Journal: Molecules

Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

doi: 10.3390/molecules25112583

Figure Lengend Snippet: Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus C18 column connected to a Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.

Article Snippet: Liquid Chromatography-Mass Spectrometry (LC-MS) Extracted samples from the local source were redissolved in 10 mL methanol and aliquots analyzed with a qTof LC-MS using Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA, USA), or analyzed after pre-purification carried out using a 1260 Infinity II HPLC system with a C18 column (250 × 4.6 mm, 5 μm particle size) (Waters, Milford, MA, USA).

Techniques:

Journal: Molecules

Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

doi: 10.3390/molecules25112583

Figure Lengend Snippet: Detection of 1,25(OH) 2 D3 in honey. ( A ) Whole extract was analyzed directly using LC-MS with a Zorbax Eclipse Plus C18 column with a methanol gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + . ( B ) The sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

Article Snippet: Liquid Chromatography-Mass Spectrometry (LC-MS) Extracted samples from the local source were redissolved in 10 mL methanol and aliquots analyzed with a qTof LC-MS using Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA, USA), or analyzed after pre-purification carried out using a 1260 Infinity II HPLC system with a C18 column (250 × 4.6 mm, 5 μm particle size) (Waters, Milford, MA, USA).

Techniques: Liquid Chromatography with Mass Spectroscopy, Purification

Journal: Molecules

Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

doi: 10.3390/molecules25112583

Figure Lengend Snippet: Detection of 25(OH)D3 in honey. After a pre-purification step using a C18 column with an acetonitrile gradient, the fraction was analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 383.331 [M + H − H 2 O] + .

Article Snippet: Liquid Chromatography-Mass Spectrometry (LC-MS) Extracted samples from the local source were redissolved in 10 mL methanol and aliquots analyzed with a qTof LC-MS using Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA, USA), or analyzed after pre-purification carried out using a 1260 Infinity II HPLC system with a C18 column (250 × 4.6 mm, 5 μm particle size) (Waters, Milford, MA, USA).

Techniques: Purification, Liquid Chromatography with Mass Spectroscopy

Journal: Molecules

Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

doi: 10.3390/molecules25112583

Figure Lengend Snippet: Detection of 1,20(OH) 2 D3 in honey. ( A ) The peak corresponding in RT to standard was collected from commercially available honey using an Atlantis C18 column using a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 439.319 [M + Na] + . ( B ) The local honey sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

Article Snippet: Liquid Chromatography-Mass Spectrometry (LC-MS) Extracted samples from the local source were redissolved in 10 mL methanol and aliquots analyzed with a qTof LC-MS using Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA, USA), or analyzed after pre-purification carried out using a 1260 Infinity II HPLC system with a C18 column (250 × 4.6 mm, 5 μm particle size) (Waters, Milford, MA, USA).

Techniques: Liquid Chromatography with Mass Spectroscopy, Purification

Journal: Molecules

Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

doi: 10.3390/molecules25112583

Figure Lengend Snippet: Detection of 20(OH)D3 and 20(OH)-7DHC in honey. ( A ) After a pre-purification step using an Atlantis C18 column with a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 423.324 [M + Na] + . ( B ) After extraction of commercially sourced honey, the sample was directly analyzed using LC-MS with a Pursuit 200Å PFP column with a methanol gradient. The EIC was obtained using m/z = 383.331 [M + H − H 2 O] + .

Article Snippet: Liquid Chromatography-Mass Spectrometry (LC-MS) Extracted samples from the local source were redissolved in 10 mL methanol and aliquots analyzed with a qTof LC-MS using Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA, USA), or analyzed after pre-purification carried out using a 1260 Infinity II HPLC system with a C18 column (250 × 4.6 mm, 5 μm particle size) (Waters, Milford, MA, USA).

Techniques: Purification, Liquid Chromatography with Mass Spectroscopy

Journal: Molecules

Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

doi: 10.3390/molecules25112583

Figure Lengend Snippet: Detection of D3, 7DHC and L3 in honey. Whole honey extract was analyzed directly using a Zorbax Eclipse Plus C18 column connected to a Xevo G2 XS equipped with an ACQUITY UPLC I-Class System (Waters, Milford, MA) using a methanol gradient. The extracted ion chromatogram (EIC) was obtained using m/z = 407.329 [M + Na] + . Arrow 1, RT of D3 standard; arrow 2, RT of 7DHC standard; arrow 3, RT of L3 standard.

Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

Techniques:

Journal: Molecules

Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

doi: 10.3390/molecules25112583

Figure Lengend Snippet: Detection of 1,20(OH) 2 D3 in honey. ( A ) The peak corresponding in RT to standard was collected from commercially available honey using an Atlantis C18 column using a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 439.319 [M + Na] + . ( B ) The local honey sample was pre-purified using a C18 column, then analyzed using LC-MS with a Zorbax Eclipse Plus C18 column with an acetonitrile gradient. The EIC was obtained using m/z = 399.326 [M + H − H 2 O] + .

Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

Techniques: Liquid Chromatography with Mass Spectroscopy, Purification

Journal: Molecules

Article Title: Detection of 7-Dehydrocholesterol and Vitamin D3 Derivatives in Honey

doi: 10.3390/molecules25112583

Figure Lengend Snippet: Detection of 20(OH)D3 and 20(OH)-7DHC in honey. ( A ) After a pre-purification step using an Atlantis C18 column with a methanol gradient, then analyzed using LC-MS with an ACQUITY UPLC BEH C18 column with a methanol gradient. The EIC was obtained using m/z = 423.324 [M + Na] + . ( B ) After extraction of commercially sourced honey, the sample was directly analyzed using LC-MS with a Pursuit 200Å PFP column with a methanol gradient. The EIC was obtained using m/z = 383.331 [M + H − H 2 O] + .

Article Snippet: Chromatography for LC-MS was performed using a Zorbax Eclipse Plus C18 column (2.1 × 50 mm, 1.8 μm) (Agilent Technology, Santa Clara, CA, USA), an ACQUITY UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm), an Atlantis C18 column (100 × 4.6 mm, 5 μm) (Waters, Milford, MA, USA) or a Pursuit 200Å PFP column (4.6 × 150 mm, 5 µm) (Agilent Technology, Santa Clara, CA, USA).

Techniques: Purification, Liquid Chromatography with Mass Spectroscopy

Journal: Scientific Reports

Article Title: Vitamin B2 as a virulence factor in Pseudogymnoascus destructans skin infection

doi: 10.1038/srep33200

Figure Lengend Snippet: Production of riboflavin in Pseudogymnoascus destructans . ( a ) HPLC chromatogram of SPE C18 extract from liquid cultivation medium of P. destructans strain 20631-21 T (see Fig. 6a,b for medium and blank controls). ( b ) Average production curve (±standard error) of riboflavin in six strains of P. destructans ( Fig. 3 ) shows continuous metabolite accumulation. ( c ) Average production curve of [Fe 3+ ] triacetylfusarinine C in six strains of P. destructans peaks after 6 to 8 weeks of culture. The concentration of the metabolite was corrected for biomass content in the fermentation medium.

Article Snippet: A Gemini 5 μm C18 column (250 × 4.6 mm, Phenomenex, Torrance, CA, USA) with a guard column was used for the analysis.

Techniques: High Performance Liquid Chromatography, Concentration Assay

Journal: Scientific Reports

Article Title: Vitamin B2 as a virulence factor in Pseudogymnoascus destructans skin infection

doi: 10.1038/srep33200

Figure Lengend Snippet: Production and tolerance of riboflavin in Pseudogymnoascus fungi. ( a ) HPLC chromatogram of SPE C18 extract from liquid cultivation medium of Pseudogymnoascus sp. strain CCF5026. ( b ) Blank liquid cultivation medium HPLC chromatogram. ( c ) Average production curves (±standard error) of riboflavin in non-pathogenic Pseudogymnoascus strains. The concentration of the metabolite was corrected for biomass content in the fermentation medium. ( d ) Tolerance of P. destructans strains to riboflavin in concentrations ranging from 0 to 200 μg ml −1 . Riboflavin was added after autoclaving the medium containing glucose (20 g l −1 ), yeast extract (5 g l −1 ), and agar (20 g l −1 ). Colony diameter was measured after 49 days. Three replicates were used in each experiment, and their values may overlap.

Article Snippet: A Gemini 5 μm C18 column (250 × 4.6 mm, Phenomenex, Torrance, CA, USA) with a guard column was used for the analysis.

Techniques: High Performance Liquid Chromatography, Concentration Assay

Journal: PLoS ONE

Article Title: A Drug Delivery Strategy: Binding Enkephalin to Asialoglycoprotein Receptor by Enzymatic Galactosylation

doi: 10.1371/journal.pone.0095024

Figure Lengend Snippet: Enzymatic transformation of Lac-Enk to Gal-Lac-Enk. A) RP-HPLC analysis of one-pot reaction for Lac-Enk with UDP-Glc and galactose epimerase. From top to bottom: substrate (Lac-Enk) only; positive control – reaction mixture containing UDP-Gal and LgtC; negative control – reaction mixture containing UDP-Glc and LgtC; reaction mixture containing UDP-Glc, LgtC and galactose epimerase. All reactions were conducted in 50 mM HEPES (pH 7), 10 mM MnCl 2 buffer at 37°C. RP-HPLC was conducted on an Alltima C18 column using a gradient of 0–90% solvent B, t R = 17.2 min (substrate, Lac-Enk) and t R = 16.8 min (product, Gal-Lac-Enk). B) ES-MS analysis of the acceptor, Lac-Enk m/z (C 44 H 63 N 7 O 18 , 977.4) m/z 978.5 [M+H] + (calcd. 978.4). C) ES-MS analysis of the product, Gal-Lac-Enk (C 52 H 76 N 8 O 23 , 1139.5) m/z 1140.5 [M+H] + (calcd. 1140.5).

Article Snippet: A Phenomenex Luna C18 column (5 µm, 50×2.0 mm, Torrance, USA) was used for LC-MS analysis.

Techniques: Transformation Assay, High Performance Liquid Chromatography, Gas Chromatography, Positive Control, Negative Control, Mass Spectrometry

Journal: PLoS ONE

Article Title: A Drug Delivery Strategy: Binding Enkephalin to Asialoglycoprotein Receptor by Enzymatic Galactosylation

doi: 10.1371/journal.pone.0095024

Figure Lengend Snippet: Stability profiles of enkephalin and carbohydrate-derived enkephalins. A) Plasma stability assay: the concentration of compounds in plasma was determined at various time points using RP-HPLC on a C18 Vydac column, gradient of 20–35% (enkephalin) or 0–70% solvent B (glycosylated enkephalins) over 30 min. B) Caco-2 cell stability assay: The concentration of compound in Caco-2 cell homogenates at various time points was quantified using LC-MS. Data presented is the mean ± S.D. (n = 3).

Article Snippet: A Phenomenex Luna C18 column (5 µm, 50×2.0 mm, Torrance, USA) was used for LC-MS analysis.

Techniques: Derivative Assay, Stability Assay, Concentration Assay, High Performance Liquid Chromatography, Liquid Chromatography with Mass Spectroscopy