bx51 light microscope Search Results


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  • 93
    Olympus bx51 light microscope
    Aged mice develop emphysema and airway remodeling after 3 months of CS exposure A. Lung function measurements for dynamic lung compliance were performed in intubated animals after 2 months of CS exposure and in tracheostomized animals after 3 months of CS exposure. B. Representative micrographs of HE-stained lung tissue sections from young and old FA and CS-exposed mice; scale bar 200 μm. C. Quantitative measurement of emphysema was determined by design-based stereology of HE-stained lung tissue sections using an Olympus <t>BX51</t> light microscope equipped with the computer-assisted stereological toolbox newCAST. D. Representative micrographs of Masson's Trichrome staining of lung tissue sections from young and old FA and CS-exposed mice; scale bar 100 μm. E. Total volume of airway collagen per basal membrane was determined via quantitative morphological assessment. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with ** p
    Bx51 Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 93/100, based on 2940 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 light microscope/product/Olympus
    Average 93 stars, based on 2940 article reviews
    Price from $9.99 to $1999.99
    bx51 light microscope - by Bioz Stars, 2020-09
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    88
    Olympus bx51 upright light microscope
    Aged mice develop emphysema and airway remodeling after 3 months of CS exposure A. Lung function measurements for dynamic lung compliance were performed in intubated animals after 2 months of CS exposure and in tracheostomized animals after 3 months of CS exposure. B. Representative micrographs of HE-stained lung tissue sections from young and old FA and CS-exposed mice; scale bar 200 μm. C. Quantitative measurement of emphysema was determined by design-based stereology of HE-stained lung tissue sections using an Olympus <t>BX51</t> light microscope equipped with the computer-assisted stereological toolbox newCAST. D. Representative micrographs of Masson's Trichrome staining of lung tissue sections from young and old FA and CS-exposed mice; scale bar 100 μm. E. Total volume of airway collagen per basal membrane was determined via quantitative morphological assessment. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with ** p
    Bx51 Upright Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 88/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 upright light microscope/product/Olympus
    Average 88 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    bx51 upright light microscope - by Bioz Stars, 2020-09
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    93
    Olympus bx51 compound light microscope
    Reactive oxygen species (ROS) accumulation upon wounding and flg22 induction is altered in omg1 leaves compared to Col-0 leaves. (A and B) DCF-DA ROS assay performed on wounded leaf tissues reveals that OMG1 affects cellular ROS production. (A) DCF-DA is a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell. A pipette tip was used to wound the surface of the leaves from the WT line, omg1 KO line and rbohd KO line (a known ROS-deficient mutant). Upon wounding, ROS is usually produced around the site of injury. The DCF-DA reagent is then oxidized by ROS resulting in appearance of green fluorescence. Plants were wounded and immediately placed in the DCF-DA solution for 20 min before visualized on the light microscope. (i) WT Col-0 leaves without wounding, (ii) WT Col-0 leaves, (iii) omg1 leaves and (iv) rbohd leaves were placed in DCF-DA solution immediately after wounding. In the WT plants green fluorescence around the wound site indicates the production of ROS. However, in the omg1 KO line and the rbohd KO line, ROS production was not detected. Images were taken on the Olympus <t>BX51</t> compound scope at 10× magnification, exposure time 55 ms. Scale bars: 100 µm. (B) Quantification of fluorescence was performed using integrated density (IntDen) and corrected total cell fluorescence (CTCF) [CTCF = Integrated density − (Area of selected cell × Mean fluorescence of background readings)] in ImageJ. Statistical analyses were performed using Student’s t -test, bar graphs with different letters show significant difference ( P
    Bx51 Compound Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 compound light microscope/product/Olympus
    Average 93 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    bx51 compound light microscope - by Bioz Stars, 2020-09
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    90
    Olympus photomicroscope olympus bx51 light microscope
    Reactive oxygen species (ROS) accumulation upon wounding and flg22 induction is altered in omg1 leaves compared to Col-0 leaves. (A and B) DCF-DA ROS assay performed on wounded leaf tissues reveals that OMG1 affects cellular ROS production. (A) DCF-DA is a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell. A pipette tip was used to wound the surface of the leaves from the WT line, omg1 KO line and rbohd KO line (a known ROS-deficient mutant). Upon wounding, ROS is usually produced around the site of injury. The DCF-DA reagent is then oxidized by ROS resulting in appearance of green fluorescence. Plants were wounded and immediately placed in the DCF-DA solution for 20 min before visualized on the light microscope. (i) WT Col-0 leaves without wounding, (ii) WT Col-0 leaves, (iii) omg1 leaves and (iv) rbohd leaves were placed in DCF-DA solution immediately after wounding. In the WT plants green fluorescence around the wound site indicates the production of ROS. However, in the omg1 KO line and the rbohd KO line, ROS production was not detected. Images were taken on the Olympus <t>BX51</t> compound scope at 10× magnification, exposure time 55 ms. Scale bars: 100 µm. (B) Quantification of fluorescence was performed using integrated density (IntDen) and corrected total cell fluorescence (CTCF) [CTCF = Integrated density − (Area of selected cell × Mean fluorescence of background readings)] in ImageJ. Statistical analyses were performed using Student’s t -test, bar graphs with different letters show significant difference ( P
    Photomicroscope Olympus Bx51 Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/photomicroscope olympus bx51 light microscope/product/Olympus
    Average 90 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    photomicroscope olympus bx51 light microscope - by Bioz Stars, 2020-09
    90/100 stars
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    90
    Olympus bx51 epifluorescent light microscope
    Reactive oxygen species (ROS) accumulation upon wounding and flg22 induction is altered in omg1 leaves compared to Col-0 leaves. (A and B) DCF-DA ROS assay performed on wounded leaf tissues reveals that OMG1 affects cellular ROS production. (A) DCF-DA is a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell. A pipette tip was used to wound the surface of the leaves from the WT line, omg1 KO line and rbohd KO line (a known ROS-deficient mutant). Upon wounding, ROS is usually produced around the site of injury. The DCF-DA reagent is then oxidized by ROS resulting in appearance of green fluorescence. Plants were wounded and immediately placed in the DCF-DA solution for 20 min before visualized on the light microscope. (i) WT Col-0 leaves without wounding, (ii) WT Col-0 leaves, (iii) omg1 leaves and (iv) rbohd leaves were placed in DCF-DA solution immediately after wounding. In the WT plants green fluorescence around the wound site indicates the production of ROS. However, in the omg1 KO line and the rbohd KO line, ROS production was not detected. Images were taken on the Olympus <t>BX51</t> compound scope at 10× magnification, exposure time 55 ms. Scale bars: 100 µm. (B) Quantification of fluorescence was performed using integrated density (IntDen) and corrected total cell fluorescence (CTCF) [CTCF = Integrated density − (Area of selected cell × Mean fluorescence of background readings)] in ImageJ. Statistical analyses were performed using Student’s t -test, bar graphs with different letters show significant difference ( P
    Bx51 Epifluorescent Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 epifluorescent light microscope/product/Olympus
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    bx51 epifluorescent light microscope - by Bioz Stars, 2020-09
    90/100 stars
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    88
    Olympus bx51 fluorescence light microscope
    Reactive oxygen species (ROS) accumulation upon wounding and flg22 induction is altered in omg1 leaves compared to Col-0 leaves. (A and B) DCF-DA ROS assay performed on wounded leaf tissues reveals that OMG1 affects cellular ROS production. (A) DCF-DA is a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell. A pipette tip was used to wound the surface of the leaves from the WT line, omg1 KO line and rbohd KO line (a known ROS-deficient mutant). Upon wounding, ROS is usually produced around the site of injury. The DCF-DA reagent is then oxidized by ROS resulting in appearance of green fluorescence. Plants were wounded and immediately placed in the DCF-DA solution for 20 min before visualized on the light microscope. (i) WT Col-0 leaves without wounding, (ii) WT Col-0 leaves, (iii) omg1 leaves and (iv) rbohd leaves were placed in DCF-DA solution immediately after wounding. In the WT plants green fluorescence around the wound site indicates the production of ROS. However, in the omg1 KO line and the rbohd KO line, ROS production was not detected. Images were taken on the Olympus <t>BX51</t> compound scope at 10× magnification, exposure time 55 ms. Scale bars: 100 µm. (B) Quantification of fluorescence was performed using integrated density (IntDen) and corrected total cell fluorescence (CTCF) [CTCF = Integrated density − (Area of selected cell × Mean fluorescence of background readings)] in ImageJ. Statistical analyses were performed using Student’s t -test, bar graphs with different letters show significant difference ( P
    Bx51 Fluorescence Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 fluorescence light microscope/product/Olympus
    Average 88 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    bx51 fluorescence light microscope - by Bioz Stars, 2020-09
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    92
    Olympus bx51 trf fluorescent light microscope
    Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus <t>BX51</t> fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.
    Bx51 Trf Fluorescent Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 trf fluorescent light microscope/product/Olympus
    Average 92 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    bx51 trf fluorescent light microscope - by Bioz Stars, 2020-09
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    85
    Olympus bx51 34fl 1 k o light microscope
    Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus <t>BX51</t> fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.
    Bx51 34fl 1 K O Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 34fl 1 k o light microscope/product/Olympus
    Average 85 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
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    92
    Olympus bx51 binocular light microscope
    Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus <t>BX51</t> fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.
    Bx51 Binocular Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 binocular light microscope/product/Olympus
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bx51 binocular light microscope - by Bioz Stars, 2020-09
    92/100 stars
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    92
    Olympus bx51 epifluorescence light microscope
    Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus <t>BX51</t> fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.
    Bx51 Epifluorescence Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 epifluorescence light microscope/product/Olympus
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bx51 epifluorescence light microscope - by Bioz Stars, 2020-09
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    88
    Olympus bx51 model udo3 light microscope
    Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus <t>BX51</t> fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.
    Bx51 Model Udo3 Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 88/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 model udo3 light microscope/product/Olympus
    Average 88 stars, based on 49 article reviews
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    91
    Olympus bx51 phase contrast light microscope
    Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus <t>BX51</t> fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.
    Bx51 Phase Contrast Light Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 phase contrast light microscope/product/Olympus
    Average 91 stars, based on 10 article reviews
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    bx51 phase contrast light microscope - by Bioz Stars, 2020-09
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    92
    Olympus bx51 tf upright transmitted light fluorescence microscope
    Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus <t>BX51</t> fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.
    Bx51 Tf Upright Transmitted Light Fluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bx51 tf upright transmitted light fluorescence microscope/product/Olympus
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    bx51 tf upright transmitted light fluorescence microscope - by Bioz Stars, 2020-09
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    85
    Olympus light microscope model bx51
    Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus <t>BX51</t> fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.
    Light Microscope Model Bx51, supplied by Olympus, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/light microscope model bx51/product/Olympus
    Average 85 stars, based on 4 article reviews
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    light microscope model bx51 - by Bioz Stars, 2020-09
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    Image Search Results


