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  • 98
    Millipore monoclonal anti brdu antibody
    Monoclonal Anti Brdu Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    BioreclamationIVT anti bru
    Double transfection of BrUTP and the antisense H5R oligonucleotide at different times of infection reveals two different structures that accumulate viral mRNAs in infected cells. HeLa cells were infected and transfected with BrUTP for 2 h in the presence of 2 mM HU. HU was washed out, and cells were transfected with the antisense oligonucleotide corresponding to H5R mRNA. Double-transfected cells were fixed 2 h after HU washout and triple labeled with <t>anti-BrU</t> (FITC) (yellow channel), streptavidin-rhodamine (H5R) (red channel), and <t>DAPI</t> (bluechannel). The solid arrows in all four panels indicate DAPI-positive replication sites, the labeling of which entirely colocalizes with the pattern of the H5R oligonucleotide. The BrU pattern, indicated by hatched arrows (BrU and merge panels), does not colocalize with either of the two labels. It accumulates in granular structures that appear to have no relationship with the replication sites. This is most dramatically shown in the upper left cell, in which the replication sites all seem to collect at the upper left side of the cell, while the BrU-positive structures accumulate at the lower right side of the same cell. Bar, 10 μm.
    Anti Bru, supplied by BioreclamationIVT, used in various techniques. Bioz Stars score: 88/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore bru
    Effect of O 3 exposure on <t>RNA</t> transcription: representative transmission electron micrographs of CTR (a) and 10 µg O 3 /mL-treated cells (b) labelled for <t>BrU;</t> BrU molecules have been incorporated in perichromatin fibrils (arrows) and in the nucleolar dense fibrillar component (arrowheads); Nu, nucleolus; scale bar: 500 nm. c,d) Mean±SE values of BrU labelling density evaluated on nucleoplasm and nucleoli of cells incubated with BrU 24 h post-treatment; asterisks indicate values significantly different from each other.
    Bru, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher bru
    RNA is associated with DNA at all stages of mitosis. ( a ) Proximity of 5-bromouridine <t>(BrU)-labeled</t> RNA and 5-ethynyl-2′-deoxyuridine <t>(EdU)-labeled</t> DNA was assessed by RNA–DNA Interaction Assay (RDIA). Following labeling with BrU, embryos were either untreated (left) or treated (right) with RNase A. PLA, red; EdU, green; 4,6-diamidino-2-phenylindole (DAPI), blue. ( b ) Association of RNA with DNA at all mitotic stages was determined by RDIA. Black and white bottom panels show PLA signals only. PLA, red; EdU, green; H3S10-p, blue.
    Bru, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Santa Cruz Biotechnology anti bru agarose beads
    RNA is associated with DNA at all stages of mitosis. ( a ) Proximity of 5-bromouridine <t>(BrU)-labeled</t> RNA and 5-ethynyl-2′-deoxyuridine <t>(EdU)-labeled</t> DNA was assessed by RNA–DNA Interaction Assay (RDIA). Following labeling with BrU, embryos were either untreated (left) or treated (right) with RNase A. PLA, red; EdU, green; 4,6-diamidino-2-phenylindole (DAPI), blue. ( b ) Association of RNA with DNA at all mitotic stages was determined by RDIA. Black and white bottom panels show PLA signals only. PLA, red; EdU, green; H3S10-p, blue.
    Anti Bru Agarose Beads, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Applied Photophysics brus l luminescence photophysics
    RNA is associated with DNA at all stages of mitosis. ( a ) Proximity of 5-bromouridine <t>(BrU)-labeled</t> RNA and 5-ethynyl-2′-deoxyuridine <t>(EdU)-labeled</t> DNA was assessed by RNA–DNA Interaction Assay (RDIA). Following labeling with BrU, embryos were either untreated (left) or treated (right) with RNase A. PLA, red; EdU, green; 4,6-diamidino-2-phenylindole (DAPI), blue. ( b ) Association of RNA with DNA at all mitotic stages was determined by RDIA. Black and white bottom panels show PLA signals only. PLA, red; EdU, green; H3S10-p, blue.
    Brus L Luminescence Photophysics, supplied by Applied Photophysics, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Schiff Nutrition International bru
    RNA is associated with DNA at all stages of mitosis. ( a ) Proximity of 5-bromouridine <t>(BrU)-labeled</t> RNA and 5-ethynyl-2′-deoxyuridine <t>(EdU)-labeled</t> DNA was assessed by RNA–DNA Interaction Assay (RDIA). Following labeling with BrU, embryos were either untreated (left) or treated (right) with RNase A. PLA, red; EdU, green; 4,6-diamidino-2-phenylindole (DAPI), blue. ( b ) Association of RNA with DNA at all mitotic stages was determined by RDIA. Black and white bottom panels show PLA signals only. PLA, red; EdU, green; H3S10-p, blue.
    Bru, supplied by Schiff Nutrition International, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millegen rabbit anti bru 3
    stj-RC transcript isoform carrying long 3’UTR and alternatively spliced exon 15 is up regulated in Hand > MblRNAi and in Hand > <t>Bru-3</t> hearts. ( A ) RT-qPCR probes for stj-RC specific exon 15 and for total stj transcripts are indicated on the scheme. ( B ) RT-qPCR analysis of stj-RC and total stj transcript levels in control ( Hand > lacZ ) fly hearts and in two DM1 contexts ( Hand > MblRNAi and Hand > Bru-3 ). One-way ANOVA, Kruskall-wallis Dunn’s multiple comparison post-test was applied for assessing statistical significance (* - p
    Rabbit Anti Bru 3, supplied by Millegen, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti bru antibody
    stj-RC transcript isoform carrying long 3’UTR and alternatively spliced exon 15 is up regulated in Hand > MblRNAi and in Hand > <t>Bru-3</t> hearts. ( A ) RT-qPCR probes for stj-RC specific exon 15 and for total stj transcripts are indicated on the scheme. ( B ) RT-qPCR analysis of stj-RC and total stj transcript levels in control ( Hand > lacZ ) fly hearts and in two DM1 contexts ( Hand > MblRNAi and Hand > Bru-3 ). One-way ANOVA, Kruskall-wallis Dunn’s multiple comparison post-test was applied for assessing statistical significance (* - p
    Anti Bru Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MBL International anti bru antibody
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Anti Bru Antibody, supplied by MBL International, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher anti bru antibody
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Anti Bru Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioreclamationIVT anti bru monoclonal antibody
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Anti Bru Monoclonal Antibody, supplied by BioreclamationIVT, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti bru beads
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Anti Bru Beads, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam bru antibody
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Bru Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore bru sulfate salt hydrate
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Bru Sulfate Salt Hydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Exbio Praha bru
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Bru, supplied by Exbio Praha, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson mouse anti bru antibody
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Mouse Anti Bru Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioreclamationIVT rat anti bru antibodies
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Rat Anti Bru Antibodies, supplied by BioreclamationIVT, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson moflo bru cell sorter
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Moflo Bru Cell Sorter, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    biomers.net 5 bromo uracil 5 bru
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    5 Bromo Uracil 5 Bru, supplied by biomers.net, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti bru antibodies conjugated magnetic beads
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Anti Bru Antibodies Conjugated Magnetic Beads, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti bru
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Anti Bru, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti brdu bru primary antibodies
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Anti Brdu Bru Primary Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti brdu bru
    Analysis of binding region of YY1 and EZH2 in <t>TUG1</t> RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with <t>BrU-labelled</t> partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P
    Anti Brdu Bru, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Double transfection of BrUTP and the antisense H5R oligonucleotide at different times of infection reveals two different structures that accumulate viral mRNAs in infected cells. HeLa cells were infected and transfected with BrUTP for 2 h in the presence of 2 mM HU. HU was washed out, and cells were transfected with the antisense oligonucleotide corresponding to H5R mRNA. Double-transfected cells were fixed 2 h after HU washout and triple labeled with anti-BrU (FITC) (yellow channel), streptavidin-rhodamine (H5R) (red channel), and DAPI (bluechannel). The solid arrows in all four panels indicate DAPI-positive replication sites, the labeling of which entirely colocalizes with the pattern of the H5R oligonucleotide. The BrU pattern, indicated by hatched arrows (BrU and merge panels), does not colocalize with either of the two labels. It accumulates in granular structures that appear to have no relationship with the replication sites. This is most dramatically shown in the upper left cell, in which the replication sites all seem to collect at the upper left side of the cell, while the BrU-positive structures accumulate at the lower right side of the same cell. Bar, 10 μm.

