Journal: BMC Genomics
Article Title: Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway
Figure Lengend Snippet: Dendrogram showing the relationship of inositol monophosphatase family proteins (IMP) in Actinobacteria and E. coli K-12. A multiple alignment with amino acid sequences of proteins with high similarity to the C. glutamicum IMPs was generated with the use of the DIALIGN2 software. Based on this alignment an unrooted phylogenetic tree was constructed using the neighbour-joining algorithm integrated in the CLUSTALX package and visualized as a radial tree by the TreeTool software. The branches were combined to classes that delivered within the bootstrapping analyses in at least two thirds of the cases the same subtree. These classes, marked by different colours, were named according to the designations in the boxed leaves. The locus tags (leaves) were obtained from the GenBank genome entries. C. glutamicum proteins are printed in bold letters. Locus tag prefixes denote following organisms (c, complete genome sequence; da, draft assembly): Arth ( Arthrobacter sp . FB24; da), BL ( Bifidobacterium longum NCC2705; c), BLinB01 ( Brevibacterium linens BL2; da), DIP ( Corynebacterium diphtheriae NCTC13129; c), CE ( C. efficiens YS-314; c), cg ( C. glutamicum ATCC 13032; c), jk ( C. jeikeium K411; c), Ecoli ( Escherichia coli K-12; c), Francci3 ( Frankia sp . CcI3; c), Franean1 ( Frankia sp . EAN1pec; da), JNB ( Janibacter sp . HTCC2649; da), Krad ( Kineococcus radiotolerans SRS30216; da), Lxx ( Leifsonia xyli subsp. xyli str. CTCB07; c), Micol ( Micromonospora olivasterospora ; da), MAP ( Mycobacterium avium subsp. paratuberculosis K-10; c), ML ( M. leprae TN; c), Rv ( M. tuberculosis H37Rv; c), Mycsm ( Mycobacterium smegmatis str . MC2 155; da), nfa ( Nocardia farcinica IFM 10152; c), Noca ( Nocardioides sp . JS614; da), PPA ( Propionibacterium acnes KPA171202; c), RhoDS7 ( Rhodococcus sp . DS7; da), Rxyl ( Rubrobacter xylanophilus DSM 9941; da), SAV ( Streptomyces avermitilis MA-4680; c), SCO ( S. coelicolor A3(2); c) and Tfu ( Thermobifida fusca YX; c).
Article Snippet: Sequence data/accession numbers Nucleotide homology searches were applied against the annotated C. glutamicum ATCC 13032 genome sequence [GenBank: BX927147 ] [ , ] obtained from GenBank [ ].
Techniques: Generated, Software, Construct, Sequencing