    Aged mice develop emphysema and airway remodeling after 3 months of CS exposure A. Lung function measurements for dynamic lung compliance were performed in intubated animals after 2 months of CS exposure and in tracheostomized animals after 3 months of CS exposure. B. Representative micrographs of HE-stained lung tissue sections from young and old FA and CS-exposed mice; scale bar 200 μm. C. Quantitative measurement of emphysema was determined by design-based stereology of HE-stained lung tissue sections using an Olympus BX51 light microscope equipped with the computer-assisted stereological toolbox newCAST. D. Representative micrographs of Masson's Trichrome staining of lung tissue sections from young and old FA and CS-exposed mice; scale bar 100 μm. E. Total volume of airway collagen per basal membrane was determined via quantitative morphological assessment. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with ** p

    Journal: Oncotarget

    Article Title: Inflammaging increases susceptibility to cigarette smoke-induced COPD

    doi: 10.18632/oncotarget.4027

    Figure Lengend Snippet: Aged mice develop emphysema and airway remodeling after 3 months of CS exposure A. Lung function measurements for dynamic lung compliance were performed in intubated animals after 2 months of CS exposure and in tracheostomized animals after 3 months of CS exposure. B. Representative micrographs of HE-stained lung tissue sections from young and old FA and CS-exposed mice; scale bar 200 μm. C. Quantitative measurement of emphysema was determined by design-based stereology of HE-stained lung tissue sections using an Olympus BX51 light microscope equipped with the computer-assisted stereological toolbox newCAST. D. Representative micrographs of Masson's Trichrome staining of lung tissue sections from young and old FA and CS-exposed mice; scale bar 100 μm. E. Total volume of airway collagen per basal membrane was determined via quantitative morphological assessment. Data were combined from 2 independent experiments with n = 8 and are given as mean values ± SD; one-way ANOVA following Bonferroni post test with ** p

    Article Snippet: Quantitative morphometry Design-based stereology was used to analyze sections using an Olympus BX51 light microscope equipped with a computer-assisted stereological toolbox (newCAST, Visiopharm, Hoersholm, Denmark) on HE- or Masson's Trichrome stained lung tissue slides as previously described [ ].

