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    ATCC c glutamicum atcc 13032
    Western blot analysis of protein extracts of C. <t>glutamicum</t> wild-type strain ATCC 13032, the cg0413 mutant (CGL2059), and the complemented cg0413 mutant [CGL2059(pCGL482- cg0413 )] with polyclonal anti-CgMytC antibodies.
    C Glutamicum Atcc 13032, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3348 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Western blot analysis of protein extracts of C. glutamicum wild-type strain ATCC 13032, the cg0413 mutant (CGL2059), and the complemented cg0413 mutant [CGL2059(pCGL482- cg0413 )] with polyclonal anti-CgMytC antibodies.

    Journal: Journal of Bacteriology

    Article Title: Identification of a Mycoloyl Transferase Selectively Involved in O-Acylation of Polypeptides in Corynebacteriales

    doi: 10.1128/JB.00285-13

    Figure Lengend Snippet: Western blot analysis of protein extracts of C. glutamicum wild-type strain ATCC 13032, the cg0413 mutant (CGL2059), and the complemented cg0413 mutant [CGL2059(pCGL482- cg0413 )] with polyclonal anti-CgMytC antibodies.

    Article Snippet: The corresponding DNA fragment (1,555 bp) was amplified by PCR from C. glutamicum ATCC 13032 chromosomal DNA using primer pair 336-Bam (5′-TTTGGGCGGATCCTAGCATT-3′)/336-Xho (5′-TTAACTCGAGTTCTAGGCCTCT-3′), digested by BamHI and XhoI, and ligated with pCGL482 to give pCGL482-CgMytC.

    Techniques: Western Blot, Mutagenesis

    Physical map of the tryptophan gene cluster in C. glutamicum ATCC 13032. Within the trpPEGDCFBA genes, indicated by blue arrows, the coloured bars denote the mapped integrations of 43 independent tryptophan auxotrophic mutants. The two orientations with respect to the genome are indicated in black (clockwise) and green (counterclockwise direction). The transposon integration identified by PCR screening is also marked (orange bar). The left stem-loop symbol denotes the transcriptional attenuator involved in the regulation of the tryptophan biosynthesis [43] and the right loop a Rho-independent transcriptional terminator structure.

    Journal: BMC Genomics

    Article Title: Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway

    doi: 10.1186/1471-2164-7-205

    Figure Lengend Snippet: Physical map of the tryptophan gene cluster in C. glutamicum ATCC 13032. Within the trpPEGDCFBA genes, indicated by blue arrows, the coloured bars denote the mapped integrations of 43 independent tryptophan auxotrophic mutants. The two orientations with respect to the genome are indicated in black (clockwise) and green (counterclockwise direction). The transposon integration identified by PCR screening is also marked (orange bar). The left stem-loop symbol denotes the transcriptional attenuator involved in the regulation of the tryptophan biosynthesis [43] and the right loop a Rho-independent transcriptional terminator structure.

    Article Snippet: Sequence data/accession numbers Nucleotide homology searches were applied against the annotated C. glutamicum ATCC 13032 genome sequence [GenBank: BX927147 ] [ , ] obtained from GenBank [ ].

    Techniques: Polymerase Chain Reaction

    Distribution of transposon insertions on a circular plot of the C. glutamicum ATCC 13032 genome [GenBank: BX927147 ]. Coloured bars of the outer circle pointing inward and outward show the orientation of the cointegrates with respect to the genome in clockwise and counterclockwise direction, respectively. Red bars indicate the integration positions mapped for randomly selected clones and black bars map those investigated by auxotrophy analysis. Additional circles (from inward to outward) represent relative G+C content and coding regions transcribed in clockwise and counterclockwise direction, respectively. A positive deviation in G+C content from the average (53.8%) is shown by bars pointing outward and a negative deviation by bars pointing inward. Annotated genes are coloured according to the colour scheme of the functional classes system COG (cluster of orthologous groups) [89]. The plot was generated with GenDB version 2.2 [90].

