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  • 99
    Thermo Fisher vascular endothelial growth factor vegf a
    The secretion of growth factors involved in the wound-healing process was increased in UCX® spheroids. Hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1, granulocyte-colony stimulating factor (G-CSF), vascular endothelial growth factor <t>(VEGF)-A,</t> fibroblast growth factor (FGF)-2 and interleukin (IL)-6 were quantified in two-dimensional conditioned medium (CM2D) and three-dimensional conditioned medium (CM3D) using a FlowCytomix™ kit. Keratinocyte growth factor (KGF) was quantified in CM2D and CM3D by enzyme-linked immunosorbent assay. Results are expressed as fold-increase of CM3D relative to CM2D. Data are shown in mean ± standard deviation; n = 3.
    Vascular Endothelial Growth Factor Vegf A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial growth factor vegf a/product/Thermo Fisher
    Average 99 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    vascular endothelial growth factor vegf a - by Bioz Stars, 2020-09
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    90
    Bio-Rad bio plex protm human cytokine 17 plex immunoassay
    The secretion of growth factors involved in the wound-healing process was increased in UCX® spheroids. Hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1, granulocyte-colony stimulating factor (G-CSF), vascular endothelial growth factor <t>(VEGF)-A,</t> fibroblast growth factor (FGF)-2 and interleukin (IL)-6 were quantified in two-dimensional conditioned medium (CM2D) and three-dimensional conditioned medium (CM3D) using a FlowCytomix™ kit. Keratinocyte growth factor (KGF) was quantified in CM2D and CM3D by enzyme-linked immunosorbent assay. Results are expressed as fold-increase of CM3D relative to CM2D. Data are shown in mean ± standard deviation; n = 3.
    Bio Plex Protm Human Cytokine 17 Plex Immunoassay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio plex protm human cytokine 17 plex immunoassay/product/Bio-Rad
    Average 90 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    bio plex protm human cytokine 17 plex immunoassay - by Bioz Stars, 2020-09
    90/100 stars
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    91
    Thermo Fisher human il 22 flowcytomix simplex kit
    The secretion of growth factors involved in the wound-healing process was increased in UCX® spheroids. Hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1, granulocyte-colony stimulating factor (G-CSF), vascular endothelial growth factor <t>(VEGF)-A,</t> fibroblast growth factor (FGF)-2 and interleukin (IL)-6 were quantified in two-dimensional conditioned medium (CM2D) and three-dimensional conditioned medium (CM3D) using a FlowCytomix™ kit. Keratinocyte growth factor (KGF) was quantified in CM2D and CM3D by enzyme-linked immunosorbent assay. Results are expressed as fold-increase of CM3D relative to CM2D. Data are shown in mean ± standard deviation; n = 3.
    Human Il 22 Flowcytomix Simplex Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human il 22 flowcytomix simplex kit/product/Thermo Fisher
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    human il 22 flowcytomix simplex kit - by Bioz Stars, 2020-09
    91/100 stars
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    93
    Thermo Fisher keratinocyte growth factor
    The secretion of growth factors involved in the wound-healing process was increased in UCX® spheroids. Hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1, granulocyte-colony stimulating factor (G-CSF), vascular endothelial growth factor (VEGF)-A, fibroblast growth factor (FGF)-2 and interleukin (IL)-6 were quantified in two-dimensional conditioned medium (CM2D) and three-dimensional conditioned medium (CM3D) using a FlowCytomix™ kit. <t>Keratinocyte</t> growth factor (KGF) was quantified in CM2D and CM3D by enzyme-linked immunosorbent assay. Results are expressed as fold-increase of CM3D relative to CM2D. Data are shown in mean ± standard deviation; n = 3.
    Keratinocyte Growth Factor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 75 article reviews
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    keratinocyte growth factor - by Bioz Stars, 2020-09
    93/100 stars
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    89
    Thermo Fisher fluorescent bead immunoassay kit
    The secretion of growth factors involved in the wound-healing process was increased in UCX® spheroids. Hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1, granulocyte-colony stimulating factor (G-CSF), vascular endothelial growth factor (VEGF)-A, fibroblast growth factor (FGF)-2 and interleukin (IL)-6 were quantified in two-dimensional conditioned medium (CM2D) and three-dimensional conditioned medium (CM3D) using a FlowCytomix™ kit. <t>Keratinocyte</t> growth factor (KGF) was quantified in CM2D and CM3D by enzyme-linked immunosorbent assay. Results are expressed as fold-increase of CM3D relative to CM2D. Data are shown in mean ± standard deviation; n = 3.
    Fluorescent Bead Immunoassay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent bead immunoassay kit/product/Thermo Fisher
    Average 89 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    fluorescent bead immunoassay kit - by Bioz Stars, 2020-09
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    Image Search Results