    Techniques: Mouse Assay, Staining, Light Microscopy

    Live/dead assay images of the inner surface of the scaffolds, captured with Olympus BX 51 fluorescence microscope, broadband blue excitation with interference band-eliminator filter U-MWIB3: excitation filter Olympus BP460-495, beam splitter Olympus DM505, band-eliminator filter BA510F; living cells, green; dead cells, red. The scale bar is 400 μm.

    Journal: Materials

    Article Title: 3D Powder Printed Bioglass and β-Tricalcium Phosphate Bone Scaffolds

    doi: 10.3390/ma11010013

    Figure Lengend Snippet: Live/dead assay images of the inner surface of the scaffolds, captured with Olympus BX 51 fluorescence microscope, broadband blue excitation with interference band-eliminator filter U-MWIB3: excitation filter Olympus BP460-495, beam splitter Olympus DM505, band-eliminator filter BA510F; living cells, green; dead cells, red. The scale bar is 400 μm.

    Article Snippet: The images were taken with Olympus BX 51 Light microscope.

    Techniques: Live Dead Assay, Fluorescence, Microscopy

    PCNA-positive germ cells of mice from GEN treatment. “NC” means negativehentrol. “CON, LGE and HGE” represent the control group, 40mg/kg GEN of the dietary group, and 800mg/kg of the dietary group, respectively. The magnification is 20×, OLYMPUS BX51 . Positive germ cells are marked by red arrows.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Oral Exposure to Genistein during Conception and Lactation Period Affects the Testicular Development of Male Offspring Mice

    doi: 10.3390/ani10030377

    Figure Lengend Snippet: PCNA-positive germ cells of mice from GEN treatment. “NC” means negativehentrol. “CON, LGE and HGE” represent the control group, 40mg/kg GEN of the dietary group, and 800mg/kg of the dietary group, respectively. The magnification is 20×, OLYMPUS BX51 . Positive germ cells are marked by red arrows.

    Article Snippet: The histological structure was then observed under a light microscope BX51 (OLYMPUS, Tokyo, Japan).

    Techniques: Mouse Assay

    Cellular localization of opsins in projection areas at different time points post-injection. ( a ) Retrogradely transduced neurons in layer III, as well as axons and dendrites in all cortical layers show high expression of opsin constructs in monkey O. A direct comparison of area LIP in animals O (8.5 weeks post-injection) and H (2 years post-injection) shows opsin expression in all parts of retrogradely transduced neurons, as well as potentials axonal projections from FEF in monkey O ( a , b ), but mainly in the cell body and dendrites in monkey H ( c ). Opsins were present in axonal projections to layer I of LIP in monkey O ( d ) but could be barely detected in layer I of LIP in monkey H ( e ). Retrogradely transduced neurons in layer III of area MT showed a similar distribution of opsins within cell bodies and dendrites in monkey O ( f ) and monkey H ( g ). Images were captured with a BX51 Olympus light microscope, controlled by Cell^B software, version 3.4 (Olympus).

    Journal: Scientific Reports

    Article Title: Histological assessment of optogenetic tools to study fronto-visual and fronto-parietal cortical networks in the rhesus macaque

    doi: 10.1038/s41598-020-67752-6

    Figure Lengend Snippet: Cellular localization of opsins in projection areas at different time points post-injection. ( a ) Retrogradely transduced neurons in layer III, as well as axons and dendrites in all cortical layers show high expression of opsin constructs in monkey O. A direct comparison of area LIP in animals O (8.5 weeks post-injection) and H (2 years post-injection) shows opsin expression in all parts of retrogradely transduced neurons, as well as potentials axonal projections from FEF in monkey O ( a , b ), but mainly in the cell body and dendrites in monkey H ( c ). Opsins were present in axonal projections to layer I of LIP in monkey O ( d ) but could be barely detected in layer I of LIP in monkey H ( e ). Retrogradely transduced neurons in layer III of area MT showed a similar distribution of opsins within cell bodies and dendrites in monkey O ( f ) and monkey H ( g ). Images were captured with a BX51 Olympus light microscope, controlled by Cell^B software, version 3.4 (Olympus).

    Article Snippet: Additional histological documentation (Fig. ) was made by a BX51 Olympus light microscope with a Color View I Camera, controlled by Cell^B software, version 3.4 (Olympus).