    Journal: BMC Genomics

    Article Title: Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway

    doi: 10.1186/1471-2164-7-205

    Figure Lengend Snippet: Distribution of transposon insertions on a circular plot of the C. glutamicum ATCC 13032 genome [GenBank: BX927147 ]. Coloured bars of the outer circle pointing inward and outward show the orientation of the cointegrates with respect to the genome in clockwise and counterclockwise direction, respectively. Red bars indicate the integration positions mapped for randomly selected clones and black bars map those investigated by auxotrophy analysis. Additional circles (from inward to outward) represent relative G+C content and coding regions transcribed in clockwise and counterclockwise direction, respectively. A positive deviation in G+C content from the average (53.8%) is shown by bars pointing outward and a negative deviation by bars pointing inward. Annotated genes are coloured according to the colour scheme of the functional classes system COG (cluster of orthologous groups) [89]. The plot was generated with GenDB version 2.2 [90].

    Article Snippet: Sequence data/accession numbers Nucleotide homology searches were applied against the annotated C. glutamicum ATCC 13032 genome sequence [GenBank: BX927147 ] [ , ] obtained from GenBank [ ].

    Techniques: Clone Assay, Functional Assay, Generated

    Dendrogram showing the relationship of inositol monophosphatase family proteins (IMP) in Actinobacteria and E. coli K-12. A multiple alignment with amino acid sequences of proteins with high similarity to the C. glutamicum IMPs was generated with the use of the DIALIGN2 software. Based on this alignment an unrooted phylogenetic tree was constructed using the neighbour-joining algorithm integrated in the CLUSTALX package and visualized as a radial tree by the TreeTool software. The branches were combined to classes that delivered within the bootstrapping analyses in at least two thirds of the cases the same subtree. These classes, marked by different colours, were named according to the designations in the boxed leaves. The locus tags (leaves) were obtained from the GenBank genome entries. C. glutamicum proteins are printed in bold letters. Locus tag prefixes denote following organisms (c, complete genome sequence; da, draft assembly): Arth ( Arthrobacter sp . FB24; da), BL ( Bifidobacterium longum NCC2705; c), BLinB01 ( Brevibacterium linens BL2; da), DIP ( Corynebacterium diphtheriae NCTC13129; c), CE ( C. efficiens YS-314; c), cg ( C. glutamicum ATCC 13032; c), jk ( C. jeikeium K411; c), Ecoli ( Escherichia coli K-12; c), Francci3 ( Frankia sp . CcI3; c), Franean1 ( Frankia sp . EAN1pec; da), JNB ( Janibacter sp . HTCC2649; da), Krad ( Kineococcus radiotolerans SRS30216; da), Lxx ( Leifsonia xyli subsp. xyli str. CTCB07; c), Micol ( Micromonospora olivasterospora ; da), MAP ( Mycobacterium avium subsp. paratuberculosis K-10; c), ML ( M. leprae TN; c), Rv ( M. tuberculosis H37Rv; c), Mycsm ( Mycobacterium smegmatis str . MC2 155; da), nfa ( Nocardia farcinica IFM 10152; c), Noca ( Nocardioides sp . JS614; da), PPA ( Propionibacterium acnes KPA171202; c), RhoDS7 ( Rhodococcus sp . DS7; da), Rxyl ( Rubrobacter xylanophilus DSM 9941; da), SAV ( Streptomyces avermitilis MA-4680; c), SCO ( S. coelicolor A3(2); c) and Tfu ( Thermobifida fusca YX; c).

    Journal: BMC Genomics

    Article Title: Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway

    doi: 10.1186/1471-2164-7-205

    Figure Lengend Snippet: Dendrogram showing the relationship of inositol monophosphatase family proteins (IMP) in Actinobacteria and E. coli K-12. A multiple alignment with amino acid sequences of proteins with high similarity to the C. glutamicum IMPs was generated with the use of the DIALIGN2 software. Based on this alignment an unrooted phylogenetic tree was constructed using the neighbour-joining algorithm integrated in the CLUSTALX package and visualized as a radial tree by the TreeTool software. The branches were combined to classes that delivered within the bootstrapping analyses in at least two thirds of the cases the same subtree. These classes, marked by different colours, were named according to the designations in the boxed leaves. The locus tags (leaves) were obtained from the GenBank genome entries. C. glutamicum proteins are printed in bold letters. Locus tag prefixes denote following organisms (c, complete genome sequence; da, draft assembly): Arth ( Arthrobacter sp . FB24; da), BL ( Bifidobacterium longum NCC2705; c), BLinB01 ( Brevibacterium linens BL2; da), DIP ( Corynebacterium diphtheriae NCTC13129; c), CE ( C. efficiens YS-314; c), cg ( C. glutamicum ATCC 13032; c), jk ( C. jeikeium K411; c), Ecoli ( Escherichia coli K-12; c), Francci3 ( Frankia sp . CcI3; c), Franean1 ( Frankia sp . EAN1pec; da), JNB ( Janibacter sp . HTCC2649; da), Krad ( Kineococcus radiotolerans SRS30216; da), Lxx ( Leifsonia xyli subsp. xyli str. CTCB07; c), Micol ( Micromonospora olivasterospora ; da), MAP ( Mycobacterium avium subsp. paratuberculosis K-10; c), ML ( M. leprae TN; c), Rv ( M. tuberculosis H37Rv; c), Mycsm ( Mycobacterium smegmatis str . MC2 155; da), nfa ( Nocardia farcinica IFM 10152; c), Noca ( Nocardioides sp . JS614; da), PPA ( Propionibacterium acnes KPA171202; c), RhoDS7 ( Rhodococcus sp . DS7; da), Rxyl ( Rubrobacter xylanophilus DSM 9941; da), SAV ( Streptomyces avermitilis MA-4680; c), SCO ( S. coelicolor A3(2); c) and Tfu ( Thermobifida fusca YX; c).

    Article Snippet: Sequence data/accession numbers Nucleotide homology searches were applied against the annotated C. glutamicum ATCC 13032 genome sequence [GenBank: BX927147 ] [ , ] obtained from GenBank [ ].

    Techniques: Generated, Software, Construct, Sequencing

    Experimental design for quantification of flux parameters at the pyruvate node of Corynebacterium glutamicum with an equimolar mixture of [ 13 C 6 ] glucose and naturally labelled glucose . Relative change of the mass isotopomer distribution of aspartate (m/z 418) with varied Φ PEPC (A), relative change of the mass isotopomer distribution of valine (m/z 288) with varied ζ PEPC/PEPCK (B), relative change of the mass isotopomer distribution of alanine (m/z 260) with varied ζ PC/MAE (C). The labelling patterns at sole contribution of PEPC (Φ PEPC = 100 %), and highly reversible fluxes at the pyruvate node (ζ PEPC/PEPCK = ζ PC/MAE = 10) are taken as reference point and set to 100 %. The flux parameters investigated here comprise Φ PEPC (flux partitioning between PEPC and PC), ζ PEPC/PEPCK (ratio of exchange flux to net flux between the pools of PEP and OAA/MAL catalyzed by PEPC and PEPCK) and ζ PC/MAE (ratio of exchange flux to net flux between the pools of PYR and OAA/MAL catalyzed by PC and MAE). The exact definitions for the flux parameters are given in the appendix. Unless varied the flux parameters reflect the situation for the parent strain C. glutamicum lysC fbr (Figure 4).

    Journal: Microbial Cell Factories

    Article Title: Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum

    doi: 10.1186/1475-2859-7-8

    Figure Lengend Snippet: Experimental design for quantification of flux parameters at the pyruvate node of Corynebacterium glutamicum with an equimolar mixture of [ 13 C 6 ] glucose and naturally labelled glucose . Relative change of the mass isotopomer distribution of aspartate (m/z 418) with varied Φ PEPC (A), relative change of the mass isotopomer distribution of valine (m/z 288) with varied ζ PEPC/PEPCK (B), relative change of the mass isotopomer distribution of alanine (m/z 260) with varied ζ PC/MAE (C). The labelling patterns at sole contribution of PEPC (Φ PEPC = 100 %), and highly reversible fluxes at the pyruvate node (ζ PEPC/PEPCK = ζ PC/MAE = 10) are taken as reference point and set to 100 %. The flux parameters investigated here comprise Φ PEPC (flux partitioning between PEPC and PC), ζ PEPC/PEPCK (ratio of exchange flux to net flux between the pools of PEP and OAA/MAL catalyzed by PEPC and PEPCK) and ζ PC/MAE (ratio of exchange flux to net flux between the pools of PYR and OAA/MAL catalyzed by PC and MAE). The exact definitions for the flux parameters are given in the appendix. Unless varied the flux parameters reflect the situation for the parent strain C. glutamicum lysC fbr (Figure 4).

