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  • 99
    Charles River Laboratories old female c57bl 6 mice
    Detection of hUC-MSC by quantitative real-time PCR assay for human GAPDH. Human GAPDH assessed in RNA extracted from cultured hUC-MSC prior to infusion or from lung tissue of <t>C57BL/6</t> mice (n = 5 per group) receiving endotracheal bleomycin followed by intravenous hUC-MSC (bleomycin+hUC-MSC). Days 8, 14, 21 refer to bleomycin administration, and correspond, respectively, to 1, 7 and 14 days after the second hUC-MSC infusion. Results are expressed as mean ± SD (n = 5 per group) and are representative of three independent experiments performed in triplicate.
    Old Female C57bl 6 Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 191 article reviews
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    old female c57bl 6 mice - by Bioz Stars, 2019-10
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    85
    Japan SLC inc male c57bl 6 j mice
    Organ fatty acid compositions in diet-induced obese C57BL/6 J mice fed the control, EPA or LCMUFA diet for 8 weeks. Fatty acid composition in liver ( a ) and epididymal adipose tissue ( b ) at the end of 8 weeks. Values are means ± SEM,  n  = 10. Significantly different mean values ( P
    Male C57bl 6 J Mice, supplied by Japan SLC inc, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/male c57bl 6 j mice/product/Japan SLC inc
    Average 85 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Detection of hUC-MSC by quantitative real-time PCR assay for human GAPDH. Human GAPDH assessed in RNA extracted from cultured hUC-MSC prior to infusion or from lung tissue of C57BL/6 mice (n = 5 per group) receiving endotracheal bleomycin followed by intravenous hUC-MSC (bleomycin+hUC-MSC). Days 8, 14, 21 refer to bleomycin administration, and correspond, respectively, to 1, 7 and 14 days after the second hUC-MSC infusion. Results are expressed as mean ± SD (n = 5 per group) and are representative of three independent experiments performed in triplicate.

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: Detection of hUC-MSC by quantitative real-time PCR assay for human GAPDH. Human GAPDH assessed in RNA extracted from cultured hUC-MSC prior to infusion or from lung tissue of C57BL/6 mice (n = 5 per group) receiving endotracheal bleomycin followed by intravenous hUC-MSC (bleomycin+hUC-MSC). Days 8, 14, 21 refer to bleomycin administration, and correspond, respectively, to 1, 7 and 14 days after the second hUC-MSC infusion. Results are expressed as mean ± SD (n = 5 per group) and are representative of three independent experiments performed in triplicate.

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Real-time Polymerase Chain Reaction, Cell Culture, Mouse Assay

    Decrease of arginase-I positive cells in bleomycin-injured lung tissue upon administration of hUC-MSC. Macrophage infiltration in mouse lungs at days 8 ( A-D ) and 21 ( E-H ) after endotracheal injection of sterile saline (saline) ( A, E ) or bleomycin (bleomycin) ( B, F ), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) ( D, H ) or sterile saline (bleomycin+saline) ( C, G ). Lung sections obtained from C57BL/6 mice (n = 8 per group) were immunostained with anti-Arginase I antibody. At each time point, the number of immunoreactive macrophages infiltrating the lungs is significantly diminished by hUC-MSC infusion in comparison to bleomycin-treated mice. Representative microscopic images (40× magnification) of three independent experiments are shown. ( I) Cell count of arginase I positive macrophages in C57BL/6 mouse lung sections. Results are mean ± SD of positive immunostained cell count per sample (n = 8 per group) and are representative of three independent experiments. ** = P

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: Decrease of arginase-I positive cells in bleomycin-injured lung tissue upon administration of hUC-MSC. Macrophage infiltration in mouse lungs at days 8 ( A-D ) and 21 ( E-H ) after endotracheal injection of sterile saline (saline) ( A, E ) or bleomycin (bleomycin) ( B, F ), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) ( D, H ) or sterile saline (bleomycin+saline) ( C, G ). Lung sections obtained from C57BL/6 mice (n = 8 per group) were immunostained with anti-Arginase I antibody. At each time point, the number of immunoreactive macrophages infiltrating the lungs is significantly diminished by hUC-MSC infusion in comparison to bleomycin-treated mice. Representative microscopic images (40× magnification) of three independent experiments are shown. ( I) Cell count of arginase I positive macrophages in C57BL/6 mouse lung sections. Results are mean ± SD of positive immunostained cell count per sample (n = 8 per group) and are representative of three independent experiments. ** = P

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Injection, Mouse Assay, Cell Counting

    Intravenous administration of human fibroblasts does not prevent the development of bleomycin-induced lung injury. Histology ( A-B ), collagen content ( C-D ) and macrophage infiltration ( E-H ) of mouse lungs 21 days after bleomycin endotracheal injection followed by human fibroblasts intravenous infusion. Lung sections obtained from C57BL/6 mice (n = 8 per group) that received endotracheal bleomycin only (bleomycin) or endotracheal bleomycin followed by intravenous human fibroblasts (bleomycin+fibroblasts) were stained with H E ( A-B ), Picrosirius Red ( C-D ), anti-galectin-3 ( E-F ) or anti-arginase I ( G-H ) antibodies. Representative microscopic images (10× or 40× magnification) of three independent experiments are shown. Quantitative real-time PCR gene expression analysis of Col1A1, TGFβ and αSMA ( I ), and IL-1β, IL-2, IL-6 and IL-10 ( J ) in whole lung mRNA obtained at day 21 from C57BL/6 mice receiving the aforementioned treatments. Results are expressed as mean ± SD (n = 5 per group) and are representative of three independent experiments performed in triplicate. ( K ) Quantification of galectin-3 and arginase-I positive macrophages in C57BL/6 mouse lungs 21 days after bleomycin injection with or without subsequent human fibroblast infusion. Results are mean ± SD of positive immunostained cell count per sample (n = 8 per group) and are representative of three independent experiments.

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: Intravenous administration of human fibroblasts does not prevent the development of bleomycin-induced lung injury. Histology ( A-B ), collagen content ( C-D ) and macrophage infiltration ( E-H ) of mouse lungs 21 days after bleomycin endotracheal injection followed by human fibroblasts intravenous infusion. Lung sections obtained from C57BL/6 mice (n = 8 per group) that received endotracheal bleomycin only (bleomycin) or endotracheal bleomycin followed by intravenous human fibroblasts (bleomycin+fibroblasts) were stained with H E ( A-B ), Picrosirius Red ( C-D ), anti-galectin-3 ( E-F ) or anti-arginase I ( G-H ) antibodies. Representative microscopic images (10× or 40× magnification) of three independent experiments are shown. Quantitative real-time PCR gene expression analysis of Col1A1, TGFβ and αSMA ( I ), and IL-1β, IL-2, IL-6 and IL-10 ( J ) in whole lung mRNA obtained at day 21 from C57BL/6 mice receiving the aforementioned treatments. Results are expressed as mean ± SD (n = 5 per group) and are representative of three independent experiments performed in triplicate. ( K ) Quantification of galectin-3 and arginase-I positive macrophages in C57BL/6 mouse lungs 21 days after bleomycin injection with or without subsequent human fibroblast infusion. Results are mean ± SD of positive immunostained cell count per sample (n = 8 per group) and are representative of three independent experiments.

