cadherin 11 Search Results


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    Santa Cruz Biotechnology ob cadherin
    (A) Bright field images of mesensphere formation and at days 1, 2, 7 and 14. (B) Western Blot of <t>N-cadherin</t> siRNA (80 or 160 nM concentrations), scrambled siRNA at 80 nM concentration and untreated, control MSCs. (C) Western Blot of OB-cadherin siRNA (80 concentration), scrambled siRNA at 80 nM concentration and untreated, control MSCs. Groups: Cont: untreated control, Scram: scrambled siRNA, —N: N-cadherin siRNA, —OB: OB-cadherin siRNA.
    Ob Cadherin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ob cadherin/product/Santa Cruz Biotechnology
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    94
    Thermo Fisher cadherin 11
    The distribution of <t>cadherin</t> 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.
    Cadherin 11, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    93
    Abcam ob cadherin
    Effect of <t>E-cadherin</t> knock-down on the expression of SNAI1 and other transcriptional factors. (A) Sh-PC3 and ShEC-PC3 cells were collected at similar confluency and nuclear/cytoplasmic fractions were prepared and analyzed for SNAI1, β-catenin, and p65 expression by Western blotting. Tubulin and histone H1 were used as loading control for cytoplasmic and nuclear fractions respectively. (B) Sh-PC3 and ShEC-PC3 prostaspheres were collected following centrifugation and cell lysates were prepared and analyzed for SNAI1 expression by Western blotting. (C) Sh-PC3 and ShEC-PC3 xenograft tissues were analyzed for the expression of SNAI1 by IHC. Immunoreactivity score was analyzed in 5 random areas for each tumor tissue and was scored as 0+ (no staining), 1+ (weak staining), 2+ (moderate staining), 3+ (strong staining), 4+ (very strong staining). Percentage of SNAI1 positive cells was calculated by counting the number of positive stained cells (brown stained) and the total number of cells at five arbitrarily selected fields from each tumor at 400x magnification. The data shown in the bar diagrams is the mean±SEM of 7–10 samples. *, p ≤ 0.001.
    Ob Cadherin, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher anti cadherin 11
    Cadherin-8 and <t>cadherin-11</t> are enriched in postsynaptic densities and cadherin-8 interacts with neuroligin-1. (A) Forebrain tissues were subjected to synaptic fractionation analysis to determine the subcellular localization of cadherin-8 and cadherin-11. Markers (PSD-95 and syntaxin-1) were probed as control for purity of the fractionation. Total: total protein input, P1: nuclear, S1: cytosol/membranes, P2: crude synaptosome, S2: cytosol/light membranes, P3: synaptosome, SPM: synaptic plasma membrane, PSD: postsynaptic density, S3: synaptic vesicles. (B) Quantification of protein enrichment in SPM and PSD fractions compared to total protein input. PSD-95: ** p = 0.0012 between SPM and total, **** p
    Anti Cadherin 11, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    16A recognizes the extracellular domain of cadherin 11 Isotype Note IgG1 Host Species Note Mouse Human|Rat
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    N/A
    Rabbit polyclonal antibody to OB cadherin Isotype Note IgG Host Note Rabbit Conjugation Note Unconjugated Reactivity Note Human Mouse Application Note ELISA WB
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    Image Search Results


    (A) Bright field images of mesensphere formation and at days 1, 2, 7 and 14. (B) Western Blot of N-cadherin siRNA (80 or 160 nM concentrations), scrambled siRNA at 80 nM concentration and untreated, control MSCs. (C) Western Blot of OB-cadherin siRNA (80 concentration), scrambled siRNA at 80 nM concentration and untreated, control MSCs. Groups: Cont: untreated control, Scram: scrambled siRNA, —N: N-cadherin siRNA, —OB: OB-cadherin siRNA.

    Journal: Journal of biomechanics

    Article Title: The role of adhesion junctions in the biomechanical behaviour and osteogenic differentiation of 3D mesenchymal stem cell spheroids

    doi: 10.1016/j.jbiomech.2017.05.014

    Figure Lengend Snippet: (A) Bright field images of mesensphere formation and at days 1, 2, 7 and 14. (B) Western Blot of N-cadherin siRNA (80 or 160 nM concentrations), scrambled siRNA at 80 nM concentration and untreated, control MSCs. (C) Western Blot of OB-cadherin siRNA (80 concentration), scrambled siRNA at 80 nM concentration and untreated, control MSCs. Groups: Cont: untreated control, Scram: scrambled siRNA, —N: N-cadherin siRNA, —OB: OB-cadherin siRNA.

