRNA relative to uninjected batch-siblings. Plot includes the number and percentage of genes downregulated (FoldChange
    Cas9 Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cas9 enzyme/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cas9 enzyme - by Bioz Stars, 2021-06
    86/100 stars
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    99
    Cell Signaling Technology Inc anti cleaved caspase 9
    Evaluation of <t>Cas9</t> injection controls. ( A) DE genes across samples injected with Cas9 enzyme or Cas9 mRNA relative to uninjected batch-siblings. Plot includes the number and percentage of genes downregulated (FoldChange
    Anti Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase 9/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cleaved caspase 9 - by Bioz Stars, 2021-06
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    N/A
    Purified recombinant protein of mutant D10A of S pyogenes Cas9 endonuclease Cas9 containing Simian virus 40 SV40 T antigen nuclear localization sequence NLS on the N and C termini of
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    N/A
    CRISPR Associated Protein 9 Nuclease S Pyogenes Recombinant 100 ug
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    Image Search Results


    HSPC-pDCs are amenable to genetic modifications. a Graphical illustration of the protocol for CRISPR/Cas9-mediated gene editing using Cas9 RNP. CD34 + HSPCs were initially cultured in CD34 + HSPC medium at low density for 3 days before being electroporated with Cas9 RNP complexes. After a 3-day recovery phase, pDC differentiation was initiated. b Expansion of cells during differentiation from HSPCs into HSPC-pDCs. c Indel frequencies after the 3-day recovery phase quantified by TIDE analysis for cells targeted at MyD88 , IFNAR1 , and CCR5 (control). d Numbers of HSPC-pDCs at day 21 for gene-edited HSPC-pDCs. e Western blot analysis showing induction of pSTAT1 upon 3 h IFN-β stimulation (upper panel) and protein levels of MyD88 (lower panel) in HSPC-pDCs targeted at IFNAR1 and MyD88 , respectively (detection Ab for STAT1: CST 9172). Indel frequencies at the respective targets are listed below the western blot. f , g Surface expression levels (MFI) of CD123 ( f ) and CD304 ( g ) in IFN-primed and unprimed HSPC-pDCs with gene editing at MyD88 , IFNAR1, and CCR5 . h Functional levels of type I IFN after stimulation with R837 (TLR7) or CpG-A (TLR9) in unprimed and IFN-primed HSPC-pDCs gene-edited at CCR5 , MyD88 , or CCR5 . i Levels of IL-6 after stimulation with R837 (TLR7) or CpG-A (TLR9) in unprimed and IFN-primed HSPC-pDCs targeted at CCR5, MyD88, or IFNAR1 . Data are ±SEM of five ( b – d ) or three ( f – i

    Journal: Nature Communications

    Article Title: Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function

    doi: 10.1038/s41467-018-05816-y

    Figure Lengend Snippet: HSPC-pDCs are amenable to genetic modifications. a Graphical illustration of the protocol for CRISPR/Cas9-mediated gene editing using Cas9 RNP. CD34 + HSPCs were initially cultured in CD34 + HSPC medium at low density for 3 days before being electroporated with Cas9 RNP complexes. After a 3-day recovery phase, pDC differentiation was initiated. b Expansion of cells during differentiation from HSPCs into HSPC-pDCs. c Indel frequencies after the 3-day recovery phase quantified by TIDE analysis for cells targeted at MyD88 , IFNAR1 , and CCR5 (control). d Numbers of HSPC-pDCs at day 21 for gene-edited HSPC-pDCs. e Western blot analysis showing induction of pSTAT1 upon 3 h IFN-β stimulation (upper panel) and protein levels of MyD88 (lower panel) in HSPC-pDCs targeted at IFNAR1 and MyD88 , respectively (detection Ab for STAT1: CST 9172). Indel frequencies at the respective targets are listed below the western blot. f , g Surface expression levels (MFI) of CD123 ( f ) and CD304 ( g ) in IFN-primed and unprimed HSPC-pDCs with gene editing at MyD88 , IFNAR1, and CCR5 . h Functional levels of type I IFN after stimulation with R837 (TLR7) or CpG-A (TLR9) in unprimed and IFN-primed HSPC-pDCs gene-edited at CCR5 , MyD88 , or CCR5 . i Levels of IL-6 after stimulation with R837 (TLR7) or CpG-A (TLR9) in unprimed and IFN-primed HSPC-pDCs targeted at CCR5, MyD88, or IFNAR1 . Data are ±SEM of five ( b – d ) or three ( f – i

    Article Snippet: Ribonucleoprotein (RNP) complexes were made by incubating Cas9 protein (Integrated DNA Technologies) with sgRNA at a molar ratio of 1:2.5 at 25 °C for 10 min prior to nucleoporation.

    Techniques: CRISPR, Cell Culture, Western Blot, Expressing, Functional Assay

    Evaluation of Cas9 injection controls. ( A) DE genes across samples injected with Cas9 enzyme or Cas9 mRNA relative to uninjected batch-siblings. Plot includes the number and percentage of genes downregulated (FoldChange

    Journal: bioRxiv

    Article Title: Evaluation of CRISPR gene-editing tools in zebrafish identifies spurious mutations in ‘mock’ control embryos

    doi: 10.1101/2020.10.19.345256

    Figure Lengend Snippet: Evaluation of Cas9 injection controls. ( A) DE genes across samples injected with Cas9 enzyme or Cas9 mRNA relative to uninjected batch-siblings. Plot includes the number and percentage of genes downregulated (FoldChange

    Article Snippet: Injection mix for Cas9 enzyme included Cas9 enzyme (20 μM, New England BioLabs), 2.5 μl of 4x Injection Buffer (0.2% phenol red, 800 mM KCl, 4 mM MgCl2 , 4 mM TCEP, 120 mM HEPES, pH 7.0), and Nuclease-free water.

    Techniques: Injection