bsa Promega Search Results


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  • 95
    New England Biolabs bsa hi
    Bsa Hi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa hi/product/New England Biolabs
    Average 95 stars, based on 57 article reviews
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    bsa hi - by Bioz Stars, 2020-02
    95/100 stars
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    95
    Millipore bovine serum albumin bsa
    Effect of <t>BSA</t> type on the TaqMan-based PCR yield. Acetylated BSA from Ambion and Promega, non-acetylated BSA from <t>Sigma</t> were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).
    Bovine Serum Albumin Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 24233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 24233 article reviews
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    79
    Promega biotin streptavidin promega enrichment procedure
    Effect of <t>BSA</t> type on the TaqMan-based PCR yield. Acetylated BSA from Ambion and Promega, non-acetylated BSA from <t>Sigma</t> were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).
    Biotin Streptavidin Promega Enrichment Procedure, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 10 article reviews
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    90
    Thermo Fisher bsa
    Effect of <t>BSA</t> type on the TaqMan-based PCR yield. Acetylated BSA from <t>Ambion</t> and Promega, non-acetylated BSA from Sigma were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).
    Bsa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 25730 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 25730 article reviews
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    bsa - by Bioz Stars, 2020-02
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    95
    Promega blot qualified bsa
    Effect of <t>BSA</t> type on the TaqMan-based PCR yield. Acetylated BSA from <t>Ambion</t> and Promega, non-acetylated BSA from Sigma were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).
    Blot Qualified Bsa, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Promega n acetylated bsa
    MASP-2 is essential for C3 deposition on S pneumoniae . Serial dilutions of sera were incubated in microtiter plates coated with S. pneumoniae D39 or N-acetylated <t>BSA</t> (as a control), and C3 deposition determined by ELISA. A. Shows the raw data from one experiment with WT and MASP-2 deficient murine serum (means ±SEM). In B , the experiment was extended to include murine sera deficient in other complement components. Results are duplicates (±SD) and are normalised to the C3 deposition observed in the WT control. C. Time course of C3 activation on S. pneumoniae . 1∶40 diluted murine sera were incubated in microtiter plates coated with S. pneumoniae for the times indicated then C3 deposition assayed. Results are means of duplicates and are representative of three independent experiments. D. FACS analysis of C3 deposition on S. pneumoniae opsonised with MASP-2 −/− serum (black line), WT serum (red) and C4 −/− serum (green). The blue trace shows non-opsonised bacteria. E. Results from 3 independent FACS analyses of C3 deposition (mean fluorescent intensity ±SEM). F. Inhibition of MASP-2 activity with mAb AbD04211 abolished C3b deposition on S. pneumoniae opsonised with C4 deficient serum. (means of triplicates ±SEM; p value from Student's t-test). G. In human serum, <t>MBL</t> deficiency had no effect on C3 deposition, while C4 deficiency had no significant effect on the EC 50 , but reduces absolute C3b deposition by about 50% (means ±SEM; n = 3 for NHS and MBL −/− serum, 1 for C4 deficient serum). H. Correlation between L-ficolin serum concentration and C3b deposition on S. pneumoniae immobilised on microtiter plates for 47 samples of NHS (solid line shows Fisher transformation of Pearson's correlation coefficient; dashed lines, 95% CI thereof).
    N Acetylated Bsa, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Promega blot quality bsa
    MASP-2 is essential for C3 deposition on S pneumoniae . Serial dilutions of sera were incubated in microtiter plates coated with S. pneumoniae D39 or N-acetylated <t>BSA</t> (as a control), and C3 deposition determined by ELISA. A. Shows the raw data from one experiment with WT and MASP-2 deficient murine serum (means ±SEM). In B , the experiment was extended to include murine sera deficient in other complement components. Results are duplicates (±SD) and are normalised to the C3 deposition observed in the WT control. C. Time course of C3 activation on S. pneumoniae . 