Journal: Scientific Reports

Article Title: Cell fibers promote proliferation of co-cultured cells on a dish

doi: 10.1038/s41598-019-57213-0

Figure Lengend Snippet: Co-culture of a 3T3 fiber and C2C12 cells. ( a ) Low-magnification and ( b ) high-magnification images of a 3T3 fiber three days after formation. The core consisting of 3T3 fibroblasts and ECM was sealed with the alginate shell. The part close to the edges did not contain the cells. ( c ) Co-culture of a 3T3 fiber and C2C12 cells. The day-3 3T3 fiber was transferred to C2C12 culture in a dish to initiate the co-culture. ( d ) Control culture of an ECM-only fiber and C2C12 cells.

Article Snippet: Mouse C2C12 myoblasts were plated on tissue culture dishes coated with 1% gelatin (Sigma) at 1 × 10 4 cells per 35-mm diameter dish and cultured in DMEM (4500 mg/mL glucose) (Merck) containing 5% FBS (MP Biomedicals) and Penicillin-Streptomycin (Merck) (C2C12 medium) for 24 h before initiating co-culture with cell fibers or the control culture.

Techniques: Co-Culture Assay

Journal: Scientific Reports

Article Title: Cell fibers promote proliferation of co-cultured cells on a dish

doi: 10.1038/s41598-019-57213-0

Figure Lengend Snippet: The effect of 3T3 fibers on C2C12 cell proliferation. ( a ) Images of C2C12 cells after being cultured for two days in serum-free medium, with an ECM-only fiber, with a 3T3 fiber and in medium containing 5% FBS. Scale bars; 100 μm. (b ) The number of C2C12 cells per culture dish under each condition on day 0, day 1 and day 2. A 3T3 fiber promoted C2C12 cell proliferation significantly more (* P = 0.013; t -test) than an ECM-only fiber on day 2 did. Data are mean ± s.d. (n = 3). NS, not significant. ( c ) Images of immunofluorescence for the cell-proliferation marker, Ki-67 (red) under each condition on day 1. The cell nuclei were stained with Hoechst 33342 (blue). Scale bars; 100 μm. ( d ) The percentage of Ki67-positive C2C12 cells on day 1. The result showed a similar tendency in one of the cell number counting assays. Data are mean ± s.d. (n = 4).

Article Snippet: Mouse C2C12 myoblasts were plated on tissue culture dishes coated with 1% gelatin (Sigma) at 1 × 10 4 cells per 35-mm diameter dish and cultured in DMEM (4500 mg/mL glucose) (Merck) containing 5% FBS (MP Biomedicals) and Penicillin-Streptomycin (Merck) (C2C12 medium) for 24 h before initiating co-culture with cell fibers or the control culture.

Techniques: Cell Culture, Immunofluorescence, Marker, Staining

Journal: Scientific Reports

Article Title: Cell fibers promote proliferation of co-cultured cells on a dish

doi: 10.1038/s41598-019-57213-0

Figure Lengend Snippet: The effect of multiple 3T3 fibers on C2C12 cell proliferation. ( a ) Images of C2C12 cells after being cultured for two days with one or three 3T3 fibers. Scale bars; 100 μm. (b ) The number of C2C12 cells per dish under each condition on day 2. The increase in the number of C2C12 cells was proportional to the number of 3T3 fibers. Data are mean ± s.d. (n = 4).

Article Snippet: Mouse C2C12 myoblasts were plated on tissue culture dishes coated with 1% gelatin (Sigma) at 1 × 10 4 cells per 35-mm diameter dish and cultured in DMEM (4500 mg/mL glucose) (Merck) containing 5% FBS (MP Biomedicals) and Penicillin-Streptomycin (Merck) (C2C12 medium) for 24 h before initiating co-culture with cell fibers or the control culture.

Techniques: Cell Culture