c43 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Millipore c43
    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) <t>C43</t> harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)
    C43, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43/product/Millipore
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    c43 - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    88
    Lucigen Corp c43
    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) <t>C43</t> harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)
    C43, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 88/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43/product/Lucigen Corp
    Average 88 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    c43 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    c43  (Abcam)
    95
    Abcam c43
    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) <t>C43</t> harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)
    C43, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43/product/Abcam
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    c43 - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    88
    Lucigen Corp c43 de3
    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) <t>C43</t> harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)
    C43 De3, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43 de3/product/Lucigen Corp
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    c43 de3 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    94
    Cambridge Bioscience c43 cells
    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) <t>C43</t> harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)
    C43 Cells, supplied by Cambridge Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43 cells/product/Cambridge Bioscience
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    c43 cells - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    94
    Cosmo Bio c43
    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) <t>C43</t> harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)
    C43, supplied by Cosmo Bio, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43/product/Cosmo Bio
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    c43 - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    88
    Thermo Fisher c43
    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) <t>C43</t> harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)
    C43, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43/product/Thermo Fisher
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    c43 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    93
    Millipore overexpress c43 de3 cells
    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) <t>C43</t> harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)
    Overexpress C43 De3 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/overexpress c43 de3 cells/product/Millipore
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    overexpress c43 de3 cells - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    99
    Millipore strain c43
    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) <t>C43</t> harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)
    Strain C43, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strain c43/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    strain c43 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    c43  (Amgen)
    88
    Amgen c43
    Schematic representation of native and chimeric human FPR1 and FPR2/ALX clones used in the study. A , FPR2/ALX sequence with amino acids different from human FPR1 highlighted in red. B , FPR1-FPR2/ALX clones used in this study. Each clone is named alphabetically and has distinct domains of FPR1 replaced with the indicated FPR2/ALX amino acid sequences. C , typical Ca 2+ flux responses evoked in FPR2/ALX cells upon treatment with AnxA1 or <t>C43</t> ( left panel ) and with SAA ( right panel ). Data (means ± S.E. of five distinct experiments done in triplicate) are reported as percent ionomycin (1 μ m ) response.
    C43, supplied by Amgen, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43/product/Amgen
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    c43 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    89
    Roche c43 de3
    Schematic representation of native and chimeric human FPR1 and FPR2/ALX clones used in the study. A , FPR2/ALX sequence with amino acids different from human FPR1 highlighted in red. B , FPR1-FPR2/ALX clones used in this study. Each clone is named alphabetically and has distinct domains of FPR1 replaced with the indicated FPR2/ALX amino acid sequences. C , typical Ca 2+ flux responses evoked in FPR2/ALX cells upon treatment with AnxA1 or <t>C43</t> ( left panel ) and with SAA ( right panel ). Data (means ± S.E. of five distinct experiments done in triplicate) are reported as percent ionomycin (1 μ m ) response.
    C43 De3, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43 de3/product/Roche
    Average 89 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    c43 de3 - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    91
    Millipore overexpress c43 de3 plyss
    Expression screening in E. coli . Expression screening was carried out in 96-well microtiter plates to identify high producers for three different target proteins. The promoter, protein fusion partner and E. coli strain were varied. a IMPI, b BR021, c AFP. Rosetta-gami 2 Rosetta-gami 2 (DE3) <t>pLysS,</t> Origami Origami 2, SHuffle SHuffle T7 Express lysY, BL21 BL21 (DE3), C41 OverExpress C41 (DE3) pLysS, <t>C43</t> OverExpress C43 (DE3) pLysS
    Overexpress C43 De3 Plyss, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/overexpress c43 de3 plyss/product/Millipore
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    overexpress c43 de3 plyss - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    90
    Agilent technologies c43 de3
    Expression screening in E. coli . Expression screening was carried out in 96-well microtiter plates to identify high producers for three different target proteins. The promoter, protein fusion partner and E. coli strain were varied. a IMPI, b BR021, c AFP. Rosetta-gami 2 Rosetta-gami 2 (DE3) <t>pLysS,</t> Origami Origami 2, SHuffle SHuffle T7 Express lysY, BL21 BL21 (DE3), C41 OverExpress C41 (DE3) pLysS, <t>C43</t> OverExpress C43 (DE3) pLysS
    C43 De3, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c43 de3/product/Agilent technologies
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    c43 de3 - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    92
    MTS Systems Corporation mts c43 304
    Expression screening in E. coli . Expression screening was carried out in 96-well microtiter plates to identify high producers for three different target proteins. The promoter, protein fusion partner and E. coli strain were varied. a IMPI, b BR021, c AFP. Rosetta-gami 2 Rosetta-gami 2 (DE3) <t>pLysS,</t> Origami Origami 2, SHuffle SHuffle T7 Express lysY, BL21 BL21 (DE3), C41 OverExpress C41 (DE3) pLysS, <t>C43</t> OverExpress C43 (DE3) pLysS
    Mts C43 304, supplied by MTS Systems Corporation, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mts c43 304/product/MTS Systems Corporation
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mts c43 304 - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Abcam anti cytokeratin 8 antibody c 43
    Expression screening in E. coli . Expression screening was carried out in 96-well microtiter plates to identify high producers for three different target proteins. The promoter, protein fusion partner and E. coli strain were varied. a IMPI, b BR021, c AFP. Rosetta-gami 2 Rosetta-gami 2 (DE3) <t>pLysS,</t> Origami Origami 2, SHuffle SHuffle T7 Express lysY, BL21 BL21 (DE3), C41 OverExpress C41 (DE3) pLysS, <t>C43</t> OverExpress C43 (DE3) pLysS
    Anti Cytokeratin 8 Antibody C 43, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cytokeratin 8 antibody c 43/product/Abcam
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti cytokeratin 8 antibody c 43 - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    88
    Lucigen Corp escherichia coli strain c43
    Expression screening in E. coli . Expression screening was carried out in 96-well microtiter plates to identify high producers for three different target proteins. The promoter, protein fusion partner and E. coli strain were varied. a IMPI, b BR021, c AFP. Rosetta-gami 2 Rosetta-gami 2 (DE3) <t>pLysS,</t> Origami Origami 2, SHuffle SHuffle T7 Express lysY, BL21 BL21 (DE3), C41 OverExpress C41 (DE3) pLysS, <t>C43</t> OverExpress C43 (DE3) pLysS
    Escherichia Coli Strain C43, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 88/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain c43/product/Lucigen Corp
    Average 88 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain c43 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    88
    Thermo Fisher e coli strain c43
    Expression screening in E. coli . Expression screening was carried out in 96-well microtiter plates to identify high producers for three different target proteins. The promoter, protein fusion partner and E. coli strain were varied. a IMPI, b BR021, c AFP. Rosetta-gami 2 Rosetta-gami 2 (DE3) <t>pLysS,</t> Origami Origami 2, SHuffle SHuffle T7 Express lysY, BL21 BL21 (DE3), C41 OverExpress C41 (DE3) pLysS, <t>C43</t> OverExpress C43 (DE3) pLysS
    E Coli Strain C43, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain c43/product/Thermo Fisher
    Average 88 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    e coli strain c43 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) C43 harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)

    Journal: bioRxiv

    Article Title: Cyclic oligoadenylate signalling mediates Mycobacterium tuberculosis CRISPR defence

    doi: 10.1101/667758

    Figure Lengend Snippet: Plasmid immunity assays of Mtb Csm in heterologous host E. coli . ( A ) C43 harbouring pCsm1-6 and pCRISPR are transformed with pRAT (control plasmid) or with pRAT-target (plasmid with pUC19 MCS target). Target transcription was arabinose-inducible. In the absence of arabinose, the transformation efficiency was independent of the presence or absence of target; whereas when target transcription was induced, a 2-log reduction of transformation efficiency was observed for the target plasmid compared to the control plasmid. *** p-value (Welch two sample test, two tailed)

    Article Snippet: Mtb Csm6 and T. sulfidiphilus Csx1 Mtb Csm6 and TsuCsx1 were expressed in C43 cells.

    Techniques: Plasmid Preparation, Transformation Assay, Two Tailed Test

    Re-programming the Mtb Csm system for cA 4 -responsive immunity. ( A ) In vitro activity of TsuCsx1. The reaction contained 0.5 µM TsuCsx1 dimer, 100 nM 5’- 32 P-labeled RNA A1, 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH 7.5 and was conducted at 35 °C for 1, 5, 15, 30 min. Activators were added as indicated; Csm-derived cOAs were from a 2 h reaction, otherwise as described for Figure 3 . TsuCsx1 was activated by cA 4 but not cA 6 , and Csm-derived cOAs are able to induce Csx1 ribonuclease activity in vitro . ( B ) Plasmid immunity assay using pUC19 lacZα -targeting Csm effector complex in E. coli C43 (pCsm1-5_Csm6/tsuCsx1 and pCRISPR, Figure S4 ). The ribonuclease was either the cognate Csm6 or TsuCsx1. Cells were transformed with pRAT (control plasmid) or pRAT-Target (target plasmid) and 10-fold serial dilutions were plated on selective plates containing arabinose for induction of target transcription. TsuCsx1 confers the same level of plasmid immunity as the cognate Mtb Csm6.

    Journal: bioRxiv

    Article Title: Cyclic oligoadenylate signalling mediates Mycobacterium tuberculosis CRISPR defence

    doi: 10.1101/667758

    Figure Lengend Snippet: Re-programming the Mtb Csm system for cA 4 -responsive immunity. ( A ) In vitro activity of TsuCsx1. The reaction contained 0.5 µM TsuCsx1 dimer, 100 nM 5’- 32 P-labeled RNA A1, 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH 7.5 and was conducted at 35 °C for 1, 5, 15, 30 min. Activators were added as indicated; Csm-derived cOAs were from a 2 h reaction, otherwise as described for Figure 3 . TsuCsx1 was activated by cA 4 but not cA 6 , and Csm-derived cOAs are able to induce Csx1 ribonuclease activity in vitro . ( B ) Plasmid immunity assay using pUC19 lacZα -targeting Csm effector complex in E. coli C43 (pCsm1-5_Csm6/tsuCsx1 and pCRISPR, Figure S4 ). The ribonuclease was either the cognate Csm6 or TsuCsx1. Cells were transformed with pRAT (control plasmid) or pRAT-Target (target plasmid) and 10-fold serial dilutions were plated on selective plates containing arabinose for induction of target transcription. TsuCsx1 confers the same level of plasmid immunity as the cognate Mtb Csm6.

    Article Snippet: Mtb Csm6 and T. sulfidiphilus Csx1 Mtb Csm6 and TsuCsx1 were expressed in C43 cells.

    Techniques: In Vitro, Activity Assay, Labeling, Derivative Assay, Plasmid Preparation, Transformation Assay

    Schematic representation of native and chimeric human FPR1 and FPR2/ALX clones used in the study. A , FPR2/ALX sequence with amino acids different from human FPR1 highlighted in red. B , FPR1-FPR2/ALX clones used in this study. Each clone is named alphabetically and has distinct domains of FPR1 replaced with the indicated FPR2/ALX amino acid sequences. C , typical Ca 2+ flux responses evoked in FPR2/ALX cells upon treatment with AnxA1 or C43 ( left panel ) and with SAA ( right panel ). Data (means ± S.E. of five distinct experiments done in triplicate) are reported as percent ionomycin (1 μ m ) response.

    Journal: The Journal of Biological Chemistry

    Article Title: Annexin A1 Interaction with the FPR2/ALX Receptor

    doi: 10.1074/jbc.M112.377101

    Figure Lengend Snippet: Schematic representation of native and chimeric human FPR1 and FPR2/ALX clones used in the study. A , FPR2/ALX sequence with amino acids different from human FPR1 highlighted in red. B , FPR1-FPR2/ALX clones used in this study. Each clone is named alphabetically and has distinct domains of FPR1 replaced with the indicated FPR2/ALX amino acid sequences. C , typical Ca 2+ flux responses evoked in FPR2/ALX cells upon treatment with AnxA1 or C43 ( left panel ) and with SAA ( right panel ). Data (means ± S.E. of five distinct experiments done in triplicate) are reported as percent ionomycin (1 μ m ) response.

    Article Snippet: SAA was purchased from PeproTech Laboratories (London, United Kingdom), and C43 was a generous gift from Amgen (Thousands Oaks, CA).