1∶40 diluted murine sera were incubated in microtiter plates coated with S. pneumoniae for the times indicated then C3 deposition assayed. Results are means of duplicates and are representative of three independent experiments. D. FACS analysis of C3 deposition on S. pneumoniae opsonised with MASP-2 −/− serum (black line), WT serum (red) and C4 −/− serum (green). The blue trace shows non-opsonised bacteria. E. Results from 3 independent FACS analyses of C3 deposition (mean fluorescent intensity ±SEM). F. Inhibition of MASP-2 activity with mAb AbD04211 abolished C3b deposition on S. pneumoniae opsonised with C4 deficient serum. (means of triplicates ±SEM; p value from Student's t-test). G. In human serum, <t>MBL</t> deficiency had no effect on C3 deposition, while C4 deficiency had no significant effect on the EC 50 , but reduces absolute C3b deposition by about 50% (means ±SEM; n = 3 for NHS and MBL −/− serum, 1 for C4 deficient serum). H. Correlation between L-ficolin serum concentration and C3b deposition on S. pneumoniae immobilised on microtiter plates for 47 samples of NHS (solid line shows Fisher transformation of Pearson's correlation coefficient; dashed lines, 95% CI thereof).
    Blot Quality Bsa, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 6 article reviews
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    76
    Promega 1x acetylated bsa
    MASP-2 is essential for C3 deposition on S pneumoniae . Serial dilutions of sera were incubated in microtiter plates coated with S. pneumoniae D39 or N-acetylated <t>BSA</t> (as a control), and C3 deposition determined by ELISA. A. Shows the raw data from one experiment with WT and MASP-2 deficient murine serum (means ±SEM). In B , the experiment was extended to include murine sera deficient in other complement components. Results are duplicates (±SD) and are normalised to the C3 deposition observed in the WT control. C. Time course of C3 activation on S. pneumoniae . 1∶40 diluted murine sera were incubated in microtiter plates coated with S. pneumoniae for the times indicated then C3 deposition assayed. Results are means of duplicates and are representative of three independent experiments. D. FACS analysis of C3 deposition on S. pneumoniae opsonised with MASP-2 −/− serum (black line), WT serum (red) and C4 −/− serum (green). The blue trace shows non-opsonised bacteria. E. Results from 3 independent FACS analyses of C3 deposition (mean fluorescent intensity ±SEM). F. Inhibition of MASP-2 activity with mAb AbD04211 abolished C3b deposition on S. pneumoniae opsonised with C4 deficient serum. (means of triplicates ±SEM; p value from Student's t-test). G. In human serum, <t>MBL</t> deficiency had no effect on C3 deposition, while C4 deficiency had no significant effect on the EC 50 , but reduces absolute C3b deposition by about 50% (means ±SEM; n = 3 for NHS and MBL −/− serum, 1 for C4 deficient serum). H. Correlation between L-ficolin serum concentration and C3b deposition on S. pneumoniae immobilised on microtiter plates for 47 samples of NHS (solid line shows Fisher transformation of Pearson's correlation coefficient; dashed lines, 95% CI thereof).
    1x Acetylated Bsa, supplied by Promega, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega bovine serum albumin bsa
    Schematic representation of ESAT-6 detection by the <t>biotin-streptavidin</t> interaction. Complete schematics are displayed. ESAT-6 protein was coated onto the plate followed by anti-ESAT-6 and <t>biotinylated</t> anti-mouse-IgG. Streptavidin-HRP with and without free biotin then was added.
    Bovine Serum Albumin Bsa, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Promega bovine serum albumen
    Schematic representation of ESAT-6 detection by the <t>biotin-streptavidin</t> interaction. Complete schematics are displayed. ESAT-6 protein was coated onto the plate followed by anti-ESAT-6 and <t>biotinylated</t> anti-mouse-IgG. Streptavidin-HRP with and without free biotin then was added.
    Bovine Serum Albumen, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Boehringer Mannheim fatty acid free bsa
    Schematic representation of ESAT-6 detection by the <t>biotin-streptavidin</t> interaction. Complete schematics are displayed. ESAT-6 protein was coated onto the plate followed by anti-ESAT-6 and <t>biotinylated</t> anti-mouse-IgG. Streptavidin-HRP with and without free biotin then was added.
    Fatty Acid Free Bsa, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 91/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Promega mgml 1 bovine serum albumen
    Schematic representation of ESAT-6 detection by the <t>biotin-streptavidin</t> interaction. Complete schematics are displayed. ESAT-6 protein was coated onto the plate followed by anti-ESAT-6 and <t>biotinylated</t> anti-mouse-IgG. Streptavidin-HRP with and without free biotin then was added.
    Mgml 1 Bovine Serum Albumen, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega viviren