    Article Snippet: Using a site specific primer set of forward and reverse primer (Table ), the obtained DNA fragment was shortened by about 1500 bp when genomic DNA of lysCfbr Δpyk was used as template instead of genomic DNA of the reference strain C. glutamicum ATCC 13032.

    Techniques:

    The ilvBNCD gene locus in C. glutamicum ATCC 13032. Within the locus of about 9 kb is located the ilvBNC ), as well as ilvD . These are separated by orfx , an ORF of unknown function.

    Journal: Applied and Environmental Microbiology

    Article Title: Linking Central Metabolism with Increased Pathway Flux: l-Valine Accumulation by Corynebacterium glutamicum

    doi: 10.1128/AEM.68.5.2246-2250.2002

    Figure Lengend Snippet: The ilvBNCD gene locus in C. glutamicum ATCC 13032. Within the locus of about 9 kb is located the ilvBNC ), as well as ilvD . These are separated by orfx , an ORF of unknown function.

    Article Snippet: The gene bank of the genomic DNA of wild-type C. glutamicum ATCC 13032 is also described by Vrljić et al. ( ).

    Techniques:

    Analyses of [ 14 C]glucose uptake (A) and ptsG transcription (B) in WT C. glutamicum , C. glutamicum Δ pgi , C. glutamicum Δ pgi (pEKEx3), C. glutamicum Δ pgi (pEKEx3- pgi ), C. glutamicum Δ pgi (pBB1), and C. glutamicum Δ pgi

    Journal: Applied and Environmental Microbiology

    Article Title: Phosphotransferase System-Mediated Glucose Uptake Is Repressed in Phosphoglucoisomerase-Deficient Corynebacterium glutamicum Strains

    doi: 10.1128/AEM.03231-12

    Figure Lengend Snippet: Analyses of [ 14 C]glucose uptake (A) and ptsG transcription (B) in WT C. glutamicum , C. glutamicum Δ pgi , C. glutamicum Δ pgi (pEKEx3), C. glutamicum Δ pgi (pEKEx3- pgi ), C. glutamicum Δ pgi (pBB1), and C. glutamicum Δ pgi

    Article Snippet: Genes were amplified via PCR from genomic DNA of wild-type (WT) C. glutamicum ATCC 13032 or E. coli MG1655 using the oligonucleotide primers listed in .

    Techniques:

    Growth (circles) and glucose consumption (triangles) of WT C. glutamicum (black symbols) and C. glutamicum Δ pgi (light gray symbols) (A), of C. glutamicum Δ pgi (pEKEx3) (dark gray symbols) and C. glutamicum Δ pgi (pEKEx3- pgi ) (open

    Journal: Applied and Environmental Microbiology

    Article Title: Phosphotransferase System-Mediated Glucose Uptake Is Repressed in Phosphoglucoisomerase-Deficient Corynebacterium glutamicum Strains

    doi: 10.1128/AEM.03231-12

    Figure Lengend Snippet: Growth (circles) and glucose consumption (triangles) of WT C. glutamicum (black symbols) and C. glutamicum Δ pgi (light gray symbols) (A), of C. glutamicum Δ pgi (pEKEx3) (dark gray symbols) and C. glutamicum Δ pgi (pEKEx3- pgi ) (open

    Article Snippet: Genes were amplified via PCR from genomic DNA of wild-type (WT) C. glutamicum ATCC 13032 or E. coli MG1655 using the oligonucleotide primers listed in .

    Techniques: