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  • 99
    New England Biolabs bst dna polymerase
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Bst Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1716 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst dna polymerase/product/New England Biolabs
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    99
    Millipore bst dna polymerase
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Bst Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst dna polymerase/product/Millipore
    Average 99 stars, based on 9 article reviews
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    93
    Eiken Chemical bst dna polymerase
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Bst Dna Polymerase, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 93/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst dna polymerase/product/Eiken Chemical
    Average 93 stars, based on 129 article reviews
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    92
    Promega bst dna polymerase
    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the <t>Bst</t> <t>DNA</t> polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6
    Bst Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst dna polymerase/product/Promega
    Average 92 stars, based on 4 article reviews
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    90
    New England Biolabs bst dna polymerase buffer
    The SMART assay. ( a ) Specific probes hybridise with the target to form a three-way junction (3WJ), assisted by facilitator probes (f1 f2). The 3WJ initially contains a single-stranded, inactive T7 RNA polymerase promoter sequence. The promoter is made double stranded (active) by extension (by <t>Bst</t> <t>DNA</t> polymerase) off the 3' of the extension probe, leading to the generation of large amounts of RNA signal (by T7 RNA polymerase), which may itself be amplified if required. ( b ) Detection of RNA signal by ELOSA (Enzyme Linked OligoSorbant Assay). The assay uses 2 specific probes: a biotinylated capture probe and enzyme (Alkaline phosphatase, AP) linked detection probe. Non-specific nucleic acid and 3WJ probes are removed, following binding in a streptavidin coated well, and RNA signal is detected via a colour change. Quantification of signal takes place in a 96 well plate, allowing multiple samples to be analysed simultaneously.
    Bst Dna Polymerase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst dna polymerase buffer/product/New England Biolabs
    Average 90 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
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    92
    Bio-Rad bst dna polymerase
    The SMART assay. ( a ) Specific probes hybridise with the target to form a three-way junction (3WJ), assisted by facilitator probes (f1 f2). The 3WJ initially contains a single-stranded, inactive T7 RNA polymerase promoter sequence. The promoter is made double stranded (active) by extension (by <t>Bst</t> <t>DNA</t> polymerase) off the 3' of the extension probe, leading to the generation of large amounts of RNA signal (by T7 RNA polymerase), which may itself be amplified if required. ( b ) Detection of RNA signal by ELOSA (Enzyme Linked OligoSorbant Assay). The assay uses 2 specific probes: a biotinylated capture probe and enzyme (Alkaline phosphatase, AP) linked detection probe. Non-specific nucleic acid and 3WJ probes are removed, following binding in a streptavidin coated well, and RNA signal is detected via a colour change. Quantification of signal takes place in a 96 well plate, allowing multiple samples to be analysed simultaneously.
    Bst Dna Polymerase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst dna polymerase/product/Bio-Rad
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    99
    New England Biolabs bst 3 0 dna polymerase
    Impacts of host (human) genomic <t>DNA</t> in human haploid genome equivalents (HHGE) on specific and non-specific amplification. Plots of T m as a function TTP using Bst 2.0 at ( A ) 0 HHGE per μl; ( B ) 0.01 HHGE per μL, ( C ) 1 HHGE per μl, ( D ) 100 HHGE per μl and ( E ) 5000 HHGE per μl; and using Bst 3.0 at ( F ) 0 HHGE per μl, ( G ) 0.01 HHGE per μl, ( H ) 1 HHGE per μl, ( I ) 100 HHGE per μl ( J ) 5000 HHGE per μl in the presence of template (blue) and NTC (red). N = 3 for all conditions, except Bst 3.0 at 0 and 100 HHGE per μl in the presence of template, where N = 6.
    Bst 3 0 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst 3 0 dna polymerase/product/New England Biolabs
    Average 99 stars, based on 67 article reviews
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    bst 3 0 dna polymerase - by Bioz Stars, 2020-07
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    96
    New England Biolabs bst dna polymerase lg frag
    Impacts of host (human) genomic <t>DNA</t> in human haploid genome equivalents (HHGE) on specific and non-specific amplification. Plots of T m as a function TTP using Bst 2.0 at ( A ) 0 HHGE per μl; ( B ) 0.01 HHGE per μL, ( C ) 1 HHGE per μl, ( D ) 100 HHGE per μl and ( E ) 5000 HHGE per μl; and using Bst 3.0 at ( F ) 0 HHGE per μl, ( G ) 0.01 HHGE per μl, ( H ) 1 HHGE per μl, ( I ) 100 HHGE per μl ( J ) 5000 HHGE per μl in the presence of template (blue) and NTC (red). N = 3 for all conditions, except Bst 3.0 at 0 and 100 HHGE per μl in the presence of template, where N = 6.
    Bst Dna Polymerase Lg Frag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs bst 2 0 warmstart dna polymerase
    Species-specificity of Hha I LAMP assay. (A) Each curve represents the calculated average of triplicate turbidity curves generated with various genomic DNAs (0. 1 ng) using Bst 2.0 <t>DNA</t> polymerase without loop primers. Turbidity was observed using B. malayi or B. timori DNA. (B) As a positive control, an actin gene fragment was PCR amplified from B. malayi (Bma), D. immitis (Dim), O. volvulus (Ovo), the mosquito Aedes albopictus (Aal), W. bancrofti (Wba), human (Hsa) and B. timori (Bti) DNAs using degenerate primers. Agarose gel showing amplification of a 244 bp fragment of the actin gene. The 100 bp DNA Ladder (New England Biolabs) was used as the molecular weight marker (MWM). Water was used in the non-template controls (NTC) in (A) and (B).
    Bst 2 0 Warmstart Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 474 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst 2 0 warmstart dna polymerase/product/New England Biolabs
    Average 99 stars, based on 474 article reviews
    Price from $9.99 to $1999.99
    bst 2 0 warmstart dna polymerase - by Bioz Stars, 2020-07
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    Image Search Results


    Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the Bst DNA polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6

    Journal: Scientific Reports

    Article Title: Development of a real-time fluorescence loop-mediated isothermal amplification assay for rapid and quantitative detection of Ustilago maydis

    doi: 10.1038/s41598-017-13881-4

    Figure Lengend Snippet: Single factor experiment of RealAmp. The influence of each variable on the LAMP reaction was analyzed by the amplification curve ( a ) and the melt peak ( b ). For the Bst DNA polymerase optimization, Bst polymerase quantities were adjusted to 2 U, 4 U, 6

    Article Snippet: This optimized reaction mixture (total, 25 μl) contained 12.5 μl 2 × Bst DNA polymerase (NEB, Ipswich, MA, USA) reaction buffer [1 × containing 1.6 mM dNTPs, 1 M betaine, 4 mM MgSO4 , 20 mM Tris-HCl (pH 8.8), 10 mM KCl, 10 mM (NH4)2 SO4 , and 0.1% Triton X-20 (Sigma-Aldrich Inc., Saint Louis, USA), Double Helix Tech.

    Techniques: Amplification

    Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa DNA were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.

    Journal: PLoS ONE

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification

    doi: 10.1371/journal.pone.0139286

    Figure Lengend Snippet: Sensitivity of the L . loa RF4-based LAMP assay. Dilutions of genomic L . loa DNA were amplified with the RF4 primer set and Bst 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of L . loa DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.

    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl.

    Techniques: Lamp Assay, Amplification, Concentration Assay, Standard Deviation

    Detection of L . loa DNA in spiked blood samples. A two-fold dilution series of genomic L . loa DNA was prepared using uninfected human whole blood. NTCs only contained uninfected human whole blood. After DNA isolation, two μl of each dilution (or NTC) was used in LAMP reactions containing the V/DEF additive with Bst 2.0 DNA polymerase. For each experiment, all samples were assayed in triplicate. Average threshold times and standard deviations are plotted against ng DNA/ml of elution buffer.

    Journal: PLoS ONE

    Article Title: Genome Filtering for New DNA Biomarkers of Loa loa Infection Suitable for Loop-Mediated Isothermal Amplification

    doi: 10.1371/journal.pone.0139286

    Figure Lengend Snippet: Detection of L . loa DNA in spiked blood samples. A two-fold dilution series of genomic L . loa DNA was prepared using uninfected human whole blood. NTCs only contained uninfected human whole blood. After DNA isolation, two μl of each dilution (or NTC) was used in LAMP reactions containing the V/DEF additive with Bst 2.0 DNA polymerase. For each experiment, all samples were assayed in triplicate. Average threshold times and standard deviations are plotted against ng DNA/ml of elution buffer.

