Journal: Frontiers in Immunology
Article Title: Marijuana-Derived Cannabinoids Trigger a CB2/PI3K Axis of Suppression of the Innate Response to Oral Pathogens
Figure Lengend Snippet: CBD suppresses inflammation induced by repeated oral inoculation with P. gingivalis . CB2 −/− and control groups of C57Bl6 mice ( n = 5 per group) were treated with cannabidiol or vehicle control and repeatedly orally infected with P. gingivalis 33277 or carboxymethyl cellulose control. Total serum IgM was measured by ELISA (A,B) ; and gingival tissue transcripts of inflammation-related biomarkers were detected by qPCR (C–H) . There were no differences in the total IgG or P. gingivalis -cognizant IgM or IgG titers of WT vs. CB2 −/− animals ( data not shown ). Total IgM, IL-1β, CD14, and CD45 signals differed in WT mice treated with P. gingivalis only compared to those dually treated with bacteria and CBD in WT animals only, all p
Article Snippet: Ultrapure LPS from Escherichia coli 0111:B, trypan blue, the serine and cysteine protease/gingipain inhibitor tosyl-L-lysine chloromethylketone, gelatin, volatile fatty acids (glacial acetic, propionic, N-butyric, N-valeric, isobutyric, isovaleric, and DL-methylbutyric acids), carboxymethyl cellulose salt, methanol, isoflurane, RNAlater, agarose, methylene blue, eosin, hematoxylin, L-cysteine, and arginine were bought from Sigma-Aldrich (St. Louis, MO).
Techniques: Mouse Assay, Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction