c2c12 myoblast cell line Search Results


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  • 99
    ATCC c2c12 subclone
    C2c12 Subclone, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1 article reviews
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    97
    ATCC c2c12 cell culture c2c12 myoblasts
    C2c12 Cell Culture C2c12 Myoblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c2c12 cell culture c2c12 myoblasts/product/ATCC
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    c2c12 cell culture c2c12 myoblasts - by Bioz Stars, 2024-06
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    96
    ATCC mouse muscle cell lines
    Mouse Muscle Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher c2c12 myoblasts
    C2c12 Myoblasts, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore c2c12 myoblasts
    A. 3T3-L1 adipocytes were differentiated in IS (left panel) and IR (right panel) conditions and treated for the last 24hrs of differentiation with different PCB126 concentrations. B. Levels of mitochondrial complexes (complexes II and III and ATPase) in 3T3-L1 adipocytes differentiated in IR conditions and exposed for 24hrs to different concentrations of PCB126. Right panel: quantification by density analysis, left panel: representative western blots. α-tubulin was used a loading control. n=3 independent experiments. C. Differentiated <t>C2C12</t> myotubes were exposed for the last 24hrs of differentiation to the CM of 3T3-L1 adipocytes exposed to different PCB126 concentrations in IS (left panel) or IR conditions (right panel). D. Differentiated C2C12 myotubes were directly exposed for the last 24hrs of differentiation to different PCB126 concentrations in IS (left panel) or IR conditions (right panel). A, C, and D. Oxygen consumption rates (OCR) were measured with a Seahorse analyzer (Agilent). OCR were first measured in resting conditions, and cells were treated subsequently with 600 ng/mL oligomycin, 1 μM carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP), and 2 μM (for 3T3-L1) or 4 μM antimycin A (for C2C12) to determine OCR due to proton leak, maximal, and non-mitochondrial respiration, respectively. n=4 independent experiments, each independent experiment was done in 5 replicates. A-D. Data are presented relative to the vehicle as mean ±SEM. *: P <0.05, **: P <0.01 compared to 0 nM.
    C2c12 Myoblasts, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c2c12 myoblasts/product/Millipore
    Average 86 stars, based on 1 article reviews
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    c2c12 myoblasts - by Bioz Stars, 2024-06
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    Image Search Results


    A. 3T3-L1 adipocytes were differentiated in IS (left panel) and IR (right panel) conditions and treated for the last 24hrs of differentiation with different PCB126 concentrations. B. Levels of mitochondrial complexes (complexes II and III and ATPase) in 3T3-L1 adipocytes differentiated in IR conditions and exposed for 24hrs to different concentrations of PCB126. Right panel: quantification by density analysis, left panel: representative western blots. α-tubulin was used a loading control. n=3 independent experiments. C. Differentiated C2C12 myotubes were exposed for the last 24hrs of differentiation to the CM of 3T3-L1 adipocytes exposed to different PCB126 concentrations in IS (left panel) or IR conditions (right panel). D. Differentiated C2C12 myotubes were directly exposed for the last 24hrs of differentiation to different PCB126 concentrations in IS (left panel) or IR conditions (right panel). A, C, and D. Oxygen consumption rates (OCR) were measured with a Seahorse analyzer (Agilent). OCR were first measured in resting conditions, and cells were treated subsequently with 600 ng/mL oligomycin, 1 μM carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP), and 2 μM (for 3T3-L1) or 4 μM antimycin A (for C2C12) to determine OCR due to proton leak, maximal, and non-mitochondrial respiration, respectively. n=4 independent experiments, each independent experiment was done in 5 replicates. A-D. Data are presented relative to the vehicle as mean ±SEM. *: P <0.05, **: P <0.01 compared to 0 nM.

    Journal: bioRxiv

    Article Title: PCB126-mediated effects on adipocyte energy metabolism and adipokine secretion may result in abnormal glucose uptake in muscle cells

    doi: 10.1101/2020.07.07.192245

    Figure Lengend Snippet: A. 3T3-L1 adipocytes were differentiated in IS (left panel) and IR (right panel) conditions and treated for the last 24hrs of differentiation with different PCB126 concentrations. B. Levels of mitochondrial complexes (complexes II and III and ATPase) in 3T3-L1 adipocytes differentiated in IR conditions and exposed for 24hrs to different concentrations of PCB126. Right panel: quantification by density analysis, left panel: representative western blots. α-tubulin was used a loading control. n=3 independent experiments. C. Differentiated C2C12 myotubes were exposed for the last 24hrs of differentiation to the CM of 3T3-L1 adipocytes exposed to different PCB126 concentrations in IS (left panel) or IR conditions (right panel). D. Differentiated C2C12 myotubes were directly exposed for the last 24hrs of differentiation to different PCB126 concentrations in IS (left panel) or IR conditions (right panel). A, C, and D. Oxygen consumption rates (OCR) were measured with a Seahorse analyzer (Agilent). OCR were first measured in resting conditions, and cells were treated subsequently with 600 ng/mL oligomycin, 1 μM carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP), and 2 μM (for 3T3-L1) or 4 μM antimycin A (for C2C12) to determine OCR due to proton leak, maximal, and non-mitochondrial respiration, respectively. n=4 independent experiments, each independent experiment was done in 5 replicates. A-D. Data are presented relative to the vehicle as mean ±SEM. *: P <0.05, **: P <0.01 compared to 0 nM.