    Article Snippet: LAMP Assays LAMP reactions contained 1.6 μM each of primers FIP and BIP, 0.2 μM each of F3 and B3, 0.4 μM each of LF and FB, 1.4 mM of each dNTP, 20 mM Tris-HCl (pH 8.8), 50 mM KCl, 10 mM (NH4 )2 SO4 , 8 mM MgSO4 , 0.1% Tween-20 and 8 U of Bst 2.0 DNA Polymerase (New England Biolabs) mixed with one of several template DNAs, or 10 mM Tris-HCl (pH 8), 0.1 mM EDTA for non-template controls (NTC), in a total volume of 25 μl.

    Techniques: DNA Extraction

    Diagram illustrating the BST–DSN reaction process. ( A ) When dsDNA is used as input in a BST–DSN reaction, the nuclease DSN nicks one strand of dsDNA to create a recognition site for BST polymerase which then synthesizes a complement of the opposite DNA strand while displacing the parent strand. The displaced-sense (or anti-sense) DNA strands subsequently can re-hybridize to complementary strands and form daughter dsDNA. Subsequent DSN nicking and BST amplification generated an exponential amplification of daughter dsDNA while progressively reducing the resulting DNA size. ( B ) When single stranded DNA (ssDNA) or long oligonucleotides are used as input in BST–DSN reaction, the ssDNA is first subjected to a TdT reaction in the presence of dATP to generate a poly-A tail on the 3′ end. The unpurified TdT product is then used as input in a BST–DSN reaction in the presence of an anchored-oligo-dT which is extended by BST to create dsDNA as a first step in the reaction.

    Journal: Nucleic Acids Research

    Article Title: A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment

    doi: 10.1093/nar/gkz870

    Figure Lengend Snippet: Diagram illustrating the BST–DSN reaction process. ( A ) When dsDNA is used as input in a BST–DSN reaction, the nuclease DSN nicks one strand of dsDNA to create a recognition site for BST polymerase which then synthesizes a complement of the opposite DNA strand while displacing the parent strand. The displaced-sense (or anti-sense) DNA strands subsequently can re-hybridize to complementary strands and form daughter dsDNA. Subsequent DSN nicking and BST amplification generated an exponential amplification of daughter dsDNA while progressively reducing the resulting DNA size. ( B ) When single stranded DNA (ssDNA) or long oligonucleotides are used as input in BST–DSN reaction, the ssDNA is first subjected to a TdT reaction in the presence of dATP to generate a poly-A tail on the 3′ end. The unpurified TdT product is then used as input in a BST–DSN reaction in the presence of an anchored-oligo-dT which is extended by BST to create dsDNA as a first step in the reaction.

    Article Snippet: BST–DSN reaction using PCR products as input BST 2.0 DNA polymerase (BST) and DSN were purchased from NEB and Sapphire North America, respectively.

    Techniques: Amplification, Generated

    The SMART assay. ( a ) Specific probes hybridise with the target to form a three-way junction (3WJ), assisted by facilitator probes (f1 f2). The 3WJ initially contains a single-stranded, inactive T7 RNA polymerase promoter sequence. The promoter is made double stranded (active) by extension (by Bst DNA polymerase) off the 3' of the extension probe, leading to the generation of large amounts of RNA signal (by T7 RNA polymerase), which may itself be amplified if required. ( b ) Detection of RNA signal by ELOSA (Enzyme Linked OligoSorbant Assay). The assay uses 2 specific probes: a biotinylated capture probe and enzyme (Alkaline phosphatase, AP) linked detection probe. Non-specific nucleic acid and 3WJ probes are removed, following binding in a streptavidin coated well, and RNA signal is detected via a colour change. Quantification of signal takes place in a 96 well plate, allowing multiple samples to be analysed simultaneously.

    Journal: Virology Journal

    Article Title: Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp

    doi: 10.1186/1743-422X-4-52

    Figure Lengend Snippet: The SMART assay. ( a ) Specific probes hybridise with the target to form a three-way junction (3WJ), assisted by facilitator probes (f1 f2). The 3WJ initially contains a single-stranded, inactive T7 RNA polymerase promoter sequence. The promoter is made double stranded (active) by extension (by Bst DNA polymerase) off the 3' of the extension probe, leading to the generation of large amounts of RNA signal (by T7 RNA polymerase), which may itself be amplified if required. ( b ) Detection of RNA signal by ELOSA (Enzyme Linked OligoSorbant Assay). The assay uses 2 specific probes: a biotinylated capture probe and enzyme (Alkaline phosphatase, AP) linked detection probe. Non-specific nucleic acid and 3WJ probes are removed, following binding in a streptavidin coated well, and RNA signal is detected via a colour change. Quantification of signal takes place in a 96 well plate, allowing multiple samples to be analysed simultaneously.