    The secretion of growth factors involved in the wound-healing process was increased in UCX® spheroids. Hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1, granulocyte-colony stimulating factor (G-CSF), vascular endothelial growth factor (VEGF)-A, fibroblast growth factor (FGF)-2 and interleukin (IL)-6 were quantified in two-dimensional conditioned medium (CM2D) and three-dimensional conditioned medium (CM3D) using a FlowCytomix™ kit. Keratinocyte growth factor (KGF) was quantified in CM2D and CM3D by enzyme-linked immunosorbent assay. Results are expressed as fold-increase of CM3D relative to CM2D. Data are shown in mean ± standard deviation; n = 3.

    Journal: Stem Cell Research & Therapy

    Article Title: Three-dimensional spheroid cell culture of umbilical cord tissue-derived mesenchymal stromal cells leads to enhanced paracrine induction of wound healing

    doi: 10.1186/s13287-015-0082-5

    Figure Lengend Snippet: The secretion of growth factors involved in the wound-healing process was increased in UCX® spheroids. Hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1, granulocyte-colony stimulating factor (G-CSF), vascular endothelial growth factor (VEGF)-A, fibroblast growth factor (FGF)-2 and interleukin (IL)-6 were quantified in two-dimensional conditioned medium (CM2D) and three-dimensional conditioned medium (CM3D) using a FlowCytomix™ kit. Keratinocyte growth factor (KGF) was quantified in CM2D and CM3D by enzyme-linked immunosorbent assay. Results are expressed as fold-increase of CM3D relative to CM2D. Data are shown in mean ± standard deviation; n = 3.

    Article Snippet: Growth factor quantification The concentrations of hepatocyte growth factor (HGF), fibroblast growth factor (FGF)-2, vascular endothelial growth factor (VEGF)-A, interleukin (IL)-6, TGF-β1, keratinocyte growth factor (KGF) and C-GSF in the CM2D, CM3D and control sample were evaluated by means of a Fluorescent Bead Immunoassay kit (FlowCytomix™, eBioscience) and an enzyme-linked immunosorbent assay (ELISA) kit (Quantikine®, R & D Systems, Minneapolis, MI, USA), for KGF quantification.

    Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

    The secretion of growth factors involved in the wound-healing process was increased in UCX® spheroids. Hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1, granulocyte-colony stimulating factor (G-CSF), vascular endothelial growth factor (VEGF)-A, fibroblast growth factor (FGF)-2 and interleukin (IL)-6 were quantified in two-dimensional conditioned medium (CM2D) and three-dimensional conditioned medium (CM3D) using a FlowCytomix™ kit. Keratinocyte growth factor (KGF) was quantified in CM2D and CM3D by enzyme-linked immunosorbent assay. Results are expressed as fold-increase of CM3D relative to CM2D. Data are shown in mean ± standard deviation; n = 3.

    Journal: Stem Cell Research & Therapy

    Article Title: Three-dimensional spheroid cell culture of umbilical cord tissue-derived mesenchymal stromal cells leads to enhanced paracrine induction of wound healing

    doi: 10.1186/s13287-015-0082-5

    Figure Lengend Snippet: The secretion of growth factors involved in the wound-healing process was increased in UCX® spheroids. Hepatocyte growth factor (HGF), transforming growth factor (TGF)-β1, granulocyte-colony stimulating factor (G-CSF), vascular endothelial growth factor (VEGF)-A, fibroblast growth factor (FGF)-2 and interleukin (IL)-6 were quantified in two-dimensional conditioned medium (CM2D) and three-dimensional conditioned medium (CM3D) using a FlowCytomix™ kit. Keratinocyte growth factor (KGF) was quantified in CM2D and CM3D by enzyme-linked immunosorbent assay. Results are expressed as fold-increase of CM3D relative to CM2D. Data are shown in mean ± standard deviation; n = 3.