    Article Snippet: C2C12 myoblasts (Sigma-Aldrich) were grown in low glucose DMEM, 10% fetal bovine serum (FBS, Wisent) and 1X AA.

    Techniques: Western Blot

    A-B. 3T3-L1 adipocytes were differentiated in IS (A) and IR (B) conditions and treated for the last 24hrs of differentiation with different PCB126 concentrations. Left panel: glucose uptake, right panel: fold increase in glucose uptake in response to insulin. C. Differentiated C2C12 myotubes were exposed for the last 24hrs of differentiation to the CM of 3T3-L1 adipocytes exposed to different PCB126 concentrations in IS or IR conditions. D. Differentiated mouse primary myotubes were exposed for the last 24hrs of differentiation to the CM of 3T3-L1 adipocytes exposed to different PCB126 concentrations in IS conditions. Left panel: glucose uptake, right panel: fold increase in glucose uptake in response to insulin. A-D. After differentiation and treatments, cells were subsequently treated ±100 nM insulin for 20 min and exposed to 10 μM 2-deoxy-glucose and 0.5 μCi/mL [ 3 H]2-deoxyglucose for 10 min. The absolute values of 2-deoxyglucose uptake under basal state (no insulin, no PCB126) were between 20 and 50 pmol/min/μg in 3T3-L1 adipocytes, between 50 and 80 pmol/min/μg in C2C12, and between 10 and 40 pmol/min/μg in mouse primary myotubes. Data are presented relative to the vehicle as mean ±SEM. n=3-4 independent experiments, each independent experiment was done in 3 replicates. *: P <0.05, **: P <0.01, ***: P <0.001 compared to 0 nM; #: P <0.05 compared with basal condition (no insulin).

    Journal: bioRxiv

    Article Title: PCB126-mediated effects on adipocyte energy metabolism and adipokine secretion may result in abnormal glucose uptake in muscle cells

    doi: 10.1101/2020.07.07.192245

    Figure Lengend Snippet: A-B. 3T3-L1 adipocytes were differentiated in IS (A) and IR (B) conditions and treated for the last 24hrs of differentiation with different PCB126 concentrations. Left panel: glucose uptake, right panel: fold increase in glucose uptake in response to insulin. C. Differentiated C2C12 myotubes were exposed for the last 24hrs of differentiation to the CM of 3T3-L1 adipocytes exposed to different PCB126 concentrations in IS or IR conditions. D. Differentiated mouse primary myotubes were exposed for the last 24hrs of differentiation to the CM of 3T3-L1 adipocytes exposed to different PCB126 concentrations in IS conditions. Left panel: glucose uptake, right panel: fold increase in glucose uptake in response to insulin. A-D. After differentiation and treatments, cells were subsequently treated ±100 nM insulin for 20 min and exposed to 10 μM 2-deoxy-glucose and 0.5 μCi/mL [ 3 H]2-deoxyglucose for 10 min. The absolute values of 2-deoxyglucose uptake under basal state (no insulin, no PCB126) were between 20 and 50 pmol/min/μg in 3T3-L1 adipocytes, between 50 and 80 pmol/min/μg in C2C12, and between 10 and 40 pmol/min/μg in mouse primary myotubes. Data are presented relative to the vehicle as mean ±SEM. n=3-4 independent experiments, each independent experiment was done in 3 replicates. *: P <0.05, **: P <0.01, ***: P <0.001 compared to 0 nM; #: P <0.05 compared with basal condition (no insulin).

    Article Snippet: C2C12 myoblasts (Sigma-Aldrich) were grown in low glucose DMEM, 10% fetal bovine serum (FBS, Wisent) and 1X AA.

    Techniques:

    A. 3T3-L1 adipocytes were differentiated in IS (left panel) and IR (right panel) conditions and treated for the last 24hrs of differentiation with different PCB126 concentrations. B. Differentiated C2C12 myotubes were exposed for the last 24hrs of differentiation to the CM of 3T3-L1 adipocytes exposed to different PCB126 concentrations in IS (left panel) or IR conditions (right panel). A-B. Glycolysis rates were estimated by measuring extracellular acidification rates (ECAR) with a Seahorse analyzer (Agilent). ECAR were first measured in resting conditions, and cells were then treated with 600 ng/mL oligomycin to determine maximal glycolytic capacity (M.G.C.). Data are presented relative to the vehicle as mean ±SEM. n=4 independent experiments, each independent experiment was done in 5 replicates. *: P <0.05, **, P <0.01, ***: P <0.001 compared to 0 nM.