    In vivo heat-shock efficiency. ( A ) Subcutaneous xenografts were generated by the injection of miRLuc or miRneg cell lines in mice hind legs. Representative image of LucR signal detection for miRLuc and miRneg tumor following intra-peritoneal injection of <t>ViviRen.</t> ( B ) Relative quantification (RQ) of miRLuc and miRneg from miLuc and miRneg tumors respectively determined by qRT-PCR at different time after hyperthermia (n = 3) (Student’s t-test, *p
    Viviren, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega dynabeads
    In vivo heat-shock efficiency. ( A ) Subcutaneous xenografts were generated by the injection of miRLuc or miRneg cell lines in mice hind legs. Representative image of LucR signal detection for miRLuc and miRneg tumor following intra-peritoneal injection of <t>ViviRen.</t> ( B ) Relative quantification (RQ) of miRLuc and miRneg from miLuc and miRneg tumors respectively determined by qRT-PCR at different time after hyperthermia (n = 3) (Student’s t-test, *p
    Dynabeads, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Promega antibody rabbit anti human osteopontin
    ( A ) Immunofluorescence of <t>osteopontin</t> (green) in MONO and CO capsules cultured in EG and EDAG media after 21 days of culture. Cells nuclei were counterstained with DAPI (blue). To visualize the encapsulated ECs, CD31 (red) was identified by immunofluorescence staining in histological sections of CO capsules cultured for 21 days (scale bar: 50 μm). ( B ) Quantification of BMP-2 and VEGF release by ELISA. ( C ) Relative expression of osteogenic ( BMP-2, RUNX2, and BSP ) and angiogenic ( VEGF, CD31 , and vWF ) markers up to 21 days. All results were significantly different unless marked with ns (p > 0.05).
    Antibody Rabbit Anti Human Osteopontin, supplied by Promega, used in various techniques. Bioz Stars score: 83/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega dnase i
    ( A ) Immunofluorescence of <t>osteopontin</t> (green) in MONO and CO capsules cultured in EG and EDAG media after 21 days of culture. Cells nuclei were counterstained with DAPI (blue). To visualize the encapsulated ECs, CD31 (red) was identified by immunofluorescence staining in histological sections of CO capsules cultured for 21 days (scale bar: 50 μm). ( B ) Quantification of BMP-2 and VEGF release by ELISA. ( C ) Relative expression of osteogenic ( BMP-2, RUNX2, and BSP ) and angiogenic ( VEGF, CD31 , and vWF ) markers up to 21 days. All results were significantly different unless marked with ns (p > 0.05).
    Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 10850 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Promega gst pulldown buffer
    ( A ) Immunofluorescence of <t>osteopontin</t> (green) in MONO and CO capsules cultured in EG and EDAG media after 21 days of culture. Cells nuclei were counterstained with DAPI (blue). To visualize the encapsulated ECs, CD31 (red) was identified by immunofluorescence staining in histological sections of CO capsules cultured for 21 days (scale bar: 50 μm). ( B ) Quantification of BMP-2 and VEGF release by ELISA. ( C ) Relative expression of osteogenic ( BMP-2, RUNX2, and BSP ) and angiogenic ( VEGF, CD31 , and vWF ) markers up to 21 days. All results were significantly different unless marked with ns (p > 0.05).
    Gst Pulldown Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega buffer
    ( A ) Immunofluorescence of <t>osteopontin</t> (green) in MONO and CO capsules cultured in EG and EDAG media after 21 days of culture. Cells nuclei were counterstained with DAPI (blue). To visualize the encapsulated ECs, CD31 (red) was identified by immunofluorescence staining in histological sections of CO capsules cultured for 21 days (scale bar: 50 μm). ( B ) Quantification of BMP-2 and VEGF release by ELISA. ( C ) Relative expression of osteogenic ( BMP-2, RUNX2, and BSP ) and angiogenic ( VEGF, CD31 , and vWF ) markers up to 21 days. All results were significantly different unless marked with ns (p > 0.05).
    Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1613 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega horseradish peroxidase
    ( A ) Immunofluorescence of <t>osteopontin</t> (green) in MONO and CO capsules cultured in EG and EDAG media after 21 days of culture. Cells nuclei were counterstained with DAPI (blue). To visualize the encapsulated ECs, CD31 (red) was identified by immunofluorescence staining in histological sections of CO capsules cultured for 21 days (scale bar: 50 μm). ( B ) Quantification of BMP-2 and VEGF release by ELISA. ( C ) Relative expression of osteogenic ( BMP-2, RUNX2, and BSP ) and angiogenic ( VEGF, CD31 , and vWF ) markers up to 21 days. All results were significantly different unless marked with ns (p > 0.05).
    Horseradish Peroxidase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Promega alkaline phosphatase conjugated secondary anti mouse antibody
    ( A ) Immunofluorescence of <t>osteopontin</t> (green) in MONO and CO capsules cultured in EG and EDAG media after 21 days of culture. Cells nuclei were counterstained with DAPI (blue). To visualize the encapsulated ECs, CD31 (red) was identified by immunofluorescence staining in histological sections of CO capsules cultured for 21 days (scale bar: 50 μm). ( B ) Quantification of BMP-2 and VEGF release by ELISA. ( C ) Relative expression of osteogenic ( BMP-2, RUNX2, and BSP ) and angiogenic ( VEGF, CD31 , and vWF ) markers up to 21 days. All results were significantly different unless marked with ns (p > 0.05).
    Alkaline Phosphatase Conjugated Secondary Anti Mouse Antibody, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega lysis solution
    ( A ) Immunofluorescence of <t>osteopontin</t> (green) in MONO and CO capsules cultured in EG and EDAG media after 21 days of culture. Cells nuclei were counterstained with DAPI (blue). To visualize the encapsulated ECs, CD31 (red) was identified by immunofluorescence staining in histological sections of CO capsules cultured for 21 days (scale bar: 50 μm). ( B ) Quantification of BMP-2 and VEGF release by ELISA. ( C ) Relative expression of osteogenic ( BMP-2, RUNX2, and BSP ) and angiogenic ( VEGF, CD31 , and vWF ) markers up to 21 days. All results were significantly different unless marked with ns (p > 0.05).
    Lysis Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega bovine pka
    Elevated <t>PKA</t> activity results in WC-independent frq transcription. (A) Circadian conidiation rhythm of the wild-type and <t>pkac-1</t> KO strains and the pkac-1 KO , qa-Myc-His-PKAC-1 transformants in the race tube in the presence of QA (10 −2 M). (B) Race
    Bovine Pka, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega rnasin
    Elevated <t>PKA</t> activity results in WC-independent frq transcription. (A) Circadian conidiation rhythm of the wild-type and <t>pkac-1</t> KO strains and the pkac-1 KO , qa-Myc-His-PKAC-1 transformants in the race tube in the presence of QA (10 −2 M). (B) Race
    Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 17927 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Promega type i bovine pka
    Elevated <t>PKA</t> activity results in WC-independent frq transcription. (A) Circadian conidiation rhythm of the wild-type and <t>pkac-1</t> KO strains and the pkac-1 KO , qa-Myc-His-PKAC-1 transformants in the race tube in the presence of QA (10 −2 M). (B) Race
    Type I Bovine Pka, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega glo lysis buffer
    Elevated <t>PKA</t> activity results in WC-independent frq transcription. (A) Circadian conidiation rhythm of the wild-type and <t>pkac-1</t> KO strains and the pkac-1 KO , qa-Myc-His-PKAC-1 transformants in the race tube in the presence of QA (10 −2 M). (B) Race
    Glo Lysis Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Promega bovine heart rii
    Elevated <t>PKA</t> activity results in WC-independent frq transcription. (A) Circadian conidiation rhythm of the wild-type and <t>pkac-1</t> KO strains and the pkac-1 KO , qa-Myc-His-PKAC-1 transformants in the race tube in the presence of QA (10 −2 M). (B) Race
    Bovine Heart Rii, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega 1x bovine serum albumin
    Elevated <t>PKA</t> activity results in WC-independent frq transcription. (A) Circadian conidiation rhythm of the wild-type and <t>pkac-1</t> KO strains and the pkac-1 KO , qa-Myc-His-PKAC-1 transformants in the race tube in the presence of QA (10 −2 M). (B) Race
    1x Bovine Serum Albumin, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Promega bovine fetuin
    Phosphatase and glycosidase treatments do not affect altered proteasome activity profile. (A) Root extracts of 250 mM treated samples, labeled by MVB072 were treated with alkaline phosphatase at different conditions. (B) Dephosphorylation of MAP kinase, used as a positive control, detected by an anti-phosphoMAPK antibody. (C) Root extracts of 250 mM treated-samples, labeled by MVB072 were treated with and without PNGase F or <t>deglycosylation</t> mix. (D) Enzymatic deglycosylation of Bovine <t>Fetuin</t> was used as a positive control.
    Bovine Fetuin, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Promega bovine tafi
    Phosphatase and glycosidase treatments do not affect altered proteasome activity profile. (A) Root extracts of 250 mM treated samples, labeled by MVB072 were treated with alkaline phosphatase at different conditions. (B) Dephosphorylation of MAP kinase, used as a positive control, detected by an anti-phosphoMAPK antibody. (C) Root extracts of 250 mM treated-samples, labeled by MVB072 were treated with and without PNGase F or <t>deglycosylation</t> mix. (D) Enzymatic deglycosylation of Bovine <t>Fetuin</t> was used as a positive control.
    Bovine Tafi, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of BSA type on the TaqMan-based PCR yield. Acetylated BSA from Ambion and Promega, non-acetylated BSA from Sigma were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).