    Article Snippet: Growth factor quantification The concentrations of hepatocyte growth factor (HGF), fibroblast growth factor (FGF)-2, vascular endothelial growth factor (VEGF)-A, interleukin (IL)-6, TGF-β1, keratinocyte growth factor (KGF) and C-GSF in the CM2D, CM3D and control sample were evaluated by means of a Fluorescent Bead Immunoassay kit (FlowCytomix™, eBioscience) and an enzyme-linked immunosorbent assay (ELISA) kit (Quantikine®, R & D Systems, Minneapolis, MI, USA), for KGF quantification.

    Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

    UCX® spheroid-derived conditioned medium (CM3D) enhances immortalized keratinocyte cell line (HaCaT) and human dermal fibroblasts (HDF) cell migration, proliferation and elastin production. (A) Graphs represent the average area occupied by the HaCaT cells after 40 hours in contact with serum-free conditioned medium from three-dimensional cultures (CM3D), two-dimensional cultures (CM2D) and control (basal medium). Data are shown as mean ± standard deviation (SD); n = 9 to 12. (B) Representative images of HaCaT scratch-wound assays immediately after the scratches had been made (0 h) and then after 40 hours in the presence of serum-free CM3D, CM2D and control. (C) Graphs represent the average area occupied by the HDF after 40 hours with CM3D, CM2D and control. Data are shown as mean ± SD; n = 6 to 9. (D) Representative images of HDF scratch-wound assays immediately after the scratches had been made (0 h) and then after 40 hours in the presence of serum-free CM3D, CM2D and control. (E) HaCaT and HDF viability evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) reduction assay after 48 hours of incubation with CM2D and CM3D. Data are shown as percentage of control (Crtl) and as mean ± standard error of the mean; n = 3. (F) Elastin production by HaCaT and HDF at 24 and 72 hours post-incubation with CM3D, CM2D and control. Data are shown as mean ± SD; n = 2. * P

    Journal: Stem Cell Research & Therapy

    Article Title: Three-dimensional spheroid cell culture of umbilical cord tissue-derived mesenchymal stromal cells leads to enhanced paracrine induction of wound healing

    doi: 10.1186/s13287-015-0082-5

    Figure Lengend Snippet: UCX® spheroid-derived conditioned medium (CM3D) enhances immortalized keratinocyte cell line (HaCaT) and human dermal fibroblasts (HDF) cell migration, proliferation and elastin production. (A) Graphs represent the average area occupied by the HaCaT cells after 40 hours in contact with serum-free conditioned medium from three-dimensional cultures (CM3D), two-dimensional cultures (CM2D) and control (basal medium). Data are shown as mean ± standard deviation (SD); n = 9 to 12. (B) Representative images of HaCaT scratch-wound assays immediately after the scratches had been made (0 h) and then after 40 hours in the presence of serum-free CM3D, CM2D and control. (C) Graphs represent the average area occupied by the HDF after 40 hours with CM3D, CM2D and control. Data are shown as mean ± SD; n = 6 to 9. (D) Representative images of HDF scratch-wound assays immediately after the scratches had been made (0 h) and then after 40 hours in the presence of serum-free CM3D, CM2D and control. (E) HaCaT and HDF viability evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) reduction assay after 48 hours of incubation with CM2D and CM3D. Data are shown as percentage of control (Crtl) and as mean ± standard error of the mean; n = 3. (F) Elastin production by HaCaT and HDF at 24 and 72 hours post-incubation with CM3D, CM2D and control. Data are shown as mean ± SD; n = 2. * P

    Article Snippet: Growth factor quantification The concentrations of hepatocyte growth factor (HGF), fibroblast growth factor (FGF)-2, vascular endothelial growth factor (VEGF)-A, interleukin (IL)-6, TGF-β1, keratinocyte growth factor (KGF) and C-GSF in the CM2D, CM3D and control sample were evaluated by means of a Fluorescent Bead Immunoassay kit (FlowCytomix™, eBioscience) and an enzyme-linked immunosorbent assay (ELISA) kit (Quantikine®, R & D Systems, Minneapolis, MI, USA), for KGF quantification.

    Techniques: Derivative Assay, Migration, Standard Deviation, MTT Assay, Incubation