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Injection, Mouse Assay, Staining, Real-time Polymerase Chain Reaction, Expressing, Cell Counting

    Decrease of galectin-3 positive cells in bleomycin-injured lung tissue upon administration of hUC-MSC. Macrophage infiltration in mouse lungs at days 8 ( A-D ) and 21 ( E-H ) after endotracheal injection of sterile saline (saline) ( A, E ) or bleomycin (bleomycin) ( B, F ), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) ( D, H ) or sterile saline (bleomycin+saline) ( C, G ). Lung sections obtained from C57BL/6 mice (n = 8 per group) were immunostained with anti-galectin-3 antibody. At each time point, the number of immunoreactive macrophages infiltrating the lungs are significantly decreased by hUC-MSC infusion in comparison to bleomycin-treated mice. Representative microscopic images (40× magnification) of three independent experiments are shown. ( I) Cell count of galectin-3 positive macrophages in C57BL/6 mouse lung sections, and ( J ) quantitative real-time PCR analysis of galectin-3 gene expression in whole lung mRNA, obtained at days 8 and 21. Results are expressed as mean ± SD of positively immunostained cell count per sample (n = 5 per group) and are representative of three independent experiments. * = P

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: Decrease of galectin-3 positive cells in bleomycin-injured lung tissue upon administration of hUC-MSC. Macrophage infiltration in mouse lungs at days 8 ( A-D ) and 21 ( E-H ) after endotracheal injection of sterile saline (saline) ( A, E ) or bleomycin (bleomycin) ( B, F ), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) ( D, H ) or sterile saline (bleomycin+saline) ( C, G ). Lung sections obtained from C57BL/6 mice (n = 8 per group) were immunostained with anti-galectin-3 antibody. At each time point, the number of immunoreactive macrophages infiltrating the lungs are significantly decreased by hUC-MSC infusion in comparison to bleomycin-treated mice. Representative microscopic images (40× magnification) of three independent experiments are shown. ( I) Cell count of galectin-3 positive macrophages in C57BL/6 mouse lung sections, and ( J ) quantitative real-time PCR analysis of galectin-3 gene expression in whole lung mRNA, obtained at days 8 and 21. Results are expressed as mean ± SD of positively immunostained cell count per sample (n = 5 per group) and are representative of three independent experiments. * = P

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Injection, Mouse Assay, Cell Counting, Real-time Polymerase Chain Reaction, Expressing

    Detection of hUC-MSC in lung tissue by vimentin IHC. Lung sections obtained from C57BL/6 mice (n = 8 per group) receiving endotracheal bleomycin only ( A , B , E ) or endotracheal bleomycin followed by intravenous hUC-MSC ( C , D , F ) were immunostained with anti-human vimentin antibody. ( C,D) Lung sections from hUC-MSC-treated mice show at day 8 (i.e. one day after second hUC-MSC infusion) numerous immunoreactive cells, distributed mainly in the septa and in the peribronchial tissue (200X), that exhibit at higher magnification (400X) a polygonal or stellate shape and enlarged nuclei (arrows). ( A,B ) In lung sections from mice treated with bleomycin only, the anti-human vimentin staining is extracellular and unspecific; no cells with polygonal or stellate shape reactive for human vimentin are evident (200X and 400X). ( F ) Lung sections from hUC-MSC-treated mice show at day 21 (i.e. 14 days after second hUC-MSC infusion) normal alveolar architecture with no fibrosis or inflammation, which are evident in the untreated mice ( E ), and only few vimentin-positive cells (arrows).

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: Detection of hUC-MSC in lung tissue by vimentin IHC. Lung sections obtained from C57BL/6 mice (n = 8 per group) receiving endotracheal bleomycin only ( A , B , E ) or endotracheal bleomycin followed by intravenous hUC-MSC ( C , D , F ) were immunostained with anti-human vimentin antibody. ( C,D) Lung sections from hUC-MSC-treated mice show at day 8 (i.e. one day after second hUC-MSC infusion) numerous immunoreactive cells, distributed mainly in the septa and in the peribronchial tissue (200X), that exhibit at higher magnification (400X) a polygonal or stellate shape and enlarged nuclei (arrows). ( A,B ) In lung sections from mice treated with bleomycin only, the anti-human vimentin staining is extracellular and unspecific; no cells with polygonal or stellate shape reactive for human vimentin are evident (200X and 400X). ( F ) Lung sections from hUC-MSC-treated mice show at day 21 (i.e. 14 days after second hUC-MSC infusion) normal alveolar architecture with no fibrosis or inflammation, which are evident in the untreated mice ( E ), and only few vimentin-positive cells (arrows).

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Immunohistochemistry, Mouse Assay, Staining

    hUC-MSC down-regulate bleomycin-induced lung fibrosis. Collagen content in mouse lungs 8 days ( A-D ), 14 days ( E-H ) and 21 days ( I-L ) after endotracheal injection of sterile saline (saline) or bleomycin (bleomycin), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) or sterile saline (bleomycin+saline). Lung sections obtained from C57BL/6 mice (n = 8 per group) were stained with Picrosirius Red. Controls ( A,E,I ) demonstrated normal lung architecture. 8 days post bleomycin injury, initial thickening of the alveoli and septa was observed ( B ). Collagen deposition progressively increased from day 8 to 21, with progressive distortion of lung architecture and formation of fibrotic foci ( F,J ). At each time point, bleomycin-induced alterations were significantly attenuated by hUC-MSC treatment ( D,H,L ), but not by saline ( C,G,K ). Representative microscopic images (10× magnification) of three independent experiments are shown. ( M ) The Ashcroft fibrosis score of lung sections obtained from C57BL/6 mice (n = 8 per group) that received the aforementioned treatments was calculated. Results are mean ± SD (n = 8 per group) and are representative of three independent experiments. * = P

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: hUC-MSC down-regulate bleomycin-induced lung fibrosis. Collagen content in mouse lungs 8 days ( A-D ), 14 days ( E-H ) and 21 days ( I-L ) after endotracheal injection of sterile saline (saline) or bleomycin (bleomycin), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) or sterile saline (bleomycin+saline). Lung sections obtained from C57BL/6 mice (n = 8 per group) were stained with Picrosirius Red. Controls ( A,E,I ) demonstrated normal lung architecture. 8 days post bleomycin injury, initial thickening of the alveoli and septa was observed ( B ). Collagen deposition progressively increased from day 8 to 21, with progressive distortion of lung architecture and formation of fibrotic foci ( F,J ). At each time point, bleomycin-induced alterations were significantly attenuated by hUC-MSC treatment ( D,H,L ), but not by saline ( C,G,K ). Representative microscopic images (10× magnification) of three independent experiments are shown. ( M ) The Ashcroft fibrosis score of lung sections obtained from C57BL/6 mice (n = 8 per group) that received the aforementioned treatments was calculated. Results are mean ± SD (n = 8 per group) and are representative of three independent experiments. * = P

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Injection, Mouse Assay, Staining

    Protein expression of cytokines and matrix components in lung tissue assessed by Western blotting. Collagen Col1A1 ( A ), TGFβ ( B ), IL-1β ( C ), IL-2 ( D ), IL-6 ( E ) and IL-10 ( F ) protein levels in whole lung tissue lysates obtained from C57BL/6 mice 21 days after endotracheal injection of sterile saline (saline) or bleomycin (bleo), the latter also followed by intravenous infusion of hUC-MSC (bleo+hUC-MSC) or sterile saline (bleo+saline). Results are representative of three independent experiments. Densitometric analysis of protein bands is normalized to actin and expressed as mean ± SD (n = 5 per group). ** = P

