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    New England Biolabs bst dna polymerase large fragment
    Bst Dna Polymerase Large Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs large fragment bst 2 0 dna polymerase
    Large Fragment Bst 2 0 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    New England Biolabs bst dna polymerase large fragment bst
    Effect of dNTPs, ATP, PPi and APS on light output in BART. Simulation of effects of different ingredients on the light output in LAMP-BART in a “deficient mix” lacking primers and <t>Bst</t> polymerase but containing all other components as described in each case below. (A) Light output detected using varying concentrations of an equimolar mixture of four dNTPs. Light output peaks at 500 µM total dNTP concentration. (B) Light output detected using varying concentrations of ATP in the presence of 250 µM equimolar dNTPs. Light output is higher than in panel (A) and reaches saturation at 100 µM ATP, showing greater sensitivity to ATP. (C) Inhibitory effect of different concentrations of PPi on the light emission in the presence of 250 µM dNTPs and 100 µM ATP. (D) Stimulatory effect of increasing concentrations of APS on the light emission in the presence of 250 µM dNTPs and 100 µM PPi. (E) Effect of different concentrations of APS on BART curves in complete LAMP-BART formulation with 10 7 ChAT target <t>DNA</t> (red – 100 µM, navy – 200 µM, brown – 500 µM, green – 750 µM, blue - 1000 µM). As APS concentration is increased, there is little effect on peaking time but more PP i is converted to ATP resulting in a lower rate of inhibition of luciferase and a slower “switch off” of light output.
    Bst Dna Polymerase Large Fragment Bst, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs bst 2 0 warmstart dna polymerase
    Effect of dNTPs, ATP, PPi and APS on light output in BART. Simulation of effects of different ingredients on the light output in LAMP-BART in a “deficient mix” lacking primers and <t>Bst</t> polymerase but containing all other components as described in each case below. (A) Light output detected using varying concentrations of an equimolar mixture of four dNTPs. Light output peaks at 500 µM total dNTP concentration. (B) Light output detected using varying concentrations of ATP in the presence of 250 µM equimolar dNTPs. Light output is higher than in panel (A) and reaches saturation at 100 µM ATP, showing greater sensitivity to ATP. (C) Inhibitory effect of different concentrations of PPi on the light emission in the presence of 250 µM dNTPs and 100 µM ATP. (D) Stimulatory effect of increasing concentrations of APS on the light emission in the presence of 250 µM dNTPs and 100 µM PPi. (E) Effect of different concentrations of APS on BART curves in complete LAMP-BART formulation with 10 7 ChAT target <t>DNA</t> (red – 100 µM, navy – 200 µM, brown – 500 µM, green – 750 µM, blue - 1000 µM). As APS concentration is increased, there is little effect on peaking time but more PP i is converted to ATP resulting in a lower rate of inhibition of luciferase and a slower “switch off” of light output.
    Bst 2 0 Warmstart Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of dNTPs, ATP, PPi and APS on light output in BART. Simulation of effects of different ingredients on the light output in LAMP-BART in a “deficient mix” lacking primers and Bst polymerase but containing all other components as described in each case below. (A) Light output detected using varying concentrations of an equimolar mixture of four dNTPs. Light output peaks at 500 µM total dNTP concentration. (B) Light output detected using varying concentrations of ATP in the presence of 250 µM equimolar dNTPs. Light output is higher than in panel (A) and reaches saturation at 100 µM ATP, showing greater sensitivity to ATP. (C) Inhibitory effect of different concentrations of PPi on the light emission in the presence of 250 µM dNTPs and 100 µM ATP. (D) Stimulatory effect of increasing concentrations of APS on the light emission in the presence of 250 µM dNTPs and 100 µM PPi. (E) Effect of different concentrations of APS on BART curves in complete LAMP-BART formulation with 10 7 ChAT target DNA (red – 100 µM, navy – 200 µM, brown – 500 µM, green – 750 µM, blue - 1000 µM). As APS concentration is increased, there is little effect on peaking time but more PP i is converted to ATP resulting in a lower rate of inhibition of luciferase and a slower “switch off” of light output.

    Journal: PLoS ONE

    Article Title: Novel Bioluminescent Quantitative Detection of Nucleic Acid Amplification in Real-Time

    doi: 10.1371/journal.pone.0014155

    Figure Lengend Snippet: Effect of dNTPs, ATP, PPi and APS on light output in BART. Simulation of effects of different ingredients on the light output in LAMP-BART in a “deficient mix” lacking primers and Bst polymerase but containing all other components as described in each case below. (A) Light output detected using varying concentrations of an equimolar mixture of four dNTPs. Light output peaks at 500 µM total dNTP concentration. (B) Light output detected using varying concentrations of ATP in the presence of 250 µM equimolar dNTPs. Light output is higher than in panel (A) and reaches saturation at 100 µM ATP, showing greater sensitivity to ATP. (C) Inhibitory effect of different concentrations of PPi on the light emission in the presence of 250 µM dNTPs and 100 µM ATP. (D) Stimulatory effect of increasing concentrations of APS on the light emission in the presence of 250 µM dNTPs and 100 µM PPi. (E) Effect of different concentrations of APS on BART curves in complete LAMP-BART formulation with 10 7 ChAT target DNA (red – 100 µM, navy – 200 µM, brown – 500 µM, green – 750 µM, blue - 1000 µM). As APS concentration is increased, there is little effect on peaking time but more PP i is converted to ATP resulting in a lower rate of inhibition of luciferase and a slower “switch off” of light output.

    Article Snippet: Materials and reagents Unless otherwise noted, chemicals were purchased from Sigma with the exception of luciferin potassium salt (LH2 ; Europa Biotech, Ely, UK), UltraGlow firefly luciferase (UGrLuc; Promega, WI, USA), adenosine-5′-O-phosphosulphate (APS; Biolog Life Science Institute, Bremen, Germany), Bst DNA polymerase large fragment (Bst) and ThermoPol buffer (New England Biolabs, MA, USA), QuantiTech SYBR Green PCR kit (Qiagen, Hilden, Germany), cloned AMV reverse transcriptase and PicoGreen dsDNA Quantitation kit (Invitrogen, CA, USA).

    Techniques: Concentration Assay, Inhibition, Luciferase