    Article Snippet: The small interfering RNA (siRNA) oligonucleotides for N-cadherin (sc-35999), OB-cadherin (sc-36114) and a scrambled control (sc-37007) were obtained from Santa Cruz.

    Techniques: Western Blot, Concentration Assay

    BMP2 immunofluorescent staining of mesenspheres at day 7 and day 14. Groups: Scram: scrambled siRNA, —OB: OB-cadherin siRNA, —N: N-cadherin siRNA. Scale bars represent 10 μm. The corrected total cell fluorescence per area surface was measured for each condition (B).

    Journal: Journal of biomechanics

    Article Title: The role of adhesion junctions in the biomechanical behaviour and osteogenic differentiation of 3D mesenchymal stem cell spheroids

    doi: 10.1016/j.jbiomech.2017.05.014

    Figure Lengend Snippet: BMP2 immunofluorescent staining of mesenspheres at day 7 and day 14. Groups: Scram: scrambled siRNA, —OB: OB-cadherin siRNA, —N: N-cadherin siRNA. Scale bars represent 10 μm. The corrected total cell fluorescence per area surface was measured for each condition (B).

    Article Snippet: The small interfering RNA (siRNA) oligonucleotides for N-cadherin (sc-35999), OB-cadherin (sc-36114) and a scrambled control (sc-37007) were obtained from Santa Cruz.

    Techniques: Staining, Fluorescence

    The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Genomic Locus Modulating IOP in the BXD RI Mouse Strains

    doi: 10.1534/g3.118.200190

    Figure Lengend Snippet: The distribution of cadherin 11 in the limbal area of the eye is illustrated. The section in A was stained with an antibody specific to cadherin 11 (green) and for DNA (blue). This staining is specific to the primary antibody for it is not observed in a section stained with the secondary antibody alone (B). The staining pattern of the trabecular meshwork is shown at higher magnification in C (arrow). A and B are taken at the same magnification and the scale bar in panel B represents 25 µm.

    Article Snippet: The sections were incubated in primary antibodies (1:500) against Cadherin 11 (Thermofisher, Cat. #71-7600, Waltham, MA) overnight at 4°.

    Techniques: Staining

    Effect of E-cadherin knock-down on the expression of SNAI1 and other transcriptional factors. (A) Sh-PC3 and ShEC-PC3 cells were collected at similar confluency and nuclear/cytoplasmic fractions were prepared and analyzed for SNAI1, β-catenin, and p65 expression by Western blotting. Tubulin and histone H1 were used as loading control for cytoplasmic and nuclear fractions respectively. (B) Sh-PC3 and ShEC-PC3 prostaspheres were collected following centrifugation and cell lysates were prepared and analyzed for SNAI1 expression by Western blotting. (C) Sh-PC3 and ShEC-PC3 xenograft tissues were analyzed for the expression of SNAI1 by IHC. Immunoreactivity score was analyzed in 5 random areas for each tumor tissue and was scored as 0+ (no staining), 1+ (weak staining), 2+ (moderate staining), 3+ (strong staining), 4+ (very strong staining). Percentage of SNAI1 positive cells was calculated by counting the number of positive stained cells (brown stained) and the total number of cells at five arbitrarily selected fields from each tumor at 400x magnification. The data shown in the bar diagrams is the mean±SEM of 7–10 samples. *, p ≤ 0.001.

    Journal: Molecular Cancer

    Article Title: SNAI1 is critical for the aggressiveness of prostate cancer cells with low E-cadherin

    doi: 10.1186/1476-4598-13-37

    Figure Lengend Snippet: Effect of E-cadherin knock-down on the expression of SNAI1 and other transcriptional factors. (A) Sh-PC3 and ShEC-PC3 cells were collected at similar confluency and nuclear/cytoplasmic fractions were prepared and analyzed for SNAI1, β-catenin, and p65 expression by Western blotting. Tubulin and histone H1 were used as loading control for cytoplasmic and nuclear fractions respectively. (B) Sh-PC3 and ShEC-PC3 prostaspheres were collected following centrifugation and cell lysates were prepared and analyzed for SNAI1 expression by Western blotting. (C) Sh-PC3 and ShEC-PC3 xenograft tissues were analyzed for the expression of SNAI1 by IHC. Immunoreactivity score was analyzed in 5 random areas for each tumor tissue and was scored as 0+ (no staining), 1+ (weak staining), 2+ (moderate staining), 3+ (strong staining), 4+ (very strong staining). Percentage of SNAI1 positive cells was calculated by counting the number of positive stained cells (brown stained) and the total number of cells at five arbitrarily selected fields from each tumor at 400x magnification. The data shown in the bar diagrams is the mean±SEM of 7–10 samples. *, p ≤ 0.001.

