Journal: Scientific Reports
Article Title: Rare Helicobacter pylori Virulence Genotypes in Bhutan
Figure Lengend Snippet: Variation in the CagA amino acid sequence of East Asian-type and Western-type CagA. The target α-EAS sequences “AINRKIDRINKIASAGKG” in EPIYA segment D, based on the sequences of Japanese strains, are shown in frame. A sequence analysis revealed that the typical target sequences in Bhutanese strains was “(T/K)IN(G/R)KID(Q/R)(L/I)N(R/K)(T/I)ASA(G/N)KG,” where (X/Y) means X and Y as the two major amino acids. The sequences of the EPIYA-D segments in Bhutanese strains were highly variable compared to strains deposited in GenBank. In contrast, the East Asian-type and Western-type sequences of EPIYA-C segments in Bhutanese strains were largely identical, similar to the typical sequences of EPIYA-C segments deposited in GenBank. Reference strains used in Fig. 1A (strain name [accession number]) were 103a (AB110966.1), 105a (AB110967.1), 106a (AB110968.1), 108a (AB110969.1), 113b (AB110970.1), 120a (AB110971.1), 122b (AB110972.1), 125b (AB110973.1), 128a (AB110974.1), FJT77 (KF028580.1), 04-518 (AB267252.1), 03-166 (AB267253.1), 04-264 (AB267254.1), THP1477 (AB116744.1), 04-334 (AB267249.1), 03-292 (AB267250.1), 04-366 (AB267251.1), THP1260 (AB116742.1), M3 (AB116740.1), THP463 (AB116735.1), Korea23 (AB057044.1), Korea 12 (AB057043.1), K69 (FJ458129.1), Korea2-3 (AB057040.1), k266 (FJ458163.1), K265 (FJ458162.1), K264 (FJ458161.1), K261 (FJ458158.1), K260 (FJ458157.1), K259 (FJ458156.1). Reference strains used in Fig. 1C (strain name [accession number]) were India41 (AF222807.1), India99 (AF222809.1), OSC40A (EU089774.1), OSC42B (EU089775.1), PCR-156i (EU368669.1), PCR218vi (EU089766.1). RIGLD-OC149 (JX428784.1), SAN53 (EU089771.1), PD682 (EF450167.1), PD636 (EF450165.1), PD308 (EF450162.1), PD488 (EF450161.1), PD537 (EF450160.1), PD501 (EF450159.1), PD351 (EF450158.1), PD348 (EF450157.1), PD6481K (EF450153.1), 216G (GQ899171.1), 1407 (GU143415.1), HPI-14 (FJ849792.1), HPI-13 (FJ849791.1), HPI-11 (FJ849789.1), USA2791 (AB057099.1), Kazak3 (AB057098.1), USA35 (AB057095.1), Italy329 (AB057094.1), Arizona2 (AB057075.1), Arizona1 (AB057074.1).
Article Snippet: Briefly, after antigen retrieval and inactivation of endogenous peroxidase activity, tissue sections were incubated with α-H. pylori antibody (DAKO, Glostrup, Denmark), anti-CagA antibody (b-300; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or α-EAS Ab diluted 1:2,000 with diluting solution (DAKO) overnight at 4 °C.
Techniques: Sequencing, Western Blot, Polymerase Chain Reaction