    Article Snippet: Samples were mixed, heated at 90°C for 3 min on a PTC-200™ thermal cycler (MJ Research, Waltham, MA, USA), ramped down to 41°C (0.1°C/s) and held at this temperature for 1 h. A 5 μL volume of solution containing dNTPs (5 μM each), NTPs (2 mM each) (both from Amersham Biosciences, Aylesbury UK), 4 U Bst (3' to 5'exo- ) DNA polymerase (New England Biolabs, Beverly, MA, USA) and 240 U T7 RNA polymerase (Ambion) was then added, and the reaction was incubated at 41°C for an additional 2 h. To amplify the RNA signal further, the samples were brought to room temperature before the addition of 20 fmol RNA amplification probe, followed by a mixture containing 4.5 μL 10× transcription buffer, dNTPs (50 μM each dNTP), NTPs (2 mM each NTP), 4 U Bst (3' to 5'exo- ) DNA polymerase, 160 U T7 RNA polymerase, and ultra-pure, sterile, RNAse-free water to give a final volume of 17 μL.

    Techniques: Sequencing, Amplification, Binding Assay

    Impacts of host (human) genomic DNA in human haploid genome equivalents (HHGE) on specific and non-specific amplification. Plots of T m as a function TTP using Bst 2.0 at ( A ) 0 HHGE per μl; ( B ) 0.01 HHGE per μL, ( C ) 1 HHGE per μl, ( D ) 100 HHGE per μl and ( E ) 5000 HHGE per μl; and using Bst 3.0 at ( F ) 0 HHGE per μl, ( G ) 0.01 HHGE per μl, ( H ) 1 HHGE per μl, ( I ) 100 HHGE per μl ( J ) 5000 HHGE per μl in the presence of template (blue) and NTC (red). N = 3 for all conditions, except Bst 3.0 at 0 and 100 HHGE per μl in the presence of template, where N = 6.

    Journal: Nucleic Acids Research

    Article Title: Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification

    doi: 10.1093/nar/gkaa099

    Figure Lengend Snippet: Impacts of host (human) genomic DNA in human haploid genome equivalents (HHGE) on specific and non-specific amplification. Plots of T m as a function TTP using Bst 2.0 at ( A ) 0 HHGE per μl; ( B ) 0.01 HHGE per μL, ( C ) 1 HHGE per μl, ( D ) 100 HHGE per μl and ( E ) 5000 HHGE per μl; and using Bst 3.0 at ( F ) 0 HHGE per μl, ( G ) 0.01 HHGE per μl, ( H ) 1 HHGE per μl, ( I ) 100 HHGE per μl ( J ) 5000 HHGE per μl in the presence of template (blue) and NTC (red). N = 3 for all conditions, except Bst 3.0 at 0 and 100 HHGE per μl in the presence of template, where N = 6.

    Article Snippet: LAMP reagents IsoAmp I (#B0537S), IsoAmp II (#B0374S), MgSO4 (#B1003S), deoxynucleotide solution (#N0447S), Bovine Serum Albumen (BSA, #B9000S0), Bst 2.0 (8,000 U/ml, #M0537S) and Bst 3.0 (8000 U/ml, #M0374S) were purchased from New England Biolabs (Ipswich, MA, USA).

    Techniques: Amplification

    Species-specificity of Hha I LAMP assay. (A) Each curve represents the calculated average of triplicate turbidity curves generated with various genomic DNAs (0. 1 ng) using Bst 2.0 DNA polymerase without loop primers. Turbidity was observed using B. malayi or B. timori DNA. (B) As a positive control, an actin gene fragment was PCR amplified from B. malayi (Bma), D. immitis (Dim), O. volvulus (Ovo), the mosquito Aedes albopictus (Aal), W. bancrofti (Wba), human (Hsa) and B. timori (Bti) DNAs using degenerate primers. Agarose gel showing amplification of a 244 bp fragment of the actin gene. The 100 bp DNA Ladder (New England Biolabs) was used as the molecular weight marker (MWM). Water was used in the non-template controls (NTC) in (A) and (B).

