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  • 95
    Qiagen minelute pcr purification kit
    Minelute Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 16148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 16148 article reviews
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    minelute pcr purification kit - by Bioz Stars, 2020-02
    95/100 stars
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    94
    TaKaRa bspei
    Bspei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 138 article reviews
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    bspei - by Bioz Stars, 2020-02
    94/100 stars
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    78
    TaKaRa clai bspei digested pirespuro2
    Clai Bspei Digested Pirespuro2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 6 article reviews
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    79
    TaKaRa nhei bspei egfp cdna fragment
    Nhei Bspei Egfp Cdna Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa bspei kpni digested pegfp c3 vector
    Bspei Kpni Digested Pegfp C3 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 9 article reviews
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    bspei kpni digested pegfp c3 vector - by Bioz Stars, 2020-02
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    78
    TaKaRa bspe1
    Bspe1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 7 article reviews
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    bspe1 - by Bioz Stars, 2020-02
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    96
    TaKaRa pegfp ci
    Pegfp Ci, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pecfp c1
    Pecfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pegfp n1
    Pegfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14657 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pegfp c1
    VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. <t>peGFP-C1</t> (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).
    Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 880 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa full length cdna
    VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. <t>peGFP-C1</t> (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).
    Full Length Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa puromycin dependent expression
    VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. <t>peGFP-C1</t> (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).
    Puromycin Dependent Expression, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    TaKaRa subcloning
    VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. <t>peGFP-C1</t> (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).
    Subcloning, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    TaKaRa pires puro3
    VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. <t>peGFP-C1</t> (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).
    Pires Puro3, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pmcherry c1
    VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. <t>peGFP-C1</t> (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).
    Pmcherry C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    TaKaRa plasmid pecfp c1
    VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. <t>peGFP-C1</t> (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).
    Plasmid Pecfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pegfp c1 plasmid
    VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. <t>peGFP-C1</t> (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).
    Pegfp C1 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1839 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    TaKaRa human α tubulin
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
    Human α Tubulin, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pegfp 1
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
    Pegfp 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nhei  (TaKaRa)
    90
    TaKaRa nhei
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
    Nhei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa peyfp n1
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
    Peyfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa bglii sali sites
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
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    TaKaRa asei klenow ngomiv cut ptuner ires2
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
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    TaKaRa peyfp c1
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
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    TaKaRa plasmid pegfp
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
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    TaKaRa pegfp c1 clontech plasmid
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
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    TaKaRa egfp sequence
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
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    TaKaRa primer
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
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    TaKaRa ptre2 vector
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
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    TaKaRa bglii
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
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    87
    TaKaRa pecfp 1
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
    Pecfp 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    TaKaRa egfp cassette
    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) <t>mCerulean3-α-tubulin-6</t> (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.
    Egfp Cassette, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. peGFP-C1 (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).

    Journal: eLife

    Article Title: Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility

    doi: 10.7554/eLife.43561

    Figure Lengend Snippet: VPS13A interacts with VAP-A in human cells. ( A ) GFP-VPS13A constructs presented in Figure 2D–E were overexpressed in HEK293T cells for 24 hr. Cell lysates were processed for immunoblot analysis using an antibody against GFP. peGFP-C1 (GFP) expressing cells were used as a control. The stain free gel is shown as a loading control. B,C GFP-VPS13 (2003–2606) and GFP-VPS13A (2615–3174) constructs were expressed in either HEK293T cells (B/B’) or U2OS cells (C/C’). Note the differences in mitochondria morphology in both cell types. ( D ) U2OS cells expressing GFP-VPS13A (2615–3174) for 24 hr were stained with Mitotracker Red. D’ shows a higher magnification of the insert in D. Cells were co-transfected with mCherrySec61 (B/C) and BFP-Sec61 (D/D’) (not shown). Scale bars = 25 μm ( B–C’ ) and 10 μm ( D–D’ ).

    Article Snippet: To obtain GFP-VAP-A, VAP-A was amplified by PCR from the His-VAP-A plasmid and inserted into pEGFP-C1 (Clontech) via EcoRI and BamHI restriction sites.

    Techniques: Construct, Expressing, Staining, Transfection

    VPS13A colocalizes with mitochondria but not with the endocytic compartment. ( A ) 48 hours after transfection with either VPS13A-Myc or VPS13A-GFP, HEK293T cells were processed for immunoblotting using antibodies against VPS13A and a -Tubulin. peGFP-C1 transfected or non-transfected (NT) cells were used as controls. Note the enrichment of VPS13A in both VPS13A-Myc or VPS13A-GFP lanes. ( B ) Quantification of protein bands detected with anti-VPS13A antibody in A. The ratio of VPS13A to a -tubulin was normalized to NT cells. Error bars, mean ± s.e.m (n=3), two-tailed unpaired Student’s t-test was used (*P ≤ 0.05, **P≤0.01). C-F HEK293T cells were co-transfected with VPS13A-Myc and with GFP-Rab5 Q79L ( C ), GFP-Rab7 Q67L ( D ), LAMP1-GFP ( E ) or mCherry FYCO1 ( F ). Cells were stained with anti-myc (C-E, red; F, green) and DAPI (blue). Bottom panels (C’-F’ show a magnification of the inset in top panels. Scale bars = 10 µm ( C–F’ ).