    Journal: Biomicrofluidics

    Article Title: Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices

    doi: 10.1063/1.4983692

    Figure Lengend Snippet: Effect of BSA type on the TaqMan-based PCR yield. Acetylated BSA from Ambion and Promega, non-acetylated BSA from Sigma were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).

    Article Snippet: Based on optimized conditions, the PCR mixture contained 10 mM Tris-HCl (pH 9.0), 50 mM KCl, and 0.1% Triton X-100, 0.2 mM each of dATP, dCTP, dTTP and dGTP (PCR Nucleotide Mix, Promega, Madison, P/N C1141), 3 mM MgCl2 (Invitrogen by Life Technologies, Carlsbad, P/N 10966), 0.3 μ M each of forward and reverse gene specific primer (1st BASE, Singapore), 0.1 U/ μ l of hot-start Platinum Taq DNA polymerase (Invitrogen by Life Technologies, Carlsbad, P/N 10966), various concentration and types of bovine serum albumin (BSA) (Sigma Aldrich, P/N B 8667; Promega P/N R3961), 1× SYBR Green I (Cambrex Biosciences, Maine, USA), and 0.1 ng/ μ l (3 × 107 copies) of plasmid pGEM-3Z DNA template.

    Techniques: Polymerase Chain Reaction, Concentration Assay

    Effect of BSA type on the TaqMan-based PCR yield. Acetylated BSA from Ambion and Promega, non-acetylated BSA from Sigma were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).

    Journal: Biomicrofluidics

    Article Title: Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices

    doi: 10.1063/1.4983692

    Figure Lengend Snippet: Effect of BSA type on the TaqMan-based PCR yield. Acetylated BSA from Ambion and Promega, non-acetylated BSA from Sigma were tested at 1 μ g/ μ l concentration in polypropylene tubes. Diamond illustrates a sample mean (center line) and 95% confidence interval (height).

