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All (6)
Abnova
Sino Biological
MilliporeSigma
Upstate Biotech
Carna Biosciences
Creative BioMart
 

About 11 products / 32 snippets / 1 images

93 / 100
Bioz Stars
Mouse SRC Kinase / Proto-oncogene c-Src Protein
Sino Biological  
Catalog#: 50311-M20B
Techniques
 
Article Snippets
"and CCl 4 were purchased from Sigma-Aldrich. mSRC (50311-M20B)and EP were purchased from Sino Biological "  (see more)
94 / 100
Bioz Stars
SRC
Carna Biosciences  
Catalog#: KINASES
Techniques
 
Article Snippets
"and donkey anti-rabbit Alexa Fluor Plus 488 (#A32790, RRID: AB_2762833; polyclonal IgG, Invitrogen). The following recombinant proteins were used: CaMKIIα (#PR4586C, Invitrogen), GST-CaMKIIβ (#02-110, Carna biosciences), recombinant neuronal c-Src ( 18 ) and Calpain-1 (#208713-500UG, Calbiochem). Chemicals and reagents specifically used for proteomic analysis include Pierce high pH reversed-phase "  (see more)
93 / 100
Bioz Stars
Mouse SRC Kinase / Proto-oncogene c-Src Protein
Sino Biological  
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Catalog#: 50311-M20B
Techniques
 
Article Snippets
"and CCl 4 were purchased from Sigma-Aldrich. mSRC (50311-M20B)and EP were purchased from Sino Biological "  (see more)
92 / 100
Bioz Stars
Mouse CSK / C-Src kinase Protein
Sino Biological  
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Catalog#: 50893-M20B
Techniques
 
Article Snippets
"P (#9804, CST) and 1 μg of recombinant mouse CSK (#50893-M20B, Sino Biological, Beijing, China) or 1 μg o"  (see more)
( A and B ) IP/IB analysis of HEK293T cells based on exogenously expressed proteins. The SBM in <t>CSK</t> and its mutation in CSK-AAA are indicated. ( C ) FA competition binding assay. Peptides containing the candidate SBM of CSK and the mutated sequence CSK-AAA compete with fluorescein-Puc 91–106 for binding to the SPOP MATH domain. [L] is the peptide concentration. Continuous lines are nonlinear least-squares fits to a complete competitive binding model. See for K d values. ( D ) Clustal Omega–based sequence alignment of Src family kinases and CSK. The SBM and the conserved tyrosine are highlighted by green and red boxes, respectively. ( E ) IP/IB analysis of HEK293T cells based on exogenously expressed proteins. ( F ) Nuclear translocation assay of SPOP-deficient RAW264.7 cells that were reconstituted with indicated forms of SPOP. ( G ) qPCR analysis of cells described in (F) that were stimulated with CpG-DNA for 4 hours. ( H to K ) In vitro kinase assays based on proteins expressed in HEK293T cells. <t>CSK-AAA,</t> <t>SST340/341/341AAA.</t> Fold differences of CSK autophosphorylation as determined by phosphor image analysis. The activity used for reference (set as 1) is underlined. Data represent mean ± SD from three independent experiments. ** P < 0.01, *** P < 0.005 are determined by two-way ANOVA with Sidak’s multiple comparison test (G).
MUTA
BIND-A
SEQ
CO-A
NUC-TLA
c-Abl tyrosine kinase activity is elevated in cells that express oncogenic <t>Src.</t> (A) Murine BaF3 cells containing either vector alone (−) or zinc-inducible v-src (+) were treated with 75 μm zinc chloride. c-Abl tyrosine kinase activity was determined by immunoprecipitation with K12 antibody (top), and c-Abl protein level (8E9) (middle), or Src protein level (EC10) (bottom) were analyzed for each time point. (B) c-Abl and Src kinase activities from murine C3H10T1/2 fibroblasts expressing vector alone, wild-type chicken c-Src, or activated chicken v-Src were examined in triton-soluble and -insoluble lysates by immunoprecipitation with PEX4 (Abl) or GD11 (Src) antibodies. In vitro kinase assays were performed with GST–Crk (Abl) or Sam68 (Src) as substrates. Abl and <t>c-Src/v-Src</t> levels were determined as above. The assay represents one of three independent experiments with K12 or PEX4 antibody with mean increased c-Abl activity of 22.5- ± 9.6-fold (K12) as compared with vector control in triton-soluble lysates.
ACT-A
PLASMID-P
IP
EXP
INVITRO
PELP1 regulates STAT3 Ser727 phosphorylation via the <t>src-MAPK</t> pathway. A, COS-1 cells were cotransfected with or without PELP1 and treated with EGF for 15 minutes. The total lysates were immunoblotted with a phospho-MAPK–specific antibody. B, MCF-7 cells stably expressing pcDNA or PELP1 were treated with EGF for various periods of time, and MAPK activation was analyzed by Western blot analysis with a phosphospecific antibody. C, MCF-7 cells stably expressing pcDNA or PELP1 were pretreated with or without the MAPK inhibitor PD98059 for 60 minutes and then treated with EGF for 15 minutes. MAPK activation was analyzed by Western blotting. D, MCF-7 cells were cotransfected with the STAT3 luciferase reporter gene with or without PELP1. The cells were pretreated with or without PD98059 and stimulated with EGF for 8 hours. The STAT3 reporter gene activity was determined. E, MCF-7 cells stably expressing pcDNA or PELP1 were pretreated with or without the src inhibitor PP2 for 60 minutes and then treated with EGF for 15 minutes. The status of MAPK activation and STAT3 Ser727 phosphorylation was analyzed by immunoblotting. F, murine fibroblast cells deficient in src kinase <t>(SYF</t> cells) were transfected with or without PELP1 and treated with or without EGF, and STAT Ser727 phosphorylation was measured by immunoblotting.
STAB-TRANS
EXP
ACTIV
WB
LUCIF
Activation of c-Abl by PDGF involves <t>Src</t> family kinases. (A) Serum-starved 10T1/2 cells stably expressing wild-type chicken c-Src were stimulated with PDGF-BB and/or EGF. Kinase assay represents one of three independent experiments with mean maximal activation 3.3 ± 0.4 for EGF/PDGF stimulation. (B) Spontaneously immortalized MEFs from Fyn−/−, Src+/−, and Yes−/− mice were infected with retrovirus containing either vector, wild-type mammalian c-Src, or kinase-inactive Src (K297M). c-Abl kinase assays were performed on stimulated and unstimulated cells after serum starvation with Ab3 anti-Abl antibody for immunoprecipitations. Src protein levels were determined by Western blot analysis with Src2 antibody. c-Abl levels were determined as above. c-Abl activation was decreased 40%–60% in cells expressing kinase-inactive Src as compared with vector in three independent experiments. (C) NIH-3T3 cells were infected with retroviruses containing either vector, wild-type, or a kinase-inactive form of Src (K297M). Kinase assays were performed as above with c-Abl K12 antibody. c-Abl activation was decreased 40%–75% in cells expressing kinase-inactive Src as compared with vector in three independent experiments. (D) Ph cells expressing wild-type (Wt), kinase-inactive (K−), or Src-binding mutant (F72/74) α/β chimeric PDGF receptors were serum starved, stimulated with PDGF-AA, and c-Abl kinase assays were performed as above with Pex4 antibody. c-Abl and Src/Fyn/Yes proteins levels were assessed as above. Protein levels of α/β chimeric receptors were determined by Western blot analysis with 80.8 antibody.
ACTIV
STAB-TRANS
EXP
KA
INFECT
×
93 / 100
Bioz Stars
Human SRC Kinase / Proto-oncogene c-Src Protein
Sino Biological  
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Catalog#: 10755-H20B
Techniques
 