    Journal: eLife

    Article Title: Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility

    doi: 10.7554/eLife.43561

    Figure Lengend Snippet: VPS13A colocalizes with mitochondria but not with the endocytic compartment. ( A ) 48 hours after transfection with either VPS13A-Myc or VPS13A-GFP, HEK293T cells were processed for immunoblotting using antibodies against VPS13A and a -Tubulin. peGFP-C1 transfected or non-transfected (NT) cells were used as controls. Note the enrichment of VPS13A in both VPS13A-Myc or VPS13A-GFP lanes. ( B ) Quantification of protein bands detected with anti-VPS13A antibody in A. The ratio of VPS13A to a -tubulin was normalized to NT cells. Error bars, mean ± s.e.m (n=3), two-tailed unpaired Student’s t-test was used (*P ≤ 0.05, **P≤0.01). C-F HEK293T cells were co-transfected with VPS13A-Myc and with GFP-Rab5 Q79L ( C ), GFP-Rab7 Q67L ( D ), LAMP1-GFP ( E ) or mCherry FYCO1 ( F ). Cells were stained with anti-myc (C-E, red; F, green) and DAPI (blue). Bottom panels (C’-F’ show a magnification of the inset in top panels. Scale bars = 10 µm ( C–F’ ).

    Article Snippet: To obtain GFP-VAP-A, VAP-A was amplified by PCR from the His-VAP-A plasmid and inserted into pEGFP-C1 (Clontech) via EcoRI and BamHI restriction sites.

    Techniques: Transfection, Two Tailed Test, Staining

    VPS13A interacts with VAP-A. ( A ) GST-fusion proteins of VPS13A fragments expressed in E.Coli were enriched on Sepharose beads and incubated with equal amounts of HeLa cell lysate. GST alone was used as a control. Samples were immunoblotted against VAP-A, GST and N-terminal VPS13A (H-102). ( B ) Full length VPS13A-GFP ( B ) or VPS13A ∆FFAT -GFP ( B’ ) were expressed in HEK293T cells and mitochondria were marked using an antibody against TOMM20. The yellow signal in the overlay represents sites of close association between mitochondria and the VPS13A-GFP. Cells were co-transfected with BFP-Sec61 (not shown). ( C ) HEK293T cells were transfected with VPS13A-GFP or VPS13A ∆FFAT -GFP and stained for TOMM20. The fraction of the GFP signal overlapping the TOMM20 signal was quantified with ImageJ using the JACoP plugin. ( D ) HEK293T cells were transfected with VPS13A-GFP or VPS13A ∆FFAT -GFP and mCherry-VAP-A. The fraction of the GFP signal overlapping the mCherry signal was quantified with ImageJ using the JACoP plugin. Error bars ( C, D ), mean ±s.e.m (n = 3), two-tailed unpaired Student’s t-test was used (**p≤0.01). ( E ) Full length VPS13A-GFP, VPS13A ∆FFAT -GFP or peGFP-C1 (as a control) were expressed in HEK293T cells and immunoprecipitated using a GFP-trap assay. Samples from Figure 4F were co-analysed for the presence of VAP-B. Scale bar = 10 µm (B/B’).

    Journal: eLife

    Article Title: Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility

    doi: 10.7554/eLife.43561

    Figure Lengend Snippet: VPS13A interacts with VAP-A. ( A ) GST-fusion proteins of VPS13A fragments expressed in E.Coli were enriched on Sepharose beads and incubated with equal amounts of HeLa cell lysate. GST alone was used as a control. Samples were immunoblotted against VAP-A, GST and N-terminal VPS13A (H-102). ( B ) Full length VPS13A-GFP ( B ) or VPS13A ∆FFAT -GFP ( B’ ) were expressed in HEK293T cells and mitochondria were marked using an antibody against TOMM20. The yellow signal in the overlay represents sites of close association between mitochondria and the VPS13A-GFP. Cells were co-transfected with BFP-Sec61 (not shown). ( C ) HEK293T cells were transfected with VPS13A-GFP or VPS13A ∆FFAT -GFP and stained for TOMM20. The fraction of the GFP signal overlapping the TOMM20 signal was quantified with ImageJ using the JACoP plugin. ( D ) HEK293T cells were transfected with VPS13A-GFP or VPS13A ∆FFAT -GFP and mCherry-VAP-A. The fraction of the GFP signal overlapping the mCherry signal was quantified with ImageJ using the JACoP plugin. Error bars ( C, D ), mean ±s.e.m (n = 3), two-tailed unpaired Student’s t-test was used (**p≤0.01). ( E ) Full length VPS13A-GFP, VPS13A ∆FFAT -GFP or peGFP-C1 (as a control) were expressed in HEK293T cells and immunoprecipitated using a GFP-trap assay. Samples from Figure 4F were co-analysed for the presence of VAP-B. Scale bar = 10 µm (B/B’).