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: Protein expression of cytokines and matrix components in lung tissue assessed by Western blotting. Collagen Col1A1 ( A ), TGFβ ( B ), IL-1β ( C ), IL-2 ( D ), IL-6 ( E ) and IL-10 ( F ) protein levels in whole lung tissue lysates obtained from C57BL/6 mice 21 days after endotracheal injection of sterile saline (saline) or bleomycin (bleo), the latter also followed by intravenous infusion of hUC-MSC (bleo+hUC-MSC) or sterile saline (bleo+saline). Results are representative of three independent experiments. Densitometric analysis of protein bands is normalized to actin and expressed as mean ± SD (n = 5 per group). ** = P

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Expressing, Western Blot, Mouse Assay, Injection

    Detection of hUC-MSC in lung tissue by HLA-1 and CD105 IHC. Lung sections obtained from C57BL/6 mice receiving endotracheal bleomycin followed by intravenous hUC-MSC were immunostained with anti-HLA-1 ( A-B ) or anti-CD105 ( C-D ) antibodies. Representative microscopic images (200X and 400X) of three independent experiments are shown,. Lung sections from hUC-MSC-treated mice show at day 21 (i.e. 14 days after second hUC-MSC infusion) normal alveolar architecture with no fibrosis or inflammation, and only few positive cells (arrows, 400X).

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: Detection of hUC-MSC in lung tissue by HLA-1 and CD105 IHC. Lung sections obtained from C57BL/6 mice receiving endotracheal bleomycin followed by intravenous hUC-MSC were immunostained with anti-HLA-1 ( A-B ) or anti-CD105 ( C-D ) antibodies. Representative microscopic images (200X and 400X) of three independent experiments are shown,. Lung sections from hUC-MSC-treated mice show at day 21 (i.e. 14 days after second hUC-MSC infusion) normal alveolar architecture with no fibrosis or inflammation, and only few positive cells (arrows, 400X).

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Immunohistochemistry, Mouse Assay

    Immunohistochemical determination of α-SMA in lung tissue. Peribronchial and perivascular deposition of α-SMA in C57BL/6 mouse lungs at day 21 after injection of endotracheal sterile saline only ( A ), endotracheal bleomycin only ( B ), endotracheal bleomycin followed by intravenous sterile saline ( C ) or endotracheal bleomycin followed by intravenous hUC-MSC ( D ). Lung sections were immunostained with anti-α-SMA antibody. Representative microscopic images (40× magnification) of three independent experiments are shown.

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: Immunohistochemical determination of α-SMA in lung tissue. Peribronchial and perivascular deposition of α-SMA in C57BL/6 mouse lungs at day 21 after injection of endotracheal sterile saline only ( A ), endotracheal bleomycin only ( B ), endotracheal bleomycin followed by intravenous sterile saline ( C ) or endotracheal bleomycin followed by intravenous hUC-MSC ( D ). Lung sections were immunostained with anti-α-SMA antibody. Representative microscopic images (40× magnification) of three independent experiments are shown.

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Immunohistochemistry, Injection

    hUC-MSC do not induce any biological effects on control mice treated with endotracheal saline. Histology ( A-B ), collagen content ( C-D ) and macrophage infiltration ( E-H ) of mouse lungs 21 days after sterile saline endotracheal injection followed by hUC-MSC intravenous infusion. Lung sections obtained from C57BL/6 mice (n = 8 per group) that received endotracheal sterile saline only (saline) or endotracheal sterile saline followed by intravenous hUC-MSC (saline+hUC-MSC) were stained with H E ( A-B ), Picrosirius Red ( C-D ), anti- galectin-3 ( E-F ) and anti-arginase I ( G-H ) antibodies. Representative microscopic images (10× or 40× magnification) of three independent experiments are shown. Quantitative real-time PCR gene expression analysis of Col1A1, TGFβ and αSMA ( I ), and IL-1β, IL-2, IL-6 and IL-10 ( J ) in whole lung mRNA obtained at day 21 from C57BL/6 mice receiving the aforementioned treatments. Results are expressed as mean ± SD (n = 5 per group) and are representative of three independent experiments performed in triplicate. ( K ) Quantification of galectin-3 and arginase I positive macrophages in C57BL/6 mouse lungs 21 days after saline injection with or without subsequent hUC-MSC infusion. Results are mean ± SD of positive immunostained cell count per sample (n = 8 per group) and are representative of three independent experiments.

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: hUC-MSC do not induce any biological effects on control mice treated with endotracheal saline. Histology ( A-B ), collagen content ( C-D ) and macrophage infiltration ( E-H ) of mouse lungs 21 days after sterile saline endotracheal injection followed by hUC-MSC intravenous infusion. Lung sections obtained from C57BL/6 mice (n = 8 per group) that received endotracheal sterile saline only (saline) or endotracheal sterile saline followed by intravenous hUC-MSC (saline+hUC-MSC) were stained with H E ( A-B ), Picrosirius Red ( C-D ), anti- galectin-3 ( E-F ) and anti-arginase I ( G-H ) antibodies. Representative microscopic images (10× or 40× magnification) of three independent experiments are shown. Quantitative real-time PCR gene expression analysis of Col1A1, TGFβ and αSMA ( I ), and IL-1β, IL-2, IL-6 and IL-10 ( J ) in whole lung mRNA obtained at day 21 from C57BL/6 mice receiving the aforementioned treatments. Results are expressed as mean ± SD (n = 5 per group) and are representative of three independent experiments performed in triplicate. ( K ) Quantification of galectin-3 and arginase I positive macrophages in C57BL/6 mouse lungs 21 days after saline injection with or without subsequent hUC-MSC infusion. Results are mean ± SD of positive immunostained cell count per sample (n = 8 per group) and are representative of three independent experiments.

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Mouse Assay, Injection, Staining, Real-time Polymerase Chain Reaction, Expressing, Cell Counting

    hUC-MSC down-regulate bleomycin-induced lung inflammation. Histology of mouse lungs 8 days ( A-D ), 14 days ( E-H ) and 21 days ( I-L ) after endotracheal injection of sterile saline (saline) or bleomycin (bleomycin), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) or sterile saline (bleomycin+saline). Lung sections obtained from C57BL/6 mice (n = 8 per group) were stained with H E. Controls ( A,E,I ) demonstrated normal lung architecture. 8 days post bleomycin injury, peribronchial and perivascular inflammatory infiltrates were observed ( B ). Alveolar and interstitial infiltrates progressively increased from day 8 to 21, with progressive distortion of lung architecture and formation of fibrotic foci ( F,J ). At each time point, bleomycin-induced alterations were significantly attenuated by hUC-MSC treatment ( D,H,L ), but not by saline ( C,G,K ). Representative microscopic images (10× magnification) of three independent experiments are shown. ( M ) The histopathological inflammatory score of lung sections obtained from C57BL/6 mice (n = 8 per group) that received the aforementioned treatments was calculated. Results are mean ± SD (n = 8 per group) and are representative of three independent experiments. * = P

    Journal: PLoS ONE

    Article Title: Mesenchymal stromal cells from human umbilical cord prevent the development of lung fibrosis in immunocompetent mice

    doi: 10.1371/journal.pone.0196048

    Figure Lengend Snippet: hUC-MSC down-regulate bleomycin-induced lung inflammation. Histology of mouse lungs 8 days ( A-D ), 14 days ( E-H ) and 21 days ( I-L ) after endotracheal injection of sterile saline (saline) or bleomycin (bleomycin), the latter also followed by intravenous infusion of hUC-MSC (bleomycin+hUC-MSC) or sterile saline (bleomycin+saline). Lung sections obtained from C57BL/6 mice (n = 8 per group) were stained with H E. Controls ( A,E,I ) demonstrated normal lung architecture. 8 days post bleomycin injury, peribronchial and perivascular inflammatory infiltrates were observed ( B ). Alveolar and interstitial infiltrates progressively increased from day 8 to 21, with progressive distortion of lung architecture and formation of fibrotic foci ( F,J ). At each time point, bleomycin-induced alterations were significantly attenuated by hUC-MSC treatment ( D,H,L ), but not by saline ( C,G,K ). Representative microscopic images (10× magnification) of three independent experiments are shown. ( M ) The histopathological inflammatory score of lung sections obtained from C57BL/6 mice (n = 8 per group) that received the aforementioned treatments was calculated. Results are mean ± SD (n = 8 per group) and are representative of three independent experiments. * = P

    Article Snippet: Twelve- to sixteen-week old female C57BL/6 mice (Charles River Laboratories) were used in all experiments.