    Journal: Journal of Virology

    Article Title: Relationship between Vaccinia Virus Intracellular Cores, Early mRNAs, and DNA Replication Sites

    doi: 10.1128/JVI.76.10.5167-5183.2002

    Figure Lengend Snippet: Double transfection of BrUTP and the antisense H5R oligonucleotide at different times of infection reveals two different structures that accumulate viral mRNAs in infected cells. HeLa cells were infected and transfected with BrUTP for 2 h in the presence of 2 mM HU. HU was washed out, and cells were transfected with the antisense oligonucleotide corresponding to H5R mRNA. Double-transfected cells were fixed 2 h after HU washout and triple labeled with anti-BrU (FITC) (yellow channel), streptavidin-rhodamine (H5R) (red channel), and DAPI (bluechannel). The solid arrows in all four panels indicate DAPI-positive replication sites, the labeling of which entirely colocalizes with the pattern of the H5R oligonucleotide. The BrU pattern, indicated by hatched arrows (BrU and merge panels), does not colocalize with either of the two labels. It accumulates in granular structures that appear to have no relationship with the replication sites. This is most dramatically shown in the upper left cell, in which the replication sites all seem to collect at the upper left side of the cell, while the BrU-positive structures accumulate at the lower right side of the same cell. Bar, 10 μm.

    Article Snippet: Fixed cells were then either double labeled with anti-BrU and DAPI or triple labeled with anti-BrU (followed by an anti-rat antibody coupled to rhodamine), DAPI, and streptavidin-FITC.

    Techniques: Transfection, Infection, Labeling

    Intracellular cores and the sites of early mRNA accumulation do not colocalize. HeLa cells grown on coverslips were infected and transfected with BrUTP as described in Materials and Methods in the presence of 5 mM HU in order to block DNA replication. Cells were fixed at 2 h postinfection and double labeled with the anti-core antibody, followed by donkey anti-rabbit coupled to rhodamine, and anti-BrU, followed by goat anti-rat-FITC. The merge in the bottom panel shows that the two structures do not overlap at all. Bar, 10 μm.

    Journal: Journal of Virology

    Article Title: Relationship between Vaccinia Virus Intracellular Cores, Early mRNAs, and DNA Replication Sites

    doi: 10.1128/JVI.76.10.5167-5183.2002

    Figure Lengend Snippet: Intracellular cores and the sites of early mRNA accumulation do not colocalize. HeLa cells grown on coverslips were infected and transfected with BrUTP as described in Materials and Methods in the presence of 5 mM HU in order to block DNA replication. Cells were fixed at 2 h postinfection and double labeled with the anti-core antibody, followed by donkey anti-rabbit coupled to rhodamine, and anti-BrU, followed by goat anti-rat-FITC. The merge in the bottom panel shows that the two structures do not overlap at all. Bar, 10 μm.

    Article Snippet: Fixed cells were then either double labeled with anti-BrU and DAPI or triple labeled with anti-BrU (followed by an anti-rat antibody coupled to rhodamine), DAPI, and streptavidin-FITC.

    Techniques: Infection, Transfection, Blocking Assay, Labeling

    Effect of O 3 exposure on RNA transcription: representative transmission electron micrographs of CTR (a) and 10 µg O 3 /mL-treated cells (b) labelled for BrU; BrU molecules have been incorporated in perichromatin fibrils (arrows) and in the nucleolar dense fibrillar component (arrowheads); Nu, nucleolus; scale bar: 500 nm. c,d) Mean±SE values of BrU labelling density evaluated on nucleoplasm and nucleoli of cells incubated with BrU 24 h post-treatment; asterisks indicate values significantly different from each other.

    Journal: European Journal of Histochemistry : EJH

    Article Title: Low Ozone Concentrations Stimulate Cytoskeletal Organization, Mitochondrial Activity and Nuclear Transcription

    doi: 10.4081/ejh.2015.2515

    Figure Lengend Snippet: Effect of O 3 exposure on RNA transcription: representative transmission electron micrographs of CTR (a) and 10 µg O 3 /mL-treated cells (b) labelled for BrU; BrU molecules have been incorporated in perichromatin fibrils (arrows) and in the nucleolar dense fibrillar component (arrowheads); Nu, nucleolus; scale bar: 500 nm. c,d) Mean±SE values of BrU labelling density evaluated on nucleoplasm and nucleoli of cells incubated with BrU 24 h post-treatment; asterisks indicate values significantly different from each other.

