bs-5125R Search Results


fzd7  (Bioss)
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Bioss fzd7
DL -methionine and DL -methionyl- DL -methionine increase abundance of <t>FZD7</t> and activate Wnt/β-catenin signaling pathway in pigeon jejunal tissue. (A) The expression of Wnt/β-catenin signaling pathway-associated proteins in the jejunum of pigeons with DL -Met or DL -Met-Met. (B) Statistical analysis of the expression of target proteins based on the images shown in (A). (C) Representative images of IF staining with FZD7 antibodies of the pigeon jejunum (100 ×). Scale bar = 100 μm. (D) Statistical analysis of the expression of FZD7 based on the images shown in (C). (E, F) Binding mode of DL - Met to FZD7 (E) and DL -Met - Met to FZD7 (F). The protein is shown in cartoon form. Surrounding residues are represented in stick form. The dashed lines indicate the formation of hydrogen bonds. The numbers beside the dash show the distance (Å) of hydrogen bonds. “a-c” indicates significant differences ( P < 0.05). The values are mean ±S.E.M. with n = 3.
Fzd7, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DL -methionine and DL -methionyl- DL -methionine increase abundance of FZD7 and activate Wnt/β-catenin signaling pathway in pigeon jejunal tissue. (A) The expression of Wnt/β-catenin signaling pathway-associated proteins in the jejunum of pigeons with DL -Met or DL -Met-Met. (B) Statistical analysis of the expression of target proteins based on the images shown in (A). (C) Representative images of IF staining with FZD7 antibodies of the pigeon jejunum (100 ×). Scale bar = 100 μm. (D) Statistical analysis of the expression of FZD7 based on the images shown in (C). (E, F) Binding mode of DL - Met to FZD7 (E) and DL -Met - Met to FZD7 (F). The protein is shown in cartoon form. Surrounding residues are represented in stick form. The dashed lines indicate the formation of hydrogen bonds. The numbers beside the dash show the distance (Å) of hydrogen bonds. “a-c” indicates significant differences ( P < 0.05). The values are mean ±S.E.M. with n = 3.

Journal: Poultry Science

Article Title: DL -methionine and DL -methionyl- DL -methionine increase intestinal development and activate Wnt/ β -catenin signaling activity in domestic pigeons ( Columba livia )

doi: 10.1016/j.psj.2021.101644

Figure Lengend Snippet: DL -methionine and DL -methionyl- DL -methionine increase abundance of FZD7 and activate Wnt/β-catenin signaling pathway in pigeon jejunal tissue. (A) The expression of Wnt/β-catenin signaling pathway-associated proteins in the jejunum of pigeons with DL -Met or DL -Met-Met. (B) Statistical analysis of the expression of target proteins based on the images shown in (A). (C) Representative images of IF staining with FZD7 antibodies of the pigeon jejunum (100 ×). Scale bar = 100 μm. (D) Statistical analysis of the expression of FZD7 based on the images shown in (C). (E, F) Binding mode of DL - Met to FZD7 (E) and DL -Met - Met to FZD7 (F). The protein is shown in cartoon form. Surrounding residues are represented in stick form. The dashed lines indicate the formation of hydrogen bonds. The numbers beside the dash show the distance (Å) of hydrogen bonds. “a-c” indicates significant differences ( P < 0.05). The values are mean ±S.E.M. with n = 3.

Article Snippet: Briefly, the samples were blocked in 5% BSA for 2 h and incubated with primary antibodies against ZO-1 (#bs-1329R, Bioss), Ki67 (#NB500-170, Novus Biologicals, Littleton, CO), PCNA (#2586, CST, Danvers, MA), Villin (#SC-58897, Santa Cruz), FZD7 (#bs-5125R, Bioss) and PepT1 (#SC-373742, Santa Cruz) overnight at 4°C and with FITC-(#115-545-003, Jackson Laboratory, Jackson, MS) or Cy3-conjugated secondary antibodies (#111-165-045, Jackson Laboratory) for 2 h at room temperature.

Techniques: Expressing, Staining, Binding Assay

Integrated model depicts the mechanism by which DL -methionine and DL -methionyl- DL -methionine intensify FZD7-mediated Wnt/β-catenin signaling activity and the pathway involved in the cellular uptake of DL -methionyl- DL -methionine mediated by PepT1 in the brush border membrane of enterocytes.

Journal: Poultry Science

Article Title: DL -methionine and DL -methionyl- DL -methionine increase intestinal development and activate Wnt/ β -catenin signaling activity in domestic pigeons ( Columba livia )

doi: 10.1016/j.psj.2021.101644

Figure Lengend Snippet: Integrated model depicts the mechanism by which DL -methionine and DL -methionyl- DL -methionine intensify FZD7-mediated Wnt/β-catenin signaling activity and the pathway involved in the cellular uptake of DL -methionyl- DL -methionine mediated by PepT1 in the brush border membrane of enterocytes.

Article Snippet: Briefly, the samples were blocked in 5% BSA for 2 h and incubated with primary antibodies against ZO-1 (#bs-1329R, Bioss), Ki67 (#NB500-170, Novus Biologicals, Littleton, CO), PCNA (#2586, CST, Danvers, MA), Villin (#SC-58897, Santa Cruz), FZD7 (#bs-5125R, Bioss) and PepT1 (#SC-373742, Santa Cruz) overnight at 4°C and with FITC-(#115-545-003, Jackson Laboratory, Jackson, MS) or Cy3-conjugated secondary antibodies (#111-165-045, Jackson Laboratory) for 2 h at room temperature.

Techniques: Activity Assay