    Article Snippet: SNAI1, N-cadherin, OB-cadherin and TATA-binding protein (TBP) antibodies were from Abcam (Cambridge, MA).

    Techniques: Expressing, Western Blot, Centrifugation, Immunohistochemistry, Staining

    Low E-cadherin is associated with high SNAI1 and prostasphere formation. (A-B) E-cadherin and SNAI1 expression was analyzed in RWPE-1, WPE1-NA22, WPE1-NB14, and DU-145 cells via immunoblotting and confocal microscopy methods. Representative confocal pictures are shown (at 1500x) where Alexa Fluor 555-red is for E-cadherin, Alexa Fluor 488-green is for SNAI1, while DAPI-blue stains nuclei. (C) RWPE-1, WPE1-NA22, WPE1-NB14, and DU-145 cells were plated on 6 well Corning ultra-low attachment plates in DMEM/F-12(Ham) media containing supplements B27 and N2. Prostasphere formation was measured after 8 days. Representative prostasphere pictures are shown at 100x. Data shown is mean ± SEM of 3 samples. * p ≤ 0.001 (compared to RWPE-1 prostasphere number).

    Journal: Molecular Cancer

    Article Title: SNAI1 is critical for the aggressiveness of prostate cancer cells with low E-cadherin

    doi: 10.1186/1476-4598-13-37

    Figure Lengend Snippet: Low E-cadherin is associated with high SNAI1 and prostasphere formation. (A-B) E-cadherin and SNAI1 expression was analyzed in RWPE-1, WPE1-NA22, WPE1-NB14, and DU-145 cells via immunoblotting and confocal microscopy methods. Representative confocal pictures are shown (at 1500x) where Alexa Fluor 555-red is for E-cadherin, Alexa Fluor 488-green is for SNAI1, while DAPI-blue stains nuclei. (C) RWPE-1, WPE1-NA22, WPE1-NB14, and DU-145 cells were plated on 6 well Corning ultra-low attachment plates in DMEM/F-12(Ham) media containing supplements B27 and N2. Prostasphere formation was measured after 8 days. Representative prostasphere pictures are shown at 100x. Data shown is mean ± SEM of 3 samples. * p ≤ 0.001 (compared to RWPE-1 prostasphere number).

    Article Snippet: SNAI1, N-cadherin, OB-cadherin and TATA-binding protein (TBP) antibodies were from Abcam (Cambridge, MA).

    Techniques: Expressing, Confocal Microscopy

    E-cadherin knock-down enhances the stemness of human PCA PC3 cells. (A-B) Sh-PC3 or ShEC-PC3 cells were plated on 6 well Corning ultra-low attachment plates in DMEM/F-12(Ham) media containing supplements B27 and N2. Prostasphere formation was measured after 5 days. Thereafter, prostaspheres were collected and plated on normal cell culture plate with or without serum and monitored for 5 days. Representative pictures are shown for prostaspheres’ state at day 1, day 3 and day 5. Data shown is mean ± SD of 3–6 samples. * p ≤ 0.001.

    Journal: Molecular Cancer

    Article Title: SNAI1 is critical for the aggressiveness of prostate cancer cells with low E-cadherin

    doi: 10.1186/1476-4598-13-37

    Figure Lengend Snippet: E-cadherin knock-down enhances the stemness of human PCA PC3 cells. (A-B) Sh-PC3 or ShEC-PC3 cells were plated on 6 well Corning ultra-low attachment plates in DMEM/F-12(Ham) media containing supplements B27 and N2. Prostasphere formation was measured after 5 days. Thereafter, prostaspheres were collected and plated on normal cell culture plate with or without serum and monitored for 5 days. Representative pictures are shown for prostaspheres’ state at day 1, day 3 and day 5. Data shown is mean ± SD of 3–6 samples. * p ≤ 0.001.