    Article Snippet: Note that acetylated BSA from Ambion shows a more diffused band compared to acetylated BSA from Promega.

    Techniques: Polymerase Chain Reaction, Concentration Assay

    MASP-2 is essential for C3 deposition on S pneumoniae . Serial dilutions of sera were incubated in microtiter plates coated with S. pneumoniae D39 or N-acetylated BSA (as a control), and C3 deposition determined by ELISA. A. Shows the raw data from one experiment with WT and MASP-2 deficient murine serum (means ±SEM). In B , the experiment was extended to include murine sera deficient in other complement components. Results are duplicates (±SD) and are normalised to the C3 deposition observed in the WT control. C. Time course of C3 activation on S. pneumoniae . 1∶40 diluted murine sera were incubated in microtiter plates coated with S. pneumoniae for the times indicated then C3 deposition assayed. Results are means of duplicates and are representative of three independent experiments. D. FACS analysis of C3 deposition on S. pneumoniae opsonised with MASP-2 −/− serum (black line), WT serum (red) and C4 −/− serum (green). The blue trace shows non-opsonised bacteria. E. Results from 3 independent FACS analyses of C3 deposition (mean fluorescent intensity ±SEM). F. Inhibition of MASP-2 activity with mAb AbD04211 abolished C3b deposition on S. pneumoniae opsonised with C4 deficient serum. (means of triplicates ±SEM; p value from Student's t-test). G. In human serum, MBL deficiency had no effect on C3 deposition, while C4 deficiency had no significant effect on the EC 50 , but reduces absolute C3b deposition by about 50% (means ±SEM; n = 3 for NHS and MBL −/− serum, 1 for C4 deficient serum). H. Correlation between L-ficolin serum concentration and C3b deposition on S. pneumoniae immobilised on microtiter plates for 47 samples of NHS (solid line shows Fisher transformation of Pearson's correlation coefficient; dashed lines, 95% CI thereof).

    Journal: PLoS Pathogens

    Article Title: The Lectin Pathway of Complement Activation Is a Critical Component of the Innate Immune Response to Pneumococcal Infection

    doi: 10.1371/journal.ppat.1002793

    Figure Lengend Snippet: MASP-2 is essential for C3 deposition on S pneumoniae . Serial dilutions of sera were incubated in microtiter plates coated with S. pneumoniae D39 or N-acetylated BSA (as a control), and C3 deposition determined by ELISA. A. Shows the raw data from one experiment with WT and MASP-2 deficient murine serum (means ±SEM). In B , the experiment was extended to include murine sera deficient in other complement components. Results are duplicates (±SD) and are normalised to the C3 deposition observed in the WT control. C. Time course of C3 activation on S. pneumoniae . 1∶40 diluted murine sera were incubated in microtiter plates coated with S. pneumoniae for the times indicated then C3 deposition assayed. Results are means of duplicates and are representative of three independent experiments. D. FACS analysis of C3 deposition on S. pneumoniae opsonised with MASP-2 −/− serum (black line), WT serum (red) and C4 −/− serum (green). The blue trace shows non-opsonised bacteria. E. Results from 3 independent FACS analyses of C3 deposition (mean fluorescent intensity ±SEM). F. Inhibition of MASP-2 activity with mAb AbD04211 abolished C3b deposition on S. pneumoniae opsonised with C4 deficient serum. (means of triplicates ±SEM; p value from Student's t-test). G. In human serum, MBL deficiency had no effect on C3 deposition, while C4 deficiency had no significant effect on the EC 50 , but reduces absolute C3b deposition by about 50% (means ±SEM; n = 3 for NHS and MBL −/− serum, 1 for C4 deficient serum). H. Correlation between L-ficolin serum concentration and C3b deposition on S. pneumoniae immobilised on microtiter plates for 47 samples of NHS (solid line shows Fisher transformation of Pearson's correlation coefficient; dashed lines, 95% CI thereof).

    Article Snippet: Solid phase binding assays Nunc Maxisorb microtiter plates were coated with 100 µl of the following reagents: 10 µg/ml mannan (a control for MBL binding), 10 µg/ml zymosan (a control for CL-11 binding), 10 µg/ml N-acetylated BSA (Promega; a control for ficolin A binding), 5 µg/ml of the FCN2-specific mAb GN4, 10 µg/ml PSA, or formalin-fixed S. pneumoniae D39 (OD550 nm = 0.6) in coating buffer (15 mM Na2 CO3 , 35 mM NaHCO3 , pH 9.6).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Activation Assay, FACS, Inhibition, Activity Assay, Concentration Assay, Transformation Assay

    Schematic representation of ESAT-6 detection by the biotin-streptavidin interaction. Complete schematics are displayed. ESAT-6 protein was coated onto the plate followed by anti-ESAT-6 and biotinylated anti-mouse-IgG. Streptavidin-HRP with and without free biotin then was added.

    Journal: PLoS ONE

    Article Title: Biotin-Streptavidin Competition Mediates Sensitive Detection of Biomolecules in Enzyme Linked Immunosorbent Assay

    doi: 10.1371/journal.pone.0151153

    Figure Lengend Snippet: Schematic representation of ESAT-6 detection by the biotin-streptavidin interaction. Complete schematics are displayed. ESAT-6 protein was coated onto the plate followed by anti-ESAT-6 and biotinylated anti-mouse-IgG. Streptavidin-HRP with and without free biotin then was added.

    Article Snippet: Anti-ESAT-6 was obtained from Santa Cruz Biotechnology (USA), biotinylated anti-mouse-IgG was from Invitrogen (USA), streptavidin-HRP was procured from Thermo-Scientific (Japan), bovine serum albumin (BSA) was from Promega (USA), and.

    Techniques:

    In vivo heat-shock efficiency. ( A ) Subcutaneous xenografts were generated by the injection of miRLuc or miRneg cell lines in mice hind legs. Representative image of LucR signal detection for miRLuc and miRneg tumor following intra-peritoneal injection of ViviRen. ( B ) Relative quantification (RQ) of miRLuc and miRneg from miLuc and miRneg tumors respectively determined by qRT-PCR at different time after hyperthermia (n = 3) (Student’s t-test, *p

    Journal: Scientific Reports

    Article Title: Imaging of conditional gene silencing in vivo using a bioluminescence-based method with thermo-inducible microRNAs

    doi: 10.1038/s41598-018-22932-3

    Figure Lengend Snippet: In vivo heat-shock efficiency. ( A ) Subcutaneous xenografts were generated by the injection of miRLuc or miRneg cell lines in mice hind legs. Representative image of LucR signal detection for miRLuc and miRneg tumor following intra-peritoneal injection of ViviRen. ( B ) Relative quantification (RQ) of miRLuc and miRneg from miLuc and miRneg tumors respectively determined by qRT-PCR at different time after hyperthermia (n = 3) (Student’s t-test, *p

    Article Snippet: For LucR signal detection, mice received an intra-peritoneal injection of ViviRen (Promega, 50.8 µg in 100 µL PBS-BSA 0.1%) and were sedated 17 min later.

    Techniques: In Vivo, Generated, Injection, Mouse Assay, Quantitative RT-PCR

    ( A ) Immunofluorescence of osteopontin (green) in MONO and CO capsules cultured in EG and EDAG media after 21 days of culture. Cells nuclei were counterstained with DAPI (blue). To visualize the encapsulated ECs, CD31 (red) was identified by immunofluorescence staining in histological sections of CO capsules cultured for 21 days (scale bar: 50 μm). ( B ) Quantification of BMP-2 and VEGF release by ELISA. ( C ) Relative expression of osteogenic ( BMP-2, RUNX2, and BSP ) and angiogenic ( VEGF, CD31 , and vWF ) markers up to 21 days. All results were significantly different unless marked with ns (p > 0.05).