    Article Snippet: To obtain GFP-VAP-A, VAP-A was amplified by PCR from the His-VAP-A plasmid and inserted into pEGFP-C1 (Clontech) via EcoRI and BamHI restriction sites.

    Techniques: Incubation, Transfection, Staining, Two Tailed Test, Immunoprecipitation, TRAP Assay

    Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) mCerulean3-α-tubulin-6 (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.

    Journal: PLoS ONE

    Article Title: An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

    doi: 10.1371/journal.pone.0017896

    Figure Lengend Snippet: Fluorescence imaging of mCerulean3 fusion vectors. Images were recorded in widefield or laser scanning confocal fluorescence microscopy. (A–M) Fusions to the N-terminus of mCerulean3; for each fusion protein the linker amino acid (aa) length is indicated after the name of the targeted organelle or fusion protein. The origin of the targeting cDNA is indicated in parenthesis. (A) mCerulean3-Cx43-7 (rat); (B) mCerulean3-EB3-7 (human microtubule-associated protein; RP/EB family); (C) mCerulean3-Golgi-7 (N-terminal 81 aa of human β-1,4-galactosyltransferase); (D) mCerulean3-α-actinin (human); (E) mCerulean3-PMP-10 (human peroxisomal membrane protein 2); (F) mCerulean3-c-src-7 (chicken c-src tyrosine kinase); (G) mCerulean3-mitochondria-7 (human cytochrome C oxidase subunit VIII); (H) mCerulean3-zyxin-7 (human); (I) mCerulean3-vimentin-7 (human); (J) mCerulean3-lifeact-7 (N-terminal 17 aa from S. cerevisiae Abp 140); (K) mCerulean3-VE-Cadherin-10 (human vascular epithelial cadherin); (L) mCerulean3-fascin-10 (human fascin); (M) mCerulean3-lysosomes-20 (human lysosomal membrane glycoprotein 1; LAMP-1). (N–Y) Fusions to the C-terminus of mCerulean3. (N) mCerulean3-lamin B1-10 (human); (O) mCerulean-MAP4-10 (mouse microtubule associated protein 4, nucleotides 1918–3135); (P) mCerulean3-lc-myosin-10 (mouse myosin light chain 9); (Q) mCerulean3-CDC42-10 (human cell division cycle 42); (R) mCerulean3-α-tubulin-6 (human); (S) mCerulean3-PCNA-19 (human proliferating cell nuclear antigen); (T) mCerulean3-profilin-10 (mouse profilin); (U) mCerulean3-clathrin light chain-15 (human); (V) mCerulean3-CAF1-10 (mouse chromatin assembly factor 1); (W) mCerulean3-fibrillarin-7 (human fibrillarin); (X) mCerulean3-β-actin-7 (human); (Y) mCerulean-Rab5a-7 (human GTPase Rab5a). (Z1–Z5) mCerulean3-H2B-6 (human) illustrating the various phases of mitosis. (Z1) interphase; (Z2) prophase; (Z3) metaphase; (Z4) anaphase; (Z5) early telophase. Scale bars indicate 10 µm.

    Article Snippet: To prepare mCerulean3 C-terminal fusions, the following digests were performed: human β-actin, NheI and BglII (Clontech); human α-tubulin, NheI and BglII (Clontech); human light chain clathrin, NheI and BglII (George Patterson, NIH); human lamin B1, NheI and BglII (George Patterson, NIH); mouse MAP4, NheI and BglII (mouse microtubule associated protein 4, nucleotides 1918–3135, Richard Cyr, Penn State University); mouse light chain 9 myosin, NheI and BglII (Patricia Wadsworth, University of Massachusetts); human CDC-42, NheI and BglII (OriGene); PCNA, AgeI and BspEI (David Gilbert, FSU); mouse CAF-1, NheI and BglII (Akash Gunjan, FSU); human fibrillarin, AgeI and BspEI (Dimitry Chudakov, Russian Academy of Sciences); human GTPase Rab5a, NheI and BglII (Vicky Allen, University of Manchester).

    Techniques: Fluorescence, Imaging, Microscopy