    Techniques: Injection, Mouse Assay, Staining

    Cellular changes in spleens collected from serially sampled mice. BALB/c (A) and C57BL/6 mice (B) were exposed to B . pseudomallei MSHR5855. In most cases N = 5.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Cellular changes in spleens collected from serially sampled mice. BALB/c (A) and C57BL/6 mice (B) were exposed to B . pseudomallei MSHR5855. In most cases N = 5.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Mouse Assay

    Heat map depicting the average severity observed in BALB/c and C57BL/6 mice after exposure to aerosolized B . pseudomallei .

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Heat map depicting the average severity observed in BALB/c and C57BL/6 mice after exposure to aerosolized B . pseudomallei .

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Mouse Assay

    Bacterial burden observed during a serial sampling study of mice exposed to aerosolized B . pseudomallei HBPUB10134a. BALB/c mice are depicted in the top row (blue) and C57BL/6 mice are depicted in the bottom row (orange) . The CFU determinations for blood, Spleen, and lung are shown and the geometric mean is depicted with a horizontal bar.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Bacterial burden observed during a serial sampling study of mice exposed to aerosolized B . pseudomallei HBPUB10134a. BALB/c mice are depicted in the top row (blue) and C57BL/6 mice are depicted in the bottom row (orange) . The CFU determinations for blood, Spleen, and lung are shown and the geometric mean is depicted with a horizontal bar.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Sampling, Mouse Assay

    Representative Lung Pathology in mice after exposure to aerosolized B . pseudomallei . (A) BALB/c mouse exposed to 1106a (Day 9). The bronchus, bronchioles and surrounding alveoli are distended by suppurative inflammation and macrophages (arrow), with variable amount of edema fluid. (B) BALB/c mouse exposed to HBPUB10134a (Day 10). The bronchus, bronchioles and surrounding alveoli are severely distended or replaced by abundant suppurative inflammation with fewer macrophages (arrow). Edema fluid and hemorrhage also variably expand the alveoli and to a lesser extent conducting airways. Pulmonary architecture is replaced by necrotic debris in some areas (asterisk). (C) BALB/c mouse exposed to MSHR5855 (Day 10). The bronchus, bronchioles and surrounding alveoli are distended by suppurative inflammation and macrophages (arrow). There is marked edema fluid within the alveoli. A pyogranuloma is present with adjacent areas of necrosis and hemorrhage (asterisk). Several vessels are occluded by fibrin thrombi (double arrow). There is moderate BALT hyperplasia. The less affected area has expanded interstitium by neutrophils and macrophages. (D) C57BL/6 mouse exposed to 1106a (Day 9). There is minimal to mild interstitial expansion by neutrophils and macrophages (arrow); and mild BALT hyperplasia. (E) C57BL/6 mouse exposed to HBPUB10134a (Day 10). The interstitium is moderately expanded by neutrophils and macrophages. A focal area of necrosis replaces pulmonary architecture and resembles a poorly formed pyogranuloma (arrow). There is moderate BALT hyperplasia. (F) C57BL/6 mouse exposed to MSHR5855 (Day 10). There is mild expansion of the interstitium by neutrophils and macrophages. There are extensive areas of hemorrhage expanding alveoli (arrow). There are small foci (not pictured) of aggregated macrophages with few MNGCs.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Representative Lung Pathology in mice after exposure to aerosolized B . pseudomallei . (A) BALB/c mouse exposed to 1106a (Day 9). The bronchus, bronchioles and surrounding alveoli are distended by suppurative inflammation and macrophages (arrow), with variable amount of edema fluid. (B) BALB/c mouse exposed to HBPUB10134a (Day 10). The bronchus, bronchioles and surrounding alveoli are severely distended or replaced by abundant suppurative inflammation with fewer macrophages (arrow). Edema fluid and hemorrhage also variably expand the alveoli and to a lesser extent conducting airways. Pulmonary architecture is replaced by necrotic debris in some areas (asterisk). (C) BALB/c mouse exposed to MSHR5855 (Day 10). The bronchus, bronchioles and surrounding alveoli are distended by suppurative inflammation and macrophages (arrow). There is marked edema fluid within the alveoli. A pyogranuloma is present with adjacent areas of necrosis and hemorrhage (asterisk). Several vessels are occluded by fibrin thrombi (double arrow). There is moderate BALT hyperplasia. The less affected area has expanded interstitium by neutrophils and macrophages. (D) C57BL/6 mouse exposed to 1106a (Day 9). There is minimal to mild interstitial expansion by neutrophils and macrophages (arrow); and mild BALT hyperplasia. (E) C57BL/6 mouse exposed to HBPUB10134a (Day 10). The interstitium is moderately expanded by neutrophils and macrophages. A focal area of necrosis replaces pulmonary architecture and resembles a poorly formed pyogranuloma (arrow). There is moderate BALT hyperplasia. (F) C57BL/6 mouse exposed to MSHR5855 (Day 10). There is mild expansion of the interstitium by neutrophils and macrophages. There are extensive areas of hemorrhage expanding alveoli (arrow). There are small foci (not pictured) of aggregated macrophages with few MNGCs.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Mouse Assay

    Bacterial burden observed during a serial sampling study of mice exposed to aerosolized B . pseudomallei MSHR5855. BALB/c mice are depicted in the top row (blue) and C57BL/6 mice are depicted in the bottom row (orange) . The CFU determinations for blood, Spleen, and lung are shown and the geometric mean is depicted with a horizontal bar.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Bacterial burden observed during a serial sampling study of mice exposed to aerosolized B . pseudomallei MSHR5855. BALB/c mice are depicted in the top row (blue) and C57BL/6 mice are depicted in the bottom row (orange) . The CFU determinations for blood, Spleen, and lung are shown and the geometric mean is depicted with a horizontal bar.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Sampling, Mouse Assay