    Techniques: Clone Assay, Sequencing

    Clone-specific calcium mobilization induced by AnxA1, SAA, and C43. HEK293 cells expressing native and chimeric clones were treated with AnxA1 (10 n m ; A ), SAA (0.1 μ m ; B ), or C43 (1 μ m ; C ) (concentrations selected from Fig. 1 ). Dashed lines indicate the degree of response produced with control CMV empty plasmid-transfected HEK293 cells in the presence of the respective ligand. Data (means ± S.E. of more than three distinct experiments done in triplicate) are reported as percent ionomycin (1 μ m ) response. Asterisks indicate similarity in responses between each clone (as indicated) and native FPR2/ALX-transfected cells. FPR1-FPR2/ALX clones are represented, with the part of the FPR2/ALX receptor insert ( red ) in the FPR1 structure ( black ) ( D ).

    Journal: The Journal of Biological Chemistry

    Article Title: Annexin A1 Interaction with the FPR2/ALX Receptor

    doi: 10.1074/jbc.M112.377101

    Figure Lengend Snippet: Clone-specific calcium mobilization induced by AnxA1, SAA, and C43. HEK293 cells expressing native and chimeric clones were treated with AnxA1 (10 n m ; A ), SAA (0.1 μ m ; B ), or C43 (1 μ m ; C ) (concentrations selected from Fig. 1 ). Dashed lines indicate the degree of response produced with control CMV empty plasmid-transfected HEK293 cells in the presence of the respective ligand. Data (means ± S.E. of more than three distinct experiments done in triplicate) are reported as percent ionomycin (1 μ m ) response. Asterisks indicate similarity in responses between each clone (as indicated) and native FPR2/ALX-transfected cells. FPR1-FPR2/ALX clones are represented, with the part of the FPR2/ALX receptor insert ( red ) in the FPR1 structure ( black ) ( D ).

    Article Snippet: SAA was purchased from PeproTech Laboratories (London, United Kingdom), and C43 was a generous gift from Amgen (Thousands Oaks, CA).

    Techniques: Expressing, Clone Assay, Produced, Plasmid Preparation, Transfection

    Schematic summary for agonist binding of FPR2/ALX. A , AnxA1 requires the N terminus and/or extracellular loop II to induce receptor activation. SAA interacts with extracellular loops I and II for FPR2/ALX, whereas the small molecule C43 penetrates into the extracellular loop I and transmembrane regions for full agonism. The reported binding site for lipoxin A 4 ( LXA 4 ) is also indicated as reported ( 5 ). B , summary of AnxA1-evoked post-FPR2/ALX signaling. The FPR2/ALX N-terminal domain conveys AnxA1 signaling through calcium mobilization and ERK phosphorylation, leading to JAG1 up-regulation and JAM3 down-regulation. Extracellular loop II is more complex in its downstream AnxA1-evoked events. It participates in AnxA1-mediated JAM3 down-regulation through calcium flux and ERK phosphorylation, and it may involve unknown pathways for SGGP2 induction.

    Journal: The Journal of Biological Chemistry

    Article Title: Annexin A1 Interaction with the FPR2/ALX Receptor

    doi: 10.1074/jbc.M112.377101

    Figure Lengend Snippet: Schematic summary for agonist binding of FPR2/ALX. A , AnxA1 requires the N terminus and/or extracellular loop II to induce receptor activation. SAA interacts with extracellular loops I and II for FPR2/ALX, whereas the small molecule C43 penetrates into the extracellular loop I and transmembrane regions for full agonism. The reported binding site for lipoxin A 4 ( LXA 4 ) is also indicated as reported ( 5 ). B , summary of AnxA1-evoked post-FPR2/ALX signaling. The FPR2/ALX N-terminal domain conveys AnxA1 signaling through calcium mobilization and ERK phosphorylation, leading to JAG1 up-regulation and JAM3 down-regulation. Extracellular loop II is more complex in its downstream AnxA1-evoked events. It participates in AnxA1-mediated JAM3 down-regulation through calcium flux and ERK phosphorylation, and it may involve unknown pathways for SGGP2 induction.

    Article Snippet: SAA was purchased from PeproTech Laboratories (London, United Kingdom), and C43 was a generous gift from Amgen (Thousands Oaks, CA).