Article Snippets
"human SRC kinase (final 1.14 µM in each reaction, #10755-H20B, Sino Biological) diluted in PBS was mixed "  (see more)
94 / 100
Bioz Stars
SRC
Carna Biosciences  
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Catalog#: KINASES
Techniques
 
Article Snippets
"and donkey anti-rabbit Alexa Fluor Plus 488 (#A32790, RRID: AB_2762833; polyclonal IgG, Invitrogen). The following recombinant proteins were used: CaMKIIα (#PR4586C, Invitrogen), GST-CaMKIIβ (#02-110, Carna biosciences), recombinant neuronal c-Src ( 18 ) and Calpain-1 (#208713-500UG, Calbiochem). Chemicals and reagents specifically used for proteomic analysis include Pierce high pH reversed-phase "  (see more)
91 / 100
Bioz Stars
C-Src kinase activity
MilliporeSigma / Sigma Aldrich  
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Techniques
 
Article Snippets
".. was assayed for c-Src kinase activity by incubating with .... prepared acid-denatured enolase (Sigma) (5 μg of .."  (see more)
85 / 100
Bioz Stars
Kinase active c-Src cDNA (Y527F)
Upstate Biotech  
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Techniques
 
Article Snippets
"Briefly, dominant negative c-Src cDNA (K295R, Y527F) and kinase active c-Src cDNA (Y527F) were obtained from Upstate Biotechnology as a cloning vector and subcloned into the cloning vector, .."  (see more)
80 / 100
Bioz Stars
Active c-Src kinase protein
MilliporeSigma  
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Techniques
 
Article Snippets
"Active c-Src kinase protein (N-terminal 6His-tagged recombinant human c-Src, molecular weight 61.7 kDa) was purchased from Milipore (Billerica, MA)."  (see more)
80 / 100
Bioz Stars
C-Src Kinase Activity Assay Kit
Abnova  
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Techniques
 
Article Snippets
"PKCδ and c-Src activity were gauged by PKC Kinase Activity Assay Kit (Assay Designs, Inc., Ann Arbor, MI, USA) and c-Src Kinase Activity Assay Kit (Abnova, Corp., Taipei, Taiwan)."  (see more)
85 / 100
Bioz Stars
Active human c-Src kinase
Upstate Biotech  
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Techniques
 
Article Snippets
"Active human c-Src kinase (recombinant enzyme expressed in Sf9 insect cells; obtained from Upstate Biotechnology Inc. product 14-117) was diluted immediately prior to .."  (see more)
80 / 100
Bioz Stars
Constitutive active human cSrc kinase (p60c-Src)
Upstate Biotech  
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Techniques
 
Article Snippets
"Abstract: In the COS-7 cell signalling network high levels of cAMP produced, for example, by co-stimulation of β2-adrenergic receptor (β2-AR) and bradykinin B2 receptor (BKR) may affect epidermal growth factor receptor (EGFR)-mediated activat ..."  (see more)
90 / 100
Bioz vStars
recombinant human c src tyrosine kinase gst tagged active
Creative BioMart  
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Catalog#: CSK-288H
Description
"Recombinant full length human CSK was expressed by baculovirus inSf9 insect cellsusing a N terminal GST tag MW 78 kDa CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase"  (see more)