    Changes in cytokine/chemokine levels in spleen homogenates from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei . Spleen homogenates were examined for cytokine/chemokine expression at different times after exposure of BALB/c or C57BL/6 mice to HBPUB10134a, or MSHR5855, or 1106a. For each time point after exposure N = 5, and geometric means were determined. Fold changes in cytokines/chemokines in spleen homogenates were determined by dividing the amount in geometric means (pg/ml) determined after exposure at a specific time by the amount found in lung homogenates from naïve, normal BALB/c or C57BL/6 mice (pg/ml). For cytokines/chemokines in spleens from naïve BALB/c mice, N = 4 and for C57BL/6 mice, N = 5. The numbers on the left of each panel shows the days after exposure. The bar on the right of each heat map shows the fold-change in expression of each cytokine/chemokine after time of exposure.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Changes in cytokine/chemokine levels in spleen homogenates from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei . Spleen homogenates were examined for cytokine/chemokine expression at different times after exposure of BALB/c or C57BL/6 mice to HBPUB10134a, or MSHR5855, or 1106a. For each time point after exposure N = 5, and geometric means were determined. Fold changes in cytokines/chemokines in spleen homogenates were determined by dividing the amount in geometric means (pg/ml) determined after exposure at a specific time by the amount found in lung homogenates from naïve, normal BALB/c or C57BL/6 mice (pg/ml). For cytokines/chemokines in spleens from naïve BALB/c mice, N = 4 and for C57BL/6 mice, N = 5. The numbers on the left of each panel shows the days after exposure. The bar on the right of each heat map shows the fold-change in expression of each cytokine/chemokine after time of exposure.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Mouse Assay, Expressing

    Changes in cytokine/chemokine levels in lung homogenates from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei . Lung homogenates were examined for cytokine/chemokine expression at different times after exposure of BALB/c or C57BL/6 mice to HBPUB10134a, or MSHR5855, or 1106a. For each time point after exposure N = 5, and geometric means were determined. Fold changes in cytokines/chemokines in sera were determined by dividing the amount in geometric means (pg/ml) determined after exposure at a specific time by the amount found in spleen extracts from naïve, normal BALB/c or C57BL/6 mice (pg/ml). For cytokines/chemokines in lungs from naïve BALB/c mice, N = 5, and for C57BL/6 mice, N = 5. The numbers on the left of each panel shows the days after exposure. The bar on the right of each heat map shows the fold-change in expression of each cytokine/chemokine after time of exposure.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Changes in cytokine/chemokine levels in lung homogenates from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei . Lung homogenates were examined for cytokine/chemokine expression at different times after exposure of BALB/c or C57BL/6 mice to HBPUB10134a, or MSHR5855, or 1106a. For each time point after exposure N = 5, and geometric means were determined. Fold changes in cytokines/chemokines in sera were determined by dividing the amount in geometric means (pg/ml) determined after exposure at a specific time by the amount found in spleen extracts from naïve, normal BALB/c or C57BL/6 mice (pg/ml). For cytokines/chemokines in lungs from naïve BALB/c mice, N = 5, and for C57BL/6 mice, N = 5. The numbers on the left of each panel shows the days after exposure. The bar on the right of each heat map shows the fold-change in expression of each cytokine/chemokine after time of exposure.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Mouse Assay, Expressing

    Bacterial burden observed during a serial sampling study of mice exposed to aerosolized B . pseudomallei 1106a. BALB/c mice are depicted in the top row (blue) and C57BL/6 mice are depicted in the bottom row (orange) . The CFU determinations for blood, Spleen, and lung are shown and the geometric mean is depicted with a horizontal bar.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Bacterial burden observed during a serial sampling study of mice exposed to aerosolized B . pseudomallei 1106a. BALB/c mice are depicted in the top row (blue) and C57BL/6 mice are depicted in the bottom row (orange) . The CFU determinations for blood, Spleen, and lung are shown and the geometric mean is depicted with a horizontal bar.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Sampling, Mouse Assay

    Cellular changes in spleens collected from serially sampled mice. BALB/c (A) and C57BL/6 mice (B) were exposed to B . pseudomallei 1106a. In most cases N = 5.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Cellular changes in spleens collected from serially sampled mice. BALB/c (A) and C57BL/6 mice (B) were exposed to B . pseudomallei 1106a. In most cases N = 5.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Mouse Assay

    Changes in cytokine/chemokine levels in sera from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei . Sera were examined for cytokine/chemokine expression at different times after exposure of BALB/c or C57BL/6 mice to HBPUB10134a, or MSHR5855, or 1106a. For each time point after exposure N = 5, and geometric means were determined. Fold changes in cytokines/chemokines in sera were determined by dividing the amount in geometric means (pg/ml) determined after exposure at a specific time by the amount found in sera from naïve, normal BALB/c or C57BL/6 mice (pg/ml). For cytokines/chemokines in sera from naïve BALB/c mice, N = 6, and from C57BL/6 mice, N = 5. The number on the left of each panel shows the days after exposure. The bar on the right of each heat map shows the fold-change in expression of each cytokine/chemokine after time of exposure.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Changes in cytokine/chemokine levels in sera from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei . Sera were examined for cytokine/chemokine expression at different times after exposure of BALB/c or C57BL/6 mice to HBPUB10134a, or MSHR5855, or 1106a. For each time point after exposure N = 5, and geometric means were determined. Fold changes in cytokines/chemokines in sera were determined by dividing the amount in geometric means (pg/ml) determined after exposure at a specific time by the amount found in sera from naïve, normal BALB/c or C57BL/6 mice (pg/ml). For cytokines/chemokines in sera from naïve BALB/c mice, N = 6, and from C57BL/6 mice, N = 5. The number on the left of each panel shows the days after exposure. The bar on the right of each heat map shows the fold-change in expression of each cytokine/chemokine after time of exposure.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Mouse Assay, Expressing

    The IgG antibody response in BALB/c or C57BL/6 mice after aerosol exposure to B . pseudomallei (A) HBPUB10134a, (B) MSHR5855, or (C) 1106a. Blood was drawn from mice (N = 5 for each time point) after exposure to B . pseudomallei strains when possible after 1, 2, 3, 7, 10, 14, 21, 28, and 60 days. ELISA was performed on the sera at least once in triplicate for each group of BALB/c (blue bars) and C57BL/6 (orange bars) mice and IgG titer reported as the geometric means with the standard error of the mean depicted. Due to their susceptibility not enough BALB/c mice survived to end of study, to determine the antibody levels, whereas C57BL/6 exposed mice survived until the end of the study (60 days). Significant differences in the IgG levels between BALB/c and C57BL/6 mice at specific time points after exposure is shown above the results.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: The IgG antibody response in BALB/c or C57BL/6 mice after aerosol exposure to B . pseudomallei (A) HBPUB10134a, (B) MSHR5855, or (C) 1106a. Blood was drawn from mice (N = 5 for each time point) after exposure to B . pseudomallei strains when possible after 1, 2, 3, 7, 10, 14, 21, 28, and 60 days. ELISA was performed on the sera at least once in triplicate for each group of BALB/c (blue bars) and C57BL/6 (orange bars) mice and IgG titer reported as the geometric means with the standard error of the mean depicted. Due to their susceptibility not enough BALB/c mice survived to end of study, to determine the antibody levels, whereas C57BL/6 exposed mice survived until the end of the study (60 days). Significant differences in the IgG levels between BALB/c and C57BL/6 mice at specific time points after exposure is shown above the results.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Plot of dose response curves for each B . pseudomallei clinical isolate in (A) BALB/c mice and (B) C57BL/6 mice.

    Journal: PLoS ONE

    Article Title: Disease progression in mice exposed to low-doses of aerosolized clinical isolates of Burkholderia pseudomallei

    doi: 10.1371/journal.pone.0208277

    Figure Lengend Snippet: Plot of dose response curves for each B . pseudomallei clinical isolate in (A) BALB/c mice and (B) C57BL/6 mice.

    Article Snippet: IL-1α was also differentially expressed between the BALB/c and C57BL/6 mice, with higher fold-change in the spleens of BALB/c mice, but higher fold-change on days one and two in the lungs of C57BL/6 mice.

