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  • 95
    New England Biolabs bss hii
    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), <t>Bss</t> <t>HII</t> (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Bss Hii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bss hii/product/New England Biolabs
    Average 95 stars, based on 285 article reviews
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    89
    Roche bss hii
    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), <t>Bss</t> <t>HII</t> (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Bss Hii, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 7 article reviews
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    89
    Promega bss hii
    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), <t>Bss</t> <t>HII</t> (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Bss Hii, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bss hii/product/Promega
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    bss hii - by Bioz Stars, 2020-08
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    86
    TaKaRa bss hii restriction enzyme
    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), <t>Bss</t> <t>HII</t> (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Bss Hii Restriction Enzyme, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Boehringer Mannheim bss hii
    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), <t>Bss</t> <t>HII</t> (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).
    Bss Hii, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 89/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bss hii/product/Boehringer Mannheim
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    94
    TaKaRa bss hii
    Sequence-specific protection of pSPORT I plasmid DNA from restriction endonuclease <t>Bss</t> <t>HII</t> hydrolysis by incubation with ImPyImLDu86 ( 7 ). ( A ) A Bss HII restriction map of pSPORT I DNA and schematic representation of the binding mode of conjugate 7 at Bss HII hydrolysis sites. The Py and Im rings are represented by open and closed circles, respectively; the triangle represents segment A of DU-86. ( B ) Agarose gel (1.2%) electrophoresis of triamide–CPI conjugate-treated pSPORT I DNA after Bss HII (2.5 U) digestion (lanes 3–12). Lane 1, 250-bp DNA marker; lane 2, pSPORT I DNA control; lane 3, no drug treatment; lanes 4–11, 5 µM, 1 µM, 500 nM, 250 nM, 100 nM, 50 nM, 10 nM, 1 nM concentrations of conjugate 7 ; lane 12, 5 µM conjugate 14 . sc, supercoiled DNA; li, linear DNA; oc, open circle DNA.
    Bss Hii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs endonuclease bss hii
    Sequence-specific protection of pSPORT I plasmid DNA from restriction endonuclease <t>Bss</t> <t>HII</t> hydrolysis by incubation with ImPyImLDu86 ( 7 ). ( A ) A Bss HII restriction map of pSPORT I DNA and schematic representation of the binding mode of conjugate 7 at Bss HII hydrolysis sites. The Py and Im rings are represented by open and closed circles, respectively; the triangle represents segment A of DU-86. ( B ) Agarose gel (1.2%) electrophoresis of triamide–CPI conjugate-treated pSPORT I DNA after Bss HII (2.5 U) digestion (lanes 3–12). Lane 1, 250-bp DNA marker; lane 2, pSPORT I DNA control; lane 3, no drug treatment; lanes 4–11, 5 µM, 1 µM, 500 nM, 250 nM, 100 nM, 50 nM, 10 nM, 1 nM concentrations of conjugate 7 ; lane 12, 5 µM conjugate 14 . sc, supercoiled DNA; li, linear DNA; oc, open circle DNA.
    Endonuclease Bss Hii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    doi:

    Figure Lengend Snippet: DNA mapping of pBluMH. ( A ) Molecular mass markers (lanes 1 and 7). pBluMH digested with Pst I (lane 2), Eag I (lane 3), Bss HII (lane 4), Eag I + Pst I (lane 5), and Bss HII + Pst I (lane 6). ( B ) Southern hybridization of the gel in A . pBluMH digested with Bss HII + Pst I (lane 1), Eag I + Pst I (lane 2), Bss HII (lane 3), Eag I (lane 4), Pst I (lane 5), and DNA molecular mass markers (lane 6).

    Article Snippet: According to the pBluescript map, we would expect to see the DNA fragment next to the pBluescript vector at the T3 promoter site in pBluMH to be about 0.1 kb smaller when double-digested with Bss HII + Pst I than when digested with Bss HII alone.

    Techniques: Hybridization

    Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

    doi:

    Figure Lengend Snippet: Location of restriction enzyme Eag I and Bss HII sites in the probe sequence. The Eag I site is located 66 nucleotides and Bss HII site is located 192 nucleotides from the 5′ end. The mark indicates the length representing 75 nucleotides.

    Article Snippet: According to the pBluescript map, we would expect to see the DNA fragment next to the pBluescript vector at the T3 promoter site in pBluMH to be about 0.1 kb smaller when double-digested with Bss HII + Pst I than when digested with Bss HII alone.

    Techniques: Sequencing

    Sequence-specific protection of pSPORT I plasmid DNA from restriction endonuclease Bss HII hydrolysis by incubation with ImPyImLDu86 ( 7 ). ( A ) A Bss HII restriction map of pSPORT I DNA and schematic representation of the binding mode of conjugate 7 at Bss HII hydrolysis sites. The Py and Im rings are represented by open and closed circles, respectively; the triangle represents segment A of DU-86. ( B ) Agarose gel (1.2%) electrophoresis of triamide–CPI conjugate-treated pSPORT I DNA after Bss HII (2.5 U) digestion (lanes 3–12). Lane 1, 250-bp DNA marker; lane 2, pSPORT I DNA control; lane 3, no drug treatment; lanes 4–11, 5 µM, 1 µM, 500 nM, 250 nM, 100 nM, 50 nM, 10 nM, 1 nM concentrations of conjugate 7 ; lane 12, 5 µM conjugate 14 . sc, supercoiled DNA; li, linear DNA; oc, open circle DNA.

    Journal: Nucleic Acids Research

    Article Title: Sequence-specific protection of plasmid DNA from restriction endonuclease hydrolysis by pyrrole-imidazole-cyclopropapyrroloindole conjugates

    doi:

    Figure Lengend Snippet: Sequence-specific protection of pSPORT I plasmid DNA from restriction endonuclease Bss HII hydrolysis by incubation with ImPyImLDu86 ( 7 ). ( A ) A Bss HII restriction map of pSPORT I DNA and schematic representation of the binding mode of conjugate 7 at Bss HII hydrolysis sites. The Py and Im rings are represented by open and closed circles, respectively; the triangle represents segment A of DU-86. ( B ) Agarose gel (1.2%) electrophoresis of triamide–CPI conjugate-treated pSPORT I DNA after Bss HII (2.5 U) digestion (lanes 3–12). Lane 1, 250-bp DNA marker; lane 2, pSPORT I DNA control; lane 3, no drug treatment; lanes 4–11, 5 µM, 1 µM, 500 nM, 250 nM, 100 nM, 50 nM, 10 nM, 1 nM concentrations of conjugate 7 ; lane 12, 5 µM conjugate 14 . sc, supercoiled DNA; li, linear DNA; oc, open circle DNA.

    Article Snippet: Restriction endonucleases, Bss HII, Eco 52I, Dra I and Hin dIII were purchased from Takara Shuzo Co. Ltd. Syntheses of Py–Im–CPI conjugates ImPyImLDu86 ( 7 ) and ImImPyLDu86 ( 14 ) are described in the Supplementary Material.

    Techniques: Sequencing, Plasmid Preparation, Incubation, Binding Assay, Agarose Gel Electrophoresis, Electrophoresis, Marker