bsrgi Thermo Fisher Search Results


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  • 89
    Thermo Fisher bsp1407i
    Bsp1407i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher restriction enzyme bsrgi
    Restriction Enzyme Bsrgi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rnaseh
    Rnaseh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher ecor i
    Ecor I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher restriction enzyme pfimi
    Restriction Enzyme Pfimi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    70
    Thermo Fisher china bse8i
    China Bse8i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher csp6i
    Csp6i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher actev protease
    Actev Protease, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rnase inhibitor
    Rnase Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher dpn i
    Dpn I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    70
    Thermo Fisher esp3i bsmbi 10 u µl
    Esp3i Bsmbi 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dnase
    Dnase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14041 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher taq polymerase
    Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23464 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher psp1406i
    A , Genomic DNA from some adult male H. contortus were amplified to obtain PCR products of 403bp. B1 , In order to create recognition site for PSP1406 I, semi nested PCR technique was performed, using UT vet MF-primer and reverse primer. All lanes are the representative PCR products with 222bp in length. B2 , 222bp PCR products were cut with the <t>PSP1406I.</t> First lane is uncut control sample and all lanes showed BZss homozygote. B3 , A semi nested PCR was performed on first 403bp PCR products in order to use another restriction enzyme TaaI. B4 , 225bp PCR products were digested by TaaI. First lane is first 403bp uncut control and second lane is 403bp product cut with TaaI and two fragments with 245 and 158 bp are detectable. All lanes didn't cut with enzyme and showed BZss homozygote. In all A, B1, B2, B3, B4 M is 100bp marker
    Psp1406i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher taai
    A Genomic DNA of each single H. contortus was amplified with P1 and P2 primers to obtain a <t>PCR</t> product of 527 bp (Lane 1 and 3). Subsequently, each PCR product was amplified with UT-HC167 MF-primer and P2 primer to obtain a PCR product of 451 bp containing codons 167 and 200 (Lane 2 and 4). B The second PCR products were digested with SnaB I. The products could not be cut with this enzyme (BZ SS ; Lane 1, 2 and 3). To control the activity of enzyme, the positive control template (243 bp; Lane 5) was cut into two fragments of 200 and 43 bp (Lane 4). C The second PCR products (451 bp; Lane 1) were also digested with <t>TaaI.</t> The products were cut into two fragments of 207 and 244 bp (BZ SS ; Lane 2, 3 and 4). M is 100 bp marker
    Taai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher dna pol i
    A Genomic DNA of each single H. contortus was amplified with P1 and P2 primers to obtain a <t>PCR</t> product of 527 bp (Lane 1 and 3). Subsequently, each PCR product was amplified with UT-HC167 MF-primer and P2 primer to obtain a PCR product of 451 bp containing codons 167 and 200 (Lane 2 and 4). B The second PCR products were digested with SnaB I. The products could not be cut with this enzyme (BZ SS ; Lane 1, 2 and 3). To control the activity of enzyme, the positive control template (243 bp; Lane 5) was cut into two fragments of 200 and 43 bp (Lane 4). C The second PCR products (451 bp; Lane 1) were also digested with <t>TaaI.</t> The products were cut into two fragments of 207 and 244 bp (BZ SS ; Lane 2, 3 and 4). M is 100 bp marker
    Dna Pol I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher t4 rna ligase
    A Genomic DNA of each single H. contortus was amplified with P1 and P2 primers to obtain a <t>PCR</t> product of 527 bp (Lane 1 and 3). Subsequently, each PCR product was amplified with UT-HC167 MF-primer and P2 primer to obtain a PCR product of 451 bp containing codons 167 and 200 (Lane 2 and 4). B The second PCR products were digested with SnaB I. The products could not be cut with this enzyme (BZ SS ; Lane 1, 2 and 3). To control the activity of enzyme, the positive control template (243 bp; Lane 5) was cut into two fragments of 200 and 43 bp (Lane 4). C The second PCR products (451 bp; Lane 1) were also digested with <t>TaaI.</t> The products were cut into two fragments of 207 and 244 bp (BZ SS ; Lane 2, 3 and 4). M is 100 bp marker
    T4 Rna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 2326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    70
    Thermo Fisher bpii bbsi 10 u µl
    A Genomic DNA of each single H. contortus was amplified with P1 and P2 primers to obtain a <t>PCR</t> product of 527 bp (Lane 1 and 3). Subsequently, each PCR product was amplified with UT-HC167 MF-primer and P2 primer to obtain a PCR product of 451 bp containing codons 167 and 200 (Lane 2 and 4). B The second PCR products were digested with SnaB I. The products could not be cut with this enzyme (BZ SS ; Lane 1, 2 and 3). To control the activity of enzyme, the positive control template (243 bp; Lane 5) was cut into two fragments of 200 and 43 bp (Lane 4). C The second PCR products (451 bp; Lane 1) were also digested with <t>TaaI.</t> The products were cut into two fragments of 207 and 244 bp (BZ SS ; Lane 2, 3 and 4). M is 100 bp marker