    Techniques: Mouse Assay

    Changes in the amount of cytokines/chemokines in sera from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei K96243. The amount of cytokines/chemokines present in sera ( S7 Table ) was determined. For changes in cytokine/chemokine levels in sera from BALB/c mice (A) , we show changes in levels up to 22 days post exposure because there were no survivors after that period. We also show changes in cytokine/chemokine levels in sera for C57BL/6 mice (B) up to 22 days for comparison and not many significant changes occurred after 22 days in sera from C57BL/6 mice. For each mouse strain N = 5 at each time point. Fold-changes in cytokines/chemokines were determined by dividing the amount (pg/ml) present in sera of exposed mice ( S7 Table ) by the mount present in normal, naïve mice, where N = 10 for BALB/c and N = 4 for C57BL/6 mice. For C57BL/6 mice fold-change for MIG was not shown because it was very high (235-fold), and it would make obscure the changes in the levels of the other cytokines/chemokines at the same time.

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Changes in the amount of cytokines/chemokines in sera from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei K96243. The amount of cytokines/chemokines present in sera ( S7 Table ) was determined. For changes in cytokine/chemokine levels in sera from BALB/c mice (A) , we show changes in levels up to 22 days post exposure because there were no survivors after that period. We also show changes in cytokine/chemokine levels in sera for C57BL/6 mice (B) up to 22 days for comparison and not many significant changes occurred after 22 days in sera from C57BL/6 mice. For each mouse strain N = 5 at each time point. Fold-changes in cytokines/chemokines were determined by dividing the amount (pg/ml) present in sera of exposed mice ( S7 Table ) by the mount present in normal, naïve mice, where N = 10 for BALB/c and N = 4 for C57BL/6 mice. For C57BL/6 mice fold-change for MIG was not shown because it was very high (235-fold), and it would make obscure the changes in the levels of the other cytokines/chemokines at the same time.

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Mouse Assay

    Histopathology observed in mice with rear leg clinical signs assocaited with IP challenge with B . pseudomallei K96243. (A) C57BL/6 euthanized on day 22 post-infection showing clinical signs in the hind-end and tail. Tail, transverse section: Multiple pyogranulomas partially effacing vertebral body and associated soft tissues. H E, 20X. (B) BALB/c mouse euthanized on day 25 post-infection with rear-leg paralysis and labored breathing. Lumbar spine, longitudinal section: Pyogranulomatous inflammation partially effacing vertebral body and associated soft tissues. P = pyogranuloma; M = marrow cavity; V = vertebral body; H E, 40X.

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Histopathology observed in mice with rear leg clinical signs assocaited with IP challenge with B . pseudomallei K96243. (A) C57BL/6 euthanized on day 22 post-infection showing clinical signs in the hind-end and tail. Tail, transverse section: Multiple pyogranulomas partially effacing vertebral body and associated soft tissues. H E, 20X. (B) BALB/c mouse euthanized on day 25 post-infection with rear-leg paralysis and labored breathing. Lumbar spine, longitudinal section: Pyogranulomatous inflammation partially effacing vertebral body and associated soft tissues. P = pyogranuloma; M = marrow cavity; V = vertebral body; H E, 40X.

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Histopathology, Mouse Assay, Infection

    Bacterial burden determined in mice exposed to aerosolized B . pseudomallei K96243. CFU/g for spleen (A) , lungs (B) , liver (C) and CFU/ml for blood (D) are depicted. The geometric mean for each group is indicated. BALB/C mice are depicted with open circles and C57BL/6 mice are depicted with filled squares. N = 5 at each time point through day 22 for both mouse strains. The surviving BALB/c mice after day 22 were used to perform histopathological analyses. N = 12 for surviving C57BL/6 mice on day 91.

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Bacterial burden determined in mice exposed to aerosolized B . pseudomallei K96243. CFU/g for spleen (A) , lungs (B) , liver (C) and CFU/ml for blood (D) are depicted. The geometric mean for each group is indicated. BALB/C mice are depicted with open circles and C57BL/6 mice are depicted with filled squares. N = 5 at each time point through day 22 for both mouse strains. The surviving BALB/c mice after day 22 were used to perform histopathological analyses. N = 12 for surviving C57BL/6 mice on day 91.

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Mouse Assay

    Changes in the amount of cytokines/chemokines in spleen extracts from BALB/c and C57BL/6 mice after IP infection with B . pseudomallei K96243. The amount of cytokines/chemokines present in spleen extracts ( S4 Table ) was determined. Only fold-changes in ten of the cytokines/chemokines are shown for (A) BALB/c and (B) C57BL/6 because they showed the most changes from normal levels after infection or were known to be important for host immunity, such as TNF-α. For each time point, N = 5 for BALB/c and C57BL/6 mice. Fold-changes in cytokines/chemokines was determined by dividing the amount (pg/ml) present in the spleen extract by the amount present in normal, naïve mice, where N = 10 for BALB/c and N = 4 for C57BL/6 mice.

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Changes in the amount of cytokines/chemokines in spleen extracts from BALB/c and C57BL/6 mice after IP infection with B . pseudomallei K96243. The amount of cytokines/chemokines present in spleen extracts ( S4 Table ) was determined. Only fold-changes in ten of the cytokines/chemokines are shown for (A) BALB/c and (B) C57BL/6 because they showed the most changes from normal levels after infection or were known to be important for host immunity, such as TNF-α. For each time point, N = 5 for BALB/c and C57BL/6 mice. Fold-changes in cytokines/chemokines was determined by dividing the amount (pg/ml) present in the spleen extract by the amount present in normal, naïve mice, where N = 10 for BALB/c and N = 4 for C57BL/6 mice.

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Mouse Assay, Infection

    Cellular changes in spleens occurring in BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei K96243. Spleen homogenates were prepared from infected (A) BALB/c and (B) C57BL/6 mice over time, and the percent of each cell type examined was determined. After 22 days there were no BALB/c mice survivors. For each mouse strain N = 5 at each time point. The fold-change for each cell type was determined by dividing the percent of the cell type at each time point ( S6 Table ) by the percent of the cell type present in normal, naïve mice, where N = 10 for BALB/c and N = 4 for C57BL/6 mice. Significant changes in % cell type ( S6 Table ) are shown above the inflammatory cells (macrophages/monocytes, NK cells, and granulocytes) only because they showed the most predominate changes through out the study. Significant levels compared to the naïve, control mice: † P

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Cellular changes in spleens occurring in BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei K96243. Spleen homogenates were prepared from infected (A) BALB/c and (B) C57BL/6 mice over time, and the percent of each cell type examined was determined. After 22 days there were no BALB/c mice survivors. For each mouse strain N = 5 at each time point. The fold-change for each cell type was determined by dividing the percent of the cell type at each time point ( S6 Table ) by the percent of the cell type present in normal, naïve mice, where N = 10 for BALB/c and N = 4 for C57BL/6 mice. Significant changes in % cell type ( S6 Table ) are shown above the inflammatory cells (macrophages/monocytes, NK cells, and granulocytes) only because they showed the most predominate changes through out the study. Significant levels compared to the naïve, control mice: † P