    Article Snippet: In order to evaluate RNA transcription rate, some cells were pulse-labelled with 10 mM BrU (5-bromouridine, Sigma-Aldrich) for 10 min at 37°C, and then fixed and processed for immunoelectron microscopy as above.

    Techniques: Transmission Assay, Incubation

    RNA is associated with DNA at all stages of mitosis. ( a ) Proximity of 5-bromouridine (BrU)-labeled RNA and 5-ethynyl-2′-deoxyuridine (EdU)-labeled DNA was assessed by RNA–DNA Interaction Assay (RDIA). Following labeling with BrU, embryos were either untreated (left) or treated (right) with RNase A. PLA, red; EdU, green; 4,6-diamidino-2-phenylindole (DAPI), blue. ( b ) Association of RNA with DNA at all mitotic stages was determined by RDIA. Black and white bottom panels show PLA signals only. PLA, red; EdU, green; H3S10-p, blue.

    Journal: Cell Discovery

    Article Title: Chromatin proteins and RNA are associated with DNA during all phases of mitosis

    doi: 10.1038/celldisc.2016.38

    Figure Lengend Snippet: RNA is associated with DNA at all stages of mitosis. ( a ) Proximity of 5-bromouridine (BrU)-labeled RNA and 5-ethynyl-2′-deoxyuridine (EdU)-labeled DNA was assessed by RNA–DNA Interaction Assay (RDIA). Following labeling with BrU, embryos were either untreated (left) or treated (right) with RNase A. PLA, red; EdU, green; 4,6-diamidino-2-phenylindole (DAPI), blue. ( b ) Association of RNA with DNA at all mitotic stages was determined by RDIA. Black and white bottom panels show PLA signals only. PLA, red; EdU, green; H3S10-p, blue.

    Article Snippet: For RNase experiment, embryos were labeled with EdU, chased as described above and then incubated with 2 mM BrU (Acros Organics) in Drosophila Schneider medium for 5 min and Triton was added to final concentration 0.2% for additional 10 min of incubation.

    Techniques: Labeling, Proximity Ligation Assay

    stj-RC transcript isoform carrying long 3’UTR and alternatively spliced exon 15 is up regulated in Hand > MblRNAi and in Hand > Bru-3 hearts. ( A ) RT-qPCR probes for stj-RC specific exon 15 and for total stj transcripts are indicated on the scheme. ( B ) RT-qPCR analysis of stj-RC and total stj transcript levels in control ( Hand > lacZ ) fly hearts and in two DM1 contexts ( Hand > MblRNAi and Hand > Bru-3 ). One-way ANOVA, Kruskall-wallis Dunn’s multiple comparison post-test was applied for assessing statistical significance (* - p

    Journal: eLife

    Article Title: Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1

    doi: 10.7554/eLife.51114

    Figure Lengend Snippet: stj-RC transcript isoform carrying long 3’UTR and alternatively spliced exon 15 is up regulated in Hand > MblRNAi and in Hand > Bru-3 hearts. ( A ) RT-qPCR probes for stj-RC specific exon 15 and for total stj transcripts are indicated on the scheme. ( B ) RT-qPCR analysis of stj-RC and total stj transcript levels in control ( Hand > lacZ ) fly hearts and in two DM1 contexts ( Hand > MblRNAi and Hand > Bru-3 ). One-way ANOVA, Kruskall-wallis Dunn’s multiple comparison post-test was applied for assessing statistical significance (* - p

    Article Snippet: The following primary antibodies were used: sheep anti-Mbl antibody (1:200, kindly provided by Darren Monckton), rabbit anti-Bru-3 (1:1000; Millegen, Toulouse, France), rabbit anti-Stj (1:500; kindly provided by Hugo Bellen), rabbit anti-α2δ3 (1:200, gift of Greg Neely) and goat anti-GFP (1:500, Abcam, ab 5450).