    Bpii Bbsi 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher t4 polynucleotide kinase
    A Genomic DNA of each single H. contortus was amplified with P1 and P2 primers to obtain a <t>PCR</t> product of 527 bp (Lane 1 and 3). Subsequently, each PCR product was amplified with UT-HC167 MF-primer and P2 primer to obtain a PCR product of 451 bp containing codons 167 and 200 (Lane 2 and 4). B The second PCR products were digested with SnaB I. The products could not be cut with this enzyme (BZ SS ; Lane 1, 2 and 3). To control the activity of enzyme, the positive control template (243 bp; Lane 5) was cut into two fragments of 200 and 43 bp (Lane 4). C The second PCR products (451 bp; Lane 1) were also digested with <t>TaaI.</t> The products were cut into two fragments of 207 and 244 bp (BZ SS ; Lane 2, 3 and 4). M is 100 bp marker
    T4 Polynucleotide Kinase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    70
    Thermo Fisher psti 10 u µl
    A Genomic DNA of each single H. contortus was amplified with P1 and P2 primers to obtain a <t>PCR</t> product of 527 bp (Lane 1 and 3). Subsequently, each PCR product was amplified with UT-HC167 MF-primer and P2 primer to obtain a PCR product of 451 bp containing codons 167 and 200 (Lane 2 and 4). B The second PCR products were digested with SnaB I. The products could not be cut with this enzyme (BZ SS ; Lane 1, 2 and 3). To control the activity of enzyme, the positive control template (243 bp; Lane 5) was cut into two fragments of 200 and 43 bp (Lane 4). C The second PCR products (451 bp; Lane 1) were also digested with <t>TaaI.</t> The products were cut into two fragments of 207 and 244 bp (BZ SS ; Lane 2, 3 and 4). M is 100 bp marker
    Psti 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dpni
    A Genomic DNA of each single H. contortus was amplified with P1 and P2 primers to obtain a <t>PCR</t> product of 527 bp (Lane 1 and 3). Subsequently, each PCR product was amplified with UT-HC167 MF-primer and P2 primer to obtain a PCR product of 451 bp containing codons 167 and 200 (Lane 2 and 4). B The second PCR products were digested with SnaB I. The products could not be cut with this enzyme (BZ SS ; Lane 1, 2 and 3). To control the activity of enzyme, the positive control template (243 bp; Lane 5) was cut into two fragments of 200 and 43 bp (Lane 4). C The second PCR products (451 bp; Lane 1) were also digested with <t>TaaI.</t> The products were cut into two fragments of 207 and 244 bp (BZ SS ; Lane 2, 3 and 4). M is 100 bp marker
    Dpni, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 952 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pfu dna polymerase
    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using <t>Pfu</t> <t>DNA</t> polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.
    Pfu Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taq dna polymerase
    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using <t>Pfu</t> <t>DNA</t> polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dna polymerase i
    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using <t>Pfu</t> <t>DNA</t> polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.
    Dna Polymerase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1883 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase t1
    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using <t>Pfu</t> <t>DNA</t> polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.
    Rnase T1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2881 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    70
    Thermo Fisher rnase i 10 u µl
    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using <t>Pfu</t> <t>DNA</t> polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.
    Rnase I 10 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnase h
    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using <t>Pfu</t> <t>DNA</t> polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.
    Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7011 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher e coli dna ligase
    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using <t>Pfu</t> <t>DNA</t> polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.
    E Coli Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A , Genomic DNA from some adult male H. contortus were amplified to obtain PCR products of 403bp. B1 , In order to create recognition site for PSP1406 I, semi nested PCR technique was performed, using UT vet MF-primer and reverse primer. All lanes are the representative PCR products with 222bp in length. B2 , 222bp PCR products were cut with the PSP1406I. First lane is uncut control sample and all lanes showed BZss homozygote. B3 , A semi nested PCR was performed on first 403bp PCR products in order to use another restriction enzyme TaaI. B4 , 225bp PCR products were digested by TaaI. First lane is first 403bp uncut control and second lane is 403bp product cut with TaaI and two fragments with 245 and 158 bp are detectable. All lanes didn't cut with enzyme and showed BZss homozygote. In all A, B1, B2, B3, B4 M is 100bp marker