    Techniques: Binding Assay, Activation Assay

    FPR2/ALX desensitization in human neutrophils upon agonist stimulations. Freshly isolated human neutrophils were treated with vehicle, AnxA1 (10 n m ), SAA (0.1 μ m ), or C43 (1 μ m ) for 5 min prior to the addition of AnxA1 (10 n m ; A ) or SAA (0.1 μ m ; B ). Data (means ± S.E. of more than three distinct experiments done in triplicate) are reported as percent ionomycin (1 μ m ) response. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Annexin A1 Interaction with the FPR2/ALX Receptor

    doi: 10.1074/jbc.M112.377101

    Figure Lengend Snippet: FPR2/ALX desensitization in human neutrophils upon agonist stimulations. Freshly isolated human neutrophils were treated with vehicle, AnxA1 (10 n m ), SAA (0.1 μ m ), or C43 (1 μ m ) for 5 min prior to the addition of AnxA1 (10 n m ; A ) or SAA (0.1 μ m ; B ). Data (means ± S.E. of more than three distinct experiments done in triplicate) are reported as percent ionomycin (1 μ m ) response. *, p

    Article Snippet: SAA was purchased from PeproTech Laboratories (London, United Kingdom), and C43 was a generous gift from Amgen (Thousands Oaks, CA).

    Techniques: Isolation

    Clone-specific ERK1/2 phosphorylation induced by AnxA1, SAA, and C43. HEK293 cells expressing native and chimeric clones were treated with AnxA1 (10 n m ; A ), SAA (0.1 μ m ; B ), or C43 (1 μ m ; C ) (concentrations selected from Fig. 1 ) for 5 or 10 min as indicated, and the phosphorylation of ERK1/2 was monitored by Western blot analysis. Representative blots from three or more experiments with distinct cell preparations are shown. tERK , total ERK.

    Journal: The Journal of Biological Chemistry

    Article Title: Annexin A1 Interaction with the FPR2/ALX Receptor

    doi: 10.1074/jbc.M112.377101

    Figure Lengend Snippet: Clone-specific ERK1/2 phosphorylation induced by AnxA1, SAA, and C43. HEK293 cells expressing native and chimeric clones were treated with AnxA1 (10 n m ; A ), SAA (0.1 μ m ; B ), or C43 (1 μ m ; C ) (concentrations selected from Fig. 1 ) for 5 or 10 min as indicated, and the phosphorylation of ERK1/2 was monitored by Western blot analysis. Representative blots from three or more experiments with distinct cell preparations are shown. tERK , total ERK.

    Article Snippet: SAA was purchased from PeproTech Laboratories (London, United Kingdom), and C43 was a generous gift from Amgen (Thousands Oaks, CA).

    Techniques: Expressing, Clone Assay, Western Blot

    Expression screening in E. coli . Expression screening was carried out in 96-well microtiter plates to identify high producers for three different target proteins. The promoter, protein fusion partner and E. coli strain were varied. a IMPI, b BR021, c AFP. Rosetta-gami 2 Rosetta-gami 2 (DE3) pLysS, Origami Origami 2, SHuffle SHuffle T7 Express lysY, BL21 BL21 (DE3), C41 OverExpress C41 (DE3) pLysS, C43 OverExpress C43 (DE3) pLysS

    Journal: Microbial Cell Factories

    Article Title: A high-throughput expression screening platform to optimize the production of antimicrobial peptides

    doi: 10.1186/s12934-017-0637-5

    Figure Lengend Snippet: Expression screening in E. coli . Expression screening was carried out in 96-well microtiter plates to identify high producers for three different target proteins. The promoter, protein fusion partner and E. coli strain were varied. a IMPI, b BR021, c AFP. Rosetta-gami 2 Rosetta-gami 2 (DE3) pLysS, Origami Origami 2, SHuffle SHuffle T7 Express lysY, BL21 BL21 (DE3), C41 OverExpress C41 (DE3) pLysS, C43 OverExpress C43 (DE3) pLysS

    Article Snippet: Transformation of E. coli expression strains Six different E. coli strains were used for expression screening: Rosetta-gami 2 (DE3) pLysS (Merck Millipore), Origami 2 (Merck Millipore), BL21 (DE3) (NEB), SHuffle T7 Express lysY (NEB), OverExpress C41 (DE3) pLysS (Sigma-Aldrich), OverExpress C43 (DE3) pLysS (Sigma-Aldrich).

    Techniques: Expressing