    Techniques: Quantitative RT-PCR

    Stj protein levels in different genetic contexts visualized by immunostaining. Stj protein expression (red) in circular fibers of cardiomyocytes in wild-type (wt) ( A ) and in different genetic contexts ( B-E ) tested. Circular fibers are visualized with phalloidin staining (green). Notice that Stj protein levels appear higher in Hand > Bru-3 and in Hand > MblRNAi compared to wt. High signal level in Hand > stj and loss of signal in Hand > stjRNAi demonstrate also the specificity of stj antibody. Scale bars, 50 μm. ( F ) Corrected total cell fluorescence (CTCF) of Stj signal in circular fibers of cardiomyocytes ( Figure 5—source data 1 ) in different genetic contexts measured using Image J according to Burgess et al. (2010) . Statistical significance (Kruskal-Wallis test) denoted by ns (p > 0.05) * (p

    Journal: eLife

    Article Title: Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1

    doi: 10.7554/eLife.51114

    Figure Lengend Snippet: Stj protein levels in different genetic contexts visualized by immunostaining. Stj protein expression (red) in circular fibers of cardiomyocytes in wild-type (wt) ( A ) and in different genetic contexts ( B-E ) tested. Circular fibers are visualized with phalloidin staining (green). Notice that Stj protein levels appear higher in Hand > Bru-3 and in Hand > MblRNAi compared to wt. High signal level in Hand > stj and loss of signal in Hand > stjRNAi demonstrate also the specificity of stj antibody. Scale bars, 50 μm. ( F ) Corrected total cell fluorescence (CTCF) of Stj signal in circular fibers of cardiomyocytes ( Figure 5—source data 1 ) in different genetic contexts measured using Image J according to Burgess et al. (2010) . Statistical significance (Kruskal-Wallis test) denoted by ns (p > 0.05) * (p

    Article Snippet: The following primary antibodies were used: sheep anti-Mbl antibody (1:200, kindly provided by Darren Monckton), rabbit anti-Bru-3 (1:1000; Millegen, Toulouse, France), rabbit anti-Stj (1:500; kindly provided by Hugo Bellen), rabbit anti-α2δ3 (1:200, gift of Greg Neely) and goat anti-GFP (1:500, Abcam, ab 5450).

    Techniques: Immunostaining, Expressing, Staining, Fluorescence

    Cardiac-specific transcriptional profiling using TU-tagging method. ( A ) Pipeline of heart-targeted transcriptional profiling using TU-tagging. Note that Gal4- inducible UPRT transgene ( UAS-UPRT ) has been combined with UAS-MblRNAi and with UAS-Bru-3 for the purpose of TU-tagging. Hand > UPRT;lacZ is the control line used to identify pathogenic gene deregulations in Hand > UPRT;MblRNAi and Hand > UPRT;Bru-3 contexts. Flies were starved at day 4 for 6 hr before being transferred to 4TU containing food for 12 hr. ( B ) Volcano plot and IGV tracks from control Hand > UPRT;lacZ flies show examples of enrichment of heart-specific genes (e.g. Hand , Tin ) (red, right side) and depletion of non-heart-expressed genes (blue, left side), thus validating the specificity of heart targeting.

    Journal: eLife

    Article Title: Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1

    doi: 10.7554/eLife.51114

    Figure Lengend Snippet: Cardiac-specific transcriptional profiling using TU-tagging method. ( A ) Pipeline of heart-targeted transcriptional profiling using TU-tagging. Note that Gal4- inducible UPRT transgene ( UAS-UPRT ) has been combined with UAS-MblRNAi and with UAS-Bru-3 for the purpose of TU-tagging. Hand > UPRT;lacZ is the control line used to identify pathogenic gene deregulations in Hand > UPRT;MblRNAi and Hand > UPRT;Bru-3 contexts. Flies were starved at day 4 for 6 hr before being transferred to 4TU containing food for 12 hr. ( B ) Volcano plot and IGV tracks from control Hand > UPRT;lacZ flies show examples of enrichment of heart-specific genes (e.g. Hand , Tin ) (red, right side) and depletion of non-heart-expressed genes (blue, left side), thus validating the specificity of heart targeting.

    Article Snippet: The following primary antibodies were used: sheep anti-Mbl antibody (1:200, kindly provided by Darren Monckton), rabbit anti-Bru-3 (1:1000; Millegen, Toulouse, France), rabbit anti-Stj (1:500; kindly provided by Hugo Bellen), rabbit anti-α2δ3 (1:200, gift of Greg Neely) and goat anti-GFP (1:500, Abcam, ab 5450).