    Journal: Scientific Reports

    Article Title: Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation

    doi: 10.1038/srep21883

    Figure Lengend Snippet: ( A ) Immunofluorescence of osteopontin (green) in MONO and CO capsules cultured in EG and EDAG media after 21 days of culture. Cells nuclei were counterstained with DAPI (blue). To visualize the encapsulated ECs, CD31 (red) was identified by immunofluorescence staining in histological sections of CO capsules cultured for 21 days (scale bar: 50 μm). ( B ) Quantification of BMP-2 and VEGF release by ELISA. ( C ) Relative expression of osteogenic ( BMP-2, RUNX2, and BSP ) and angiogenic ( VEGF, CD31 , and vWF ) markers up to 21 days. All results were significantly different unless marked with ns (p > 0.05).

    Article Snippet: Samples were immersed in BSA (3% w/v) for 30 min at RT to block non-specific binding and then incubated overnight at 4 °C with the primary antibody rabbit anti-human osteopontin (Promega, 1:100 in 1% BSA).

    Techniques: Immunofluorescence, Cell Culture, Staining, Enzyme-linked Immunosorbent Assay, Expressing

    Elevated PKA activity results in WC-independent frq transcription. (A) Circadian conidiation rhythm of the wild-type and pkac-1 KO strains and the pkac-1 KO , qa-Myc-His-PKAC-1 transformants in the race tube in the presence of QA (10 −2 M). (B) Race

    Journal: Molecular and Cellular Biology

    Article Title: Role for Protein Kinase A in the Neurospora Circadian Clock by Regulating White Collar-Independent frequency Transcription through Phosphorylation of RCM-1

    doi: 10.1128/MCB.00709-14

    Figure Lengend Snippet: Elevated PKA activity results in WC-independent frq transcription. (A) Circadian conidiation rhythm of the wild-type and pkac-1 KO strains and the pkac-1 KO , qa-Myc-His-PKAC-1 transformants in the race tube in the presence of QA (10 −2 M). (B) Race

    Article Snippet: For the in vitro phosphorylation assay, GST/RCM-1 fusion protein were purified from Escherichia coli BL21 cells and incubated with bovine PKA (Promega) or partially purified Myc/His/PKAC-1 from Neurospora .

    Techniques: Activity Assay

    Phosphatase and glycosidase treatments do not affect altered proteasome activity profile. (A) Root extracts of 250 mM treated samples, labeled by MVB072 were treated with alkaline phosphatase at different conditions. (B) Dephosphorylation of MAP kinase, used as a positive control, detected by an anti-phosphoMAPK antibody. (C) Root extracts of 250 mM treated-samples, labeled by MVB072 were treated with and without PNGase F or deglycosylation mix. (D) Enzymatic deglycosylation of Bovine Fetuin was used as a positive control.

    Journal: Frontiers in Plant Science

    Article Title: Proteasome Activity Profiling Uncovers Alteration of Catalytic β2 and β5 Subunits of the Stress-Induced Proteasome during Salinity Stress in Tomato Roots

    doi: 10.3389/fpls.2017.00107

    Figure Lengend Snippet: Phosphatase and glycosidase treatments do not affect altered proteasome activity profile. (A) Root extracts of 250 mM treated samples, labeled by MVB072 were treated with alkaline phosphatase at different conditions. (B) Dephosphorylation of MAP kinase, used as a positive control, detected by an anti-phosphoMAPK antibody. (C) Root extracts of 250 mM treated-samples, labeled by MVB072 were treated with and without PNGase F or deglycosylation mix. (D) Enzymatic deglycosylation of Bovine Fetuin was used as a positive control.

    Article Snippet: Protein Deglycosylation of Labeled Proteins Eighteen microliter of MVB072-labelled sample and Bovine Fetuin (Promega) were treated with 2 μl of 10X denaturing solution (Promega) and heated at 95°C for 10 min.

    Techniques: Activity Assay, Labeling, De-Phosphorylation Assay, Positive Control