    Article Snippet: SNAI1, N-cadherin, OB-cadherin and TATA-binding protein (TBP) antibodies were from Abcam (Cambridge, MA).

    Techniques: Cell Culture

    E-cadherin knock-down increases the expression of stemness, EMT, and bone metastasis biomarkers in PC3 cells . (A-B) Sh-PC3 or ShEC-PC3 cells were collected at similar confluency and total cell lysates were prepared and analyzed for the protein expression of E-cadherin, CD44, cleaved Notch-1, Egr-1, Vimentin, Integrin β3, N-cadherin, OB-cadherin, CXCR4, uPA, RANKL, and RunX2. Tubulin and β-actin were used as loading controls.

    Journal: Molecular Cancer

    Article Title: SNAI1 is critical for the aggressiveness of prostate cancer cells with low E-cadherin

    doi: 10.1186/1476-4598-13-37

    Figure Lengend Snippet: E-cadherin knock-down increases the expression of stemness, EMT, and bone metastasis biomarkers in PC3 cells . (A-B) Sh-PC3 or ShEC-PC3 cells were collected at similar confluency and total cell lysates were prepared and analyzed for the protein expression of E-cadherin, CD44, cleaved Notch-1, Egr-1, Vimentin, Integrin β3, N-cadherin, OB-cadherin, CXCR4, uPA, RANKL, and RunX2. Tubulin and β-actin were used as loading controls.

    Article Snippet: SNAI1, N-cadherin, OB-cadherin and TATA-binding protein (TBP) antibodies were from Abcam (Cambridge, MA).

    Techniques: Expressing

    Expression of stemness, EMT, and bone metastasis biomarkers in Sh-PC3 and ShEC-PC3 xenograft tissues . Sh-PC3 and ShEC-PC3 xenograft tissues were analyzed for the expression of E-cadherin, CD44, Notch1, pSrc-tyr416, β-catenin, CXCR4 and RANKL by IHC as detailed in the methods. Immunoreactivity was analyzed in 5 random areas for each tumor tissue and was scored as 0+ (no staining), 1+ (weak staining), 2+ (moderate staining), 3+ (strong staining), 4+ (very strong staining). IHC scores (as mean ± SEM) are shown as bar diagram of 5–10 samples.

    Journal: Molecular Cancer

    Article Title: SNAI1 is critical for the aggressiveness of prostate cancer cells with low E-cadherin

    doi: 10.1186/1476-4598-13-37

    Figure Lengend Snippet: Expression of stemness, EMT, and bone metastasis biomarkers in Sh-PC3 and ShEC-PC3 xenograft tissues . Sh-PC3 and ShEC-PC3 xenograft tissues were analyzed for the expression of E-cadherin, CD44, Notch1, pSrc-tyr416, β-catenin, CXCR4 and RANKL by IHC as detailed in the methods. Immunoreactivity was analyzed in 5 random areas for each tumor tissue and was scored as 0+ (no staining), 1+ (weak staining), 2+ (moderate staining), 3+ (strong staining), 4+ (very strong staining). IHC scores (as mean ± SEM) are shown as bar diagram of 5–10 samples.

    Article Snippet: SNAI1, N-cadherin, OB-cadherin and TATA-binding protein (TBP) antibodies were from Abcam (Cambridge, MA).

    Techniques: Expressing, Immunohistochemistry, Staining

    E-cadherin knock-down increases the proliferation of human PCA PC3 cells. (A) Multiplication rate of Sh-PC3 and ShEC-PC3 cells was determined by MTT assay. Data shown is mean ± SD of 12 samples. (B) Clone formation by Sh-PC3 and ShEC-PC3 cells was examined in a clonogenic assay as detailed in the methods. Number of clones with more than 50 cells were counted and presented in a bar diagram. Data shown is mean ± SD of 6 samples. (C-D) Sh-PC3 and ShEC-PC3 cells were injected subcutaneously in athymic nude mice, and average tumor volume (mean ± SEM) as a function of time is presented. Tumor tissues were analyzed for proliferation biomarkers (PCNA and Ki-67) by IHC. Percentage of PCNA and Ki-67 positive cells was calculated by counting the number of positive stained cells (brown stained) and the total number of cells at five arbitrarily selected fields from each tumor at 400x magnification. The data shown in the bar diagrams is the mean±SEM of 7–10 samples. *, p ≤ 0.001; #, p ≤ 0.01; $, p ≤ 0.05.