    Journal: Iranian Journal of Parasitology

    Article Title: Evaluation of Benzimidazole Resistance in Haemonchus contortus Using Comparative PCR-RFLP Methods

    doi:

    Figure Lengend Snippet: A , Genomic DNA from some adult male H. contortus were amplified to obtain PCR products of 403bp. B1 , In order to create recognition site for PSP1406 I, semi nested PCR technique was performed, using UT vet MF-primer and reverse primer. All lanes are the representative PCR products with 222bp in length. B2 , 222bp PCR products were cut with the PSP1406I. First lane is uncut control sample and all lanes showed BZss homozygote. B3 , A semi nested PCR was performed on first 403bp PCR products in order to use another restriction enzyme TaaI. B4 , 225bp PCR products were digested by TaaI. First lane is first 403bp uncut control and second lane is 403bp product cut with TaaI and two fragments with 245 and 158 bp are detectable. All lanes didn't cut with enzyme and showed BZss homozygote. In all A, B1, B2, B3, B4 M is 100bp marker

    Article Snippet: Ten µl of each resulting semi nested PCR product was digested by 2 µl PSP1406I (Fermenta,10 U/ µl) in 2 µl 10× buffer Tango and 18 µl nuclease-free water for 16 h at 37°C.

    Techniques: Amplification, Polymerase Chain Reaction, Nested PCR, Marker

    A Genomic DNA of each single H. contortus was amplified with P1 and P2 primers to obtain a PCR product of 527 bp (Lane 1 and 3). Subsequently, each PCR product was amplified with UT-HC167 MF-primer and P2 primer to obtain a PCR product of 451 bp containing codons 167 and 200 (Lane 2 and 4). B The second PCR products were digested with SnaB I. The products could not be cut with this enzyme (BZ SS ; Lane 1, 2 and 3). To control the activity of enzyme, the positive control template (243 bp; Lane 5) was cut into two fragments of 200 and 43 bp (Lane 4). C The second PCR products (451 bp; Lane 1) were also digested with TaaI. The products were cut into two fragments of 207 and 244 bp (BZ SS ; Lane 2, 3 and 4). M is 100 bp marker

    Journal: Iranian Journal of Parasitology

    Article Title: Benzimidazole -Resistance in Haemonchus contortus: New PCR-RFLP Method for the Detection of Point Mutation at Codon 167 of Isotype 1 ?-Tubulin Gene

    doi:

    Figure Lengend Snippet: A Genomic DNA of each single H. contortus was amplified with P1 and P2 primers to obtain a PCR product of 527 bp (Lane 1 and 3). Subsequently, each PCR product was amplified with UT-HC167 MF-primer and P2 primer to obtain a PCR product of 451 bp containing codons 167 and 200 (Lane 2 and 4). B The second PCR products were digested with SnaB I. The products could not be cut with this enzyme (BZ SS ; Lane 1, 2 and 3). To control the activity of enzyme, the positive control template (243 bp; Lane 5) was cut into two fragments of 200 and 43 bp (Lane 4). C The second PCR products (451 bp; Lane 1) were also digested with TaaI. The products were cut into two fragments of 207 and 244 bp (BZ SS ; Lane 2, 3 and 4). M is 100 bp marker

    Article Snippet: Detection of point mutation at codon 200 The PCR products (451 bp) were also digested with TaaI (Fermenta, 10 U/µl) to analyze the SNP at codon 200 ( ).

    Techniques: Amplification, Polymerase Chain Reaction, Activity Assay, Positive Control, Marker

    Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using Pfu DNA polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.

    Journal: Avicenna Journal of Medical Biotechnology

    Article Title: Cloning and Expression of CD19, a Human B-Cell Marker in NIH-3T3 Cell Line

    doi:

    Figure Lengend Snippet: Cloning and sub-cloning of CD19 cDNA. A) Amplification of specific band for human CD19 cDNA using Pfu DNA polymerase; B) Colony-PCR reaction on eight white colonies (1-8) after blue/ white selection. C) Excision of 1701 bp band for human CD19 cDNA after double digestion of the construct using KpnI and HindIII restriction enzymes. Lanes (a) and (a’): undigested pGEMT-easy/CD19 construct, Lanes (b) partial and complete digestion by KpnI and Hind III, respectively and (b’): complete digestion by both KpnI and HindIII. SM: DNA size marker ( bp ). Asterisks (*) point the desired band.

    Article Snippet: For amplification of CD19 cDNA, PCR was performed in a 25 µl reaction solution contained 2.5 µl 10×PCR buffer, 1.5 µl 10 mM dNTPs, 1 µl of each primer (10 pmol/µl ), 0.5 µl Pfu DNA polymerase (10 U/µl ) (Thermo Fisher Scientific, Inc., MA, USA), 1 mM MgSO4 and 1 µl cDNA.

    Techniques: Clone Assay, Subcloning, Amplification, Polymerase Chain Reaction, Selection, Construct, Marker