Journal: Frontiers in Molecular Neuroscience
Article Title: SIRT1/PGC-1α Signaling Promotes Mitochondrial Functional Recovery and Reduces Apoptosis after Intracerebral Hemorrhage in Rats
Figure Lengend Snippet: Expression levels of apoptotic proteins after ICH injury following SIRT1 activation in rats. Representative western blots of AIF, cytochrome c, caspase-3 (c3), and cleaved caspase-3 (cleaved c3) (A,B) . Relative protein band density values were calculated as the ratio of the protein of interest to that of GAPDH. Quantification of (A,B) is shown in (C,D) , respectively. Error bars represent mean ± SEM. (C) ( ∗ P
Article Snippet: Antibodies used were as follows: SIRT1 (1:500, Cell Signaling Technologies, Danvers, MA), PGC-1α (1:1,000, Abcam, Cambridge, MA, United States), AMP-activated protein kinase (AMPK) (1:1,000, Proteintech), phosphorylated AMP-activated protein kinase (pAMPK) (1:500, Bioworld), acetylated-lysine (1:500, Bioworld), lamin B1 (Cell Signaling Technologies), GAPDH (1:2000, Bioworld), ATP synthase β (ATPβ) (1:400, Bioworld), cytochrome c oxidase subunit I (1:400, Bioworld), NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 8 (NDUFB8) (1:400, Bioworld), cytochrome c (1:1,000, Abcam), AIF (1:1,000, Santa Cruz, Dallas, TX), cleaved caspase-3 (cleaved c3) (1:1,000, Cell Signaling Technologies, Danvers, MA), and caspase-3 (c3) (1:500, Santa Cruz).
Techniques: Expressing, Activation Assay, Western Blot