    Journal: Molecular Cancer

    Article Title: SNAI1 is critical for the aggressiveness of prostate cancer cells with low E-cadherin

    doi: 10.1186/1476-4598-13-37

    Figure Lengend Snippet: E-cadherin knock-down increases the proliferation of human PCA PC3 cells. (A) Multiplication rate of Sh-PC3 and ShEC-PC3 cells was determined by MTT assay. Data shown is mean ± SD of 12 samples. (B) Clone formation by Sh-PC3 and ShEC-PC3 cells was examined in a clonogenic assay as detailed in the methods. Number of clones with more than 50 cells were counted and presented in a bar diagram. Data shown is mean ± SD of 6 samples. (C-D) Sh-PC3 and ShEC-PC3 cells were injected subcutaneously in athymic nude mice, and average tumor volume (mean ± SEM) as a function of time is presented. Tumor tissues were analyzed for proliferation biomarkers (PCNA and Ki-67) by IHC. Percentage of PCNA and Ki-67 positive cells was calculated by counting the number of positive stained cells (brown stained) and the total number of cells at five arbitrarily selected fields from each tumor at 400x magnification. The data shown in the bar diagrams is the mean±SEM of 7–10 samples. *, p ≤ 0.001; #, p ≤ 0.01; $, p ≤ 0.05.

    Article Snippet: SNAI1, N-cadherin, OB-cadherin and TATA-binding protein (TBP) antibodies were from Abcam (Cambridge, MA).

    Techniques: MTT Assay, Clonogenic Assay, Clone Assay, Injection, Mouse Assay, Immunohistochemistry, Staining

    Cadherin-8 and cadherin-11 are enriched in postsynaptic densities and cadherin-8 interacts with neuroligin-1. (A) Forebrain tissues were subjected to synaptic fractionation analysis to determine the subcellular localization of cadherin-8 and cadherin-11. Markers (PSD-95 and syntaxin-1) were probed as control for purity of the fractionation. Total: total protein input, P1: nuclear, S1: cytosol/membranes, P2: crude synaptosome, S2: cytosol/light membranes, P3: synaptosome, SPM: synaptic plasma membrane, PSD: postsynaptic density, S3: synaptic vesicles. (B) Quantification of protein enrichment in SPM and PSD fractions compared to total protein input. PSD-95: ** p = 0.0012 between SPM and total, **** p

    Journal: bioRxiv

    Article Title: Altered Expression of Cadherin-8 and Cadherin-11 in Neural Circuit Development: Implications for Autism

    doi: 10.1101/2020.04.24.058438

    Figure Lengend Snippet: Cadherin-8 and cadherin-11 are enriched in postsynaptic densities and cadherin-8 interacts with neuroligin-1. (A) Forebrain tissues were subjected to synaptic fractionation analysis to determine the subcellular localization of cadherin-8 and cadherin-11. Markers (PSD-95 and syntaxin-1) were probed as control for purity of the fractionation. Total: total protein input, P1: nuclear, S1: cytosol/membranes, P2: crude synaptosome, S2: cytosol/light membranes, P3: synaptosome, SPM: synaptic plasma membrane, PSD: postsynaptic density, S3: synaptic vesicles. (B) Quantification of protein enrichment in SPM and PSD fractions compared to total protein input. PSD-95: ** p = 0.0012 between SPM and total, **** p

    Article Snippet: Protein extract (0.5 mg, standardized to 1 mg/ml) was precleared with 50 μl Protein G Sepharose 4 Fast Flow (Millipore Sigma Cat#GE17-0618-01) or rProtein A Sepharose Fast Flow (Millipore Sigma Cat#GE17-1279-01) for 1 hour at 4° C. Precleared supernatant was incubated with 2 μg anti-cadherin-8 (DSHB Cat#CAD8-1), 7 μg anti-cadherin-11 (Thermo Fisher Scientific Cat#32-1700) or 4 μg anti-HA antibodies (Millipore Sigma Cat#H6908), and mouse or rabbit IgGs for 2 hours at 4° C. Samples were precipitated with 50 μl of pre-equilibrated Protein G Sepharose 4 Fast Flow or rProtein A Sepharose Fast Flow for 1 hour at 4°C with gentle mixing.