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Mouse Assay, Infection

    Cellular changes in spleens occurring in BALB/c and C57BL/6 mice after IP infection with B . pseudomallei K96243. Spleen homogenates were prepared from (A) BALB/c and (B) C57BL/6 mice over time after infection, and the percent of each cell type examined was determined. For each mouse strain, N = 5 at each time point. The fold-change for each cell type was determined by dividing the percent of the cell type at each time point ( S2 Table ) by the percent of the cell type present in normal, naïve mice, where N = 10 for BALB/c and N = 4 for C57BL/6 mice. Significant changes in % cell type ( S2 Table ) are shown above the inflammatory cells (macrophages/monocytes, NK cells, and granulocytes) only because they showed the most predominate changes throughout the study. Significant levels compared to the naïve, control mice: ¶ P

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Cellular changes in spleens occurring in BALB/c and C57BL/6 mice after IP infection with B . pseudomallei K96243. Spleen homogenates were prepared from (A) BALB/c and (B) C57BL/6 mice over time after infection, and the percent of each cell type examined was determined. For each mouse strain, N = 5 at each time point. The fold-change for each cell type was determined by dividing the percent of the cell type at each time point ( S2 Table ) by the percent of the cell type present in normal, naïve mice, where N = 10 for BALB/c and N = 4 for C57BL/6 mice. Significant changes in % cell type ( S2 Table ) are shown above the inflammatory cells (macrophages/monocytes, NK cells, and granulocytes) only because they showed the most predominate changes throughout the study. Significant levels compared to the naïve, control mice: ¶ P

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Mouse Assay, Infection

    Histopathology observed in mice following IP challenge with B . pseudomallei K96243. (A) C57BL/6 mouse euthanized on day 2 post-infection Liver: Random foci of neutrophilic inflammation with individual hepatocyte necrosis/apoptosis (arrow). I = inflammation; H E, 400X. (B) BALB/c mouse euthanized on day 20 post-infection with rear-leg paralysis. Spleen: Multiple pyogranulomas effacing red and white pulp; H E, 40X. (C) C57BL/6 mouse euthanized on day 22 post-infection. Femoral bone marrow: Myeloid hyperplasia with predominance of neutrophils. H E, 400X. P = pyogranuloma; R = red pulp; W = white pulp;

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Histopathology observed in mice following IP challenge with B . pseudomallei K96243. (A) C57BL/6 mouse euthanized on day 2 post-infection Liver: Random foci of neutrophilic inflammation with individual hepatocyte necrosis/apoptosis (arrow). I = inflammation; H E, 400X. (B) BALB/c mouse euthanized on day 20 post-infection with rear-leg paralysis. Spleen: Multiple pyogranulomas effacing red and white pulp; H E, 40X. (C) C57BL/6 mouse euthanized on day 22 post-infection. Femoral bone marrow: Myeloid hyperplasia with predominance of neutrophils. H E, 400X. P = pyogranuloma; R = red pulp; W = white pulp;

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Histopathology, Mouse Assay, Infection

    Bacterial burden determined in mice challenged with B . pseudomallei K96243 delivered via the IP route. CFU/g for spleen (A) , lungs (B) , liver (C) and CFU/ml for blood (D) are depicted. The geometric mean for each group is indicated by a horizontal bar. BALB/C mice are depicted with open circles and C57BL/6 mice are depicted with filled squares. N = 5 at each time point.

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Bacterial burden determined in mice challenged with B . pseudomallei K96243 delivered via the IP route. CFU/g for spleen (A) , lungs (B) , liver (C) and CFU/ml for blood (D) are depicted. The geometric mean for each group is indicated by a horizontal bar. BALB/C mice are depicted with open circles and C57BL/6 mice are depicted with filled squares. N = 5 at each time point.

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Mouse Assay

    Lung histopathology observed in mice following exposure to aerosolized B . pseudomallei K96243. (A) C57BL/6 mouse euthanized on day 2 post-infection. Lung: Multifocal random (embolic) suppurative pneumonia. H E 20X. (B) BALB/c mouse euthanized on day 4 post-infection. Lung: Suppurative inflammation and alveolar necrosis with numerous short bacilli (arrow). P = pyogranuloma; H E 600X. (C) BALB/c mouse euthanized on day 7 post-infection. Lung: Focally extensive pyogranuloma. P = pyogranuloma; H E 40X. (D) Lung: Periphery of pyogranuloma with MNGC macrophage (arrow) formation. H E 600X.

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Lung histopathology observed in mice following exposure to aerosolized B . pseudomallei K96243. (A) C57BL/6 mouse euthanized on day 2 post-infection. Lung: Multifocal random (embolic) suppurative pneumonia. H E 20X. (B) BALB/c mouse euthanized on day 4 post-infection. Lung: Suppurative inflammation and alveolar necrosis with numerous short bacilli (arrow). P = pyogranuloma; H E 600X. (C) BALB/c mouse euthanized on day 7 post-infection. Lung: Focally extensive pyogranuloma. P = pyogranuloma; H E 40X. (D) Lung: Periphery of pyogranuloma with MNGC macrophage (arrow) formation. H E 600X.

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Histopathology, Mouse Assay, Infection

    Changes in the amount of cytokines/chemokines in spleen extracts from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei K96243. The amount of cytokines/chemokines present in spleen extracts ( S8 Table ) was determined. For changes in cytokine/chemokine levels in spleen extracts from BALB/c mice (A) , we show changes in levels up to 22 days because there were no survivors after that period. We also show changes in cytokines/chemokine levels in spleen extracts for C57BL/6 mice (B) for comparison although they were determined up to day 91 ( S8 Table ). For each time point, N = 5 for BALB/c and C57BL/6 mice. Fold-changes in cytokines/chemokines was determined by dividing the amount ( S8 Table ) present in the spleen extract by the amount present in normal, naïve mice, where N = 10 for BALB/c and 4 for C57BL/6 mice.

    Journal: PLoS ONE

    Article Title: Characterization of pathogenesis of and immune response to Burkholderia pseudomallei K96243 using both inhalational and intraperitoneal infection models in BALB/c and C57BL/6 mice

    doi: 10.1371/journal.pone.0172627

    Figure Lengend Snippet: Changes in the amount of cytokines/chemokines in spleen extracts from BALB/c and C57BL/6 mice after aerosol exposure to B . pseudomallei K96243. The amount of cytokines/chemokines present in spleen extracts ( S8 Table ) was determined. For changes in cytokine/chemokine levels in spleen extracts from BALB/c mice (A) , we show changes in levels up to 22 days because there were no survivors after that period. We also show changes in cytokines/chemokine levels in spleen extracts for C57BL/6 mice (B) for comparison although they were determined up to day 91 ( S8 Table ). For each time point, N = 5 for BALB/c and C57BL/6 mice. Fold-changes in cytokines/chemokines was determined by dividing the amount ( S8 Table ) present in the spleen extract by the amount present in normal, naïve mice, where N = 10 for BALB/c and 4 for C57BL/6 mice.

    Article Snippet: Overall, the pattern of increase in the inflammatory cells (primarily granulocytes, followed by monocytes/macrophages and NK cells) in spleens from infected BALB/c mice was similar to that seen in infected spleens from C57BL/6 mice, except for the early influx in these cells at day 4 that we observed in infected spleens from C57BL/6 mice ( ).