    Techniques:

    Bru-3 and Mbl are expressed in the adult fly heart and their transcript and protein levels are affected in Hand > Bru -3 and Hand > MblRNAi flies. ( A–B ) RT-qPCR analyses of Bru-3 overexpression and Mbl attenuation in the adult hearts from Hand > Bru-3 ( A ) and Hand > MblRNAi ( B ) flies. Three biological replicates (n = 10–12 hearts per replicate) were analysed for each condition. Statistical significance (Mann Whitney U test) is denoted by * (p

    Journal: eLife

    Article Title: Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1

    doi: 10.7554/eLife.51114

    Figure Lengend Snippet: Bru-3 and Mbl are expressed in the adult fly heart and their transcript and protein levels are affected in Hand > Bru -3 and Hand > MblRNAi flies. ( A–B ) RT-qPCR analyses of Bru-3 overexpression and Mbl attenuation in the adult hearts from Hand > Bru-3 ( A ) and Hand > MblRNAi ( B ) flies. Three biological replicates (n = 10–12 hearts per replicate) were analysed for each condition. Statistical significance (Mann Whitney U test) is denoted by * (p

    Article Snippet: The following primary antibodies were used: sheep anti-Mbl antibody (1:200, kindly provided by Darren Monckton), rabbit anti-Bru-3 (1:1000; Millegen, Toulouse, France), rabbit anti-Stj (1:500; kindly provided by Hugo Bellen), rabbit anti-α2δ3 (1:200, gift of Greg Neely) and goat anti-GFP (1:500, Abcam, ab 5450).

    Techniques: Quantitative RT-PCR, Over Expression, MANN-WHITNEY

    The expression levels of CG42617 and CG16868 , two additional α2δ protein-coding genes are not affected in the heart of DM1 fly models. Normalized RNAseq IGV tracks in control ( Hand > lacZ ) and in pathogenic DM1 contexts ( Hand > MblRNAi and Hand > Bru-3 ) are shown aligned with genomic exon/intron organization of CG42617 ( A ) and CG16868 ( B ).

    Journal: eLife

    Article Title: Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1

    doi: 10.7554/eLife.51114

    Figure Lengend Snippet: The expression levels of CG42617 and CG16868 , two additional α2δ protein-coding genes are not affected in the heart of DM1 fly models. Normalized RNAseq IGV tracks in control ( Hand > lacZ ) and in pathogenic DM1 contexts ( Hand > MblRNAi and Hand > Bru-3 ) are shown aligned with genomic exon/intron organization of CG42617 ( A ) and CG16868 ( B ).

    Article Snippet: The following primary antibodies were used: sheep anti-Mbl antibody (1:200, kindly provided by Darren Monckton), rabbit anti-Bru-3 (1:1000; Millegen, Toulouse, France), rabbit anti-Stj (1:500; kindly provided by Hugo Bellen), rabbit anti-α2δ3 (1:200, gift of Greg Neely) and goat anti-GFP (1:500, Abcam, ab 5450).

    Techniques: Expressing

    Effect of simultaneous cardiac attenuation of Mbl and overexpression of Bru-3 on conduction defects. Barplot graph showing percentage of flies with conduction defects. Note a moderate additive effect of simultaneous attenuation of Mbl and overexpression of Bru-3. Number of fly hearts tested (n) is indicated and statistical significance (Fisher’s exact test) denoted by ns (p > 0.05) and *** (p

    Journal: eLife

    Article Title: Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1

    doi: 10.7554/eLife.51114

    Figure Lengend Snippet: Effect of simultaneous cardiac attenuation of Mbl and overexpression of Bru-3 on conduction defects. Barplot graph showing percentage of flies with conduction defects. Note a moderate additive effect of simultaneous attenuation of Mbl and overexpression of Bru-3. Number of fly hearts tested (n) is indicated and statistical significance (Fisher’s exact test) denoted by ns (p > 0.05) and *** (p

    Article Snippet: The following primary antibodies were used: sheep anti-Mbl antibody (1:200, kindly provided by Darren Monckton), rabbit anti-Bru-3 (1:1000; Millegen, Toulouse, France), rabbit anti-Stj (1:500; kindly provided by Hugo Bellen), rabbit anti-α2δ3 (1:200, gift of Greg Neely) and goat anti-GFP (1:500, Abcam, ab 5450).

