Journal: Nature Communications

Article Title: Deep learning-assisted discovery of a potent and cell-active inhibitor of RNA N 6 -methyladenosine recognition protein YTHDC2

doi: 10.1038/s41467-025-65542-0

Figure Lengend Snippet: a Western blot analyses of H3K4me3 expression in DC2-C1 -treated PANC-1 or MiaPACA-2 cells. Blots shown are representative of three biological replicates. Full blots can be found in Supplementary Fig. . b Quantitative analysis of Western blot for PANC-1 or MiaPACA-2 cells. Data are shown as the mean ± SD from three technical replicates in three independent experiments. c Colony formation assay of PANC-1 or MiaPACA-2 cells treated with DC2-C1 or 16o . d Abilities of invasion of PANC-1 or MiaPACA-2 cells treated with DC2-C1 or 16o . Scale bar, 50 μm. e-g ACC1 , FASN and SREBP-1C mRNA expression were determined by RT-qPCR in DC2-C1 -treated HepG2 cells. Data are shown as the mean ± SD from three technical replicates in three independent experiments. h Western blot analyses of ID2 expression in DC2-C1 -treated PANC-1 cells; Western blot analyses of FZD7 expression in DC2-C1 -treated HGC27 cells. Blots shown are representative of three biological replicates. Full blots can be found in Supplementary Fig. . i FZD7 mRNA expression was determined by RT-qPCR in DC2-C1 -treated HGC27 cells. Data are shown as the mean ± SD from four technical replicates in four independent experiments. j ID2 mRNA expression was validated by RT-qPCR in DC2-C1 -treated PANC-1 cells. Data are shown as the mean ± SD from four technical replicates in four independent experiments. Source data are provided as a Source Data file. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; by two-tailed unpaired Student's t-test).

Article Snippet: Antibodies used: YTHDC2 antibody (ZenBio, R27443 ), AMIGO2 antibody (ImmunoWay, YN2372), ID2 antibody (HUABIO, M1301-2), H3K4me3 antibody (Abcam, ab8580), FZD7 antibody (Proteintech, 16974-1-AP), DYKDDDDK Tag (Flag-tag) antibody (Cell Signaling Technology, 14793), GAPDH antibody (Proteintech, 60004-1-Ig), HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) (Proteintech, SA00001-2), HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (Proteintech, SA00001-1).

Techniques: Western Blot, Expressing, Colony Assay, Quantitative RT-PCR, Two Tailed Test

Journal: Scientific Reports

Article Title: Identification of FZD7 as a potential ferroptosis-related diagnostic gene in endometriosis by bioinformatics analysis

doi: 10.1038/s41598-025-90803-9

Figure Lengend Snippet: ( A , C , E , G , I ) FZD7 is significantly up-regulated in GSE11691, GSE7305, GSE5108, GSE23339 and GSE25628 datasets. ( B , D , F , H , J ) The receiver-operating characteristic (ROC) curve and the area under the curve (AUC) curve of FZD7 in GSE11691, GSE7305, GSE5108, GSE23339 and GSE25628 datasets.

Article Snippet: The membrane was blocked with 5% skimmed milk powder solution for 2 h, then incubated with primary antibody against FZD7 (16974-1-AP, Proteintech, China) overnight at 4 °C, followed by incubation with secondary antibody for 2 h at room temperature.

Techniques:

Journal: Scientific Reports

Article Title: Identification of FZD7 as a potential ferroptosis-related diagnostic gene in endometriosis by bioinformatics analysis

doi: 10.1038/s41598-025-90803-9

Figure Lengend Snippet: GO and KEGG pathways for the overlapping FZD7 positively associated genes in GSE11691, GSE7305, GSE5108, GSE23339 and GSE25628 datasets.

Article Snippet: The membrane was blocked with 5% skimmed milk powder solution for 2 h, then incubated with primary antibody against FZD7 (16974-1-AP, Proteintech, China) overnight at 4 °C, followed by incubation with secondary antibody for 2 h at room temperature.

Techniques:

Journal: Scientific Reports

Article Title: Identification of FZD7 as a potential ferroptosis-related diagnostic gene in endometriosis by bioinformatics analysis

doi: 10.1038/s41598-025-90803-9

Figure Lengend Snippet: ( A , C ) The infiltration of 22 immune cells in EMS of GSE11691dataset. ( B ) The relationship between FZD7 and 22 immune cells in GSE11691 dataset. ( D ) The expression of FZD7 in GSE19834. ( E ) Immunofluorescence images staining with CD206 in Vehicle group and F7H group.

Article Snippet: The membrane was blocked with 5% skimmed milk powder solution for 2 h, then incubated with primary antibody against FZD7 (16974-1-AP, Proteintech, China) overnight at 4 °C, followed by incubation with secondary antibody for 2 h at room temperature.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Scientific Reports

Article Title: Identification of FZD7 as a potential ferroptosis-related diagnostic gene in endometriosis by bioinformatics analysis

doi: 10.1038/s41598-025-90803-9

Figure Lengend Snippet: ( A-C ) The expression of FZD7 in ectopic (Ec) endometrial tissues, eutopic (Eu) endometrial tissues and normal endometrial (NE) tissues. ** p < 0.01, *** p < 0.001, ns, no significant difference by one-way analysis of variance (ANOVA) test.

Article Snippet: The membrane was blocked with 5% skimmed milk powder solution for 2 h, then incubated with primary antibody against FZD7 (16974-1-AP, Proteintech, China) overnight at 4 °C, followed by incubation with secondary antibody for 2 h at room temperature.

Techniques: Expressing