Journal:

Article Title: Proficiency Testing Program for Clinical Laboratories Performing Antifungal Susceptibility Testing of Pathogenic Yeast Species

doi: 10.1128/JCM.41.3.1143-1146.2003

Figure Lengend Snippet: Methods for antifungal susceptibility testing for amphotericin B and comparison of results with the NCCLS reference range

Article Snippet: For C . lusitaniae ATCC 200950, the MIC range from an earlier publication was used ( 1 , 10 , 19 ).

Techniques:

Journal:

Article Title: Proficiency Testing Program for Clinical Laboratories Performing Antifungal Susceptibility Testing of Pathogenic Yeast Species

doi: 10.1128/JCM.41.3.1143-1146.2003

Figure Lengend Snippet: Methods for antifungal susceptibility testing for fluconazole and comparison of results with the NCCLS reference range

Article Snippet: For C . lusitaniae ATCC 200950, the MIC range from an earlier publication was used ( 1 , 10 , 19 ).

Techniques:

Journal:

Article Title: Meiotic Chromosome Synapsis in Yeast Can Occur Without Spo11-Induced DNA Double-Strand Breaks

doi: 10.1534/genetics.104.029660

Figure Lengend Snippet: Percentage of cells with different Zip1-staining patterns (classes A–D) at pachytene (5 hr in sporulation medium)

Article Snippet: A summary of the chromosome pairing data is given in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Background labeling (%) Strain Probe chromosome position Harvesting time (hr) 1 dot (%) 2 dots (%) >2 dots 0 dots % pairing after subtracting background N spo11 Δ Subtelomeric 5 40 46 9 5 47 415 spo11 Δ ndt80 Δ Subtelomeric 5 45 40 8 7 53 224 spo11 Δ ndt80 Δ Subtelomeric 8 46 40 6 8 53 181 Wild type Subtelomeric 5 47 37 9 7 56 180 spo11 Δ Interstitial 5 23 64 4 9 26 287 spo11 Δ ndt80 Δ Interstitial 5 28 57 7 9 33 172 spo11 Δ ndt80 Δ Interstitial 8 30 59 3 7 34 174 Wild type Interstitial 5 48 30 12 10 62 129 Open in a separate window The percentage of mutant and wild-type cells undergoing homologous chromosome pairing and nonpairing in FISH experiments with subtelomeric (ATCC no. 70884) and interstitial (ATCC no. 71216) probes.

Techniques:

Journal:

Article Title: Meiotic Chromosome Synapsis in Yeast Can Occur Without Spo11-Induced DNA Double-Strand Breaks

doi: 10.1534/genetics.104.029660

Figure Lengend Snippet: SC structures based on Zip1 staining in spread preparations at pachytene. (a–c) Full complement of SCs in the spo11 mutant (NKY2967). (d–f) Full complement of SCs in the spo11ndt80 double mutant (NKY2535). (g–i) Full complement of SCs in wild-type (NKY1551) cells (g and h show average appearance of class A nuclei, while i is an example of an “exceptionally good-looking” nucleus). Corresponding DNA staining is shown in a′–i′. For information on frequency of different classes in the different strains, see Table 2. Bar, 4 μm.

Article Snippet: A summary of the chromosome pairing data is given in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Background labeling (%) Strain Probe chromosome position Harvesting time (hr) 1 dot (%) 2 dots (%) >2 dots 0 dots % pairing after subtracting background N spo11 Δ Subtelomeric 5 40 46 9 5 47 415 spo11 Δ ndt80 Δ Subtelomeric 5 45 40 8 7 53 224 spo11 Δ ndt80 Δ Subtelomeric 8 46 40 6 8 53 181 Wild type Subtelomeric 5 47 37 9 7 56 180 spo11 Δ Interstitial 5 23 64 4 9 26 287 spo11 Δ ndt80 Δ Interstitial 5 28 57 7 9 33 172 spo11 Δ ndt80 Δ Interstitial 8 30 59 3 7 34 174 Wild type Interstitial 5 48 30 12 10 62 129 Open in a separate window The percentage of mutant and wild-type cells undergoing homologous chromosome pairing and nonpairing in FISH experiments with subtelomeric (ATCC no. 70884) and interstitial (ATCC no. 71216) probes.

Techniques: Staining, Mutagenesis

Journal:

Article Title: Meiotic Chromosome Synapsis in Yeast Can Occur Without Spo11-Induced DNA Double-Strand Breaks

doi: 10.1534/genetics.104.029660

Figure Lengend Snippet: Percentage of cells with different Zip1-staining patterns (classes A–E) after different periods of time in sporulation medium

Article Snippet: A summary of the chromosome pairing data is given in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Background labeling (%) Strain Probe chromosome position Harvesting time (hr) 1 dot (%) 2 dots (%) >2 dots 0 dots % pairing after subtracting background N spo11 Δ Subtelomeric 5 40 46 9 5 47 415 spo11 Δ ndt80 Δ Subtelomeric 5 45 40 8 7 53 224 spo11 Δ ndt80 Δ Subtelomeric 8 46 40 6 8 53 181 Wild type Subtelomeric 5 47 37 9 7 56 180 spo11 Δ Interstitial 5 23 64 4 9 26 287 spo11 Δ ndt80 Δ Interstitial 5 28 57 7 9 33 172 spo11 Δ ndt80 Δ Interstitial 8 30 59 3 7 34 174 Wild type Interstitial 5 48 30 12 10 62 129 Open in a separate window The percentage of mutant and wild-type cells undergoing homologous chromosome pairing and nonpairing in FISH experiments with subtelomeric (ATCC no. 70884) and interstitial (ATCC no. 71216) probes.