    Techniques: Over Expression

    Analysis of binding region of YY1 and EZH2 in TUG1 RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with BrU-labelled partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P

    Journal: Nature Communications

    Article Title: Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment

    doi: 10.1038/ncomms13616

    Figure Lengend Snippet: Analysis of binding region of YY1 and EZH2 in TUG1 RNA. ( a ) Schematic diagram of deletion fragments of TUG1 exon 2 RNA. The binding site of miR-145 is indicated (arrowhead). ( b ) RNA pull-down assay with BrU-labelled partial TUG1 RNAs as indicated in a . Immunoprecipitated fractions with anti-BrU antibody were analysed by western blotting using anti-EZH2 and anti-YY1 antibodies (upper). EGFP - ORF RNA is used as a negative control. Gel electrophoresis image of BrU-labelled RNAs is shown (bottom). ( c ) Expression level of TUG1 in GSCs and serum-differentiated GSCs. Relative expression level compared to that in GSCs is indicated on the y -axis. * P

    Article Snippet: Chromatin and BrU-labelled TUG1 RNA mixtures were immunoprecipitated using an anti-BrU antibody and Dynabeads Protein G. Purified chromatin was eluted to yield DNA, which was then subjected to microarray analysis or ChIP-qPCR analysis.

    Techniques: Binding Assay, Pull Down Assay, Immunoprecipitation, Western Blot, Negative Control, Nucleic Acid Electrophoresis, Expressing

    Suppression of neuronal differentiation-associated genes by the TUG1 -PRC2 complex. ( a ) RNA pull-down analysis with anti-BrU antibody. Nuclear extracts from GSCs were incubated with TUG1 RNA labelled with BrU (BrU+) or without (BrU-). TUG1 -RNA-binding proteins were analysed by western blotting. Input extract is used as control. ( b ) Nuclear extracts from GSCs treated with either si- NC or si- TUG1 were immunoprecipitated with anti-YY1 antibody. The immunoprecipitated fractions and lysate aliquots (Input) were subjected to western blotting. A part of the nuclear extract from si- NC- treated cells was also treated with RNase, immunoprecipitated with anti-YY1 antibody, and analysed by western blotting (RNase, left). Relative intensity values of EZH2 normalized to Input are indicated in ( c ). ( d ) Venn diagram shows relationship between TUG1 target genes (630 genes) and 1,714 genes that contain a YY1 motif around their TSS. Among 630 TUG1 target genes, 525 genes contain a YY1 motif. ( e ) Heatmap indicates expression changes of 525 TUG1 target genes containing a YY1 motif (shown in d ) upon inhibition of TUG1 (si- TUG1 #1 and #2). Colour corresponds to expression level as indicated in the log 2 -transformed scale bar below the matrix. Red and blue reflect high and low levels, respectively. ( f ) Averaged expression value of 525 genes shown in ( e ). Error bars indicate s.e.m. * P

    Journal: Nature Communications

    Article Title: Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment

    doi: 10.1038/ncomms13616

    Figure Lengend Snippet: Suppression of neuronal differentiation-associated genes by the TUG1 -PRC2 complex. ( a ) RNA pull-down analysis with anti-BrU antibody. Nuclear extracts from GSCs were incubated with TUG1 RNA labelled with BrU (BrU+) or without (BrU-). TUG1 -RNA-binding proteins were analysed by western blotting. Input extract is used as control. ( b ) Nuclear extracts from GSCs treated with either si- NC or si- TUG1 were immunoprecipitated with anti-YY1 antibody. The immunoprecipitated fractions and lysate aliquots (Input) were subjected to western blotting. A part of the nuclear extract from si- NC- treated cells was also treated with RNase, immunoprecipitated with anti-YY1 antibody, and analysed by western blotting (RNase, left). Relative intensity values of EZH2 normalized to Input are indicated in ( c ). ( d ) Venn diagram shows relationship between TUG1 target genes (630 genes) and 1,714 genes that contain a YY1 motif around their TSS. Among 630 TUG1 target genes, 525 genes contain a YY1 motif. ( e ) Heatmap indicates expression changes of 525 TUG1 target genes containing a YY1 motif (shown in d ) upon inhibition of TUG1 (si- TUG1 #1 and #2). Colour corresponds to expression level as indicated in the log 2 -transformed scale bar below the matrix. Red and blue reflect high and low levels, respectively. ( f ) Averaged expression value of 525 genes shown in ( e ). Error bars indicate s.e.m. * P

    Article Snippet: Chromatin and BrU-labelled TUG1 RNA mixtures were immunoprecipitated using an anti-BrU antibody and Dynabeads Protein G. Purified chromatin was eluted to yield DNA, which was then subjected to microarray analysis or ChIP-qPCR analysis.

    Techniques: Incubation, RNA Binding Assay, Western Blot, Immunoprecipitation, Expressing, Inhibition, Transformation Assay