    Techniques: Mouse Assay

    Organ fatty acid compositions in diet-induced obese C57BL/6 J mice fed the control, EPA or LCMUFA diet for 8 weeks. Fatty acid composition in liver ( a ) and epididymal adipose tissue ( b ) at the end of 8 weeks. Values are means ± SEM,  n  = 10. Significantly different mean values ( P

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Organ fatty acid compositions in diet-induced obese C57BL/6 J mice fed the control, EPA or LCMUFA diet for 8 weeks. Fatty acid composition in liver ( a ) and epididymal adipose tissue ( b ) at the end of 8 weeks. Values are means ± SEM, n  = 10. Significantly different mean values ( P

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay

    Gene expressions in adipose tissue of diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. mRNAs transcribed from insulin signaling–related genes ( a ) and pro-inflammatory genes ( b ) in epididymal adipose tissue at the end of 18 weeks. Values are means ± SEM,  n  = 10. * P

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Gene expressions in adipose tissue of diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. mRNAs transcribed from insulin signaling–related genes ( a ) and pro-inflammatory genes ( b ) in epididymal adipose tissue at the end of 18 weeks. Values are means ± SEM, n  = 10. * P

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay

    Lipid desaturation index in diet-induced obese C57BL/6 J mice. Ratio of 16:1/16:0 (left panels) and 18:1/18:0 (right panels) in epididymal WAT and liver at the end of 18 weeks in mice fed diets enriched in lard or saury oil ( a ) and at the end of 8 weeks in mice fed diets enriched in lard, EPA, or LCMUFA ( b ). Values are means ± SEM,  n  = 10. Significantly different mean values ( P

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Lipid desaturation index in diet-induced obese C57BL/6 J mice. Ratio of 16:1/16:0 (left panels) and 18:1/18:0 (right panels) in epididymal WAT and liver at the end of 18 weeks in mice fed diets enriched in lard or saury oil ( a ) and at the end of 8 weeks in mice fed diets enriched in lard, EPA, or LCMUFA ( b ). Values are means ± SEM, n  = 10. Significantly different mean values ( P

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay

    Adipocyte size in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Adipocyte size in epididymal adipose ( a ) and subcutaneous adipose ( b ) tissue at the end of 18 weeks. The left panels indicate the frequency distribution of adipocyte size, and the right panels indicate the mean size of adipocytes. Values are means ± SEM,  n  = 4. * P

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Adipocyte size in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Adipocyte size in epididymal adipose ( a ) and subcutaneous adipose ( b ) tissue at the end of 18 weeks. The left panels indicate the frequency distribution of adipocyte size, and the right panels indicate the mean size of adipocytes. Values are means ± SEM, n  = 4. * P

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay

    Plasma insulin concentrations in diet-induced obese C57BL/6 J mice. Plasma insulin concentrations at the end of 18 weeks in mice fed diets enriched in lard or saury oil ( a ) and at the end of 8 weeks in mice fed diets enriched in lard, EPA, or LCMUFA ( b ). Values are means ± SEM,  n  = 10. Significantly different mean values ( P

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Plasma insulin concentrations in diet-induced obese C57BL/6 J mice. Plasma insulin concentrations at the end of 18 weeks in mice fed diets enriched in lard or saury oil ( a ) and at the end of 8 weeks in mice fed diets enriched in lard, EPA, or LCMUFA ( b ). Values are means ± SEM, n  = 10. Significantly different mean values ( P

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay

    Organ LCMUFA compositions in diet-induced obese C57BL/6 J mice fed the control, EPA or LCMUFA diet for 8 weeks. Percentages of C20:1 n-9 ( a ), C20:1 n-11 ( b ), C22:1 n-9 ( c ) and C22:1 n-11 ( d ) in total lipids of WAT and liver at the end of 18 weeks. Values are means ± SEM,  n  = 10. Significantly different mean values (P 

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Organ LCMUFA compositions in diet-induced obese C57BL/6 J mice fed the control, EPA or LCMUFA diet for 8 weeks. Percentages of C20:1 n-9 ( a ), C20:1 n-11 ( b ), C22:1 n-9 ( c ) and C22:1 n-11 ( d ) in total lipids of WAT and liver at the end of 18 weeks. Values are means ± SEM, n  = 10. Significantly different mean values (P 

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay

    Liver lipid concentrations in diet-induced obese C57BL/6 J mice. Liver lipid concentrations at the end of 18 weeks in mice fed diets enriched in lard or saury oil ( a ) and at the end of 8 weeks in mice fed diets enriched in lard, EPA, or LCMUFA ( b ). Values are means ± SEM, n = 10. Significantly different mean values ( P

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Liver lipid concentrations in diet-induced obese C57BL/6 J mice. Liver lipid concentrations at the end of 18 weeks in mice fed diets enriched in lard or saury oil ( a ) and at the end of 8 weeks in mice fed diets enriched in lard, EPA, or LCMUFA ( b ). Values are means ± SEM, n = 10. Significantly different mean values ( P

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay

    Energy expenditure in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Oxygen consumption ( a ), respiratory quotient ( b ) and spontaneous locomotor activity ( c ) at baseline, 5, 12 and 18 weeks. Values are means ± SEM,  n  = 10

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Energy expenditure in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Oxygen consumption ( a ), respiratory quotient ( b ) and spontaneous locomotor activity ( c ) at baseline, 5, 12 and 18 weeks. Values are means ± SEM, n  = 10

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay, Activity Assay

    Organ and plasma fatty acid compositions in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Percentages of total SFA ( a ), total MUFA ( b ), total n-6 PUFA ( c ), total n-3 PUFA ( d ) in total lipids of WAT, liver, duodenum, muscle, and plasma at the end of 18 weeks. Values are means ± SEM,  n  = 10. * P

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Organ and plasma fatty acid compositions in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Percentages of total SFA ( a ), total MUFA ( b ), total n-6 PUFA ( c ), total n-3 PUFA ( d ) in total lipids of WAT, liver, duodenum, muscle, and plasma at the end of 18 weeks. Values are means ± SEM, n  = 10. * P

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay

    Organ and plasma levels of individual types of LCMUFA in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Percentages of C20:1 n-9 ( a ), C20:1 n-11 ( b ), C22:1 n-9 ( c ) and C22:1 n-11 ( d ) in total lipids of WAT, liver, duodenum, muscle, and plasma at the end of 18 weeks. Values are means ± SEM,  n  = 10. ** P

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Organ and plasma levels of individual types of LCMUFA in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Percentages of C20:1 n-9 ( a ), C20:1 n-11 ( b ), C22:1 n-9 ( c ) and C22:1 n-11 ( d ) in total lipids of WAT, liver, duodenum, muscle, and plasma at the end of 18 weeks. Values are means ± SEM, n  = 10. ** P

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay

    Organ and plasma levels of MUFA in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Percentages of palmitoleic acid ( a ), oleic acid ( b ) and LCMUFA ( c ) in total lipids of WAT, liver, duodenum, muscle, and plasma at the end of 18 weeks. Values are means ± SEM,  n  = 10. * P

    Journal: Lipids in Health and Disease

    Article Title: Long-term dietary supplementation with saury oil attenuates metabolic abnormalities in mice fed a high-fat diet: combined beneficial effect of omega-3 fatty acids and long-chain monounsaturated fatty acids

    doi: 10.1186/s12944-015-0161-8

    Figure Lengend Snippet: Organ and plasma levels of MUFA in diet-induced obese C57BL/6 J mice fed the control or saury oil diet for 18 weeks. Percentages of palmitoleic acid ( a ), oleic acid ( b ) and LCMUFA ( c ) in total lipids of WAT, liver, duodenum, muscle, and plasma at the end of 18 weeks. Values are means ± SEM, n  = 10. * P

    Article Snippet: In Experiment 2, 5-week-old male C57BL/6 J mice (30 total; Japan SLC, Inc.) were acclimatized for a 2-week period and then assigned to one of three dietary groups (n = 10 per group).

    Techniques: Mouse Assay