Techniques:

Journal:

Article Title: Meiotic Chromosome Synapsis in Yeast Can Occur Without Spo11-Induced DNA Double-Strand Breaks

doi: 10.1534/genetics.104.029660

Figure Lengend Snippet: Pairing of chromosomes investigated by hybridization with subtelomeric vs. interstitial probes

Article Snippet: A summary of the chromosome pairing data is given in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Background labeling (%) Strain Probe chromosome position Harvesting time (hr) 1 dot (%) 2 dots (%) >2 dots 0 dots % pairing after subtracting background N spo11 Δ Subtelomeric 5 40 46 9 5 47 415 spo11 Δ ndt80 Δ Subtelomeric 5 45 40 8 7 53 224 spo11 Δ ndt80 Δ Subtelomeric 8 46 40 6 8 53 181 Wild type Subtelomeric 5 47 37 9 7 56 180 spo11 Δ Interstitial 5 23 64 4 9 26 287 spo11 Δ ndt80 Δ Interstitial 5 28 57 7 9 33 172 spo11 Δ ndt80 Δ Interstitial 8 30 59 3 7 34 174 Wild type Interstitial 5 48 30 12 10 62 129 Open in a separate window The percentage of mutant and wild-type cells undergoing homologous chromosome pairing and nonpairing in FISH experiments with subtelomeric (ATCC no. 70884) and interstitial (ATCC no. 71216) probes.

Techniques: Hybridization, Labeling

Journal:

Article Title: Meiotic Chromosome Synapsis in Yeast Can Occur Without Spo11-Induced DNA Double-Strand Breaks

doi: 10.1534/genetics.104.029660

Figure Lengend Snippet: Cells with complete SCs (class A) in Δ spo11/ Δ ndt80 and wild type grown under different physical conditions and sporulation frequency

Article Snippet: A summary of the chromosome pairing data is given in . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Background labeling (%) Strain Probe chromosome position Harvesting time (hr) 1 dot (%) 2 dots (%) >2 dots 0 dots % pairing after subtracting background N spo11 Δ Subtelomeric 5 40 46 9 5 47 415 spo11 Δ ndt80 Δ Subtelomeric 5 45 40 8 7 53 224 spo11 Δ ndt80 Δ Subtelomeric 8 46 40 6 8 53 181 Wild type Subtelomeric 5 47 37 9 7 56 180 spo11 Δ Interstitial 5 23 64 4 9 26 287 spo11 Δ ndt80 Δ Interstitial 5 28 57 7 9 33 172 spo11 Δ ndt80 Δ Interstitial 8 30 59 3 7 34 174 Wild type Interstitial 5 48 30 12 10 62 129 Open in a separate window The percentage of mutant and wild-type cells undergoing homologous chromosome pairing and nonpairing in FISH experiments with subtelomeric (ATCC no. 70884) and interstitial (ATCC no. 71216) probes.

Techniques: Concentration Assay

Journal:

Article Title: Detection of Resistance to Amphotericin B in Candida Isolates by Using Iso-Sensitest Broth

doi: 10.1128/AAC.45.7.2070-2074.2001

Figure Lengend Snippet: AMB MICs determined spectrophotometrically a with Iso-Sensitest–2% glucose as culture medium

Article Snippet: The MICs for susceptible isolates fell when AST was performed with Iso-Sensitest supplemented with glucose, and these methods showed enhanced abilities to discriminate between putatively susceptible and resistant isolates (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Isolate MIC (μg/ml) 10 3 -CFU/ml inoculum 10 5 -CFU/ml inoculum 24-h incubation 48-h incubation 24-h incubation 48-h incubation Susceptible isolates C. albicans CNM-CL-3414 0.007 0.03 0.015 0.06 C. albicans CNM-CL-3416 0.007 0.06 0.06 0.25 C. albicans CNM-CL-3419 0.004 0.015 0.015 0.03 C. albicans CNM-CL-3420 0.007 0.06 0.03 0.12 C. parapsilosis ATCC 200954 0.007 0.03 0.015 0.03 C. parapsilosis CNM-CL-3413 0.004 0.015 0.007 0.06 C. parapsilosis CNM-CL-3417 0.004 0.015 0.007 0.06 C. lusitaniae CNM-CL-3418 0.007 0.03 0.03 0.12 Resistant isolates C. lusitaniae ATCC 200950 0.25 0.50 0.25 0.50 C. lusitaniae ATCC 200951 0.12 0.25 0.25 0.50 C. lusitaniae ATCC 200952 0.12 0.50 0.12 0.50 C. lusitaniae ATCC 200953 0.03 0.12 0.12 0.50 C. albicans ATCC 200955 0.12 1.00 0.50 2.00 C. tropicalis ATCC 200956 2.00 8.00 8.00 8.00 Open in a separate window a Optical density at 540 nm.

Techniques: Incubation