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Diagnosis of Brugian Filariasis by Loop-Mediated Isothermal Amplification

    doi: 10.1371/journal.pntd.0001948

    Figure Lengend Snippet: Species-specificity of Hha I LAMP assay. (A) Each curve represents the calculated average of triplicate turbidity curves generated with various genomic DNAs (0. 1 ng) using Bst 2.0 DNA polymerase without loop primers. Turbidity was observed using B. malayi or B. timori DNA. (B) As a positive control, an actin gene fragment was PCR amplified from B. malayi (Bma), D. immitis (Dim), O. volvulus (Ovo), the mosquito Aedes albopictus (Aal), W. bancrofti (Wba), human (Hsa) and B. timori (Bti) DNAs using degenerate primers. Agarose gel showing amplification of a 244 bp fragment of the actin gene. The 100 bp DNA Ladder (New England Biolabs) was used as the molecular weight marker (MWM). Water was used in the non-template controls (NTC) in (A) and (B).

    Article Snippet: Reaction times were slightly slower using Bst 2.0 WarmStart DNA polymerase regardless of the presence of loop primers ( ).

    Techniques: Lamp Assay, Generated, Positive Control, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Molecular Weight, Marker

    Sensitivity of Hha I LAMP assay. Ten-fold serial dilutions of B. malayi genomic DNA amplified with the Hha I primer set alone (A) or in the presence of loop primers (B) with Bst DNA polymerase, large fragment (wt Bst LF), Bst 2.0 DNA polymerase ( Bst 2.0) and Bst 2.0 WarmStart DNA polymerase ( Bst 2.0 WS). Data points represent the average of three samples and the error bars represent the standard deviation at each point. For each enzyme, the average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the amount of starting material. (C) UV detection (365 nm) of products generated within 60 minutes using Bst 2.0 in the presence of loop primers and Fluorescent Detection Reagent. The amount of starting material in ng is shown below the photograph. Positive samples fluoresce green while negative samples remain dark.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Diagnosis of Brugian Filariasis by Loop-Mediated Isothermal Amplification

    doi: 10.1371/journal.pntd.0001948

    Figure Lengend Snippet: Sensitivity of Hha I LAMP assay. Ten-fold serial dilutions of B. malayi genomic DNA amplified with the Hha I primer set alone (A) or in the presence of loop primers (B) with Bst DNA polymerase, large fragment (wt Bst LF), Bst 2.0 DNA polymerase ( Bst 2.0) and Bst 2.0 WarmStart DNA polymerase ( Bst 2.0 WS). Data points represent the average of three samples and the error bars represent the standard deviation at each point. For each enzyme, the average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the amount of starting material. (C) UV detection (365 nm) of products generated within 60 minutes using Bst 2.0 in the presence of loop primers and Fluorescent Detection Reagent. The amount of starting material in ng is shown below the photograph. Positive samples fluoresce green while negative samples remain dark.

    Article Snippet: Reaction times were slightly slower using Bst 2.0 WarmStart DNA polymerase regardless of the presence of loop primers ( ).

    Techniques: Lamp Assay, Amplification, Standard Deviation, Generated

    Hha I LAMP assay for the detection of B. malayi infected blood samples. A set of serial dilutions (two-fold) of microfilariae in blood was prepared and DNA was isolated from each dilution. Three experiments were performed using a different but overlapping range of DNA dilutions. One µl of DNA from each dilution was used in LAMP reactions with Bst 2.0 DNA polymerase. Samples from each experimental set-up were performed in triplicate (experiments 1 and 2) or duplicate (experiment 3). Average threshold times and standard deviations were plotted against the approximate number of mf/µl DNA solution.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Diagnosis of Brugian Filariasis by Loop-Mediated Isothermal Amplification

    doi: 10.1371/journal.pntd.0001948

    Figure Lengend Snippet: Hha I LAMP assay for the detection of B. malayi infected blood samples. A set of serial dilutions (two-fold) of microfilariae in blood was prepared and DNA was isolated from each dilution. Three experiments were performed using a different but overlapping range of DNA dilutions. One µl of DNA from each dilution was used in LAMP reactions with Bst 2.0 DNA polymerase. Samples from each experimental set-up were performed in triplicate (experiments 1 and 2) or duplicate (experiment 3). Average threshold times and standard deviations were plotted against the approximate number of mf/µl DNA solution.

    Article Snippet: Reaction times were slightly slower using Bst 2.0 WarmStart DNA polymerase regardless of the presence of loop primers ( ).

    Techniques: Lamp Assay, Infection, Isolation