    Techniques: Injection, Expressing, Construct, Light Microscopy, Software

    Reactive oxygen species (ROS) accumulation upon wounding and flg22 induction is altered in omg1 leaves compared to Col-0 leaves. (A and B) DCF-DA ROS assay performed on wounded leaf tissues reveals that OMG1 affects cellular ROS production. (A) DCF-DA is a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell. A pipette tip was used to wound the surface of the leaves from the WT line, omg1 KO line and rbohd KO line (a known ROS-deficient mutant). Upon wounding, ROS is usually produced around the site of injury. The DCF-DA reagent is then oxidized by ROS resulting in appearance of green fluorescence. Plants were wounded and immediately placed in the DCF-DA solution for 20 min before visualized on the light microscope. (i) WT Col-0 leaves without wounding, (ii) WT Col-0 leaves, (iii) omg1 leaves and (iv) rbohd leaves were placed in DCF-DA solution immediately after wounding. In the WT plants green fluorescence around the wound site indicates the production of ROS. However, in the omg1 KO line and the rbohd KO line, ROS production was not detected. Images were taken on the Olympus BX51 compound scope at 10× magnification, exposure time 55 ms. Scale bars: 100 µm. (B) Quantification of fluorescence was performed using integrated density (IntDen) and corrected total cell fluorescence (CTCF) [CTCF = Integrated density − (Area of selected cell × Mean fluorescence of background readings)] in ImageJ. Statistical analyses were performed using Student’s t -test, bar graphs with different letters show significant difference ( P

    Journal: AoB Plants

    Article Title: A member of the CONSTANS-Like protein family is a putative regulator of reactive oxygen species homeostasis and spaceflight physiological adaptation

    doi: 10.1093/aobpla/ply075

    Figure Lengend Snippet: Reactive oxygen species (ROS) accumulation upon wounding and flg22 induction is altered in omg1 leaves compared to Col-0 leaves. (A and B) DCF-DA ROS assay performed on wounded leaf tissues reveals that OMG1 affects cellular ROS production. (A) DCF-DA is a fluorogenic dye that measures hydroxyl, peroxyl and other ROS activity within the cell. A pipette tip was used to wound the surface of the leaves from the WT line, omg1 KO line and rbohd KO line (a known ROS-deficient mutant). Upon wounding, ROS is usually produced around the site of injury. The DCF-DA reagent is then oxidized by ROS resulting in appearance of green fluorescence. Plants were wounded and immediately placed in the DCF-DA solution for 20 min before visualized on the light microscope. (i) WT Col-0 leaves without wounding, (ii) WT Col-0 leaves, (iii) omg1 leaves and (iv) rbohd leaves were placed in DCF-DA solution immediately after wounding. In the WT plants green fluorescence around the wound site indicates the production of ROS. However, in the omg1 KO line and the rbohd KO line, ROS production was not detected. Images were taken on the Olympus BX51 compound scope at 10× magnification, exposure time 55 ms. Scale bars: 100 µm. (B) Quantification of fluorescence was performed using integrated density (IntDen) and corrected total cell fluorescence (CTCF) [CTCF = Integrated density − (Area of selected cell × Mean fluorescence of background readings)] in ImageJ. Statistical analyses were performed using Student’s t -test, bar graphs with different letters show significant difference ( P

    Article Snippet: Leaves were then rinsed in standard buffer and observed under GFP bandpass filters using the Olympus BX51 compound light microscope at 10× magnification with 55 ms exposure time.

    Techniques: ROS Assay, Activity Assay, Transferring, Mutagenesis, Produced, Fluorescence, Light Microscopy, Mass Spectrometry

    Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus BX51 fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.

    Journal: Applied and Environmental Microbiology

    Article Title: In Vivo Self-Assembly of Stable Green Fluorescent Protein Fusion Particles and Their Uses in Enzyme Immobilization

    doi: 10.1128/AEM.00323-14

    Figure Lengend Snippet: Fluorescence microscopy analysis of E. coli BL21(DE3) cells containing GFP particles. Images were obtained at ×1,000 magnification using a U-MWIBA2 Blue excitation filter cube fitted to an Olympus BX51 fluorescent light microscope (Olympus Optical Co., Japan), an Optronics camera (Optronics, USA), and MagnaFire, version 2.1C, application software (Optronics, USA). Panels are as follows: A, GiCLZ; B, GNLZ; C, GiCLN; D, GNLN; E, GiCLB; F, GiCLO. G, GFP; iC, inactive PhaC; L, linker; N, NanA; B, BLA; O, OpdA; Z, ZZ.

    Article Snippet: Images of recombinant E. coli BL21(DE3) cells containing GFP particles and of GFP particles postextraction were obtained using an Olympus BX51 fluorescent light microscope (Olympus Optical Co., Ltd., Japan).

    Techniques: Fluorescence, Microscopy, Light Microscopy, Software