    Journal: bioRxiv

    Article Title: PCB126-mediated effects on adipocyte energy metabolism and adipokine secretion may result in abnormal glucose uptake in muscle cells

    doi: 10.1101/2020.07.07.192245

    Figure Lengend Snippet: A. 3T3-L1 adipocytes were differentiated in IS (left panel) and IR (right panel) conditions and treated for the last 24hrs of differentiation with different PCB126 concentrations. B. Differentiated C2C12 myotubes were exposed for the last 24hrs of differentiation to the CM of 3T3-L1 adipocytes exposed to different PCB126 concentrations in IS (left panel) or IR conditions (right panel). A-B. Glycolysis rates were estimated by measuring extracellular acidification rates (ECAR) with a Seahorse analyzer (Agilent). ECAR were first measured in resting conditions, and cells were then treated with 600 ng/mL oligomycin to determine maximal glycolytic capacity (M.G.C.). Data are presented relative to the vehicle as mean ±SEM. n=4 independent experiments, each independent experiment was done in 5 replicates. *: P <0.05, **, P <0.01, ***: P <0.001 compared to 0 nM.

    Article Snippet: C2C12 myoblasts (Sigma-Aldrich) were grown in low glucose DMEM, 10% fetal bovine serum (FBS, Wisent) and 1X AA.

    Techniques:

    Levels of oxidative stress markers (catalase, glutathione peroxidase (GPx) 1 and 4, superoxide dismutase (SOD) 2, glutaredoxin (Grx) 2) in (A) 3T3-L1 adipocytes differentiated in IR conditions and exposed for 24hrs to different concentrations of PCB126 or (B) C2C12 myotubes exposed to the CM of PCB126-treated IR adipocytes. Top panel: quantification by density analysis, bottom panel: representative western blots. (A) α-tubulin and (B) GAPDH were used as loading controls. n=3-6 independent experiments. Data are presented relative to the vehicle as mean ±SEM. *: P <0.05 compared to 0 nM.

    Journal: bioRxiv

    Article Title: PCB126-mediated effects on adipocyte energy metabolism and adipokine secretion may result in abnormal glucose uptake in muscle cells

    doi: 10.1101/2020.07.07.192245

    Figure Lengend Snippet: Levels of oxidative stress markers (catalase, glutathione peroxidase (GPx) 1 and 4, superoxide dismutase (SOD) 2, glutaredoxin (Grx) 2) in (A) 3T3-L1 adipocytes differentiated in IR conditions and exposed for 24hrs to different concentrations of PCB126 or (B) C2C12 myotubes exposed to the CM of PCB126-treated IR adipocytes. Top panel: quantification by density analysis, bottom panel: representative western blots. (A) α-tubulin and (B) GAPDH were used as loading controls. n=3-6 independent experiments. Data are presented relative to the vehicle as mean ±SEM. *: P <0.05 compared to 0 nM.

    Article Snippet: C2C12 myoblasts (Sigma-Aldrich) were grown in low glucose DMEM, 10% fetal bovine serum (FBS, Wisent) and 1X AA.

    Techniques: Western Blot

    Levels of p-AMPK/AMPK in (A) 3T3-L1 adipocytes differentiated in IR conditions and exposed for 24hrs to 100 nM of PCB126 or (B) C2C12 exposed to the CM of IR adipocytes exposed to 100 nM PCB126. Left panel: quantification by density analysis, right panel: representative western blots. n=3 independent experiments. Data are presented relative to the vehicle as mean ±SEM. **: P ≤0.01 compared to 0 nM.

    Journal: bioRxiv

    Article Title: PCB126-mediated effects on adipocyte energy metabolism and adipokine secretion may result in abnormal glucose uptake in muscle cells

    doi: 10.1101/2020.07.07.192245

    Figure Lengend Snippet: Levels of p-AMPK/AMPK in (A) 3T3-L1 adipocytes differentiated in IR conditions and exposed for 24hrs to 100 nM of PCB126 or (B) C2C12 exposed to the CM of IR adipocytes exposed to 100 nM PCB126. Left panel: quantification by density analysis, right panel: representative western blots. n=3 independent experiments. Data are presented relative to the vehicle as mean ±SEM. **: P ≤0.01 compared to 0 nM.

    Article Snippet: C2C12 myoblasts (Sigma-Aldrich) were grown in low glucose DMEM, 10% fetal bovine serum (FBS, Wisent) and 1X AA.

    Techniques: Western Blot