Journal: Frontiers in Cellular Neuroscience
Article Title: Functional Coupling of Cav2.3 and BK Potassium Channels Regulates Action Potential Repolarization and Short-Term Plasticity in the Mouse Hippocampus
Figure Lengend Snippet: Cav2.3 knockout (KO) CA1 pyramidal cells are hyperexcitable. (A) Resting membrane potential (Vrest; A1 ) and cell capacitance (A2) , measured after break-in. (B) Input resistance quantified as the slope of an IV-curve for hyperpolarizing current steps from −160 to −20 pA. Insets represent averaged voltage traces for wildtype (WT; black) and KO (red) recordings during current injection. (C) Hyperpolarization activated current I H , measured as sag-percentage from maximum voltage deflection (C1) and as the slope of the rebound potential after the end of a series of hyperpolarizing current injections plotted against the steady-state voltage during the current injection (C2) . (D) Firing frequency of action potentials (APs) in CA1 pyramidal neurons of Cav2.3 KO and WT animals, elicited by current steps from Vrest, by injection of positive or negative current in steps of 20 pA. Inset shows example recordings for injections of +200 pA WT (black traces) or Cav2.3 KO (red traces). n = 17 for WT, and 21 for KO. All data shown as mean ± SEM, with Student’s T -Test to probe for significance (n.s. = not significant, * p
Article Snippet: The separated proteins were immuonoblotted using Cav2.3 (1:1,000, Synaptic Systems, Germany) or BK antibody (1:2,000, Alomone Labs, Israel) and visualized by Alexa Fluor 680 secondary antibody (1:10,000, Invitrogen, Carlsbad, CA, USA) and Alexa Fluor 800 secondary antibody (1:5,000, Rockland, Knox County, MA, USA).
Techniques: Knock-Out, Injection