    Techniques: Fractionation, Protein Enrichment

    Elevated cadherin-8 expression in Cdh11 -/- mice is accompanied by an increase of excitatory synaptic proteins and dendrite complexity. (A) Western blot analysis of the expression levels of cadherin-11 and cadherin-8 in P7 Cdh11 wild-type (WT), heterozygous (HZ) and knockout (KO) mouse brains. Cadherin-11 expression is not detectable in Cdh11 KO brains. Cadherin signals were normalized to GAPDH. Quantification of the expression of (B) cadherin-11 and (C) cadherin-8 in Cdh11 WT, HZ and KO brains. Cdh11: **** p

    Journal: bioRxiv

    Article Title: Altered Expression of Cadherin-8 and Cadherin-11 in Neural Circuit Development: Implications for Autism

    doi: 10.1101/2020.04.24.058438

    Figure Lengend Snippet: Elevated cadherin-8 expression in Cdh11 -/- mice is accompanied by an increase of excitatory synaptic proteins and dendrite complexity. (A) Western blot analysis of the expression levels of cadherin-11 and cadherin-8 in P7 Cdh11 wild-type (WT), heterozygous (HZ) and knockout (KO) mouse brains. Cadherin-11 expression is not detectable in Cdh11 KO brains. Cadherin signals were normalized to GAPDH. Quantification of the expression of (B) cadherin-11 and (C) cadherin-8 in Cdh11 WT, HZ and KO brains. Cdh11: **** p

    Article Snippet: Protein extract (0.5 mg, standardized to 1 mg/ml) was precleared with 50 μl Protein G Sepharose 4 Fast Flow (Millipore Sigma Cat#GE17-0618-01) or rProtein A Sepharose Fast Flow (Millipore Sigma Cat#GE17-1279-01) for 1 hour at 4° C. Precleared supernatant was incubated with 2 μg anti-cadherin-8 (DSHB Cat#CAD8-1), 7 μg anti-cadherin-11 (Thermo Fisher Scientific Cat#32-1700) or 4 μg anti-HA antibodies (Millipore Sigma Cat#H6908), and mouse or rabbit IgGs for 2 hours at 4° C. Samples were precipitated with 50 μl of pre-equilibrated Protein G Sepharose 4 Fast Flow or rProtein A Sepharose Fast Flow for 1 hour at 4°C with gentle mixing.

    Techniques: Expressing, Mouse Assay, Western Blot, Knock-Out

    Cadherin-8 and cadherin-11 show similar temporal and spatial expression patterns. (A) Temporal expression profile of cadherin-8 and cadherin-11 in mouse whole brain harvested at different developmental ages. Adult mice were 5 months old. (B) Line graph of temporal expression of the two cadherins. Values were normalized to P7. Cdh8: **** p

    Journal: bioRxiv

    Article Title: Altered Expression of Cadherin-8 and Cadherin-11 in Neural Circuit Development: Implications for Autism

    doi: 10.1101/2020.04.24.058438

    Figure Lengend Snippet: Cadherin-8 and cadherin-11 show similar temporal and spatial expression patterns. (A) Temporal expression profile of cadherin-8 and cadherin-11 in mouse whole brain harvested at different developmental ages. Adult mice were 5 months old. (B) Line graph of temporal expression of the two cadherins. Values were normalized to P7. Cdh8: **** p

    Article Snippet: Protein extract (0.5 mg, standardized to 1 mg/ml) was precleared with 50 μl Protein G Sepharose 4 Fast Flow (Millipore Sigma Cat#GE17-0618-01) or rProtein A Sepharose Fast Flow (Millipore Sigma Cat#GE17-1279-01) for 1 hour at 4° C. Precleared supernatant was incubated with 2 μg anti-cadherin-8 (DSHB Cat#CAD8-1), 7 μg anti-cadherin-11 (Thermo Fisher Scientific Cat#32-1700) or 4 μg anti-HA antibodies (Millipore Sigma Cat#H6908), and mouse or rabbit IgGs for 2 hours at 4° C. Samples were precipitated with 50 μl of pre-equilibrated Protein G Sepharose 4 Fast Flow or rProtein A Sepharose Fast Flow for 1 hour at 4°C with gentle mixing.

    Techniques: Expressing, Mouse Assay